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Página 18 dos resultados de 3665068 itens digitais encontrados em 1.140 segundos

Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana

Tomiyama, Masakazu; Inoue, Shin-ichiro; Tsuzuki, Tomo; Soda, Midori; Morimoto, Sayuri; Okigaki, Yukiko; Ohishi, Takaya; Mochizuki, Nobuyoshi; Takahashi, Koji; Kinoshita, Toshinori
Fonte: Springer Japan Publicador: Springer Japan
Tipo: Text
Português
To elucidate the molecular mechanisms of stomatal opening and closure, we performed a genetic screen using infrared thermography to isolate stomatal aperture mutants. We identified a mutant designated low temperature with open-stomata 1 (lost1), which exhibited reduced leaf temperature, wider stomatal aperture, and a pale green phenotype. Map-based analysis of the LOST1 locus revealed that the lost1 mutant resulted from a missense mutation in the Mg-chelatase I subunit 1 (CHLI1) gene, which encodes a subunit of the Mg-chelatase complex involved in chlorophyll synthesis. Transformation of the wild-type CHLI1 gene into lost1 complemented all lost1 phenotypes. Stomata in lost1 exhibited a partial ABA-insensitive phenotype similar to that of rtl1, a Mg-chelatase H subunit missense mutant. The Mg-protoporphyrin IX methyltransferase (CHLM) gene encodes a subsequent enzyme in the chlorophyll synthesis pathway. We examined stomatal movement in a CHLM knockdown mutant, chlm, and found that it also exhibited an ABA-insensitive phenotype. However, lost1 and chlm seedlings all showed normal expression of ABA-induced genes, such as RAB18 and RD29B, in response to ABA. These results suggest that the chlorophyll synthesis enzymes, Mg-chelatase complex and CHLM...

Evidence supporting a critical contribution of intrinsically disordered regions to the biochemical behavior of full-length human HP1γ

Velez, Gabriel; Lin, Marisa; Christensen, Trace; Faubion, William A.; Lomberk, Gwen; Urrutia, Raul
Fonte: Springer Berlin Heidelberg Publicador: Springer Berlin Heidelberg
Tipo: Text
Português
HP1γ, a non-histone chromatin protein, has elicited significant attention because of its role in gene silencing, elongation, splicing, DNA repair, cell growth, differentiation, and many other cancer-associated processes, including therapy resistance. These characteristics make it an ideal target for developing small drugs for both mechanistic experimentation and potential therapies. While high-resolution structures of the two globular regions of HP1γ, the chromo- and chromoshadow domains, have been solved, little is currently known about the conformational behavior of the full-length protein. Consequently, in the current study, we use threading, homology-based molecular modeling, molecular mechanics calculations, and molecular dynamics simulations to develop models that allow us to infer properties of full-length HP1γ at an atomic resolution level. HP1γ appears as an elongated molecule in which three Intrinsically Disordered Regions (IDRs, 1, 2, and 3) endow this protein with dynamic flexibility, intermolecular recognition properties, and the ability to integrate signals from various intracellular pathways. Our modeling also suggests that the dynamic flexibility imparted to HP1γ by the three IDRs is important for linking nucleosomes with PXVXL motif-containing proteins...

Functional screening of a cDNA library from the desiccation-tolerant plant Selaginella lepidophylla in yeast mutants identifies trehalose biosynthesis genes of plant and microbial origin

Pampurova, Suzana; Verschooten, Katrien; Avonce, Nelson; Van Dijck, Patrick
Fonte: Springer Japan Publicador: Springer Japan
Tipo: Text
Português
Trehalose is a non-reducing disaccharide that accumulates to large quantities in microbial cells, but in plants it is generally present in very low, barely-detectible levels. A notable exception is the desiccation-tolerant plant Selaginella lepidophylla, which accumulates very high levels of trehalose in both the hydrated and dehydrated state. As trehalose is known to protect membranes, proteins, and whole cells against dehydration stress, we have been interested in the characterization of the trehalose biosynthesis enzymes of S. lepidophylla; they could assist in engineering crop plants towards better stress tolerance. We previously isolated and characterized trehalose-6-phosphate synthases from Arabidopsis thaliana (desiccation sensitive) and S. lepidophylla (desiccation tolerant) and found that they had similar enzymatic characteristics. In this paper, we describe the isolation and characterization of trehalose-6-phosphate phosphatase from S. lepidophylla and show that its catalytic activities are also similar to those of its homolog in A. thaliana. Screening of an S. lepidophylla cDNA library using yeast trehalose biosynthesis mutants resulted in the isolation of a large number of trehalose biosynthesis genes that were of microbial rather than plant origin. Thus...

Whole-Genome Sequences of Novel Porcine Circovirus Type 2 Viruses Detected in Swine from Mexico and the United States

Harmon, Karen M.; Gauger, Phillip C.; Zhang, Jianqiang; Piñeyro, Pablo E.; Dunn, Derek D.; Chriswell, Amy J.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
A unique porcine circovirus type 2 capsid protein (ORF2) sequence was detected in swine samples submitted to the Iowa State University Veterinary Diagnostic Laboratory. The complete genome sequences of four viruses, one from Mexico and three from the United States, were determined to further characterize this novel PCV2 genotype.

Complete Genome Sequence of Mayaro Virus (Togaviridae, Alphavirus) Strain BeAr 20290 from Brazil

Espósito, Danillo Lucas Alves; da Fonseca, Benedito Antônio Lopes
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
We report here the complete genome sequence of Mayaro virus strain BeAr 20290 isolated from Haemagogus mosquitoes in 1960. The sequence presented here includes all nonstructural and structural proteins and the 5′- and 3′-untranslated (UTR) regions.

Genome Sequences of 11 Brucella abortus Isolates from Persistently Infected Italian Regions

Garofolo, Giuliano; Foster, Jeffrey T.; Drees, Kevin; Zilli, Katiuscia; Platone, Ilenia; Ancora, Massimo; Cammà, Cesare; De Massis, Fabrizio; Calistri, Paolo; Di Giannatale, Elisabetta
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Bovine brucellosis, typically caused by Brucella abortus, has been eradicated from much of the developed world. However, the disease remains prevalent in southern Italy, persisting as a public and livestock health concern. We report here the whole-genome sequences of 11 isolates from cattle (Bos taurus) and water buffalo (Bubalus bubalis) that are representative of the current genetic diversity of B. abortus lineages circulating in Italy.

Complete Genome Sequence of Streptococcus anginosus J4211, a Clinical Isolate

Rahman, Maliha; Nguyen, Scott V.; McCullor, Kimberly A.; King, Catherine J.; Jorgensen, James H.; McShan, W. Michael
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Streptococcus anginosus is an opportunistic human pathogen that causes abscesses of the brain, liver, and other organs. Here, we announce the complete genome sequence of a clinically isolated strain of S. anginosus J4211. The genome sequence contains two prophages and multiple mobile genetic elements.

Complete Genome Sequence of Aneurinibacillus migulanus E1, a Gramicidin S- and d-Phenylalanyl-l-Propyl Diketopiperazine-Deficient Mutant

Belbahri, Lassaad; Alenezi, Faizah N.; Luptakova, Lenka; Rateb, Mostafa E.; Woodward, Steve
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
We report here the complete genome sequence of the Aneurinibacillus migulanus E1 mutant deficient in gramicidin S (GS) and d-phenylalanyl-l-propyl diketopiperazine (DKP) formation. The genome consists of a circular chromosome (6,301,904 bp, 43.20% G+C content) without any plasmid. The complete genome sequence enables further investigation of the biosynthetic mechanism and the biological function of gramicidin S.

Draft Genome Sequence of Thermophilic Geobacillus sp. Strain Sah69, Isolated from Saharan Soil, Southeast Algeria

Bezuidt, Oliver K. I.; Makhalanyane, Thulani P.; Gomri, Mohamed A.; Kharroub, Karima; Cowan, Don A.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Geobacillus spp. are potential sources of novel enzymes, such as those involved in the degradation of recalcitrant polymers. Here, we report a Geobacillus genome that may help reveal genomic differences between this strain and publicly available representatives of the same genus from diverse niches.

Draft Genome Sequences of Edwardsiella ictaluri Strains LADL11-100 and LADL11-194 Isolated from Zebrafish Danio rerio

Wang, Rui; Tekedar, Hasan C.; Lawrence, Mark L.; Chouljenko, Vladimir N.; Kim, Joohyun; Kim, Nayong; Kousoulas, Konstantin G.; Hawke, John P.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Here, we report the draft genome sequences of Edwardsiella ictaluri strains LADL11-100 and LADL11-194, two isolates from natural outbreaks of edwardsiellosis in the zebrafish Danio rerio, as well as the sequences of the plasmids carried by the zebrafish strain of E. ictaluri.

Complete Genome Sequence of a Tomato Isolate of Parietaria Mottle Virus from Italy

Martínez, Carolina; Aramburu, José; Rubio, Luis; Galipienso, Luis
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
We report here the complete genome sequence of isolate T32 of parietaria mottle virus (PMoV) infecting tomato plants in Turin, Italy, obtained by Sanger sequencing. T32 shares 90.48 to 96.69% nucleotide identity with other two PoMV isolates, CR8 and Pe1, respectively, whose complete genome sequences are available.

Draft Genome Sequence of Streptomyces mutabilis TRM45540, Isolated from a Hypersaline Soil Sample

Luo, Xiaoxia; Wan, Chuanxing; Zhang, Lili
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
We report here the draft genome sequence of Streptomyces mutabilis TRM45540, a strain isolated from a soil sample from Xinjiang, China. Analysis of the genome using the bioinformatics tool antiSMASH showed the presence of many unique natural-product biosynthetic pathways.

Complete Genome Sequence of Xanthomonas campestris pv. campestris Strain 17 from Taiwan

Liu, Yi-Chung; Wang, Shiuh-Chaun; Yu, Yu-Jen; Fung, Kit-Man; Yang, Ming-Te; Tseng, Yi-Hsiung; Tsai, Shih-Feng; Sun, H. Sunny; Lyu, Ping-Chiang; Chou, Shan-Ho
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Xanthomonas campestris pv. campestris 17 is a Gram-negative bacterium that is phytopathogenic to cruciferous plants in Taiwan. The 4,994,426-bp-long genome consists of 24 contigs with 4,050 protein-coding genes, 1 noncoding RNA (ncRNA) gene, 6 rRNA genes, and 55 tRNA genes.

Draft Genome Sequence of Pedobacter sp. Strain Hv1, an Isolate from Medicinal Leech Mucosal Castings

Ott, Brittany M.; Beka, Lidia; Graf, Joerg; Rio, Rita V. M.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
The Pedobacter sp. Hv1 strain was isolated from the medicinal leech, Hirudo verbana, mucosal castings. These mucosal sheds have been demonstrated to play a role in horizontal symbiont transmission. Here, we report the draft 4.9 Mbp genome sequence of Pedobacter sp. strain Hv1.

Draft Genome Sequence of CO33, a Coffee-Infecting Isolate of Xylella fastidiosa

Giampetruzzi, Annalisa; Loconsole, Giuliana; Boscia, Donato; Calzolari, Alessandra; Chiumenti, Michela; Martelli, Giovanni P.; Saldarelli, Pasquale; Almeida, Rodrigo P. P.; Saponari, Maria
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
The draft genome sequence of Xylella fastidiosa CO33 isolate, retrieved from symptomatic leaves of coffee plant intercepted in northern Italy, is reported. The CO33 genome size is 2,681,926 bp with a GC content of 51.7%.

Genome Sequences of Oblitimonas alkaliphila gen. nov. sp. nov. (Proposed), a Novel Bacterium of the Pseudomonadaceae Family

Lauer, Ana C.; Nicholson, Ainsley C.; Humrighouse, Ben W.; Emery, Brian; Drobish, Adam; Juieng, Phalasy; Loparev, Vladimir; McQuiston, John R.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Results obtained through 16S rRNA gene sequencing and phenotypic testing of eight related, but unidentified, isolates located in a historical collection at the Centers for Disease Control and Prevention suggested that these isolates belong to a novel genera of bacteria. The genomes of the bacteria, to be named Oblitimonas alkaphilia gen. nov. sp. nov., were sequenced using Illumina technology. Closed genomes were produced for all eight isolates.

Draft Genome of the Arthrobacter sp. Strain Edens01

Couger, M. B.; Hanafy, Radwa A.; Edens, Curtis; Budd, Connie; French, Donald P.; Hoff, Wouter D.; Elshahed, Mostafa S.; Youssef, Noha H.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
We report the draft genome sequence of Arthrobacter sp. strain Edens01, isolated from a leaf surface of a Rosa hybrid plant as part of the Howard Hughes Medical Institute-funded Student Initiated Microbial Discovery (SIMD) project. The genome has a total size of 3,639,179 bp and contig N50 of 454,897 bp.

Draft Genome Sequences of Six Pseudoalteromonas Strains, P1-7a, P1-9, P1-13-1a, P1-16-1b, P1-25, and P1-26, Which Induce Larval Settlement and Metamorphosis in Hydractinia echinata

Klassen, Jonathan L.; Wolf, Thomas; Rischer, Maja; Guo, Huijuan; Shelest, Ekaterina; Clardy, Jon; Beemelmanns, Christine
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
To gain a broader understanding of the importance of a surface-associated lifestyle and morphogenic capability, we have assembled and annotated the genome sequences of Pseudoalteromonas strains P1-7a, P1-9, P1-13-1a, P1-16-1b, P1-25, and P1-26, isolated from Hydractinia echinata. These genomes will allow detailed studies on bacterial factors mediating interkingdom communication.

Genome Sequence of Acinetobacter baumannii Strain D36, an Antibiotic-Resistant Isolate from Lineage 2 of Global Clone 1

Hamidian, Mohammad; Hawkey, Jane; Holt, Kathryn E.; Hall, Ruth M.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Multiply antibiotic-resistant Acinetobacter baumannii isolate D36 was recovered in Australia in 2008 and belongs to a distinct lineage of global clone 1 (GC1). Here, we present the complete 4.13 Mbp genome sequence (chromosome plus 4 plasmids), generated via long read sequencing (PacBio).

Next-Generation Whole-Genome Sequencing of Eight Strains of Bacillus cereus, Isolated from Food

Krawczyk, Antonina O.; de Jong, Anne; Eijlander, Robyn T.; Berendsen, Erwin M.; Holsappel, Siger; Wells-Bennik, Marjon H. J.; Kuipers, Oscar P.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Bacillus cereus can contaminate food and cause emetic and diarrheal foodborne illness. Here, we report whole-genome sequences of eight strains of B. cereus, isolated from different food sources.