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Oral health profile in patients infected with HTLV-1: Clinical findings, proviral load, and molecular analysis from HTLV-1 in saliva

Lins, Liliane; Carvalho, Victor Jose Uchoa de; Rego, Filipe Ferreira de Almeida; Azevedo, Rochele; Kashima, Simone; Olavarria Gallazi, Viviana Nilla; Xavier, Marcia Tosta; Galvao-Castro, Bernardo; Junior Alcantara, Luiz Carlos
Fonte: WILEY-BLACKWELL; HOBOKEN Publicador: WILEY-BLACKWELL; HOBOKEN
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.99%
Human T-lymphotropic virus type 1 (HTLV-1) is associated with adult T-cell leukemia (ATL) and HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) and has also been implicated in several disorders, including periodontal disease. The proviral load is an important biological marker for understanding HTLV-1 pathogenesis and elucidating whether or not the virus is related to the clinical manifestation of the disease. This study describes the oral health profile of HTLV-1 carriers and HAM/TSP patients in order to investigate the association between the proviral load in saliva and the severity of the periodontal disease and to examine virus intra-host variations from peripheral blood mononuclear cells and saliva cells. It is a cross-sectional analytical study of 90 individuals carried out from November 2006 to May 2008. Of the patients, 60 were HTLV-1 positive and 30 were negative. Individuals from the HTLV-1 positive and negative groups had similar mean age and social-economic status. Data were analyzed using two available statistical software packages, STATA 8.0 and SPSS 11.0 to conduct frequency analysis. Differences of P?<?0.05 were considered statistically significant. HTLV-1 patients had poorer oral health status when compared to seronegative individuals. A weak positive correlation between blood and saliva proviral loads was observed. The mean values of proviral load in blood and saliva in patients with HAM/TSP was greater than those in HTLV-1 carriers. The HTLV-1 molecular analysis from PBMC and saliva specimens suggests that HTLV-1 in saliva is due to lymphocyte infiltration from peripheral blood. A direct relationship between the proviral load in saliva and oral manifestations was observed. J. Med. Virol. 84:1428-1436...

Saliva and dental erosion

Buzalaf, Marília Afonso Rabelo; Hannas, Angélicas Reis; Kato, Melissa Thiemi
Fonte: Faculdade De Odontologia De Bauru - USP Publicador: Faculdade De Odontologia De Bauru - USP
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.99%
Dental erosion is a multifactorial condition. The consideration of chemical, biological and behavioral factors is fundamental for its prevention and therapy. Among the biological factors, saliva is one of the most important parameters in the protection against erosive wear. Objective: This review discusses the role of salivary factors on the development of dental erosion. Material and Methods: A search was undertaken on MeDLINe website for papers from 1969 to 2010. The keywords used in the research were "saliva", "acquired pellicle", "salivary flow", "salivary buffering capacity" and "dental erosion". Inclusion of studies, data extraction and quality assessment were undertaken independently and in duplicate by two members of the review team. Disagreements were solved by discussion and consensus or by a third party. Results: Several characteristics and properties of saliva play an important role in dental erosion. Salivary clearance gradually eliminates the acids through swallowing and saliva presents buffering capacity causing neutralization and buffering of dietary acids. Salivary flow allows dilution of the acids. In addition, saliva is supersaturated with respect to tooth mineral, providing calcium, phosphate and fluoride necessary for remineralization after an erosive challenge. Furthermore...

"Estudo de alguns parâmetros salivares em indivíduos com síndrome de DOWN" ; Study measurement the flow rate concenntration whole saliva of individuals with Down syndrome.

Siqueira Junior, Walter Luiz
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 20/01/2005 Português
Relevância na Pesquisa
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O objetivo deste estudo foi mensurar o fluxo salivar, pH, capacidade tampão, concentrações de proteína total e ácido siálico, atividades das enzimas amilase e peroxidase e concentração dos íons sódio, potássio, cálcio, fósforo, zinco e magnésio em saliva total de indivíduos síndrome de Down com idade entre 1 e 25 anos. Nos indivíduos com idade entre 1 e 5 anos a saliva total foi coletada através de uma leve sucção, enquanto que nos outros indivíduos com idade entre 6 e 10, 11 e 15, 15 e 20, 21 e 25 a saliva total foi coletada com estimulação mecânica através da mastigação de parafilm, durante 10 minutos. O pH e a capacidade tampão foramdeterminadas usando um pHmetro digital. A capacidade tampão foi mensurada através de titulação com HCl a 0,01N. A concentração de eletrólitos foi determinada através de um espectrofotômetro de emissão atômica com fonte de excitação de argônio induzido. A proteína total foi mensurada através do reagente de Folin. A atividade da amilase foi mensurada através da produção de maltose e a atividade da peroxidase foi mensurada através da utilização de orto-dianisidina. Para a analise estatística os dados foram apresentados em media ± desvio padrão. Foi utilizado o teste “t” de Student para determinar as diferenças entre as medias dos indivíduos síndrome de Down e o grupo controle. Nenhuma diferença significante foi observada na concentração de ácido siálico...

Comparação dos níveis de histatinas da saliva da parótida entre sujeitos com doença periodontal e sujeitos sem história prévia de doença periodontal; Comparison of histatin levels between periodontally healthy and diseased subjects

Safioti, Luciana Moreira Lima
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 01/08/2005 Português
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36.96%
As histatinas são uma família de peptídeos presentes nas secreções salivares humanas tendo como membros mais importantes da família as histatinas 1, 3 e 5. Diversos trabalhos demonstraram atividade inibitória das histatinas sobre produtos bacterianos e derivados do hospedeiro envolvidos na doença periodontal, sugerindo uma função importante de defesa das histatinas na cavidade bucal na presença de doença periodontal. Este estudo teve por objetivo comparar as quantidades de histatinas 1, 3 e 5 e o total de histatinas presentes na saliva da parótida entre sujeitos com doença periodontal e sujeitos sem história prévia de doença periodontal. Participaram do estudo 20 sujeitos da pesquisa com doença periodontal e 20 sujeitos sem história prévia de doença periodontal. As coletas da secreção da parótida foram realizadas sob condições de estimulação gustativa com balas ácidas com auxílio de um coletor de saliva Carlson-Crittenden posicionado sobre o ducto de Stenson e conectado a um cilindro de borracha resfriado em gelo. A quantificação de histatinas presentes nas secreções salivares foi realizada pelo procedimento de precipitação por zinco seguido por quantificação utilizando-se cromatografia líquida de alta pressão de fase reversa. Não foram encontradas diferenças significativas nas quantidades de histatinas na saliva da parótida entre sujeitos com doença periodontal e sujeitos sem história prévia de doença periodontal...

Infecção experimental de camundongos C57BL/6 por L.(L.) amazonensis na presença de saliva de Lu. longipalpis: estudo da relação parasito-hospedeiro com ênfase a parâmetros da imunidade; Experimental infection in C57BL/6 mice by L. (L.) amazonensis in the presence of Lu. longipalpis saliva: study of parasite host interaction with emphasis to the immunity parameters

Pernichelli, Tadeu
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 08/04/2009 Português
Relevância na Pesquisa
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Nós investigamos os efeitos da saliva de Lutzomyia longipalpis capturados no campo e colonizados em laboratório, na evolução da lesão e imunomodulação da infecção por Leishmania (Leishmania) amazonensis, uma espécie que é endêmica na América do Sul, onde causa Leishmaniose Cutânea, Leishmaniose Cutânea Disseminada Bordeline e Leishmaniose Cutânea Difusa Anérgica, com conseqüências graves aos pacientes. Com o intuito de comparar o efeito dos dois tipos de extrato de glândula salivar, camundongos C57BL/6 foram inoculados subcutaneamente no coxim plantar das patas traseiras e nas orelhas com 106 formas promastigotas de Leishmania (L.) amazonensis na presença de extrato de glândula salivar de vetores de captura e de colônia. O tamanho da lesão foi significantemente menor nos camundongos infectados com extrato de glândula salivar de vetores capturados, o que também determinou uma infiltração menos proeminente de macrófagos nas lesões e uma resposta Th2 mais branda quando comparada com aqueles inoculados com extrato de glândula salivar de vetores colonizados. Recentemente, foi mostradas diferenças nos compostos protéicos das glândulas salivares que poderiam parcialmente justificar a expressão da lesão. Portanto...

Desenvolvimento de metodologia analítica para a investigação de anfetaminas em amostras de saliva, empregando cromatografia em fase gasosa acoplada a espectrometria de massas; Development of analytical methodology for the investigation of amphetamines and derivatives in oral fluid samples using gas chromatography coupled with mass spectrometry

Bazzarella, Rafael Barcellos
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 30/03/2010 Português
Relevância na Pesquisa
36.99%
O uso de drogas estimulantes por motoristas é reconhecidamente responsável por um aumento significativo na quantidade de acidentes nas estradas e rodovias. A própria exigência do trabalho muitas vezes acaba direcionando-os para a busca de algo que lhes proporcione maior estado de vigília e atenção nas estradas. Com base na importante correlação entre a profissão e o uso de substâncias anfetamínicas, foi proposta uma metodologia analítica para a determinação de anfetamina, metanfetamina, 3,4 metilenodioxianfetamina, 3,4 metilenodioximetanfetamina e 3,4 metilenodioxietilanfetamina em amostras de saliva através de técnica de extração líquido-líquido e análise por cromatografia em fase gasosa acoplada a espectrometria de massas (GC-MS). Para o desenvolvimento do método foi utilizado 1 mL de saliva, extração líquido-líquido com o solvente acetato de etila, derivatização com anidrido heptafluorobutírico (HFBA) e detecção com GC-MS. A metodologia foi validada e demonstrou linearidade de 10 a 400 ng/mL de saliva para todos os compostos. Os limites de detecção estabelecidos se encontraram entre 2,5 ng/mL e 7,5 ng/mL, enquanto os de quantificação foram de 10 ng/mL para todos os compostos. A exatidão apresentou valores entre 93...

Presença de genes de resistência a agentes antimicrobianos em saliva, biofilme supragengival e canais radiculares infectados; Presence of antimicrobial resistance genes in saliva, supragingival biofilm and infected root canals

Moraes, Ludmila Coutinho
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Dissertação Formato: application/pdf
Português
Relevância na Pesquisa
36.99%
Embora diferentes estudos indiquem que há um aumento da resistência dos microrganismos aos agentes antimicrobianos prescritos, pouco se sabe a respeito da sua distribuição na cavidade oral. A presente dissertação está dividida em dois capítulos, representados por dois diferentes manuscritos. No manuscrito I, uma revisão sistemática foi realizada e teve como objetivo determinar quais genes de resistência a antimicrobianos foram pesquisados em saliva (S), no biofilme supragengival (SB) e canal radicular (RC). Os termos foram usados em várias combinações nas seguintes bases de dados eletrônicas (MEDLINE, EMBASE, ISI, SCOPUS e OPENGREY). Após seleção dos títulos e análise dos resumos, o texto integral de cada estudo foi obtido. Foram estabelecidos critérios de inclusão e exclusão. Dados epidemiológicos, características metodológicas e os resultados foram obtidos a partir dos estudos. Devido à falta de padronização da metodologia entre os trabalhos, não foi possível realizar uma meta-análise. Realizou-se análise descritiva dos dados. Um total de 151 títulos foram identificados para análise preliminar. Quarenta e nove resumos foram selecionados e 22 textos completos foram obtidos. Sete artigos contemplavam aos critérios de inclusão (S = 2; SB = 0 ...

Factors Associated with Fluoride Concentrations in Whole and Parotid Ductal Saliva

Fukushima, R.; Pessan, J. P.; Sampaio, F. C.; Buzalaf, M. A. R.
Fonte: Karger Publicador: Karger
Tipo: Artigo de Revista Científica Formato: 568-573
Português
Relevância na Pesquisa
36.99%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 02/07875-8; Processo FAPESP: 03/03660-0; Processo FAPESP: 03/03662-2; Processo FAPESP: 04/15417-5; Background/Aims: There are still uncertainties regarding the use of whole and parotid ductal saliva as indicators of chronic exposure to fluoride. This study evaluated the effect of water fluoride concentration, age, gender, geographical area and localization (urban/rural) on fluoride concentrations in whole and ductal saliva. Methods: Subjects (n = 300) aged 3-7, 14-20, 30-40 and 50-60 years, from five communities (A-E) with different fluoride concentrations in the drinking water, participated in the study. Two samples of drinking water and parotid and whole saliva were collected for each subject and were analyzed for fluoride using appropriate electrode techniques. Results: Mean water F concentrations (+/- SE, mg/l, n = 60) were 0.09 +/- 0.01, 0.15 +/- 0.01, 0.66 +/- 0.01, 0.72 +/- 0.02, and 1.68 +/- 0.08 for A-E, respectively. Mean F concentrations (+/- SE, mg/l, n = 15) ranged between 0.014 +/- 0.002 (A, 3-7 years) and 0.297 +/- 0.057 (D, 14-20 years) for whole saliva and 0.009 +/- 0.001 (C, 30-40 years) and 0.284 +/- 0.038 (E, 50-60 years) for parotid saliva. Results of multivariate linear regression analysis showed that geographical area and water fluoride concentration exerted the strongest influence in whole and ductal saliva F concentrations...

Cortisol in saliva and plasma of cattle after ACTH administration and milking

Negrao, J. A.; Porcionato, M. A.; de Passille, A. M.; Rushen, J.
Fonte: Amer Dairy Science Assoc-adsa Publicador: Amer Dairy Science Assoc-adsa
Tipo: Artigo de Revista Científica Formato: 1713-1718
Português
Relevância na Pesquisa
36.99%
Interest in the measurement of salivary cortisol has increased recently because saliva can be easily collected before and after an imposed stress. This study evaluated the relationship between plasma and salivary concentrations of cortisol following ACTH administration in calves ( experiment 1) and machine milking of adult cows ( experiment 2). A catheter was inserted into the jugular vein of all animals 72 h before the beginning of experiments. Blood and saliva samples were collected before and after ACTH administration (0.6 IU/kg BW) in calves or before and after machine milking of cows. Using a cotton swab, each saliva sample was taken immediately following the blood sample. In general, cortisol profiles were similar in plasma and saliva and correlated in both experiments; however, plasma concentrations were significantly higher than salivary concentrations. In addition, the differences between cortisol concentrations measured in saliva and plasma within each experiment varied substantially between animals and samples. Furthermore, in experiment 2, nearly 10% of salivary samples were below limits of detection. The sharp peaks in cortisol after ACTH administration in both the plasma and saliva were reflected adrenal stimulation. In addition...

Avaliação comparativa in vivo da eficácia do óleo de melaleuca, clorexidina e listerine sobre streptococcus mutans e microrganismos totais na saliva

Nogueira, Marianne Nicole Marques; Correia, Marilia Ferreira; Fontana, Amanda; Bedran, Telma Blanca Lombard; Spolidorio, Denise Madalena Palomari
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 343-349
Português
Relevância na Pesquisa
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Objective: To compare the efficacy of the mouthwashes 0.12% chlorhexidine, Listerine, and 0.5% and 2% Melaleuca Alternifolia oil against the salivary levels of Streptococcus mutans and total microorganisms. Methods: This study was double-blind controlled and paired clinical assay. Twenty-six volunteers aged 21 to 35 years old were enrolled. At baseline, 1 mL of unstimulated saliva was collected from each subject, 1 and 15 min after mouthrinsing with the following solutions: sterile distilled water, 0.12% chlorhexidine digluconate, Listerine (©Johnson & Johnson do Brasil), 0.5% and 2% concentrations of Melaleuca Alternifolia (Sigma-Aldrich). The volunteers used all the evaluated mouthrinses with a 15-day interval between the solutions. Immediately after rinsing, saliva was collected and serial dilutions were performed, followed by plating in blood agar culture medium for growth of total microorganisms and SB-20 (Sucrose-Bacitracin agar) for growth of S. mutans, and incubation at 37 °C for 48 h in microaerophilia. After incubation, the number of colonies was counted and expressed as colony forming units (UFC/mL). Results: Chlorhexidine showed antimicrobial action by reducing total microorganisms and S. mutans, while the action of 0.5% Melaleuca Alternifolia was similar to that of distilled water. Listerine and 2% Melaleuca Alternifolia oil reduced total microbial counts by 11% and 9% respectively...

Análise proteômica revela que a saliva de Dipetalogaster maxima é rica em lipocalinas e possui apirase da família 5’ nucleotidase

Santiago, Paula Beatriz
Fonte: Universidade de Brasília Publicador: Universidade de Brasília
Tipo: Dissertação
Português
Relevância na Pesquisa
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Dissertação (mestrado)—Universidade de Brasília, Faculdade de Medicina, 2011.; A pesquisa em doença de Chagas acumula bons resultados no que se refere à transmissão do Trypanosoma cruzi. Contudo, fatores devem ser considerados: a ocupação humana de áreas naturalmente habitadas por esses insetos e a presença constante do T. cruzi em populações animais aumentam o risco de novas transmissões ao homem. Esse cenário é similar ao encontrado na região sul da Baixa Califórnia, México, onde a invasão do ambiente do triatomíneo da espécie Dipetalogaster maxima pelo homem mostrou a versatilidade alimentar do vetor, que além do sangue de lagartos, passou a se alimentar do sangue humano. O fluxo sanguíneo deve ser constante para o sucesso do repasto e para contrapor a hemostasia do hospedeiro os triatomíneos hematófagos apresentam uma fonte complexa de proteínas em sua saliva, como vasodilatadores, anti-agregadores plaquetários e anti-coagulantes. O trabalho aqui realizado demonstrou que a saliva do triatomíneo D. maxima contém proteínas anti-hemostáticas já descritas na saliva de outros triatomíneos como Rhodnius prolixus, Triatoma infestans e Triatoma pallidipennis. A análise proteômica revelou as três maiores famílias de proteínas salivares encontradas em outros triatomíneos: lipocalina...

Nitric oxide levels are not changed in saliva of patients infected with hepatitis C virus

Tavares,Fernando N.; Gonçalves,Patricia L.; Porto,Simone A.C.; Pereira,Fausto E.L.; Ribeiro-Rodrigues,Rodrigo
Fonte: Sociedade Brasileira de Medicina Tropical - SBMT Publicador: Sociedade Brasileira de Medicina Tropical - SBMT
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2005 Português
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36.99%
The aim of this investigation was to determine nitric oxide metabolite levels in saliva samples from hepatitis C virus-positive patients in an attempt to test the hypothesis if increased levels of nitric oxide metabolites correlates with the presence of HCV-RNA in saliva. Saliva of 39 HCV-positive patients and 13 HCV-negative patients, without clinical or laboratorial evidence of liver disease were tested for nitric oxide metabolites. HCV-RNA was detected in serum and saliva by a RT-PCR method and nitric oxide level was determined by evaluation of its stable degradation products, nitrate and nitrite. No differences were found between the concentration of nitrite in saliva from HCV patients and controls, in despite of the presence or not of HCV RNA in saliva. Patients with HCV and cirrhosis had higher concentrations of nitrite but not significantly different from the control group or the groups of anti-HCV patients without cirrhosis. Increased levels of nitrite were not detected in anti-HCV positive patients, an indirect indication that chronic sialoadenitis are infrequent in these patients or occurs with low intensity not sufficient to increase nitric oxide metabolite levels in saliva.

Saliva and dental erosion

Buzalaf,Marília Afonso Rabelo; Hannas,Angélicas Reis; Kato,Melissa Thiemi
Fonte: Faculdade De Odontologia De Bauru - USP Publicador: Faculdade De Odontologia De Bauru - USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2012 Português
Relevância na Pesquisa
36.99%
Dental erosion is a multifactorial condition. The consideration of chemical, biological and behavioral factors is fundamental for its prevention and therapy. Among the biological factors, saliva is one of the most important parameters in the protection against erosive wear. Objective: This review discusses the role of salivary factors on the development of dental erosion. Material and Methods: A search was undertaken on MeDLINe website for papers from 1969 to 2010. The keywords used in the research were "saliva", "acquired pellicle", "salivary flow", "salivary buffering capacity" and "dental erosion". Inclusion of studies, data extraction and quality assessment were undertaken independently and in duplicate by two members of the review team. Disagreements were solved by discussion and consensus or by a third party. Results: Several characteristics and properties of saliva play an important role in dental erosion. Salivary clearance gradually eliminates the acids through swallowing and saliva presents buffering capacity causing neutralization and buffering of dietary acids. Salivary flow allows dilution of the acids. In addition, saliva is supersaturated with respect to tooth mineral, providing calcium, phosphate and fluoride necessary for remineralization after an erosive challenge. Furthermore...

EFFECT OF GUM CHEWING ON AIR SWALLOWING, SALIVA SWALLOWING AND BELCHING

SILVA,Ana Cristina Viana da; APRILE,Lilian Rose Otoboni; DANTAS,Roberto Oliveira
Fonte: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE ; Colégio Brasileiro de Cirurgia Digestiva - CBCD ; Sociedade Brasileira de Motilidade Digestiva - SBMD ; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED Publicador: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE ; Colégio Brasileiro de Cirurgia Digestiva - CBCD ; Sociedade Brasileira de Motilidade Digestiva - SBMD ; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2015 Português
Relevância na Pesquisa
36.99%
BackgroundEructation is a physiologic event which allows gastric venting of swallowed air and most of the time is not perceived as a symptom. This is called gastric belching. Supragastric belching occurs when swallowed air does not reach the stomach and returns by mouth a short time after swallowing. This situation may cause discomfort, life limitations and problems in daily life.ObjectiveOur objective in this investigation was to evaluate if gum chewing increases the frequency of gastric and/or supragastric belches.MethodsEsophageal transit of liquid and gas was evaluated by impedance measurement in 16 patients with complaint of troublesome belching and in 15 controls. The Rome III criteria were used in the diagnosis of troublesome belching. The esophageal transit of liquid and gas was measured at 5 cm, 10 cm, 15 cm and 20 cm from the lower esophageal sphincter. The subjects were evaluated for 1 hour which was divided into three 20-minute periods: (1) while sitting for a 20-minute base period; (2) after the ingestion of yogurt (200 mL, 190 kcal), in which the subjects were evaluated while chewing or not chewing gum; (3) final 20-minute period in which the subjects then inverted the task of chewing or not chewing gum. In gastric belch...

Analysis of saliva by Fourier transform infrared spectroscopy for diagnosis of physiological stress in athletes

Caetano Júnior,Paulo Cesar; Strixino,Juliana Ferreira; Raniero,Leandro
Fonte: Sociedade Brasileira de Engenharia Biomédica Publicador: Sociedade Brasileira de Engenharia Biomédica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2015 Português
Relevância na Pesquisa
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Introduction Saliva is the most promising biofluid to monitor the physiological state of athletes, because this method is not invasive and has low contamination risks. The characterization of saliva by Fourier transform infrared spectroscopy (FT-IR) has been studied as an alternative technique to the standard clinical analysis. However, methodological procedures for saliva analysis are not completely clear, especially in terms of influence of storage conditions and sample preparations for infrared analysis. Thawed saliva includes a precipitate, which may influence the infrared spectral analysis. Thus, the purpose of this study was to show the spectral differences of the precipitate, supernatant, and a combo, as well as the best way to classify the physiological state of the athletes by FT-IR. Methods The saliva collection was performed before, immediately after, and two hours after a handball match. After the storage of samples at –20 ○C, it was possible to identify two phases (precipitate and supernatant) and to determine the biochemical differences between the spectra of each phase, which were distinctly analyzed by the second derivative and deconvolution bands. Results The precipitate and supernatant results showed characteristic bands...

Saliva and dental erosion

Buzalaf, Marília Afonso Rabelo; Hannas, Angélicas Reis; Kato, Melissa Thiemi
Fonte: Universidade de São Paulo. Faculdade de Odontologia de Bauru Publicador: Universidade de São Paulo. Faculdade de Odontologia de Bauru
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; Formato: application/pdf
Publicado em 01/10/2012 Português
Relevância na Pesquisa
36.99%
Dental erosion is a multifactorial condition. The consideration of chemical, biological and behavioral factors is fundamental for its prevention and therapy. Among the biological factors, saliva is one of the most important parameters in the protection against erosive wear. Objective: This review discusses the role of salivary factors on the development of dental erosion. Material and Methods: A search was undertaken on MeDLINe website for papers from 1969 to 2010. The keywords used in the research were "saliva", "acquired pellicle", "salivary flow", "salivary buffering capacity" and "dental erosion". Inclusion of studies, data extraction and quality assessment were undertaken independently and in duplicate by two members of the review team. Disagreements were solved by discussion and consensus or by a third party. Results: Several characteristics and properties of saliva play an important role in dental erosion. Salivary clearance gradually eliminates the acids through swallowing and saliva presents buffering capacity causing neutralization and buffering of dietary acids. Salivary flow allows dilution of the acids. In addition, saliva is supersaturated with respect to tooth mineral, providing calcium, phosphate and fluoride necessary for remineralization after an erosive challenge. Furthermore...

Cocaine and benzoylecgonine in saliva, serum, and urine

Schramm, W.; Craig, Paul; Smith, R.; Berger, G.
Fonte: American Association for Clinical Chemistry: Clinical Chemistry Publicador: American Association for Clinical Chemistry: Clinical Chemistry
Tipo: Abstract Formato: 35680 bytes; application/pdf
Português
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We studied the feasibility of using saliva to detect cocaine and benzoylecgonine. Saliva was collected as an ultrafiltrate directly in the mouth with an osmotic device. We analyzed by immunoassay matched samples of urine, blood, and salivary ultrafiltrate from 69 patients who had used cocaine within 24 h of sample collection. Cocaine concentrations were 4.9 times higher in saliva than in serum; benzoylecgonine concentrations were 2.5 times higher in serum. Seven urine and two serum samples had undetectable concentrations of cocaine, but all 69 saliva samples were positive for the drug. For benzoylecgonine detection, all urine samples were positive and three serum and one saliva sample were negative. We also analyzed 43 samples of saliva by gas chromatography/mass spectrometry: all were positive for benzoylecgonine, whereas 40 were positive for cocaine. We conclude that simultaneous measurement of cocaine and benzoylecgonine in saliva is suitable in screening for recent cocaine use.; Article may be found at: http://www.clinchem.org/cgi/content/abstract/39/3/481; These investigations were supported by a grant from the National Institute on Drug Abuse, R43 DA05982.

Diagnóstico da raiva canina: I. comparação entre amostras de saliva e de encéfalo; Diagnosis of canine rabies: I. comparison of saliva and encephalon samples

Côrtes, Valdson de Angelis; Paim, Gil Vianna; Oliveira, Maria Cecília Gibrail de
Fonte: Universidade de São Paulo. Faculdade de Saúde Pública Publicador: Universidade de São Paulo. Faculdade de Saúde Pública
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/12/1979 Português
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Foram examinadas mediante as técnicas de inoculação em camundongos e de imunofluorescência, 30 amostras de saliva e 30 amostras de encéfalo, colhidas de 30 cães raivosos. Todas as amostras, tanto de saliva como de encéfalo, apresentaram resultados positivos frente às duas técnicas usadas, demonstrando uma perfeita correlação entre os métodos e os espécimens utilizados. Foi ressaltada a importância da saliva para o estabelecimento de um diagnóstico mais precoce da raiva.; Thirty saliva samples and 30 encephalon samples, collected from an equal number of rabid dogs, were examined by both the mouse inoculation and immunoflluorescent techniques. The results for each sample were positive with both techniques, showing perfect correlation in the methods and specimens used. The importance of saliva for early diagnosis was stressed.

; Shear bond strength of adhesive systems to saliva-contaminated dentin

Sálvio, Luciana Andrea; Sinhoreti, Mário Alexandre Coelho; Correr Sobrinho, Lourenço; Consani, Simonides; Konno, Alysson Noriyuki Kajishima
Fonte: UNICAMP/FOP Publicador: UNICAMP/FOP
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ;
Publicado em 13/11/2015 Português
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; This study evaluated the shear bond strength of two adhesive systems: Scotchbond Multi-Purpose (SBMP) and Clearfil Liner Bond 2V (CLB2V) on different dentin surface conditions. Bovine incisors were collected and prepared according to the ISO’s (TR 11405) directions. The samples were divided into nine groups (n=10): 1- SBMP control group (without contamination); 2-SBMP: saliva contamination after priming; 3- SBMP: saliva contamination after bond application; 4-SBMP: saliva contamination after acid etching; 5-SBMP: as group 4 with re-etching after saliva contamination; 6-CLB2V control group (without contamination); 7-CLB2V: saliva contamination after priming; 8-CLB2V: saliva contamination after bond application; and, 9-CLB2V: saliva contamination before priming. After the storage period in water at 37o C, the samples were tested with an Instron testing machine, at crosshead speed of 0.5mm/min. The results were submitted to ANOVA (one way) and Tukey’s test at 5% significance level. For SBMP groups, the mean shear bond strengths were: 1 - 4.8 MPa, 2 - 5.0 MPa, 3 - 5.2 MPa, 4 - 6.6 MPa and 5 - 4.2 MPa. There was no statistical difference among groups tested. For CLB2V groups 6, 8, 9, the mean shear bond strengths were 8.0 MPa, 6.9 MPa and 8.8 MPa respectively and demonstrated no statistical differences. Group 7 (4.7 MPa) differed from all other groups except 8. Slight salivary contamination does not influence the shear bond strength of SBMP during any stage and slight salivary contamination influences the shear bond strength of CLB2V after priming step.

Saliva flow rate and composition in lysinuric protein intolerance

Söderling, Eva; Parto, Katriina; Simell, Olli
Fonte: UNICAMP/FOP Publicador: UNICAMP/FOP
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ;
Publicado em 15/10/2015 Português
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The aim of this study was to measure saliva flow rate and analyse microbiological, chemical and biochemical features of saliva in patients with lysinuric protein intolerance (LPI). The study comprised 20 patients with LPI (age 11 to 44 years) and 20 controls. Fourteen of the patients showed growth retardation. The LPI patients' diet consisted predominantly of fats and carbohydrates and contained small amounts of proteins. At the appointments, paraffin-stimulated saliva was collected. Buffer effect, mutans streptococci, lactobacilli and yeasts were measured using semiquantitative assays. Calcium, phosphate, ammonia, urea, protein, lysozyme, amylase and amino acids were o determined from saliva stored at -20 C. The mean saliva flow rates of the patients with LPI were lower than those of the controls (p < 0.05). Salivary pH and buffer effect did not differ significantly between the groups. Of the analysed microbiological, chemical and biochemical features of saliva, only the amylase activity was lower in the LPI patients than their controls (p < 0.05). Growth retardation associated with low saliva flow rates. No other differences in the analysed variables of the salivas between the growth-retarded and normal-sized patients were detected. Our data indicate that the LPI patients should not be particularly predisposed to caries despite their low-protein...