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Detecção do gene mecA em estafilococos coagulase negativa resistentes à oxacilina isolados da saliva de profissionais da enfermagem; Detection of mecA gene in oxacillin-resistant coagulase-negative staphylococci isolated from the saliva of nursing professionals

ROSA, Juliana de Oliveira; MOURA, Josely Pinto de; PALOS, Marinésia Aparecida Prado; GIR, Elucir; REIS, Cleômenes; KIPNIS, André; CANINI, Sílvia Rita Marin da Silva; BELISSIMO-RODRIGUES, Fernando; PIMENTA, Fabiana Cristina
Fonte: Sociedade Brasileira de Medicina Tropical - SBMT Publicador: Sociedade Brasileira de Medicina Tropical - SBMT
Tipo: Artigo de Revista Científica
Português
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Estafilococos coagulase negativa estão frequentemente associados às infecções nosocomiais e os profissionais da saúde podem ser reservatório e dissemina-los no hospital e comunidade. O objetivo desse estudo foi identificar espécies de estafilococos coagulase negativa isolados da saliva de profissionais da enfermagem, determinar o perfil de resistência e detectar o gene mecA. Foram selecionados 100 estafilococos coagulase negativa, sendo 41 identificados como Staphylococcus epidermidis, 25 Staphylococcus saprophyticus, 18 Staphylococcus haemolyticus, 8 Staphylococcus cohnii, 4 Staphylococcus lugdunenses, 3 Staphylococcus capitis, e 1 Staphylococcus Simulans. Desses, 32% apresentaram resistência à oxacilina, 84,4% à mupirocina, 32% à cefoxitina, e todos sensíveis a vancomicina. Dos estafilococos coagulase negativa resistentes à oxacilina, 93,7% desenvolveram-se no agar oxacilina (6µg/ml) e o gene mecA foi detectado em 75%. Os resultados sinalizam que maiores investimentos devem ser direcionados a identificação das espécies de estafilococos coagulase negativa nas instituições de saúde e na comunidade.; Coagulase-negative staphylococci are frequently associated with nosocomial infections, and healthcare professionals can be reservoirs and spread them in hospitals and in the community. The aim of this study was to identify species of coagulase-negative staphylococci isolated from the saliva of nursing professionals...

Saliva and tongue coating pH before and after use of mouthwashes and relationship with parameters of halitosis

TOLENTINO, Elen de Souza; CHINELLATO, Luiz Eduardo Montenegro; TARZIA, Olinda
Fonte: Faculdade De Odontologia De Bauru - USP Publicador: Faculdade De Odontologia De Bauru - USP
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.82%
OBJECTIVES: The aim of this work was to evaluate saliva and tongue coating pH in oral healthy patients with morning bad breath before and after use of different oral mouthrinses. MATERIAL AND METHODS: Saliva and tongue coating pH of 50 patients allocated in 5 groups were measured respectively by a digital pHmeter and color pH indicators, before, immediately after and 30 min after rinsing 5 different mouthrinses: cetilpiridine chloride associated with sodium chloride, triclosan, enzymatic solution, essential oil and distilled water. RESULTS: Only triclosan and essential oil increased salivary pH immediately after rising. The enzymatic solution decreased salivary and tongue coating pH immediately after rinsing. CONCLUSIONS: Salivary pH tended to be acidic while tongue coating pH tended to be alkaline, even after rising. Triclosan and essential oil mouthrinses increased salivary pH immediately after rinsing. Enzymatic solution decreased saliva and tongue coating pH immediately after rising.; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)

EIS study of Ti-23Ta alloy in artificial saliva

ROBIN, A.; MEIRELIS, J. P.
Fonte: MANEY PUBLISHING Publicador: MANEY PUBLISHING
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.82%
Although titanium and Ti-6Al-4V alloy have been widely used as dental materials, possible undesirable effects such as cytotoxic reactions and neurological disorder due to metal release led to the development of more corrosion resistant and V and Al free titanium alloys, containing Nb, Zr, Mo and Ta atoxic elements. Fluoride containing products used in the prevention of plaque formation and dental caries can affect the stability of the passive oxide films formed on the Ti alloys. In this work, the corrosion behaviour of the new Ti-23Ta alloy has been evaluated in artificial saliva of different pH and fluoride concentration using electrochemical impedance spectroscopy. Electrochemical impedance spectroscopy study showed that the oxide film formed on the alloy in artificial saliva consists of an inner compact film and an outer porous layer. The corrosion resistance of Ti-23Ta alloy which is reduced by increasing F concentration or decreasing pH is related to the resistance of the inner compact layer. The presence of fluoride and low pH of the saliva enhance the porosity of the oxide film and its dissolution.

Shear bond strength of metallic brackets : influence of saliva contamination

Retamoso, Luciana Borges; Collares, Fabrício Mezzomo; Ferreira, Eduardo Silveira; Samuel, Susana Maria Werner
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
Relevância na Pesquisa
36.88%
Objective: To evaluate the influence of saliva contamination on shear bond strength and the bond failure pattern of 3 adhesive systems (Transbond XT, AdheSE and Xeno III) on orthodontic metallic brackets bonded to human enamel. Material and Methods: Seventy-two permanent human molars were cut longitudinally in a mesiodistal direction, producing seventy-two specimens randomly divided into six groups. Each system was tested under 2 different enamel conditions: no contamination and contaminated with saliva. In T, A and X groups, the adhesive systems were applied to the enamel surface in accordance with manufacturer’s instructions. In TS, AS and XS groups, saliva was applied to enamel surface followed by adhesive system application. The samples were stored in distilled water at 37ºC for 24 h, and then tested for shear bond strength in a universal testing machine (Emic, DL 2000) running at a crosshead speed of 1 mm/min. After bond failure, the enamel surfaces were observed under an optical microscope at 40x magnification. Results: The control and contaminated groups showed no significant difference in shear bond strength for the same adhesive system. However, shear bond strength of T group (17.03±4.91) was significantly higher than that of AS (8.58±1.73) and XS (10.39±4.06) groups (p<0.05). Regarding the bond failure pattern...

Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome

Lamy, E; da Costa, G; Santos, R; Capela e Silva, F; Potes, J; Pereira, A; Coelho, AV; Sales Baptista, E
Fonte: Wiley Publicador: Wiley
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.88%
Saliva appears as a defence mechanism, against potential negative effects of tannins, in some species of animals which have to deal with these plant secondary metabolites in their regular diets. This study was carried out to investigate changes in parotid saliva protein profiles of sheep Ovis aries)and goats (Capra hircus), induced by condensed tannin ingestion. Five Merino sheep and five Serpentina goats were maintained on a quebracho tannin enriched diet for 10 days. Saliva was collected through catheters inserted on parotid ducts and salivary proteins were separated by two dimensional gel electrophoresis. Matrix assisted Laser desorption ionization – time of flight(MALDI-TOF)and liquid chromatography tandem mass spectrometry (LC-MS/MS) were used to identify the proteins whose expression levels changed after tannin consumption. Although no new proteins appeared, quebracho tannin consumption increased saliva total protein concentration and produced changes in the proteome of both species. While some proteins were similarly altered in both species parotid salivary protein profile, sheep and goats also presented species-specific differences in response to tannin consumption.

THE ONTOGENY OF SALIVA SECRETION IN INFANTS AND ESOPHAGOPROTECTION

COLLARES,Edgard Ferro; FERNANDES,Maria Inez Machado
Fonte: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE ; Colégio Brasileiro de Cirurgia Digestiva - CBCD ; Sociedade Brasileira de Motilidade Digestiva - SBMD ; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED Publicador: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE ; Colégio Brasileiro de Cirurgia Digestiva - CBCD ; Sociedade Brasileira de Motilidade Digestiva - SBMD ; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2015 Português
Relevância na Pesquisa
36.88%
Background Several studies have reported that severe reflux esophagitis is rare in infants despite the well known high occurrence of regurgitation in early infancy. There is evidence of the importance of saliva for the pre-epithelial protection of the esophageal mucosa. Results A longitudinal study conducted on healthy infants indicated that the stimulated capacity of saliva secretion (saliva output per kg of body weight) was significantly higher during their first year of age compared to older children and adults. In addition, this secretion pattern was also observed in low weight newborns during the first weeks of life and persisted in infants with severe protein-calorie malnutrition (marasmus). Conclusion The greater ability to secrete saliva is an important physiological condition that may protect the infant from acid/pepsin aggression to the esophagus during early stages of development.

Dot-ELISA-IgM in saliva for the diagnosis of human leptospirosis using polyester fabric-resin as support (Preliminary Report)

Silva,Marcos Vinicius da; Nakamura,Paulo Mutuko; Camargo,Eide Dias; Batista,Luíza; Vaz,Adelaide José; Brandão,Angela Pires; Ferreira,Antonio Walter
Fonte: Instituto de Medicina Tropical Publicador: Instituto de Medicina Tropical
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/1994 Português
Relevância na Pesquisa
36.88%
In order to improve the diagnosis of human leptospirosis, we standardized the dot-ELISA for the search of specific IgM antibodies in saliva. Saliva and serum samples were collected simultaneously from 20 patients with the icterohemorrhagic form of the disease, from 10 patients with other pathologies and from 5 negative controls. Leptospires of serovars icterohaemorrhagiae, canicola, hebdomadis, brasiliensis and cynopteri grown in EMJH medium and mixed together in equal volumes, were used as antigen at individual protein concentration of 0.2 µg/µl. In the solid phase of the test we used polyester fabric impregnated with N-methylolacrylamide resin. The antigen volume for each test was 1µl, the saliva volume was 8 µl, and the volume of peroxidase-labelled anti-human IgM conjugate was 30 µl. A visual reading was taken after development in freshly prepared chromogen solution. In contrast to the classic nitrocellulose membrane support, the fabric support is easy to obtain and to handle. Saliva can be collected directly onto the support, a fact that facilitates the method and reduces the expenses and risks related to blood processing.

Therapeutic carbamazepine (CBZ) and valproic acid (VPA) monitoring in children using saliva as a biologic fluid

Maldonado,C.; Fagiolino,Pietro; Vázquez,M.; Rey,A.; Olano,I.; Eiraldi,R.; Scavone,C.
Fonte: Liga Brasileira de Epilepsia (LBE) Publicador: Liga Brasileira de Epilepsia (LBE)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2008 Português
Relevância na Pesquisa
36.88%
OBJECTIVE: The aim of the study was to analyze retrospectively carbamazepine (CBZ) and valproic acid (VPA) salivary data collected from epileptic children during a 3-year period. METHODS: Saliva samples stimulated by citric acid were assayed by FPIA method. One hundred and three patients (aged 1-14 years) were in CBZ or VPA monotherapy or in CBZ-VPA combined therapy. RESULTS: VPA salivary levels were linearly related with daily dose, but a non-linear relationship was found for CBZ, in patients under monotherapy. VPA did not alter saliva CBZ concentration. Conversely, CBZ reduced VPA salivary levels. Non-responsive children displayed higher VPA concentrations. CBZ levels in uncontrolled patients showed non-significant difference in relation with controlled subjects even though their daily doses were higher. CONCLUSION: Citric acid stimulated saliva is reliable enough to perform therapeutic drug monitoring. Saliva drug levels in non-responsive patients would be explained according to the generalized efflux transporter overexpression hypothesis.

Influence of time, toothpaste and saliva in the retention of Streptococcus mutans and Streptococcus sanguinis on different toothbrushes

SCHMIDT,Julia Caroline; BUX,Miriam; FILIPUZZI-JENNY,Elisabeth; KULIK,Eva Maria; WALTIMO,Tuomas; WEIGER,Roland; WALTER,Clemens
Fonte: Faculdade De Odontologia De Bauru - USP Publicador: Faculdade De Odontologia De Bauru - USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2014 Português
Relevância na Pesquisa
36.88%
Objectives: The intraoral transmission of cariogenic and periodontopathogenic species seems to be facilitated by contaminated toothbrushes and other oral hygiene devices. The aim of this investigation was to analyze the in vitro retention and survival rate of Streptococcus mutans and Streptococcus sanguinis on different toothbrushes. The impacts of human saliva and antimicrobial toothpaste on these parameters were further evaluated. Material and Methods: Part I: Four toothbrushes (Colgate 360°, Curaprox CS5460 ultra soft, elmex InterX, Trisa Flexible Head3) were contaminated by S. mutans DSM 20523 or S. sanguinis DSM 20068 suspensions for three minutes. Bacteria were removed from the toothbrushes after either three minutes (T0) or 24 hours (T24) of dry storage and grown on Columbia blood agar plates for the quantification of colony-forming units (CFUs). Part II: The effects of saliva from a caries-active or a caries-inactive person and of toothpaste containing 0.12% chlorhexidine digluconate were also tested. Results: Part I: After three minutes of dry storage, approximately one percent of the bacteria were still detectable on the toothbrushes. After 24 hours, S. sanguinis exhibited a more pronounced decrease in viable cell numbers compared with S. mutans but the differences were not significant (Kruskal-Wallis test...

Detection of Helicobacter pylori in gastric biopsies, saliva and dental plaques of dyspeptic patients from Marília, São Paulo, Brazil: presence of vacA and cagA genes

Rasmussen,LT; Labio,RW de; C Neto,A; Silva,LC; Queiroz,VF; Smith,MAC; Payão,SLM
Fonte: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP Publicador: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2012 Português
Relevância na Pesquisa
36.88%
Helicobacter pylori, a gram-negative bacterium, possesses two important virulence factors: the vacuolating toxin (vacA), and the cytotoxin-associated gene product (cagA). The aim of the present study was to evaluate the presence of H. pylori in the stomach and oral cavity of humans and compare the cagA and vacA genotypes of H. pylori found in different samples (stomach, saliva and dental plaque) from the same patient. Gastric biopsies, saliva and dental plaques were obtained from 62 dyspeptic adults. DNA was extracted and evaluated for the presence of H. pylori and the alleles cagA and vacA. Persons with gastritis had a higher frequency of H. pylori -positive samples in the stomach while positive samples from gastric biopsies were significantly correlated with those from the oral cavity. There was a high H. pylori frequency in patients while the cagA gene was associated with vacA s1 alleles in gastric biopsies. Our results suggest a reservoir of the species in the oral cavity and that, in one patient, more than one H. pylori strain may exist in the saliva, dental plaque and stomach. We found a relationship between gastric infection and the bacterium in the oral cavity, with the cytotoxin genotype varying between saliva and dental plaque.

Detecção do DNA dos vírus EBV e CMV na saliva de indivíduos infectados ou não pelo HIV

Ribeiro Tenório de França, Talita; Carneiro Leão, Jair (Orientador)
Fonte: Universidade Federal de Pernambuco Publicador: Universidade Federal de Pernambuco
Tipo: Outros
Português
Relevância na Pesquisa
36.88%
Os vírus Epstein- Barr (EBV) e Citomegalovirus (CMV) apresentam ampla distribuição na população e são responsáveis pelo desenvolvimento de várias doenças. A saliva pode conter uma grande quantidade desses herpes vírus e é um veículo comum de transmissão horizontal entre indivíduos próximos. O objetivo do presente estudo foi determinar a detecção de DNA do EBV e do CMV na saliva de indivíduos infectados pelo HIV ou não e em seus familiares, avaliando papel exercido pela imunodeficiência na transmissão salivar. O grupo de estudo foi constituído por 240 indivíduos. Grupo 1: 40 indivíduos infectados pelo HIV; grupo 2: 40 indivíduos não-HIV; grupo 3: 2 parentes de cada indivíduo do grupo 1 (n=80), não parceiros-sexuais, residentes no mesmo domicílio; grupo 4: 2 parentes de cada indivíduo do grupo 2 (n=80), não parceiros-sexuais, residentes no mesmo domicílio. Foi coletada saliva não estimulada de cada participante e o DNA foi extraído usando o Kit Geneclean® II (BIO 101, La Jolla, CA, USA). A amplificação do DNA do EBV e do CMV foi realizada usando um protocolo de nested PCR. O DNA do EBV e do CMV foi detectado respectivamente em 7/40 (17,5%) e 5/40 (12,5%) indivíduos do grupo 1; 8/40 (20%) e 3/40 (7...

Avaliação da expressão da molécula HLA-G no microambiente tumoral e na saliva de pacientes com carcinoma espinocelular de cavidade oral; Evaluation of expression of molecule HLA-G in the microenvironment tumor and saliva of patients with oral squamous cell carcinoma

Gonçalves, Andréia de Souza
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Odontologia (FO); Faculdade de Odontologia - FO (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Odontologia (FO); Faculdade de Odontologia - FO (RG)
Tipo: Dissertação Formato: application/pdf
Português
Relevância na Pesquisa
36.88%
HLA-G is a molecule of the major histocompatibility complex (MHC) class I nonclassical that plays an inhibitory effect on immunocompetent cells that are fundamental in the development of an antitumoral response. In this context, the aim of this study was to evaluate the expression of HLA-G in the microenvironment of oral cavity squamous cell carcinoma (OCSCC) as well as their relationship with clinical and microscopic parameters. In addition, we aimed also to compare the salivary concentration of soluble HLA-G (sHLA-G) in patients with OCSCC with healthy individuals (control group). The techniques of immunohistochemistry and Enzyme- Linked Immunosorbent Assay (ELISA) were used. The results revealed, with the immunohistochemistry technique, that expression of HLA-G by tumor cells was significantly higher in metastatic OCSCC (n=30) (72% of samples) when compared with non-metastatic OCSCC (n=30) (28% of samples) (P=0.01 ). Moreover, patients with a lower expression of HLA-G presented a tendency to survival longer (22 months) when compared with those with a higher expression of the molecule (16 months). With reference to other clinicopathological parameters analyzed, only the presence of lymph node metastasis (P=0.01) and depth of tumor invasion (P=0.02) were significantly associated with the expression of HLA-G. By ELISA technique...

Influ??ncia da contamina????o com saliva na microinfiltra????o de restaura????es de resina composta; The effect of saliva contamination on microleakege of composite restorative

Hasan, Nihad Hasan Musa; Dam??, Josiane Luzia; Demarco, Fl??vio Fernando
Fonte: Universidade Federal de Pelotas Publicador: Universidade Federal de Pelotas
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.88%
O objetivo deste estudo foi avaliar a influ??ncia da contamina????o com saliva na microinfiltra????o marginal de restaura????es adesivas. Utilizaram-se 52 incisivos bovinos, sendo confeccionadas cavidades Classe V (vestibular/lingual), com margens em dentina e esmalte. As cavidades foram condicionadas com ??cido fosf??rico a 35% e divididas em quatro grupos. G1 ??? sem contamina????o, os demais grupos foram contaminados com saliva fresca por 15s, sendo submetidos a diferentes tratamentos: G2 ??? secas com papel absorvente; G3 ??? lavadas com ??gua; G4 ??? recondicionadas por 10s. O sistema adesivo (Single Bond/3M ESPE) foi empregado segundo recomenda????es do fabricante e as cavidades restauradas com comp??sito (Filtek Z-250/3M ESPE). Ap??s 24h as restaura????es foram polidas e termocicladas (500 ciclos/5 e 55??C/30s). Os dentes foram isolados, exceto as restaura????es e a 1mm destas, imersos em fucsina b??sica a 1% (24h) e lavados em ??gua (24h). Foram seccionados e avaliados em lupa estereosc??pica, utilizando-se escores predeterminados, por dois examinadores calibrados. Os valores obtidos foram submetidos ?? an??lise estat??stica (Kruskal-Wallis). A infiltra????o em dentina foi significantemente maior que aquela observada em esmalte (p < 0...

Quantificação e identificação de Candida sp em saliva total de pacientes HIV positivo : estudo longitudinal : Nadja Rodrigues de Melo

Nadja Rodrigues de Melo
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 08/04/1999 Português
Relevância na Pesquisa
36.88%
Desde a caracterização da AIDS no início dos anos oitenta, aumentou o número de pacientes imunossuprimidos com as mais variadas expressões clínicas associadas. Uma delas, a candidose bucal, infecção fúngica muito comum, assumiu posição de importância insuspeita até então causando assim intensificação dos estudos associados a esta importante doença. O presente estudo teve por objetivo determinar, longitudinalmente, por um ano, a contagem e identificação do gênero Candida na saliva total de pacientes portadores do HIV, correlacionando com aspectos clínicos e parâmetros laboratoriais. Foram avaliados 188 pacientes, 93 dos quais por um período mínimo de 1 ano, portadores do HIV e provenientes do Grupo de Pesquisa em OST (GPO) - UNICAMP, incluídos no protocolo de pesquisa multicêntrico randomizado, duplo cego, com inibidor de protease H/V (Protocolo MK-639), em combinação com outros antiretrovirais, fase aberta, que está sendo desenvolvido há três anos. O paciente desta pesquisa tinha 35,2 ::!: 8,2 anos, era do gênero masculino (64,9%), leucoderma (82,4%), com instrução primária (51,6%), e casado (43,6%) ou solteiro (37,2%). Eram classificados no grupo C3 (36,7%) ou 82 (28,2%) e com a categoria de exposição...

Shear bond strength of metallic brackets: influence of saliva contamination

Retamoso,Luciana Borges; Collares,Fabrício Mezzomo; Ferreira,Eduardo Silveira; Samuel,Susana Maria Werner
Fonte: Faculdade De Odontologia De Bauru - USP Publicador: Faculdade De Odontologia De Bauru - USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2009 Português
Relevância na Pesquisa
36.88%
OBJECTIVE: To evaluate the influence of saliva contamination on shear bond strength and the bond failure pattern of 3 adhesive systems (Transbond XT, AdheSE and Xeno III) on orthodontic metallic brackets bonded to human enamel. MATERIAL AND METHODS: Seventy-two permanent human molars were cut longitudinally in a mesiodistal direction, producing seventy-two specimens randomly divided into six groups. Each system was tested under 2 different enamel conditions: no contamination and contaminated with saliva. In T, A and X groups, the adhesive systems were applied to the enamel surface in accordance with manufacturer's instructions. In TS, AS and XS groups, saliva was applied to enamel surface followed by adhesive system application. The samples were stored in distilled water at 37ºC for 24 h, and then tested for shear bond strength in a universal testing machine (Emic, DL 2000) running at a crosshead speed of 1 mm/min. After bond failure, the enamel surfaces were observed under an optical microscope at 40x magnification. RESULTS: The control and contaminated groups showed no significant difference in shear bond strength for the same adhesive system. However, shear bond strength of T group (17.03±4.91) was significantly higher than that of AS (8.58±1.73) and XS (10.39±4.06) groups (p<0.05). Regarding the bond failure pattern...

Recuperación de veillonellas a partir de saliva

Gutiérrez De Ferro,M.I.; Ruiz De Valladares,R.E.; Benito De Cárdenas,I.L.
Fonte: Revista argentina de microbiología Publicador: Revista argentina de microbiología
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2005 Português
Relevância na Pesquisa
36.88%
Las veillonellas son cocos gram-negativos anaerobios asociados con salud oral. Para su aislamiento, se han reportado diferentes medios de cultivo. Las colonias de Veillonella spp. producen fluorescencia roja visible con luz ultravioleta, que desaparece en contacto con oxígeno. Esta propiedad sería útil para su identificación presuntiva rápida. Los objetivos de este trabajo fueron: 1- comparar el medio selectivo para Veillonella de Rogosa con los medios de cultivo recomendados por diferentes autores para determinar en cual de ellos se obtiene una mejor recuperación de veillonellas a partir de saliva, ya que esta muestra es generalmente utilizada para determinar la presencia y predominio de esta bacteria; 2- detectar la producción de fluorescencia en estos medios de cultivo como método rápido de identificación. Los medios de cultivo estudiados fueron: medio selectivo para Veillonella, agar Schaedler para anaerobios con vitamina K, agar tioglicolato, agar infusión cerebro corazón, agar Brucella, agar tripteína soja y agar Columbia con y sin el agregado de vancomicina y sangre lacada. La muestra ensayada fue un pool de saliva. Se hicieron recuentos de colonias de veillonellas y de microorganismos totales expresados en UFC/ml de saliva. La mayor recuperación de veillonellas en saliva se obtuvo en el medio selectivo para Veillonella con vancomicina y sangre lacada. Sólo se observó producción de fluorescencia en este medio.

Oxidative stress assessed in saliva from patients whit acute myocardial infarction: A preliminary study

Rubio,María C; González,Paula M; Ramos,Cecilia; Lewin,Pablo G; Friedman,Silvia M; Puntarulo,Susana; Nicolosi,Liliana N
Fonte: Acta Odontológica Latinoamericana Publicador: Acta Odontológica Latinoamericana
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2013 Português
Relevância na Pesquisa
36.88%
There is evidence that acute myocardial infarction (AMI) is associated with increasing production of reactive oxygen species and tissue injury. The aim of this study was to assess the presence of oxidative stress indices in saliva 24 and 48h after AMI. Materials and methods: We designed a prospective study comparing salivary levels of biomarkers of oxidative stress in patients with AMI with elevation of the ST segment in electrocardiogram versus clinically healthy subjects. Oxidative stress indices including the rate of oxidation of 2'7' dichlorohydrofluorescein diacetate (DCFHDA) and the activity of the antioxidant enzyme catalase (CAT) were evaluated in saliva from patients with AMI at 24 and 48 hours. At each sampling time, blood was drawn for serum markers of myocardial infarction. Results: This study included ten patients with acute ST-segment elevation myocardial infarction and ten clinically healthy controls. Mean age was 67.8 } 11.1 vs. 48.7 } 4.1 years (p<0.001) and gender was 60% male vs. 50% (p>0.05) for AMI vs. controls, respectively. Our results demonstrated an increase in the rate of oxidation of DCFH-DA in the myocardial infarction group as compared with controls (p=0.004), which remained unchanged at 48h. There was no difference in salivary catalase activity between controls and AMI subjects at 24h or at 48h post-diagnosis (p=0.157). The relationship between CAT48 and DCFH-DA48 was fairly significant (r=0.39; p=0.053). Conclusion: This preliminary study showed that biomarkers of oxidative stress are detectable in saliva of patients with acute myocardial infarction. Clinical Relevance: Future studies using a larger population are needed to confirm these observations and to explore the possibility of using the saliva to monitor evolving diagnosis and prognosis in acute coronary syndrome.

Aislamiento de Helicobacter pylori en mucosa gástrica, placa dental y saliva en una población de los Andes venezolanos

De Sousa,Lilibeth; Vásquez,Libia; Velasco,Judith; Parlapiano,Donatella
Fonte: Universidad del Zulia Publicador: Universidad del Zulia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2006 Português
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La infección por H. pylori es una de las más comunes en el hombre. Sin embargo, los reservorios extragástricos y las vías de transmisión permanecen en el campo de lo controversial. La cavidad oral ha sido propuesta como reservorio de H. pylori, por lo que se realizó un estudio para determinar la presencia de H. pylori en placa dental y saliva asociando estos resultados con los obtenidos en biopsia gástrica. Se estudiaron 97 pacientes dispépticos y 50 pacientes sin dispepsia a quienes se les tomó muestras de biopsia gástrica, placa dental y saliva. Las muestras de biopsia gástrica fueron evaluadas mediante métodos microbiológicos e histológicos. A las muestras de cavidad oral se les realizó cultivo y prueba de la ureasa y se incluyó un método de pretratamiento, usando urea y HCl. La prevalencia de infección por H. pylori fue de 75,5% en la totalidad de los pacientes evaluados. No se logró aislar H. pylori en saliva y placa dental de ninguno de los dos grupos estudiados, con o sin pretratamiento de la muestra. La prueba de la ureasa en placa dental fue positiva en 99,3% de los pacientes y 89,8% en saliva. No hubo diferencia estadísticamente significativa entre la frecuencia de infección por H. pylori en pacientes dispépticos y sin dispepsia. Los resultados obtenidos sugieren que la metodología empleada para la detección de H. pylori no es suficientemente sensible para la determinación del microorganismo en cavidad oral.

Las Mucinas Salivales y sus implicaciones en la reología de la saliva humana y los sustitutos salivales

Gésime,JM; Acevedo,AM; Lalaguna,F
Fonte: Facultad de Odontología -UCV Publicador: Facultad de Odontología -UCV
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2009 Português
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La literatura revisada para el presente artículo requirió el análisis de aspectos reológicos aplicables a la saliva humana y los sustitutos salivales. La saliva cuenta entre sus componentes glicoproteínas tipo mucina, las características bioquímicas de dichas glicoproteínas le confieren a la saliva sus propiedades reológicas, tales como viscosidad, lubricación y elasticidad. Las moléculas de mucina en solución presentan asociación y gelificación; la dinámica de estos procesos se ha estudiado por diversas técnicas e instrumentos. La hidratación de las mucinas es necesaria para que estas se gelifiquen, y le confieran a la saliva (o sus soluciones), viscosidad, lubricación y elasticidad, los mecanismos para que esto ocurra se apoyan en un Equilibrio de Donnan, sin embargo, en el fluido salival humano, la concentración fisiológica de mucina es tal que no permite la observación de gelificación. No obstante, a concentraciones mayores sí podría ser observada. Se han realizado estudios para el desarrollo de sustitutos salivales con compuestos de naturaleza glucídica y con mucinas extraídas de diversos tejidos animales, individualmente y en mezcla, con el objeto de aliviar la sintomatología de patologías que disminuyen el flujo salival...

Características fisicoquímicas y microbiológicas de la saliva durante y después del embarazo

Martínez-Pabón,María C.; Martínez Delgado,Cecilia M.; López-Palacio,Ana M.; Patiño-Gómez,Lina M.; Arango-Pérez,Eduin A.
Fonte: Instituto de Salud Publica, Faculdad de Medicina -Universidad Nacional de Colombia Publicador: Instituto de Salud Publica, Faculdad de Medicina -Universidad Nacional de Colombia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2014 Português
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Objetivo Identificar las variaciones producidas en algunos parámetros fisicoquímicos y microbiológicos de la saliva en un grupo de mujeres durante y después de la gestación. Materiales y Métodos Se recolectó saliva total estimulada en una cohorte de 35 mujeres embarazadas y después del parto. A cada muestra le fueron determinadas características fisicoquímicas (tasa de secreción, pH y capacidad amortiguadora) y microbiológicas (recuentos de bacterias acidogénicas). Resultados El pH y la capacidad amortiguadora de la saliva durante el embarazo fueron más bajos que en el posparto, mientras que la tasa de secreción salival fue más alta en el embarazo. Los recuentos de S. mutans y Lactobacillus spp. no presentaron cambios significativos estadísticamente, pero se observa una tendencia al aumento en los recuentos durante la gestación. Conclusión Los cambios ocurridos en la saliva de las gestantes pueden llevar a un aumento del riesgo de aparición de enfermedades bucales como caries, gingivitis y enfermedad periodontal, los cuales podrían disminuirse, mediante un adecuado diagnóstico y seguimiento odontológico, que incluya educación en salud oral a la gestante.