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Leakage of Saliva Through the Implant-Abutment Interface: In Vitro Evaluation of Three Different Implant Connections Under Unloaded and Loaded Conditions

do Nascimento, Cassio; Miani, Paola Kirsten; Pedrazzi, Vinicius; Goncalves, Reginaldo Bruno; Ribeiro, Ricardo Faria; Lapria Faria, Adriana Claudia; Macedo, Ana Paula; Albuquerque Junior, Rubens Ferreira de
Fonte: QUINTESSENCE PUBLISHING CO INC; HANOVER PARK Publicador: QUINTESSENCE PUBLISHING CO INC; HANOVER PARK
Tipo: Artigo de Revista Científica
Português
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Purpose: Bacterial leakage along the implant-abutment interface, with consequent species harboring the inner parts of two-part dental implant systems, has been reported in the literature. The aim of this in vitro study was to evaluate bacterial leakage from human saliva to the internal part of the implants along the implant-abutment interface under loaded and unloaded conditions using DNA Checkerboard. Materials and Methods: Sixty denial implants-20 each of external-hexagon, internal-hexagon, and Morse cone-connection designs-and their conical abutments were used in this study. Each group was subdivided into two groups of 10 loaded and 10 unloaded implants. The assemblies were immersed in human saliva and either (1) loaded with 500,000 cycles at 120 N (experimental group) or (2) incubated in static conditions for 7 days at 35 degrees C (unloaded control group). Results: Microorganisms were found in the internal surfaces of all types of connections. The Morse cone connection presented the lowest count of microorganisms in both the unloaded and loaded groups. Loaded implants presented with higher counts of microorganisms than unloaded implants for external- and internal-hex connections. Conclusion: Bacterial species from human saliva may penetrate along the implant-abutment interface under both unloaded and loaded conditions for all connections evaluated. Morse cone-connection implants showed the lowest counts of microorganisms for both conditions. External- and internal-hex implants showed a higher incidence of bacteria and higher bacterial counts after simulated loading. INT J ORAL MAXILLOFAC IMPLANTS 2012;27:551-560.; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) PDEE; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) - PDEE [BEX 4438/08-6]

Proteome of Rhipicephalus sanguineus tick saliva induced by the secretagogues pilocarpine and dopamine

Oliveira, C. J.; Anatriello, E.; Santos, Isabel Kinney Ferreira de Miranda; Francischetti, I. M.; Nunes, Anderson de Sá; Ferreira, B. R.; Ribeiro, J. M. C.
Fonte: Elsevier; Amsterdam Publicador: Elsevier; Amsterdam
Tipo: Artigo de Revista Científica
Português
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One dimensional gel electrophoresis was used to separate proteins from the saliva of Rhipicephalus sanguineus female ticks fed on rabbits. Gel slices were subjected to tryptic digestion and analyzed by reversed-phase HPLC followed by MS/MS analysis. The data were compared to a database of salivary proteins of the same tick and to the predicted proteins of the host. Saliva was obtained by either pilocarpine or dopamine stimulation of partially fed ticks. Electrophoretic separations of both yielded products that were identified by mass spectrometry, although the pilocarpine-derived sample was of much better quality. The majority of identified proteins were of rabbit origin, indicating the recycling of the host proteins in the tick saliva, including hemoglobin, albumin, haptoglobin, transferring, and a plasma serpin. The few proteins found that were previously associated with parasitism and blood feeding include 2 glycine-rich, cement-like proteins, 2 lipocalins, and a thyropin protease inhibitor. Among other of the 19 tick proteins identified, albeit with undefined roles, were SPARC and cyclophilin A. This catalog provides a resource that can be mined for secreted molecules that play a role in tick–host interactions.; Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP 2009/53645-3; Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP 2007/50869-2; Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP 2006/54985-4; Brazilian National Science Foundation-CNPq 559603/2009-6; Brazilian National Science Foundation-CNPq 471946/2010-9; Fundação de Amparo à Pesquisa doEstado de Minas Gerais- FAPEMIG; Intramural Research Program of the Division of Intramural Research...

Saliva de animais hematófagos : fonte de novos anticoagulantes; Saliva of hematophagous animals : source of new anticoagulants

Ciprandi, Alessandra; Horn, Fabiana; Termignoni, Carlos
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
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Esta revisão tem como objetivo apresentar os anticoagulantes e inibidores da agregação plaquetária que foram encontrados em animais hematófagos. Esses animais precisam inibir as reações hemostáticas no local onde se alimentam no hospedeiro para realizar a refeição sangüínea e também para manter o sangue fluido nos seus próprios tratos digestivos. Devido a essa necessidade, eles desenvolveram ao longo da evolução uma grande diversidade de substâncias que são injetadas no hospedeiro através da saliva e que permitiram o sucesso de seu parasitismo. Tais recursos farmacológicos podem ser utilizados como ferramentas em pesquisa da fisiologia vascular e hemostática, e têm potencial uso terapêutico em doenças cardiovasculares.; In this review, we present anticoagulants and inhibitors of platelet aggregation isolated from hematophagous animals. These animals have to inhibit, at the site of injury, the host hemostasis in order to blood-feed and maintain the blood fluid inside their digestive tract. During evolution, hematophagous animals developed a diversity of anti-homeostatic substances that are injected into the host through their saliva and that are crucial to successful parasitism. These anti-homeostatic substances could be used as tools in vascular physiology investigation and they also have potential therapeutic applications.

Correlation among mutans streptococci counts, dental caries, and IgA to Streptococcus mutans in saliva.

Koga-Ito, Cristiane Yumi; Martins, Clélia Aparecida Paiva de; Balducci, Ivan; Jorge, Antonio Olavo Cardoso
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 350-355
Português
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Two-hundred and forty individuals were studied, divided into five groups as follows: caries-free children, children with caries, children with rampant caries, young adults with and without caries. Whole stimulated saliva was collected and all individuals were investigated for DMFT/dmft according to the WHO criteria and the simplified oral hygiene index (OHI-S). Quantitative analysis of the total aerobic flora and mutans streptococci in saliva was performed. Also, the level of salivary anti-S. mutans IgA was determined by ELISA. Children with rampant caries showed the highest OHI-S value. The highest total counts of microorganisms were found in the group of children with caries. No statistically significant differences were observed for salivary flow, OHI-S and microorganism counts between the groups of young adults. No correlation between mutans streptococci counts and anti-Streptococcus mutans IgA levels was observed in the studied groups. A correlation between increased anti-Streptococcus mutans IgA levels and caries-free status was observed among young adults but not among children.

Adherence of Streptococcus mutans to uncoated and saliva-coated glass-ceramics and composites

Kantorski, Karla Zanini; Scotti, Roberto; Valandro, Luiz Felipe; Bottino, Marco Antonio; Koga-Ito, Cristiane Yumi; Jorge, Antonio Olavo
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 740-747
Português
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This study sought to investigate the surface roughness and the adherence of Streptococcus mutans (in the presence and absence of saliva) to ceramics and composites. The early dental biofilms formed in situ on the materials were illustrated, using scanning electron microscopy (SEM). Feldspathic and leucite/feldspathic ceramics and microhybrid and microfilled composites were evaluated. Human dental enamel was used as the control. Standardized specimens of the materials were produced and surface roughness was analyzed. The adhesion tests were carried out in 24-well plates and colony forming units (CFU/mL) were evaluated. Values of roughness (μm) and adherence (CFU/mL) were analyzed statistically. Of all the surfaces tested, enamel was the roughest. Leucite/feldspathic ceramics were rougher than the feldspathic ceramic, while composites were similar statistically. Enamel offered the highest level of adherence to uncoated and saliva-coated specimens, while the leucite/feldspathic ceramic demonstrated greater adherence than the feldspathic ceramic and the composites were similar statically. The rougher restorative materials increased the adherence of S, mutans on the material surfaces.

Saliva substitutes in combination with high-fluoride gel

Zandim-Barcelos, Daniela Leal; Kielbassa, Andrej M.; Sampaio, Jose Eduardo Cezar; Tschoppe, P.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 289-297
Português
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Objective This study aimed to determine whether the application of a high-fluoride gel could increase the remineralization of subsurface dentin lesions stored in saliva substitutes. Materials and methods Demineralized bovine dentin specimens were stored in mineral water (W), Glandosane (G), or modified Saliva natura (SN). Different treatments were applied twice daily: no treatment, Elmex sensitive mouth rinse (E), ProSchmelz gel (P), Duraphat toothpaste (D), ED, PD, and EPD. Differences in mineral loss were evaluated by transversal microradiography after 2 and 5 weeks. Results The treatments with E, D, and ED inhibited the mineral loss induced by G and enabled some mineral gain. ProSchmelz was not able to inhibit the demineralizing effect of G. This high-fluoride gel induced an erosive mineral loss in combination with G. The use of ProSchmelz in combination or not with other fluoride products did not increase remineralization of specimens stored in SN or W (p>0.05). ProSchmelz resulted in an erosion of the specimens stored in W and revealed a lower mineralized surface layer of specimens stored in SN. Conclusion Topical application of high-fluoride gel reduced the mineral loss induced by G but resulted in an erosion of specimens’ surface. In addition...

Caracteristicas clinicas de pacientes edentulos e presença de Candida sp. na saliva antes e 6 meses após a confecção de novas proteses totais

Fabio Ramoa Pires
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 16/07/1999 Português
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As condições clínicas intra-bucais e radiográficas dos maxilares de 107 pacientes desdentados totais foram analisadas antes da confecção de próteses totais. O fluxo salivar, a quantificação e identificação das espécies de Candida, a presença de mucosite por próteses e os valores de IgA anti Candida salivar, foram avaliados antes e seis meses após a confecção de próteses totais nos pacientes. Foram encontradas alterações clínicas e radiográficas em cerca de 30% dos pacientes, algumas das quais interferiram com o tratamento proposto e necessitaram tratamento prévio. Os valores de fluxo salivar mostraram-se normais em todos os grupos etários, mas foram menores em pacientes do sexo feminino. Níveis mais elevados de Candida na saliva foram encontrados em pacientes com fluxo salivar reduzido, em mulheres e em pacientes com higiene das próteses considerada insatisfatória. Cerca de 80% dos pacientes apresentaram Candida na saliva em ambas as consultas, sendo que a espécie C. albicans representou cerca de 73% das espécies isoladas em ambas as consultas. Cerca de 92% dos pacientes já utilizavam próteses anteriores, dos quais 54 % apresentaram mucosite por próteses na primeira consulta. Seis meses após a confecção das novas próteses...

Detecção de imunoglobulinas IgG, IgM e IgA anti-Toxoplasma gondii no soro, líquor e saliva de pacientes com síndrome da imunodeficiência adquirida e neurotoxoplasmose

Borges,Aercio Sebastião; Figueiredo,José Fernando de Castro
Fonte: Academia Brasileira de Neurologia - ABNEURO Publicador: Academia Brasileira de Neurologia - ABNEURO
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2004 Português
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Estudamos 55 pacientes com sindrome da imunodeficiência adquirida (SIDA) e neurotoxoplasmose (grupo 1); 37 pacientes com SIDA e comprometimento neurológico por outra etiologia (grupo 2) e 18 indivíduos anti-HIV negativos com manifestações neurológicas (grupo 3), pesquisando IgG, IgA e IgM anti-Toxoplasma gondii, no soro, líquor e saliva, utilizando teste ELISA, para fins diagnósticos. O valor preditivo negativo do teste para o encontro de IgG no soro foi 100% e no líquor, 92,4%. Não houve diferença entre os três grupos quanto aos anticorpos IgA neste material. Para IgA, no líquor, o teste alcançou 72,7% de especificidade (p<0,05). Na saliva, apenas o encontro de IgG mostrou correlação com o diagnóstico de neurotoxoplasmose. Enfatizamos que a ausência de anticorpos IgG anti-T. gondii no soro e líquor depõe fortemente contra o diagnóstico de neurotoxoplasmose e que imunoglobulinas IgA específicas no líquor e IgG na saliva podem representar dois marcadores auxiliares para o diagnóstico diferencial da encefalite toxoplásmica na SIDA.

Imunoglobulina A secretora, proteínas totais e fluxo de saliva nas ulcerações aftosas recorrentes

Martinez,Kennedy de Oliveira; Mendes,Lauro Lúcio; Alves,José Bento
Fonte: ABORL-CCF Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial Publicador: ABORL-CCF Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2007 Português
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De etiologia discutível, as ulcerações aftosas recorrentes (UAR) são, na maioria das vezes, consideradas em um quadro de deficiência imunológica. OBJETIVOS: Este trabalho baseia-se na revisão de literatura e em investigações clínico-laboratoriais dos títulos salivares de imunoglobulina A secretora (IgA-s), proteínas totais e fluxo de saliva (em repouso) de pacientes nos períodos de atividade e quiescência das Ulcerações aftosas recorrentes. Forma de Estudo: Estudo clínico e experimental. MATERIAL E MÉTODOS: Empregou-se a Nefelometria na quantificação das IgA-s salivares, o vermelho de Pirogalol para as proteínas totais salivares e a análise gravimétrica para a determinação do fluxo de saliva. RESULTADOS: Os resultados demonstraram aumento estatisticamente significativo das IgA-s salivares nos períodos de lesão ativa em relação aos de quiescência. Não houve diferença estatística nas concentrações de proteínas totais e no fluxo de saliva. CONCLUSÃO: Sugere-se que a IgA-s pode ser usada como parâmetro do status imune da mucosa oral.

Diagnóstico da raiva canina: I. comparação entre amostras de saliva e de encéfalo

Côrtes,Valdson de Angelis; Paim,Gil Vianna; Oliveira,Maria Cecília Gibrail de
Fonte: Faculdade de Saúde Pública da Universidade de São Paulo Publicador: Faculdade de Saúde Pública da Universidade de São Paulo
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/1979 Português
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Foram examinadas mediante as técnicas de inoculação em camundongos e de imunofluorescência, 30 amostras de saliva e 30 amostras de encéfalo, colhidas de 30 cães raivosos. Todas as amostras, tanto de saliva como de encéfalo, apresentaram resultados positivos frente às duas técnicas usadas, demonstrando uma perfeita correlação entre os métodos e os espécimens utilizados. Foi ressaltada a importância da saliva para o estabelecimento de um diagnóstico mais precoce da raiva.

Ingestion of saliva during carbohydrate feeding by Lutzomyia longipalpis (Diptera; Psychodidae)

Cavalcante,Reginaldo R; Pereira,Marcos H; Freitas,Jorge M; Gontijo,Nelder de F
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2006 Português
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The aim of this study was to obtain experimental evidence that phlebotomine saliva is actually ingested during the carbohydrate ingestion phase (before and after blood digestion). The ingestion of carbohydrate was simulated as it occurs in the field by offering the insects balls of cotton soaked in sucrose, sucrose crystals or orange juice cells. The results obtained here showed that ingestion occurred under each condition investigated, as indicated by the presence of apyrase, an enzyme used as a marker to detect saliva in the insect gut and/or carbohydrate sources. Saliva ingestion by phlebotomine during the carbohydrate ingestion phase is important to explain how it could promote starch digestion and to trigger Leishmania promastigotes to follow a differentiation pathway as proposed previously by some authors.

Detection of human herpesvirus 6 and 7 DNA in saliva from healthy adults from Rio de Janeiro, Brazil

Magalhães,Ivna M; Martins,Rebeca VN; Cossatis,João J; Cavaliere,Renata M; Afonso,Larissa A; Moysés,Natalia; Oliveira,Solange A; Cavalcanti,Silvia MB
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/2010 Português
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In this study, we aimed to evaluate virus shedding in the saliva of healthy adults from the metropolitan region of the city of Rio de Janeiro, Brazil, in order to verify the prevalence of both human herpesviruses 6 and 7 (HHV-6, HHV-7). The studied group comprised 182 healthy individuals at Pedro Ernesto University Hospital, who were being seen for annual odontologic revisions. Saliva specimens were subjected to a multiplex polymerase chain reaction (PCR) to detect the presence of HHV-6A, HHV-6B and HHV-7. The total Roseolovirus DNA prevalence was 22.4%. The PCR detected a HHV-6 prevalence of 9.8%, with HHV-6A detected in 7.1% of the samples and HHV-6B in 2.7%. HHV-7 DNA was revealed in 12.6% of the studied cases. Multiple infections caused by HHV-6A and 7 were found in 2.1% of the samples. No statistical differences were observed regarding age, but for HHV-7 infection, an upward trend was observed in female patients. Compared to studies from other countries, low prevalence rates of herpesvirus DNA were detected in saliva from the healthy individuals in our sample. PCR methodology thus proved to be a useful tool for Roseolovirus detection and it is important to consider possible geographic and populations differences that could explain the comparatively low prevalence rates described here.

Estudo in vitro e ex vivo da ação de diferentes concentrações de extratos de própolis frente aos microrganismos presentes na saliva de humanos

Simões,Cinthia Coelho; Araújo,Danilo Barral de; Araújo,Roberto Paulo Correia de
Fonte: Sociedade Brasileira de Farmacognosia Publicador: Sociedade Brasileira de Farmacognosia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2008 Português
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Este estudo avaliou, in vitro e ex vivo, a ação de diferentes concentrações de extratos de própolis, comparativamente à eficácia de anti-sépticos bucais, frente aos microrganismos presentes na saliva de humanos. Para a realização dos ensaios in vitro, foi adicionada à saliva solução de glicose a 25%, seguindo-se da coleta de alíquotas para a preparação dos grupos controle (C) e experimentais, mediante a adição do extrato de própolis a 11%, 20% e 30%, e do Periogard, Listerine, Malvatricin e Parodontax. Nas experimentações ex vivo, foram coletadas amostras de saliva em jejum (C) e após o enxágüe individual com os extratos de própolis, seguindo-se da adição de glicose. Nas duas fases, foram recolhidas alíquotas das misturas e determinado o consumo de glicose pelos microrganismos pelo método Glicose Oxidase, nos tempos 0, 24, 48 h de incubação a 37 ºC. Constatou-se diferenças estatisticamente significantes no consumo de glicose aos serem comparadas as médias do grupo C nas fases 1 e 2 após 24 e 48 h. Entre os grupos experimentais, não foram constatadas diferenças significativas. Conclui-se que as soluções de própolis a 11%, 20% e 30% tiveram a mesma ação antimicrobiana, o que justifica a indicação daquela com menor concentração. Constatou-se também a mesma ação farmacológica em comparação aos anti-sépticos industrializados testados.

Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection

Souto,Renata; Silva-Boghossian,Carina M.; Colombo,Ana Paula Vieira
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2014 Português
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P. aeruginosa and Acinetobacter spp. are important pathogens associated with late nosocomial pneumonia in hospitalized and institutionalized individuals. The oral cavity may be a major source of these respiratory pathogens, particularly in the presence of poor oral hygiene and periodontal infection. This study investigated the prevalence of P. aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with periodontal disease or health. Samples were obtained from 55 periodontally healthy (PH) and 169 chronic periodontitis (CP) patients. DNA was obtained from the samples and detection of P. aeruginosa and Acinetobacter spp. was carried out by multiplex and nested PCR. P. aeruginosa and Acinetobacter spp. were detected in 40% and 45% of all samples, respectively. No significant differences in the distribution of these microorganisms between men and women, subgingival biofilm and saliva samples, patients < 35 and > 35 years of age, and smokers and non-smokers were observed regardless periodontal status (p > 0.05). In contrast, the frequencies of P. aeruginosa and Acinetobacter spp. in saliva and biofilm samples were significantly greater in CP than PH patients (p < 0.01). Smokers presenting P. aeruginosa and high frequencies of supragingival plaque were more likely to present CP than PH. P. aeruginosa and Acinetobacter spp. are frequently detected in the oral microbiota of CP. Poor oral hygiene...

Bond strength of resin-resin interfaces contaminated with saliva and submitted to different surface treatments

Furuse,Adilson Yoshio; Cunha,Leonardo Fernandes da; Benetti,Ana Raquel; Mondelli,José
Fonte: Faculdade De Odontologia De Bauru - USP Publicador: Faculdade De Odontologia De Bauru - USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2007 Português
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The purpose of this study was to investigate the effect of different surface treatments on shear bond strength of saliva-contaminated resin-resin interfaces. Flat resin surfaces were fabricated. In the control group, no contamination or surface treatment was performed. The resin surfaces of the experimental groups were contaminated with saliva and air-dried, and then submitted to: (G1) rinsing with water and drying; (G2) application of an adhesive system; (G3) rinsing and drying, abrasion with finishing disks, etching and application of adhesive system; (G4) rinsing and drying, etching, application of silane and adhesive system. Resin cylinders were placed over the treated surfaces. The specimens were stored in water or ethanol. Shear bond strength tests were performed and the mode of failure was evaluated. Data were submitted to two-way ANOVA and Dunnett T3 test. Contamination of resin-resin interfaces with saliva significantly reduced shear strength, especially after prolonged storage (p<0.05). Similar values to the original bond strength were obtained after abrasion and application of adhesive (G3) or etching and application of silane and adhesive (G4). If contamination occurs, a surface treatment is required to guarantee an adequate interaction between the resin increments.

Saliva and tongue coating pH before and after use of mouthwashes and relationship with parameters of halitosis

Tolentino,Elen de Souza; Chinellato,Luiz Eduardo Montenegro; Tarzia,Olinda
Fonte: Faculdade De Odontologia De Bauru - USP Publicador: Faculdade De Odontologia De Bauru - USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2011 Português
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OBJECTIVES: The aim of this work was to evaluate saliva and tongue coating pH in oral healthy patients with morning bad breath before and after use of different oral mouthrinses. MATERIAL AND METHODS: Saliva and tongue coating pH of 50 patients allocated in 5 groups were measured respectively by a digital pHmeter and color pH indicators, before, immediately after and 30 min after rinsing 5 different mouthrinses: cetilpiridine chloride associated with sodium chloride, triclosan, enzymatic solution, essential oil and distilled water. RESULTS: Only triclosan and essential oil increased salivary pH immediately after rising. The enzymatic solution decreased salivary and tongue coating pH immediately after rinsing. CONCLUSIONS: Salivary pH tended to be acidic while tongue coating pH tended to be alkaline, even after rising. Triclosan and essential oil mouthrinses increased salivary pH immediately after rinsing. Enzymatic solution decreased saliva and tongue coating pH immediately after rising.

Coronal microleakage of endodontically treated teeth with intracanal post exposed to fresh human saliva

Oliveira,Simone Gomes dias de; Gomes,Denise Jornada; Costa,Marcelo Hisse das Neves; Sousa,Ezilmara Rolim de; Lund,Rafael Guerra
Fonte: Faculdade De Odontologia De Bauru - USP Publicador: Faculdade De Odontologia De Bauru - USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2013 Português
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OBJECTIVE: The aim of this study was to investigate the coronal microleakage of endodontically treated teeth prepared to receive an intracanal post and teeth with an intracanal post but without a prosthetic crown and exposed to contamination by fresh human saliva. MATERIAL AND METHODS: A mechanical-chemical preparation following the step-back technique was carried out in 35 extracted single-rooted human teeth. The teeth were randomly divided into five groups: G1=root canals instrumented, obturated, and prepared to receive an intracanal post (N=10); G2=root canals with cemented posts but without coronal sealing (N=10); PC1=positive control root canals instrumented and open (N=5); PC2=positive control 2 root canals without instrumentation and open (N=5); and NC=negative control healthy teeth (N=5). The crowns were removed except for the control group of intact teeth. The root canals were obturated and sterilized with cobalt 60 gamma irradiation and were then adapted in an apparatus using a Brain Heart Infusion (BHI) medium and fresh human saliva for contamination. Microbial growth was indicated by the presence of turbidity in the BHI liquid medium. RESULTS: Data were submitted to the Kaplan-Meier Survival Analysis and the Holm-Sidak statistic method...

Calcium, amylase, glucose, total protein concentrations, flow rate, pH and buffering capacity of saliva in patients undergoing orthodontic treatment with fixed appliances

Teixeira,Hellen Soares; Kaulfuss,Stella Maris Oliveira; Ribeiro,Jucienne Salgado; Pereira,Betina do Rosário; Brancher,João Armando; Camargo,Elisa Souza
Fonte: Dental Press International Publicador: Dental Press International
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2012 Português
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OBJECTIVE: To evaluate qualitative and quantitative changes in the saliva of individuals undergoing orthodontic treatment with fixed appliances. METHODS: Salivary samples were collected from 50 individuals divided in two groups: Experimental Group - patients with fixed orthodontic appliances (n=25); and Control Group - subjects with no orthodontic appliances (n=25). Salivary flow rate, pH, buffering capacity, amylase activity, concentrations of total proteins, calcium and glucose were measured in all salivary samples. RESULTS: There was a reduction in salivary pH and buffering capacity and an increase in the concentration of calcium ions in the experimental group (p<0.05); there was also an increase in glucose, amylase and protein concentrations in the saliva of the Experimental Group, but the differences were insignificant. There was insignificant correlation between calcium ion concentration and salivary flow or between buffering capacity and salivary flow. CONCLUSION: The saliva of individuals with fixed orthodontic appliances had lower pH, buffering capacity and calcium concentration than that of individuals without any type of orthodontic appliance. These oral changes are enough to cause tooth demineralization. Patients with orthodontic appliances should adopt additional oral hygiene procedures.

Comparative analysis of the human saliva microbiome from different climate zones: Alaska, Germany, and Africa

Li, Jing; Quinque, Dominique; Horz, Hans-Peter; Li, Mingkun; Rzhetskaya, Margarita; Raff, Jennifer A; Hayes, M Geoffrey; Stoneking, Mark
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Português
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Background: Although the importance of the human oral microbiome for health and disease is increasingly recognized, variation in the composition of the oral microbiome across different climates and geographic regions is largely unexplored. Results: Here we analyze the saliva microbiome from native Alaskans (76 individuals from 4 populations), Germans (10 individuals from 1 population), and Africans (66 individuals from 3 populations) based on next-generation sequencing of partial 16S rRNA gene sequences. After quality filtering, a total of 67,916 analyzed sequences resulted in 5,592 OTUs (defined at ≥97% identity) and 123 genera. The three human groups differed significantly by the degree of diversity between and within individuals (e.g. beta diversity: Africans > Alaskans > Germans; alpha diversity: Germans > Alaskans > Africans). UniFrac, network, ANOSIM, and correlation analyses all indicated more similarities in the saliva microbiome of native Alaskans and Germans than between either group and Africans. The native Alaskans and Germans also had the highest number of shared bacterial interactions. At the level of shared OTUs, only limited support for a core microbiome shared across all three continental regions was provided, although partial correlation analysis did highlight interactions involving several pairs of genera as conserved across all human groups. Subsampling strategies for compensating for the unequal number of individuals per group or unequal sequence reads confirmed the above observations. Conclusion: Overall...

Análise dos níveis de endotelina-1 na saliva de pacientes portadores de carcinoma espinocelular bucal

Hoffmann, Renata da Rocha
Fonte: Pontifícia Universidade Católica do Rio Grande do Sul; Porto Alegre Publicador: Pontifícia Universidade Católica do Rio Grande do Sul; Porto Alegre
Tipo: Tese de Doutorado
Português
Relevância na Pesquisa
36.82%
Objetivos: Verificar se a Endotelina-1 (ET-1) salivar pode representar um biomarcador eficiente para avaliação de risco e prognóstico do carcinoma espinocelular bucal. Metodologia: Neste estudo, foi realizada coleta de saliva de 20 pacientes saudáveis, 15 pacientes portadores de leucoplasia oral (diagnóstico histopatológico de hiperceratose a displasia severa), 14 pacientes que já tiveram carcinoma espinocelular bucal (CEB) e estavam em um período pós-tratamento e livres de doença por, no mínimo cinco anos e, 20 pacientes portadores de CEB. A ET-1 foi quantificada na saliva através do método ELISA. Resultados: Não houve diferença estatisticamente significativa na expressão de ET-1 entre os grupos estudados, mesmo quando pacientes com idade superior a 65 anos ou hipertensos foram excluídos da análise. Da mesma forma, quando a amostra foi separada por sexo, também não houve diferença significativa. Conclusões: A ET-1 salivar não se mostrou um biomarcador eficiente do carcinoma espinocelular bucal.; Objectives: The present study aimed to verify if the salivary Endothelin-1 (ET-1) expression might represent an efficient biomarker to evaluate risk and prognosis from oral squamous cell carcinoma (OSCC).Methods: For the present study...