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Efeitos da farmacoterapia utilizando doses máximas de clopidogrel e atorvastatina no controle da hiperplasia neointimal pós-implante de stent coronário; Impact of optimized clopidogrel 150 mg and atorvastatin 80 mg treatment to control neointimal hyperplasia after PCI with bare metal stent: an intravascular ultrasound study

Pavanello, Ricardo
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 01/06/2012 Português
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Fundamentos: O implante de stents coronários constitui-se na técnica mais prevalente de revascularização percutânea, em especial pela prevenção da reestenose, quando comparado às intervenções com o balão. No entanto, a reestenose intra-stent, que ocorre em cerca de 25% dos casos, restringe os seus benefícios clínicos e econômicos tardios. Demonstrou-se que a hiperplasia neo-intimal decorrente da reação da parede vascular causada pelo implante do stent, é responsável pelas recidivas. Interroga-se se um protocolo de medicamentos contemplando doses máximas de manutenção de clopidogrel e atorvastatina poderia reduzir a hiperplasia neo-intimal e a reestenose. Objetivos: O objetivo primário foi aferir se esta associação de medicamentos reduziria o volume de hiperplasia neo-intimal (35% ou mais), expressa pela obstrução volumétrica da luz, mensurada pelo ultrassom intracoronário, 12 meses após a intervenção. Os objetivos secundários foram: os resultados da angiografia quantitativa e os eventos cardíacos adversos maiores (óbito, infarto e revascularização do vaso-alvo). Casuística e métodos: Foram incluídos casos eletivos e com lesões primárias nas artérias naturais. Os pacientes foram tratados com stents não farmacológicos e randomizados em dois grupos: A...

AVALIAÇÃO DO DESEMPENHO A LONGO PRAZO DA RECICLAGEM A FRIO DE PAVIMENTO, COM ADIÇÃO DE CIMENTO, DA RODOVIA SC 150, TRECHO BR 282 À CAPINZAL

Negrello, Camila Sorg
Fonte: Universidade Federal de Santa Catarina Publicador: Universidade Federal de Santa Catarina
Tipo: Trabalho de Conclusão de Curso Formato: 196 f.
Português
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36.23%
TCC (graduação) - Universidade Federal de Santa Catarina. Centro Tecnológico. Engenharia Civil.; A presente pesquisa tem como objetivo fazer a avaliação a longo prazo do desempenho de um segmento de 1600 (mil e seiscentos) metros da rodovia SC-150, localizada entre os municípios de Joaçaba e Capinzal em Santa Catarina. O trecho foi o primeiro no Estado a utilizar a técnica de restauração através de reciclagem com adição de cimento. O trecho foi aberto ao tráfego em 2007. Como subsídios para avaliação, serão utilizados dados de bacias de deflexão levantados nos anos de 2012 e 2014, além de dados de afundamento de trilha de roda de 2010, 2012 e 2014, irregularidade longitudinal de 2012 e 2014 e patologias de 2010, 2012 e 2014. Com estes dados será realizada a retroanálise para estimar módulos resilientes das camadas e fazer a análise tensão deformação com o software ELSYM5. Como base para o presente trabalho, serão utilizados os dados de Lorena (2009), primeiro estudo publicado sobre este segmento. A partir da análise de dados foi possível concluir que a base reciclada está em boas condições estruturais. As análises de deflexões, trilha de roda e irregularidade longitudinal mostram resultados bastante satisfatórios...

MicroRNAs miR-186 and miR-150 Down-regulate Expression of the Pro-apoptotic Purinergic P2X7 Receptor by Activation of Instability Sites at the 3′-Untranslated Region of the Gene That Decrease Steady-state Levels of the Transcript*

Zhou, Lingyin; Qi, Xiaoping; Potashkin, Judith A.; Abdul-Karim, Fadi W.; Gorodeski, George I.
Fonte: American Society for Biochemistry and Molecular Biology Publicador: American Society for Biochemistry and Molecular Biology
Tipo: Artigo de Revista Científica
Publicado em 17/10/2008 Português
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The P2X7 receptor regulates cell growth through mediation of apoptosis. P2X7 levels are lower in cancer epithelial cells than in normal cells, and previous studies showed that expression of P2X7 was regulated post-transcriptionally. The objective of the study was to understand regulation of P2X7 mRNA stability. Overexpression of a reporter containing the full-length human P2X7 3′-untranslated region (3′-UTR) or reporters containing parts of the 3′-UTR-P2X7 were associated with increased abundance of the construct in normal cells and decreased abundance in cancer epithelial cells. Sequences within the 3′-UTR-P2X7, which are putative target sites for the microRNAs, miR-186 (middle segment) and miR-150 (distal segment), decreased the abundance of the P2X7 transcript. Overexpression in cancer cells of mutated miR-186 and miR-150 target sites was associated with lower levels of the reporter genes. In normal cells overexpression of the mutated miR-186 target site was associated with marked increased concentration, but overexpression of the miR-150 target site reporters, wild-type and mutant, did not change over time. Levels of miR-186 and miR-150 were higher in cancer than in normal cells, and treatment with miR-186 and miR-150 inhibitors increased P2X7 mRNA. In human embryonic kidney-293 cells heterologously expressing the full-length 3′-UTR-P2X7 luciferase reporter...

microRNA-150 Regulates Mobilization and Migration of Bone Marrow-Derived Mononuclear Cells by Targeting Cxcr4

Tano, Nobuko; Kim, Ha Won; Ashraf, Muhammad
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 19/10/2011 Português
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The interaction between chemokine receptor type 4 (CXCR4) and its ligand, stromal cell-derived factor (SDF)-1, plays an important role in stem cell mobilization and migration in ischemic tissues. MicroRNAs (miRs) are key regulators of stem cell function and are involved in regulation of stem cell survival and differentiation to adopt different cell lineages. In this study, we show that ischemia inhibits the expression of miR-150 in BM-derived mononuclear cells (MNC) and activates its target Cxcr4 gene. Our results show that miR-150/CXCR4 cascade enhances MNC mobilization and migration. By using mouse acute myocardial infarction (MI) model, we found that MNCs in peripheral blood (PB) were increased significantly at day 5 after AMI as compared to control group and the number of CXCR4 positive MNCs both in bone marrow (BM) and PB was also markedly increased after MI. Analysis by microarray-based miRNA profiling and real-time PCR revealed that the expression of miR-150 which targets Cxcr4 gene as predicted was significantly downregulated in BM-MNCs after MI. Abrogation of miR-150 markedly increased CXCR4 protein expression suggesting its target gene. To show that miR-150 regulates MNC mobilization, knockdown of miR-150 in BM-MNCs by specific antisense inhibitor resulted in their higher migration ability in vitro as compared to scramble-transfected MNCs. Furthermore...

Plasticity of 150-Loop in Influenza Neuraminidase Explored by Hamiltonian Replica Exchange Molecular Dynamics Simulations

Han, Nanyu; Mu, Yuguang
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 10/04/2013 Português
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Neuraminidase (NA) of influenza is a key target for antiviral inhibitors, and the 150-cavity in group-1 NA provides new insight in treating this disease. However, NA of 2009 pandemic influenza (09N1) was found lacking this cavity in a crystal structure. To address the issue of flexibility of the 150-loop, Hamiltonian replica exchange molecular dynamics simulations were performed on different groups of NAs. Free energy landscape calculated based on the volume of 150-cavity indicates that 09N1 prefers open forms of 150-loop. The turn A (residues 147–150) of the 150-loop is discovered as the most dynamical motif which induces the inter-conversion of this loop among different conformations. In the turn A, the backbone dynamic of residue 149 is highly related with the shape of 150-loop, thus can function as a marker for the conformation of 150-loop. As a contrast, the closed conformation of 150-loop is more energetically favorable in N2, one of group-2 NAs. The D147-H150 salt bridge is found having no correlation with the conformation of 150-loop. Instead the intimate salt bridge interaction between the 150 and 430 loops in N2 variant contributes the stabilizing factor for the closed form of 150-loop. The clustering analysis elaborates the structural plasticity of the loop. This enhanced sampling simulation provides more information in further structural-based drug discovery on influenza virus.

miR-150 Promotes Renal Fibrosis in Lupus Nephritis by Downregulating SOCS1

Zhou, Hua; Hasni, Sarfaraz A.; Perez, Paola; Tandon, Mayank; Jang, Shyh-Ing; Zheng, Changyu; Kopp, Jeffery B.; Austin, Howard; Balow, James E.; Alevizos, Ilias; Illei, Gabor G.
Fonte: American Society of Nephrology Publicador: American Society of Nephrology
Tipo: Artigo de Revista Científica
Português
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26.54%
MicroRNAs (miRs) seem to mediate renal fibrosis in several renal diseases, with some miRs having profibrotic effects and others having opposing effects. Although differential expression of certain miRs has been described in lupus nephritis, it is unknown whether miRs contribute to fibrosis or could serve as biomarkers of specific histologic manifestations of lupus nephritis. Here, we compared miR expression in kidney biopsies from patients with lupus nephritis and identified miR-150 as the most differentially expressed miR in kidneys with high chronicity (chronicity index [CI] ≥4); miR-150 positively correlated with chronicity scores and the expression of profibrotic proteins. Overexpression of miR-150 significantly reduced expression of the antifibrotic protein suppressor of cytokine signaling 1 (SOCS1) and upregulated profibrotic proteins in both proximal tubular and mesangial cells. Directly targeting SOCS1 with a small interfering RNA produced similar results. Furthermore, TGF-β1 induced miR-150 expression, decreased SOCS1, and increased profibrotic proteins in proximal tubular cells and podocytes; a miR-150 inhibitor reversed these changes, suggesting that the profibrotic effects of TGF-β1 are, at least in part, mediated by miR-150. Consistent with these in vitro observations...

MicroRNA-150 Expression Induces Myeloid Differentiation of Human Acute Leukemia Cells and Normal Hematopoietic Progenitors

Morris, Valerie A.; Zhang, Ailin; Yang, Taimei; Stirewalt, Derek L.; Ramamurthy, Ranjani; Meshinchi, Soheil; Oehler, Vivian G.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 24/09/2013 Português
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In acute myeloid leukemia (AML) and blast crisis (BC) chronic myeloid leukemia (CML) normal differentiation is impaired. Differentiation of immature stem/progenitor cells is critical for normal blood cell function. MicroRNAs (miRNAs or miRs) are small non-coding RNAs that interfere with gene expression by degrading messenger RNAs (mRNAs) or blocking protein translation. Aberrant miRNA expression is a feature of leukemia and miRNAs also play a significant role in normal hematopoiesis and differentiation. We have identified miRNAs differentially expressed in AML and BC CML and identified a new role for miR-150 in myeloid differentiation. Expression of miR-150 is low or absent in BC CML and AML patient samples and cell lines. We have found that expression of miR-150 in AML cell lines, CD34+ progenitor cells from healthy individuals, and primary BC CML and AML patient samples at levels similar to miR-150 expression in normal bone marrow promotes myeloid differentiation of these cells. MYB is a direct target of miR-150, and we have identified that the observed phenotype is partially mediated by MYB. In AML cell lines, differentiation of miR-150 expressing cells occurs independently of retinoic acid receptor α (RARA) signaling. High-throughput gene expression profiling (GEP) studies of the AML cell lines HL60...

miR-150 Promotes Human Breast Cancer Growth and Malignant Behavior by Targeting the Pro-Apoptotic Purinergic P2X7 Receptor

Huang, Songyin; Chen, Yongsong; Wu, Wei; Ouyang, Nengyong; Chen, Jianing; Li, Hongyu; Liu, Xiaoqiang; Su, Fengxi; Lin, Ling; Yao, Yandan
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 02/12/2013 Português
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26.54%
The P2X7 receptor regulates cell growth through mediation of apoptosis. Low level expression of P2X7 has been linked to cancer development because tumor cells harboring a defective P2X7 mechanism can escape P2X7 pro-apoptotic control. microRNAs (miRNAs) function as negative regulators of post-transcriptional gene expression, playing major roles in cellular differentiation, proliferation, and metastasis. In this study, we found that miR-150 was over-expressed in breast cancer cell lines and tissues. In these breast cancer cell lines, blocking the action of miR-150 with inhibitors leads to cell death, while ectopic expression of the miR-150 results in increased cell proliferation. We deploy a microRNA sponge strategy to inhibit miR-150 in vitro, and the result demonstrates that the 3′-untranslated region (3′UTR) of P2X7 receptor contains a highly conserved miR-150-binding motif and its direct interaction with miR-150 down-regulates endogenous P2X7 protein levels. Furthermore, our findings demonstrate that miR-150 over-expression promotes growth, clonogenicity and reduces apoptosis in breast cancer cells. Meanwhile, these findings can be decapitated in nude mice with breast cancer xenografts. Finally, these observations strengthen our working hypothesis that up-regulation of miR-150 in breast cancer is inversely associated with P2X7 receptor expression level. Together...

Exposure to MIV-150 from a High-Dose Intravaginal Ring Results in Limited Emergence of Drug Resistance Mutations in SHIV-RT Infected Rhesus Macaques

Hsu, Mayla; Keele, Brandon F.; Aravantinou, Meropi; Krawczyk, Noa; Seidor, Samantha; Abraham, Ciby J.; Zhang, Shimin; Rodriguez, Aixa; Kizima, Larisa; Derby, Nina; Jean-Pierre, Ninochka; Mizenina, Olga; Gettie, Agegnehu; Grasperge, Brooke; Blanchard, Jame
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 27/02/2014 Português
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When microbicides used for HIV prevention contain antiretroviral drugs, there is concern for the potential emergence of drug-resistant HIV following use in infected individuals who are either unaware of their HIV infection status or who are aware but still choose to use the microbicide. Resistant virus could ultimately impact their responsiveness to treatment and/or result in subsequent transmission of drug-resistant virus. We tested whether drug resistance mutations (DRMs) would emerge in macaques infected with simian immunodeficiency virus expressing HIV reverse transcriptase (SHIV-RT) after sustained exposure to the potent non-nucleoside reverse transcriptase inhibitor (NNRTI) MIV-150 delivered via an intravaginal ring (IVR). We first treated 4 SHIV-RT-infected animals with daily intramuscular injections of MIV-150 over two 21 day (d) intervals separated by a 7 d drug hiatus. In all 4 animals, NNRTI DRMs (single and combinations) were detected within 14 d and expanded in proportion and diversity with time. Knowing that we could detect in vivo emergence of NNRTI DRMs in response to MIV-150, we then tested whether a high-dose MIV-150 IVR (loaded with >10 times the amount being used in a combination microbicide IVR in development) would select for resistance in 6 infected animals...

MIV-150-Containing Intravaginal Rings Protect Macaque Vaginal Explants against SHIV-RT Infection

Ouattara, Louise A.; Barnable, Patrick; Mawson, Paul; Seidor, Samantha; Zydowsky, Thomas M.; Kizima, Larisa; Rodriguez, Aixa; Fernández-Romero, José A.; Cooney, Michael L.; Roberts, Kevin D.; Gettie, Agegnehu; Blanchard, James; Robbiani, Melissa; Telesh
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /05/2014 Português
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26.58%
Recent studies demonstrated that intravaginal rings (IVRs) containing 100 mg of the nonnucleoside reverse transcriptase inhibitor (NNRTI) MIV-150 significantly protect macaques against a chimeric simian-human immunodeficiency virus that expresses the HIV-1 HxB2 reverse transcriptase (SHIV-RT) when present before and after vaginal challenge. The objectives of this study were to (i) evaluate the pharmacodynamics (PD) of MIV-150 in vaginal fluids (VF) and in ectocervical and vaginal tissues following 100-mg MIV-150 IVR exposure and to (ii) gain more insight whether pharmacokinetics (PK) of MIV-150 can predict PD. MIV-150 in VF collected at 1 day and 14 days post-MIV-150 IVR insertion inhibited ex vivo SHIV-RT infection in vaginal biopsy specimens from untreated animals (not carrying IVRs) in a dose-dependent manner. Previous PK studies demonstrated a significant increase of ectocervical and vaginal tissue MIV-150 concentrations 14 days versus 1 day post-IVR insertion, with the highest increase in vaginal tissue. Therefore, we tested PD of MIV-150 in tissues 14 days post-MIV-150 IVR insertion. Ex vivo SHIV-RT infection of vaginal, but not ectocervical, tissues collected 14 days post-MIV-150 IVR insertion was significantly inhibited compared to infection at the baseline (prior to MIV-150 IVR exposure). No changes in vaginal and ectocervical tissue infection were observed after placebo IVR exposure. Overall...

Synthesis, characterization, and evaluation of poly (D,L-lactide-co-glycolide)-based nanoformulation of miRNA-150: potential implications for pancreatic cancer therapy

Arora, Sumit; Swaminathan, Suresh K; Kirtane, Ameya; Srivastava, Sanjeev K; Bhardwaj, Arun; Singh, Seema; Panyam, Jayanth; Singh, Ajay P
Fonte: Dove Medical Press Publicador: Dove Medical Press
Tipo: Artigo de Revista Científica
Publicado em 18/06/2014 Português
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26.57%
MicroRNAs are small (18–22 nucleotide long) noncoding RNAs that play important roles in biological processes through posttranscriptional regulation of gene expression. Their aberrant expression and functional significance are reported in several human malignancies, including pancreatic cancer. Recently, we identified miR-150 as a novel tumor suppressor microRNA in pancreatic cancer. Furthermore, expression of miR-150 was downregulated in the majority of tumor cases, suggesting that its restoration could serve as an effective approach for pancreatic cancer therapy. In the present study, we developed a nanoparticle-based miR-150 delivery system and tested its therapeutic efficacy in vitro. Using double emulsion solvent evaporation method, we developed a poly (D,L-lactide-co-glycolide) (PLGA)-based nanoformulation of miR-150 (miR-150-NF). Polyethyleneimine (a cationic polymer) was incorporated in PLGA matrix to increase the encapsulation of miR-150. Physical characterization of miR-150-NF demonstrated that these nanoparticles had high encapsulation efficiency (~78%) and exhibited sustained release profile. Treatment of pancreatic cancer cells with miR-150-NF led to efficient intracellular delivery of miR-150 mimics and caused significant downregulation of its target gene (MUC4) expression. Inhibition of MUC4 correlated with a concomitant decrease in the expression of its interacting partner...

MicroRNA-150 Predicts a Favorable Prognosis in Patients with Epithelial Ovarian Cancer, and Inhibits Cell Invasion and Metastasis by Suppressing Transcriptional Repressor ZEB1

Jin, Minfei; Yang, Zujing; Ye, Weiping; Xu, Hongling; Hua, Xiaolin
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 04/08/2014 Português
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MicroRNA (miR)-150 has been reported to be dramatically downregulated in human epithelial ovarian cancer (EOC) tissues and patients’ serum compared to normal controls. This study aimed to investigate clinical significance and molecular mechanisms of miR-150 in EOC. In the current study, quantitative real-time PCR analysis showed that miR-150 was significantly downregulated in human EOC tissues compared to normal tissue samples. Then, we demonstrated the significant associations of miR-150 downregulation with aggressive clinicopathological features of EOC patients, including high clinical stage and pathological grade, and shorter overall and progression-free survivals. More importantly, the multivariate analysis identified miR-150 expression as an independent prognostic biomarker in EOC. After that, luciferase reporter assays demonstrated that Zinc Finger E-Box Binding Homeobox 1 (ZEB1), a crucial regulator of epithelial-to-mesenchymal transition (EMT), was a direct target of miR-150 in EOC cells. Moreover, we found that the ectopic expression of miR-150 could efficiently inhibit cell proliferation, invasion and metastasis by suppressing the expression of ZEB1. Furthermore, we also observed a significantly negative correlation between miR-150 and ZEB1 mRNA expression in EOC tissues (rs = –0.45...

Ectopic MicroRNA-150-5p Transcription Sensitizes Glucocorticoid Therapy Response in MM1S Multiple Myeloma Cells but Fails to Overcome Hormone Therapy Resistance in MM1R Cells

Palagani, Ajay; Op de Beeck, Ken; Naulaerts, Stefan; Diddens, Jolien; Sekhar Chirumamilla, Chandra; Van Camp, Guy; Laukens, Kris; Heyninck, Karen; Gerlo, Sarah; Mestdagh, Pieter; Vandesompele, Joke; Berghe, Wim Vanden
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 04/12/2014 Português
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26.55%
Glucocorticoids (GCs) selectively trigger cell death in the multiple myeloma cell line MM1S which express NR3C1/Glucocorticoid Receptor (GR) protein, but fail to kill MM1R cells which lack GR protein. Given recent demonstrations of altered microRNA profiles in a diverse range of haematological malignancies and drug resistance, we characterized GC inducible mRNA and microRNA transcription profiles in GC sensitive MM1S as compared to GC resistant MM1R cells. Transcriptome analysis revealed that GCs regulate expression of multiple genes involved in cell cycle control, cell organization, cell death and immunological disease in MM1S cells, which remain unaffected in MM1R cells. With respect to microRNAs, mir-150-5p was identified as the most time persistent GC regulated microRNA, out of 5 QPCR validated microRNAs (mir-26b, mir-125a-5p, mir-146-5p, mir-150-5p, and mir-184), which are GC inducible in MM1S but not in MM1R cells. Functional studies further revealed that ectopic transfection of a synthetic mir-150-5p mimics GR dependent gene expression changes involved in cell death and cell proliferation pathways. Remarkably, despite the gene expression changes observed, overexpression of mir-150-5p in absence of GCs did not trigger significant cytotoxicity in MM1S or MM1R cells. This suggests the requirement of additional steps in GC induced cell death...

miR-150-5p Inhibits Hepatoma Cell Migration and Invasion by Targeting MMP14

Li, Tao; Xie, Junjie; Shen, Chuan; Cheng, Dongfeng; Shi, Yuan; Wu, Zhichong; Zhan, Qian; Deng, Xiaxing; Chen, Hao; Shen, Baiyong; Peng, Chenghong; Li, Hongwei; Zhu, Zhecheng
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 30/12/2014 Português
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26.54%
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. Despite progress in diagnostics and treatment of HCC, its prognosis remains poor because the molecular mechanisms underlying hepatocarcinogenesis are not well understood. In the study, we focused on identifying the role of miRNAs in HCC progression. miRNA microarray was used to analyze the differentially expressed miRNAs, and the results were validated by qPCR. We found that the miR-150-5p expression is down-regulated in HCC tissues compared with pair non-tumor tissues. miR-150-5p expression is also decreased in metastatic cancer tissues compared with pair primary tissues, indicating that miR-150-5p may be involved in HCC metastasis. Functionally, miR-150-5p inhibition significantly promotes hepatoma cell migration and invasion, whereas miR-150-5p overexpression suppresses cancer cell migration and invasion in vitro. The matrix metalloproteinase 14 (MMP14) is identified as a new target gene of miR-150-5p. miR-150-5p markedly inhibits MMP14 expression in hepatoma cells, and miR-150-5p expression is negative correlation with MMP14 expression in vivo. More important, re-expression of MMP14 in hepatoma cells partially reverses the effect of miR-150-5p in inhibiting cell invasion.

Opposite Prognostic Significance of Cellular and Serum Circulating MicroRNA-150 in Patients with Chronic Lymphocytic Leukemia

Stamatopoulos, Basile; Van Damme, Michaël; Crompot, Emerence; Dessars, Barbara; Housni, Hakim El; Mineur, Philippe; Meuleman, Nathalie; Bron, Dominique; Lagneaux, Laurence
Fonte: ScholarOne Publicador: ScholarOne
Tipo: Artigo de Revista Científica
Publicado em 09/01/2015 Português
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26.54%
MicroRNAs (or miRs) play a crucial role in chronic lymphocytic leukemia (CLL) physiopathology and prognosis. In addition, circulating microRNAs in body fluids have been proposed as new biomarkers. We investigated the expression of matched cellular and serum circulating microRNA-150 by quantitative real-time PCR (qPCR) from purified CD19+ cells or from CLL serums obtained at diagnosis in a cohort of 273/252 CLL patients with a median follow-up of 78 months (range 7–380) and correlated it to other biological or clinical parameters. We showed that miR-150 was significantly overexpressed in CLL cells/serums compared with healthy subjects (P < 0.0001). Among CLL patients, a low cellular miR-150 expression level was associated with tumor burden, disease aggressiveness and poor prognostic factors. In contrast, a high level of serum miR-150 was associated with tumor burden markers and some markers of poor prognosis. Similarly, cellular and serum miR-150 also predicted treatment-free survival (TFS) and overall survival (OS) in an opposite manner: patients with low cellular/serum miR-150 levels have median TFS of 40/111 months compared with high-level patients who have a median TFS of 122/60 months (P < 0.0001/P = 0.0066). Similar results were observed for OS. We also found that cellular and serum miR-150 levels vary in an opposite manner during disease progression and that cellular miR-150 could be regulated by its release into the extracellular space. Cellular and serum levels of miR-150 are associated with opposite clinical prognoses and could be used to molecularly monitor disease evolution as a new prognostic factor in CLL.

Vila Arraias: espa?o de sobreviv?ncia, morte e n?cleo de organiza??o na luta pela terra na PA - 150 (1970-1985)

SANTOS, Edileuza dos
Fonte: Universidade Federal do Pará Publicador: Universidade Federal do Pará
Tipo: Dissertação de Mestrado
Português
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36.5%
Esta disserta??o procura compreender a hist?ria da vila Arraias (sudeste paraense) como constru??o do migrante-posseiro na d?cada de 70, buscando uma compreens?o de como ocorreu o processo de forma??o da vila ?s margens da PA-150, identificando e caracterizando os motivos e sujeitos que tiveram direta e indiretamente envolvidos nesse processo e quais foram suas a??es. A constru??o da rodovia estadual PA-150 no final da d?cada de 70, o processo migrat?rio que se direcionou para essa regi?o nas d?cadas de 70 e 80, ou seja, o processo de ocupa??o das margens dessa estrada por migrante-posseiro bem como os conflitos travados pela posse de terras nessa regi?o e a funcionalidade da vila Arraias como um n?cleo, uma c?lula na luta pela terra na regi?o da PA-150 ? o que d? dire??o e estrutura a esta discuss?o.; ABSTRACT: This dissertation tries to understand the history of Arraias village (southeast paraense) as construction of the migrant-leaseholding in the decade of 70, looking for an understanding of as it happened the process of formation of the village at the margins of the PA-150, identifyng and characterizing the reasons and subjects that had direct and indirectly involved in that process and which were their actions. The construction of the state highway PA-150 in the end of the decade of 70 and 80...

Identification of the HeLa tumor-associated antigen, p75/150, as intestinal alkaline phosphatase and evidence for its transcriptional regulation.

Latham, K M; Stanbridge, E J
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /02/1990 Português
Relevância na Pesquisa
26.54%
Prior studies identified a cell-surface antigen, p75/150, that exclusively associated with the tumorigenic phenotype of the HeLa parent and the tumorigenic phenotype of the HeLa parent and the tumorigenic segregants of suppressed, nontumorigenic HeLa x human fibroblast cell hybrids. Candidate p75/150 cDNA clones were isolated from a D98/AH.2 (HeLa) cDNA library using oligonucleotide probes derived from p75/150 partial peptide sequence data. A data base search revealed close similarity of p75/150 with intestinal alkaline phosphatase (IAP) [Berger, J., Garantini, E., Hua, J. C. & Udenfriend, S. (1987) Proc. Natl. Acad. Sci. USA 84, 695-698]. We demonstrate that p75/150 is identical to HeLa IAP by the following criteria: (i) 47/49 amino acid identity of p75 peptide sequence with IAP, (ii) restriction maps for the p75/150 candidate cDNA clone and IAP are identical, (iii) partial DNA sequence analysis of p75/150 candidate cDNA clones revealed complete nucleotide identity with IAP, except for a single nucleotide substitution in the 5' untranslated region, (iv) transfection of a p75/150 cDNA expression vector into the nontumorigenic hybrid, CGL1, yielded p75/150 antibody-positive transfectants that also expressed partially heat-resistant alkaline phosphatase activity. Northern blot analysis demonstrated that high levels of HeLa IAP mRNA were expressed in D98/AH.2 and the tumorigenic segregant CGL4; however...

The $^{150}$Nd($^3$He,$t$) and $^{150}$Sm($t$,$^3$He) reactions with applications to $\beta\beta$ decay of $^{150}$Nd

Guess, C. J.; Adachi, T.; Akimune, H.; Algora, A.; Austin, Sam M.; Bazin, D.; Brown, B. A.; Caesar, C.; Deaven, J. M.; Ejiri, H.; Estevez, E.; Fang, D.; Faessler, A.; Frekers, D.; Fujita, H.; Fujita, Y.; Fujiwara, M.; Grinyer, G. F.; Harakeh, M. N.; Hatan
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Publicado em 03/05/2011 Português
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The $^{150}$Nd($^3$He,$t$) reaction at 140 MeV/u and $^{150}$Sm($t$,$^3$He) reaction at 115 MeV/u were measured, populating excited states in $^{150}$Pm. The transitions studied populate intermediate states of importance for the (neutrinoless) $\beta\beta$ decay of $^{150}$Nd to $^{150}$Sm. Monopole and dipole contributions to the measured excitation-energy spectra were extracted by using multipole decomposition analyses. The experimental results were compared with theoretical calculations obtained within the framework of Quasiparticle Random-Phase Approximation (QRPA), which is one of the main methods employed for estimating the half-life of the neutrinoless $\beta\beta$ decay ($0\nu\beta\beta$) of $^{150}$Nd. The present results thus provide useful information on the neutrino responses for evaluating the $0\nu\beta\beta$ and $2\nu\beta\beta$ matrix elements. The $2\nu\beta\beta$ matrix element calculated from the Gamow-Teller transitions through the lowest $1^{+}$ state in the intermediate nucleus is maximally about half of that deduced from the half-life measured in $2\nu\beta\beta$ direct counting experiments and at least several transitions through $1^{+}$ intermediate states in $^{150}$Pm are required to explain the $2\nu\beta\beta$ half-life. Because Gamow-Teller transitions in the $^{150}$Sm($t$...

Inverse rules of ECA with rule number 150

Hernández Encinas, Luis; Martín del Rey, Ángel
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 579917 bytes; image/jpeg
Português
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The explicit expressions of local transition functions of the inverse cellular automata of elementary cellular automata with rule number 150 and periodic boundary conditions are given. The procedure to obtain such local transition functions is based on the algebraic properties of elementary cellular automata.; This work has been partially supported by the Consejería de Educación y Cultura of Junta de Castilla y Leónn (Spain) under Grant SA110A06, and by Ministerio de Educación y Ciencia (Spain), under Grant SEG2004-02418.m; Peer reviewed

Double-Beta Decay of 150Nd to Excited Final States

Kidd, Mary Frances
Fonte: Universidade Duke Publicador: Universidade Duke
Tipo: Dissertação Formato: 2939020 bytes; application/pdf
Publicado em //2010 Português
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An experimental study of the two-neutrino double-beta (2νββ) decay of 150Nd to various excited final states of 150Sm was performed at Triangle Universities Nuclear Laboratory (TUNL). Such data provide important checks for theoretical models used to predict 0νββ decay half lives.

The measurement was performed at the recently established Kimballton Underground Research Facility (KURF) in Ripplemeade, Virginia using the TUNL-ITEP double-beta decay setup. In this setup, two high-purity germanium detectors were operated in coincidence to detect the deexcitation gamma rays of the daughter nucleus. This coincidence technique, along with the location underground, provides a considerable reduction in background in the regions of interest.

This study yields the first results from KURF and the first detection of the

coincidence gamma rays from the 0+1 excited state of 150Sm. These gamma rays

have energies of 334.0 keV and 406.5 keV, and are emitted in coincidence through a 0+1→2+1→0+gs transition. The enriched Nd2O3 sample obtained from Oak Ridge

National Laboratory consists of 40.13 g 150Nd. This sample was observed for 391 days...