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Análise preliminar de riscos para montagem e desmontagem de proteção de periferia; Preliminary analysis of risks to assembly and disassembly of protection from periphery

Buss, Maurício Pereira
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Trabalho de Conclusão de Curso Formato: application/pdf
Português
Relevância na Pesquisa
26.73%
Análise preliminar de riscos é uma metodologia que possibilita identificar potenciais perigos em uma determinada atividade sendo um documento obrigatório em qualquer sistema de gestão de segurança do trabalho. A "NR-35" (Norma Regulamentadora n°35 - Trabalho em Altura), publicada no "D.O.U" (Diário Oficial da União) no dia 27 de março de 2012 e como prazo de vigência de 6 meses, a partir do dia 27 de setembro de 2012, regulamenta as atividades em altura e exige a análise preliminar de riscos para todas as atividades com altura superior a 2 metros em que haja risco de queda. O presente trabalho tem como objetivo apresentar uma "APR" (Análise Preliminar de Riscos) na atividade de montagem e desmontagem das proteções de periferia, modelo guarda-corpo-rodapé, na indústria da construção civil. Estas proteções são exigidas pela "NR 18" (Norma Regulamentadora n°18 - Condições e Meio Ambiente de Trabalho na Indústria da Construção), se encontram detalhadas na "RTP-01" (Recomendação Técnica de Procedimento - Medidas de Proteção Contra Quedas de Altura) da Fundacentro. A escolha do tema se dá em razão de alguns fatores: a proximidade do término do prazo de adequação das condições de trabalho às determinações da NR 35; a possibilidade de situações de grave e iminente risco (GIR) presentes em atividades em altura...

A importância da electroretinografia para o diagnóstico de retinopatias caninas

Lima, Clara Madureira
Fonte: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária Publicador: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária
Tipo: Trabalho de Conclusão de Curso
Publicado em 05/04/2011 Português
Relevância na Pesquisa
26.34%
Dissertação de Mestrado Integrado em Medicina Veterinária; Introdução: A electroretinografia de campo total por flash (ERGf) é afectada por múltiplas variáveis biológicas e ambientais. Para alcançar resultados viáveis e reproduzíveis, o clínico deve: estabelecer valores controlo para os parâmetros avaliados (amplitue e tempo implícito das ondas a e b), a partir de indivíduos saudáveis categorizados por raça e idade e para o seu equipamento e condições de trabalho; deve ser consistente na técnica de electroretinografia seguida (incluindo o material utilizado, protocolo anestésico e procurando manter constantes as variáveis ambientais) e cumprir sistematicamente o mesmo protocolo de ERG. Aqueles indivíduos que apresentam ausência ou redução na amplitude e tempo de latência das ondas a e b não são candidatos a facoemulsificação, dado que estes resultados são compatíveis com DSAR ou APR e hà um compromisso da função retiniana nestas situações. Objectivo: Discussão dos resultados electroretinográficos obtidos a partir de sete cães que se apresentaram à consulta de Oftalmologia do HVR entre Setembro de 2009 e Fevereiro de 2010. Cinco cães eram candidatos a facoemulsificação e dois apresentaram história de perda progressiva ou súbita de visão. Métodos: Na realização dos ERGf foi realizado o protocolo curto do ECVO e foram avaliadas a amplitude...

Mutagenesis of Erwinia carotovora subsp. carotovora with bacteriophage Mu d1 (Apr lac cts62): construction of his-lac gene fusions.

Jayaswal, R K; Bressan, R A; Handa, A K
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/1984 Português
Relevância na Pesquisa
26.34%
The bacteriophage Mu d1(Apr lac cts62 ) obtained from an Escherichia coli double lysogen carrying the defective Mu d1 phage and a Mu-P1 hybrid phage was utilized as a vector for phage mutagenesis in Erwinia carotovora subsp. carotovora. Among ampicillin-resistant transductants. 1.4% were auxotrophs. The synthesis of beta-galactosidase was derepressed upon starvation for histidine in two different his-lac fusion strains.

Infection of Salmonella typhimurium with coliphage Mu d1 (Apr lac): construction of pyr::lac gene fusions.

Csonka, L N; Howe, M M; Ingraham, J L; Pierson, L S; Turnbough, C L
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /01/1981 Português
Relevância na Pesquisa
26.34%
A procedure was developed for introducing the coliphage Mu d1 (Apr lac) into Salmonella typhimurium in order to construct gene fusions that place the structural genes of the lac operon under the control of the promoter-regulatory region of other genes. To introduce Mu d1 from Escherichia coli K-12 into S. typhimurium, which is normally not a host for Mu, we first constructed an E. coli double lysogen carrying the defective Mu d1 phage and a Mu-P1 hybrid helper phage (MuhP1) that confers the P1 host range. A lysate prepared from this strain was used to infect a P1-sensitive (i.e., galE), restriction-deficient, modification-proficient strain of S. typhimurium, and a double lysogen carrying Mu d1 and MuhP1 was isolated. Induction of the latter strain produced lysates capable of infecting and generating gene fusions in P1-sensitive strains of S. typhimurium. In this paper we describe the construction of pyr::lac fusions by this technique.

Roles for menaquinone and the two trimethylamine oxide (TMAO) reductases in TMAO respiration in Salmonella typhimurium: Mu d(Apr lac) insertion mutations in men and tor.

Kwan, H S; Barrett, E L
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/1983 Português
Relevância na Pesquisa
26.34%
Three groups of mutants defective in trimethylamine oxide (TMAO) reduction were isolated from Salmonella typhimurium LT2 subjected to transposition mutagenesis with Mu d(Apr lac). Mutants were identified by their acidic reaction on a modified MacConkey-TMAO medium. Group I consisted of pleiotropic chlorate-resistant mutants which were devoid of TMAO reductase activity. None expressed the lac operon. Group II mutants were partially defective in TMAO reductase. Electrophoretic studies revealed that they lacked the inducible TMAO reductase, but retained the constitutive activity. The genotypic designation tor was suggested for these mutants. The tor mutation in one was located between 80 and 83 U on the S. typhimurium chromosome. Expression of the lac operon in these mutants was not affected by air, TMAO, or nitrate. Group III mutants reduced little or no TMAO in vivo, but their extracts retained full capacity to reduce it with methyl viologen. These mutants also failed to produce hydrogen sulfide from thiosulfate and could not grow anaerobically on glycerol-fumarate. Two subgroups were distinguished. Vitamin K5 restored wild-type phenotype in subgroup IIIa only; vitamin K1 restored wild-type phenotype in both IIIa and IIIb isolates. The genotypic designation men (menaquinone) was suggested for group III isolates. The mutation in IIIa mutants was cotransducible with glpT...

Mu dX, a derivative of Mu d1 (lac Apr) which makes stable lacZ fusions at high temperature.

Baker, T A; Howe, M M; Gross, C A
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1983 Português
Relevância na Pesquisa
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We describe defective Mu phage Mu dX (Mu d1 Bx::Tn9 [lac Apr Cmr]) which is useful for insertion mutagenesis and for construction of lac operon fusions in vivo. Mu dX retains the insertion properties of Mu d1 but produces temperature-resistant lysogens and transposes at a reduced frequency. A method is described to convert existing Mu d1 insertions to Mu dX.

Bacteriophage Mu d1(Apr lac) generates vir-lac operon fusions in Shigella flexneri 2a.

Maurelli, A T; Curtiss, R
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/1984 Português
Relevância na Pesquisa
26.87%
Previous studies have demonstrated that expression of virulence in Shigella spp. is controlled by growth temperature. To study the regulation of virulence (vir) genes, we set out to develop a rapid, easily-assayed phenotype with which to measure expression of virulence. This report described a procedure for isolating vir-lac operon fusions in S. flexneri 2a by using the specialized transducing bacteriophage Mu d1(Apr lac) of Casadaban and Cohen (M. Casadaban and S. N. Cohen, Proc. Natl. Acad. Sci. U.S.A. 76:4530-4533, 1976). Mu d1(Apr lac) lysogens were isolated and screened for loss of virulence and for temperature-dependent expression of the lactose genes on Mu d1(Apr lac). A recombinant plasmid carrying the Mu immunity gene was also introduced into lysogens of interest to stabilize the Mu d1(Apr lac) insertion and prevent possible thermal induction at 37 degrees C. The mutant which we isolated failed to penetrate tissue culture cells in the assay for virulence and produced almost 15-fold more beta-galactosidase when grown at 37 degrees C than when grown at 30 degrees C. The site of insertion of Mu d1(Apr lac) in this strain was shown to be in the 140-megadalton plasmid pSf2a140, which is known to be associated with virulence. P1L4-mediated transduction of the insertion into a virulent recipient demonstrated genetic linkage of Mu d1(Apr lac) with loss of virulence and temperature-dependent expression of beta-galactosidase. All of these features fulfill the phenotype expected for a Mu d1(Apr lac)-induced vir-lac operon fusion. This mutant provides us with a means of measuring expression of a gene function required for virulence by assaying for beta-galactosidase. The insertion will also serve as a starting point for mapping of genes on pSf2a140 which are necessary for expression of virulence.

Isolation of ara-lac gene fusions in Salmonella typhimurium LT2 by using transducing bacteriophage Mu d (Apr lac).

Lee, J H; Heffernan, L; Wilcox, G
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/1980 Português
Relevância na Pesquisa
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A specialized Mu transducing phage containing a gene encoding ampicillin resistance and the lac structural genes without the lac promotor [Mu d(apr lac)] has been constructed and used to create gene fusions in Escherichia coli (M. J. Cadadaban and S. N. Cohen, Proc. Natl. Acad. Sci. U.S.A. 76:4530--4533, 1979). Transposition of the Mu d(Apr lac) phage to chromosomal sites can result in lac expression being controlled by a chromosomal promoter. We have constructed an Escherichia coli K-12 strain in which the Mu d(Apr lac) phage is integrated into an F factor. The F+::Mu d(Apr lac) was then transferred by conjugation into a Salmonella typhimurium strain that was sensitive to L-arabinose. Strains containing gene fusions were selected as L-arabinose-resistant colonies after partial induction of the phage. Two classes of ara-lac fusion strains were isolated: (i) araC-lac fusions in which the expression of beta-galactosidase synthesis was constitutuve and not inducible by L-arabinose; and ((ii) fusion of the lac genes to the ara structural genes in which the expression of beta-galatosidase synthesis was induced 263-fold by L-arabinose.

The Caenorhabditis elegans APC-related gene apr-1 is required for epithelial cell migration and Hox gene expression

Hoier, Erika Fröhli; Mohler, William A.; Kim, Stuart K.; Hajnal, Alex
Fonte: Cold Spring Harbor Laboratory Press Publicador: Cold Spring Harbor Laboratory Press
Tipo: Artigo de Revista Científica
Publicado em 01/04/2000 Português
Relevância na Pesquisa
26.89%
Inactivation of the Caenorhabditis elegans APC-related gene (apr-1) has pointed at two separate functions of apr-1. First, apr-1 is required for the migration of epithelial cells during morphogenesis of the embryo. In this process, APR-1 may act in a Cadherin/α-Catenin/β-Catenin complex as a component of adherens junctions. Second, apr-1 is required for Hox gene expression, most likely by positively regulating the activity of the Wingless signaling pathway. During embryogenesis, apr-1 is required for the expression of ceh-13 labial in anterior seam and muscle cells and during larval development, apr-1 is necessary for the expression of lin-39 deformed in the vulval precursor cells. Thus, APR-1 may positively regulate the activity of the β-Catenin/Armadillo-related proteins HMP-2 in migrating epithelial cells and BAR-1 in the vulval precursor cells.

Ov-APR-1, an aspartic protease from the carcinogenic liver fluke, Opisthorchis viverrini: functional expression, immunolocalization and subsite specificity

Suttiprapa, Sutas; Mulvenna, Jason; Huong, Ngo Thi; Pearson, Mark S.; Brindley, Paul J.; Laha, Thewarach; Wongkham, Sopit; Kaewkes, Sasithorn; Sripa, Banchob; Loukas, Alex
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
26.73%
The human liver fluke Opisthorchis viverrini is endemic in Thailand, Laos and Cambodia where long standing infection is associated with cancer of the bile ducts, cholangiocarcinoma. Here we describe a cathepsin D-like aspartic protease from the gut and other tissues in O. viverrini. Phylogenetic analysis indicated that Ov-APR-1 is cathepsin D-like, conforming with Clan AA, Family A1 of the MEROPS classification. Ov-APR-1 is expressed in the gut of the mature hermaphroditic parasite, in the reproductive tissues including the testis and immature spermatids, and the developing miracidium within the eggshell. The enzyme was also detected in the excretory/secretory products of cultured adult flukes, indicating a role in host-parasite relationships. A recombinant form of the enzyme expressed in Escherichia coli and refolded from denatured inclusion bodies underwent autocatalytic activation and demonstrated hydrolytic activity against the peptide substrate 7-methoxycoumarin-4-acetyl-GKPILFFRLK(DNP)-D-Arg-amide with a kcat/Km = 1.7 × 104 M−1s−1 and a pH optimum around pH 2.5–3.0. The recombinant enzyme digested hemoglobin and bovine serum albumin. Forty-six serum albumin peptides were detected after digestion with recombinant Ov-APR-1 and sequenced. Like many other aspartic proteases...

Sulphur flux through the sulphate assimilation pathway is differently controlled by adenosine 5′-phosphosulphate reductase under stress and in transgenic poplar plants overexpressing γ-ECS, SO, or APR

Scheerer, Ursula; Haensch, Robert; Mendel, Ralf R.; Kopriva, Stanislav; Rennenberg, Heinz; Herschbach, Cornelia
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
26.95%
Sulphate assimilation provides reduced sulphur for the synthesis of cysteine, methionine, and numerous other essential metabolites and secondary compounds. The key step in the pathway is the reduction of activated sulphate, adenosine 5′-phosphosulphate (APS), to sulphite catalysed by APS reductase (APR). In the present study, [35S]sulphur flux from external sulphate into glutathione (GSH) and proteins was analysed to check whether APR controls the flux through the sulphate assimilation pathway in poplar roots under some stress conditions and in transgenic poplars. (i) O-Acetylserine (OAS) induced APR activity and the sulphur flux into GSH. (ii) The herbicide Acetochlor induced APR activity and results in a decline of GSH. Thereby the sulphur flux into GSH or protein remained unaffected. (iii) Cd treatment increased APR activity without any changes in sulphur flux but lowered sulphate uptake. Several transgenic poplar plants that were manipulated in sulphur metabolism were also analysed. (i) Transgenic poplar plants that overexpressed the γ-glutamylcysteine synthetase (γ-ECS) gene, the enzyme catalysing the key step in GSH formation, showed an increase in sulphur flux into GSH and sulphate uptake when γ-ECS was targeted to the cytosol...

APR-246/PRIMA-1MET inhibits thioredoxin reductase 1 and converts the enzyme to a dedicated NADPH oxidase

Peng, X; Zhang, M-Q-Z; Conserva, F; Hosny, G; Selivanova, G; Bykov, V J N; Arnér, E S J; Wiman, K G
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
26.94%
The low-molecular-weight compound APR-246 (PRIMA-1MET) restores wild-type conformation and function to mutant p53, and triggers apoptosis in tumor cells. We show here that APR-246 also targets the selenoprotein thioredoxin reductase 1 (TrxR1), a key regulator of cellular redox balance. APR-246 inhibited both recombinant TrxR1 in vitro and TrxR1 in cells. A Sec-to-Cys mutant of TrxR1 was not inhibited by APR-246, suggesting targeting of the selenocysteine residue in wild-type TrxR1. Preheated APR-246 and its conversion product methylene quinuclidinone (MQ) were much more efficient TrxR1 inhibitors than APR-246 itself, indicating that MQ is the active compound responsible for TrxR1 enzyme inhibition. TrxR1 inhibited by MQ was still functional as a pro-oxidant NADPH oxidase. Knockdown of TrxR1 caused a partial and reproducible attenuation of APR-246-induced tumor cell death independently of p53 status. Cellular TrxR1 activity was also inhibited by APR-246 irrespective of p53 status. We show that APR-246 can directly affect cellular redox status via targeting of TrxR1. Our findings provide an explanation for the previously observed effects of APR-246 on tumor cells lacking mutant p53.

Anti-inflammatory and anti-proliferative effect of herbal medicines (APR) in RAW264.7 cells

CHOI, HAN-SEOK; SEO, HYE SOOK; KIM, SOON RE; CHOI, YOUN KYUNG; SHIN, YONG-CHEOL; KO, SEONG-GYU
Fonte: D.A. Spandidos Publicador: D.A. Spandidos
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
26.89%
The objective of the present study was to analyze the effect of a mixture of medicinal plants [Angelica gigas Nakai, Panax ginseng and Rhus verniciflua Stokes (APR)] on lipopolysaccharide (LPS)-induced inflammatory responses in the murine macrophage cell line RAW264.7. Cells were treated with APR and LPS at various concentrations and indicated times. WST assay, trypan blue assay and quantification of activated cells demonstrated that APR suppressed cell proliferation in a dose-dependent manner. APR induced G1 cell cycle arrest and inhibited the LPS-induced phosphorylation of protein kinase B (AKT), extracellular signal-regulated kinase (ERK), mitogen-activated protein kinase (p38) and necrosis factor κB (NF-κB). APR also suppressed nitric oxide synthase isoform (iNOS) and prostaglandin endoperoxide synthase 2 (Cox-2) messenger ribonucleic acid (mRNA) expression induced by LPS. Furthermore, APR decreased LPS-induced intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential, as well as induced PARP and caspase-3 cleavage, suggesting that APR causes apoptosis. In conclusion, the present study indicated that APR may be advantageous in treating inflammatory disease.

Study on the Usefulness of APR Scores from the Viewpoint of Proinflammatory Cytokines

Nakamura, T.; Hatanaka, D.; Yoshioka, T.; Yamada, S.; Goto, H.
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
26.89%
Background. Delayed diagnosis and treatment of newborn infection adversely impact outcomes. Clinical laboratory parameters have aimed to obtain the most correct and prompt diagnosis and treatment of this disease. This study simultaneously observed changes over time in APR as well as proinflammatory cytokines and anti-proinflammatory cytokines and aims to clarify usefulness of APR scores. Methods. We evaluated the usefulness of acute phase reactants (APR) in 46 newborns whose serum up to age 7 days had been stored, with comparison of three types (Group I: infection 15, Group F: fetal inflammatory response syndrome 17, and Group C: control 14) of APR-based scores, those of C-reactive protein (CRP), alpha1-acid glycoprotein (AGP), and haptoglobin (Hp), with proinflammatory cytokine levels. APR scores for CRP, AGP, and Hp and the levels of the proinflammatory cytokines IL-1β, IL-6, IL-8, IL-10, and TNFα were determined. Results. The cytokine levels started to increase from age 0 days and then decreased rapidly. The three APR scores, CRP, AG, and Hp, were elevated at age 0 days and then gradually decreased in infection (Group I) and fetal inflammatory response syndrome (Group F). The duration of antibiotic administration according to APR scores was significantly shorter in Group F than in Group I. Conclusion. This study demonstrated APR scores to be more useful for deciding whether antibiotics should be discontinued than proinflammatory cytokine levels.

Uma proposta para a inserção do tempo de uso dos equipamentos no aplicativo APR e estudos avaliativos de casos reais de PID´S

Gadenz, Fernando
Fonte: Universidade Federal de Uberlândia Publicador: Universidade Federal de Uberlândia
Tipo: Dissertação
Português
Relevância na Pesquisa
26.53%
Uma questão bastante controversa e que assume crescente importância no cenário nacional corresponde aos assuntos contemplados dentro da sigla PID (Pedidos de Indenização por Danos). Esta área, dentre outros aspectos, tem por objetivo oferecer respostas para as mais distintas solicitações de indenização por danos ocorridos em equipamentos instalados junto aos consumidores residenciais, comerciais e industriais. Com este foco, o tema em pauta tem por função primordial correlacionar eventuais danos em equipamentos com ocorrências manifestadas nas redes elétricas, emitindo, assim, um parecer final sobre o nexo causal entre os fenômenos e seus efeitos. Neste contexto, os procedimentos atuais seguem uma linha bastante empírica e, a busca por meios computacionais, fundamentados nos princípios da ciência e tecnologia, surge como uma alternativa importante, consistente e imparcial para a busca das almejadas respostas. É pois dentro deste cenário que encontra-se inserida a presente dissertação, a qual, somado a outras questões, contribui em duas direções: a primeira voltada para a proposição e incorporação, num programa já desenvolvido (APR), dos efeitos associados com o tempo de uso dos equipamentos, e a segunda encontra-se direcionada para a realização de estudos investigativos visando a validação do software. Esta última contribuição encontra-se alicerçada em 25 (vinte e cinco) casos reais de pedidos de ressarcimento ocorridos no âmbito do sistema de distribuição da concessionária Centrais Elétricas Matogrossenses S.A – CEMAT e na correlação entre os indicativos do programa e as ações tomadas pela empresa. ______________________________________________________________________________ ABSTRACT; A very controversial issue that assumes increasing importance in the national scenario is related to the well area known as Refunding Request for Damages. This subject...

What is the Benefit of Salt Reduction on Blood Pressure? Assessment of the Cochrane Review “Effect of longer-term modest salt reduction on blood pressure. He FJ, Li J, Macgregor GA. Cochrane Database Syst Rev. 2013 Apr 30;4:CD004937.; Qual é o Impacto da Redução da Ingestão de Sal na Pressão Arterial? Análise da Revisão Sistemática Cochrane “Effect of longer-term modest salt reduction on blood pressure. He FJ, Li J, Macgregor GA. Cochrane Database Syst Rev. 2013 Apr 30;4:CD004937.

Caldeira, Daniel; Laboratório de Farmacologia Clínica e Terapêutica. Faculdade Medicina da Universidade de Lisboa. Lisboa. Portugal.; Vaz-Carneiro, António; Centro de Estudos de Medicina Baseada na Evidência. Faculdade Medicina da Universidade de Lis
Fonte: Ordem dos Médicos Publicador: Ordem dos Médicos
Tipo: Artigo de Revista Científica Formato: application/pdf
Publicado em 31/10/2013 Português
Relevância na Pesquisa
26.65%
In the present article we evaluate and comment the systematic review from Cochrane Collaboration “Effect of longer-term modest salt reduction on blood pressure. Cochrane Database Syst Rev. 2013 Apr 30;4:CD004937”.Clinical Question: What is the impact of long-term (≥ 4 weeks) modest salt (sodium chloride) reduction on arterial blood pressure?Conclusions: This systematic review concluded that a moderate reduction in salt intake (mean reduction -4.4 g per day) resulted in statistically significant decreases in blood pressure (BP) that may be helpful in normotensive (-2.42 mmHg systolic BP; -1.00 mmHg diastolic BP) and hypertensive patients (-5.39 mmHg systolic BP; -2.82 mmHg diastolic BP). There were slight increases in physiological plasma renin activity, aldosterone and norepinephrine, without significant changes in lipid profile.; No presente artigo avaliamos e comentamos a Revisão Sistemática da Cochrane “Effect of longer-term modest salt reduction on blood pressure. Cochrane Database Syst Rev. 2013 Apr 30;4:CD004937”.Questão Clinica: Qual o impacto a longo prazo (≥ 4 semanas) da restrição moderada de ingestão de sal na pressão arterial.Conclusões: Esta revisão sistemática concluiu que a restrição moderada de ingestão de sal (redução média -4...

A comparison of the genetics of seedling and adult plant resistance to the spot form of net blotch (Pyrenophora teres f. maculata)

Williams, K.; Platz, G.; Barr, A.; Cheong, J.; Willsmore, K.; Cakir, M.; Wallwork, H.
Fonte: C S I R O Publishing Publicador: C S I R O Publishing
Tipo: Artigo de Revista Científica
Publicado em //2003 Português
Relevância na Pesquisa
26.79%
Spot form of net blotch (SFNB) (Pyrenophora teres f. maculata) is an economically damaging foliar disease of barley in many of the world's cereal-growing areas. The gene Rpt4 that confers seedling resistance to SFNB has been mapped on the long arm of chromosome 7H, but no genes for adult plant resistance (APR) have been identified. A lack of field resistance to SFNB in breeders' lines selected for Rpt4 led us to investigate the genetics of APR to this disease. Five doubled-haploid populations were phenotyped for seedling and adult plant reaction. Markers linked to Rpt4 explained a large part of the seedling variation, but little of the APR. In 2 mapped populations, major quantitative trait loci (QTLs) for APR distal to Rpt4 on chromosome 7H were identified. QTLs contributing to APR on chromosomes 4H or 5H were also identified in each population. Association of the 5H QTL with a gene for cereal cyst nematode resistance and the probable effect of this linkage on the historical development of cultivars with adult plant resistance to SFNB is discussed.; K. J. Williams , G. J. Platz, A. R. Barr, J. Cheong, K. Willsmore, M. Cakir and H. Wallwork

Estudos avaliativos do desempenho do aplicativo APR através de casos reais de consumidores da região metropolitana de Cuiabá

Quadrado Júnior, José Nelson
Fonte: Universidade Federal de Uberlândia Publicador: Universidade Federal de Uberlândia
Tipo: Dissertação
Português
Relevância na Pesquisa
26.73%
Nos últimos anos os pedidos de ressarcimento por danos em equipamentos eletroeletrônicos têm aumentado substancialmente, merecendo a atenção de todas as concessionárias de distribuição de energia elétrica, agências de regulação, consumidores e outros órgãos governamentais. Tais preocupações motivaram o tema central dessa dissertação, que tem como objetivo estabelecer os primeiros procedimentos voltados para o processo de validação de um programa computacional – APR, desenvolvido no âmbito da Universidade Federal de Uberlândia e destinado a emissão de pareceres sobre pedidos de ressarcimentos. A estratégia consiste em comparar os indicativos fornecidos pelo mencionado aplicativo com os pareceres oferecidos pelas concessionárias de energia elétrica através de seus métodos clássicos. Em consonância com o programa utilizado os procedimentos empregados para os trabalhos compreendem etapas atreladas com: a identificação do consumidor reclamante, modelagem do respectivo alimentador, reprodução do distúrbio ocorrido, definição e simulação dos equipamentos contemplados e, por fim, o estabelecimento da correlação entre os esforços dielétricos e térmicos impostos aos equipamentos e suas respectivas suportabilidades. Com tal estratégia em mente...

Risk-adjusting acute myocardial infarction mortality: are APR-DRGs the right tool?

Romano, P. S.; Chan, B. K.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /03/2000 Português
Relevância na Pesquisa
26.89%
OBJECTIVE: To determine if a widely used proprietary risk-adjustment system, APR-DRGs, misadjusts for severity of illness and misclassifies provider performance. DATA SOURCES: (1) Discharge abstracts for 116,174 noninstitutionalized adults with acute myocardial infarction (AMI) admitted to nonfederal California hospitals in 1991-1993; (2) inpatient medical records for a stratified probability sample of 974 patients with AMIs admitted to 30 California hospitals between July 31, 1990 and May 31, 1991. STUDY DESIGN: Using the 1991-1993 data set, we evaluated the predictive performance of APR-DRGs Version 12. Using the 1990/1991 validation sample, we assessed the effect of assigning APR-DRGs based on different sources of ICD-9-CM data. DATA COLLECTION/EXTRACTION METHODS: Trained, blinded coders reabstracted all ICD-9-CM diagnoses and procedures, and established the timing of each diagnosis. APR-DRG Risk of Mortality and Severity of Illness classes were assigned based on (1) all hospital-reported diagnoses, (2) all reabstracted diagnoses, and (3) reabstracted diagnoses present at admission. The outcome variables were 30-day mortality in the 1991-1993 data set and 30-day inpatient mortality in the 1990/1991 validation sample. PRINCIPAL FINDINGS: The APR-DRG Risk of Mortality class was a strong predictor of death (c = .831-.847)...

APR-246 overcomes resistance to cisplatin and doxorubicin in ovarian cancer cells

Mohell, N; Alfredsson, J; Fransson, Å; Uustalu, M; Byström, S; Gullbo, J; Hallberg, A; Bykov, V J N; Björklund, U; Wiman, K G
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
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Two main causes of platinum resistance are mutation in the tumor suppressor gene TP53 and drug-induced increase in intracellular glutathione concentration. Mutations in TP53 occur in about 50% of human tumors. APR-246 (PRIMA-1MET) is the first clinical-stage compound that reactivates mutant p53 and induces apoptosis. APR-246 is a prodrug that is converted to the active compound methylene quinuclidinone (MQ), a Michael acceptor that binds to cysteine residues in mutant p53 and restores its wild-type conformation. Here, we show that MQ also binds to cysteine in glutathione, thus decreasing intracellular free glutathione concentration. We also show that treatment with APR-246 completely restores the cisplatin and doxorubicin sensitivity to p53-mutant drug-resistant ovarian cancer cells. We propose that this unique ability of APR-246/MQ to bind to cysteines in both mutant p53 and glutathione has a key role in the resensitization as well as in the outstanding synergistic effects observed with APR-246 in combination with platinum compounds in ovarian cancer cell lines and primary cancer cells. However, MQ binding to cysteines in other targets, for example, thioredoxin reductase, may contribute as well. Strong synergy was also observed with the DNA-damaging drugs doxorubicin and gemcitabine...