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Propolis: Anti-Staphylococcus aureus activity and synergism with antimicrobial drugs

Fernandes Jr., Ary; Balestrin, Elaine Cristina; Betoni, Joyce Elaine Cristina; De Oliveira Orsi, Ricardo; De Souza Da Cunha, Maria de Lourdes Ribeiro; Montelli, Augusto Cezar
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 563-566
Português
Relevância na Pesquisa
55.85%
Propolis is a natural resinous substance collected by bees from tree exudates and secretions. Its antimicrobial activity has been investigated and inhibitory action on Staphylococcus aureus growth was evaluated The in vitro synergism between ethanolic extract of propolis (EEP) and antimicrobial drugs by two susceptibility tests (Kirby and Bauer and E-Test) on 25 S. aureus strains was evaluated Petri dishes with sub-inhibitory concentrations of EEP were incubated with 13 drugs using Kirby and Bauer method and synergism between EEP and five drugs [choramphenicol (CLO), gentamicin (GEN), netilmicin (NET), tetracycline (TET), and vancomycin (VAN)] was observed. Nine drugs were assayed by the E-test method and five of them exhibited a synergism [CLO, GEN, NET, TET, and clindamycin (CLI)]. The results demonstrated the synergism between EEP and antimicrobial drugs, especially those agents that interfere on bacterial protein synthesis.

Effects of anti-inflammatory agents on mucosal inflammation induced by infection with gram-negative bacteria.

Linder, H; Engberg, I; van Kooten, C; de Man, P; Svanborg-Edén, C
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /07/1990 Português
Relevância na Pesquisa
45.95%
Gram-negative bacterial infections of the urinary tract elicit a mucosal inflammatory response. Interleukin-6 is secreted into the urine, and polymorphonuclear leukocytes (PMNL) are recruited. In the present study we examined the effect of anti-inflammatory agents on these parameters and on bacterial clearance from the kidneys. Dexamethasone reduced interleukin-6 secretion, the PMNL response, and bacterial clearance. Diclofenac abolished the urinary interleukin-6 response but reduced the PMNL response and bacterial clearance only at the highest concentrations. Indomethacin drastically decreased bacterial clearance without the corresponding effect on interleukin-6 production or the PMNL response. The results demonstrate that the inhibition of inflammation impairs bacterial clearance from the kidneys. This is, however, not a direct function of inhibited interleukin-6 production or PMNL recruitment.

Repurposing Drugs in Oncology (ReDO)—clarithromycin as an anti-cancer agent

Van Nuffel, An MT; Sukhatme, Vidula; Pantziarka, Pan; Meheus, Lydie; Sukhatme, Vikas P; Bouche, Gauthier
Fonte: Cancer Intelligence Publicador: Cancer Intelligence
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
55.91%
Clarithromycin (CAM) is a well-known macrolide antibiotic available as a generic drug. CAM is traditionally used for many types of bacterial infections, treatment of Lyme disease and eradication of gastric infection with Helicobacter pylori. Extensive preclinical and clinical data demonstrate a potential role for CAM to treat various tumours in combination with conventional treatment. The mechanisms of action underlying the anti-tumour activity of CAM are multiple and include prolonged reduction of pro-inflammatory cytokines, autophagy inhibition, and anti-angiogenesis. Here, we present an overview of the current preclinical (in vitro and in vivo) and clinical evidence supporting the role of CAM in cancer. Overall these findings justify further research with CAM in many tumour types, with multiple myeloma, lymphoma, chronic myeloid leukaemia (CML), and lung cancer having the highest level of evidence. Finally, a series of proposals are being made to further investigate the use of CAM in clinical trials which offer the greatest prospect of clinical benefit to patients.

Development of a quantitative murine model of implant-associated osteomyelitis and its use to demonstrate the adverse effects of anti-resorptive drugs and efficacy of an immuno-dominant antigen vaccine on staphylococcus aureus infection of bone

Li, Dan ; Schwarz, Edward M.
Fonte: Universidade de Rochester Publicador: Universidade de Rochester
Tipo: Tese de Doutorado Formato: Number of Pages:xvi, 150 leaves
Português
Relevância na Pesquisa
46.09%
Thesis (Ph.D.)--University of Rochester. School of Medicine and Dentistry, Dept. of Biomedical Genetics, 2007.; Staphylococcus aureus, a common mammalian commensal bacterium, is the major cause of bone infection known as osteomyelitis (OM). This problem is mostly associated with the implantation of orthopedic devices. Although improvements in surgical technique and aggressive antibiotic prophylaxis have greatly decreased the infection rate following orthopedic implant surgery, OM appears to be on the rise from minimally invasive surgery. The significance of this resurgence is amplified by the fact that methicillin-resistant S. aureus (MRSA) has recently over-taken HIV as the most deadly pathogen in the United States. This indicates that there is a major need for the development of novel diagnostics, vaccines and therapeutics in this field. Although OM remains a serious problem in orthopedics, progress has been limited by the absence of an in vivo animal model that can quantify the bacterial load, metabolic activity of the bacteria over time, immunity and osteolysis. To overcome these obstacles, we first developed a murine model of implant-associated OM in which a stainless steel pin is coated with S. aureus and implanted transcortically through the tibial metaphysis. By using luxABCDE genes transformed S. aureus strain (Xen 29)...

Community acquired urinary tract infection: etiology and bacterial susceptibility

Dias Neto, José Anastácio; Martins, Antonio Carlos Pereira; Silva, Leonardo Dias Magalhães da; Tiraboschi, Ricardo Brianezi; Domingos, André Luis Alonso; Cologna, Adauto José; Paschoalin, Edson Luis; Tucci Júnior, Silvio
Fonte: Acta Cirurgica Brasileira Publicador: Acta Cirurgica Brasileira
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
55.82%
PURPOSE: Urinary tract infections (UTI) are one of the most common infectious diseases diagnosed. UTI account for a large proportion of antibacterial drug consumption and have large socio-economic impacts. Since the majority of the treatments begins or is done completely empirically, the knowledge of the organisms, their epidemiological characteristics and their antibacterial susceptibility that may vary with time is mandatory. OBJECTIVE: The aim of this study was to report the prevalence of uropathogens and their antibiotic susceptibility of the community acquired UTI diagnosed in our institution and to provide a national data. METHODS: We analyzed retrospectively the results of urine cultures of 402 patients that had community acquired urinary tract infection in the year of 2003. RESULTS: The mean age of the patients in this study was 45.34 ± 23.56 (SD) years. There were 242 (60.2%) females and 160 (39.8%) males. The most commonly isolated organism was Escherichia coli (58%). Klebsiella sp. (8.4%) and Enterococcus sp.(7.9%) were reported as the next most common organisms. Of all bacteria isolated from community acquired UTI, only 37% were sensitive to ampicillin, 51% to cefalothin and 52% to trimethoprim/sulfamethoxazole. The highest levels of susceptibility were to imipenem (96%)...

Atividade antimicrobiana dos extratos hidroalcoólicos dos frutos do Cerrado Genipa americana L., Dipteryx alata Vog. E Vitex cymosa Bert

Santos, Fabiola Brandão dos
Fonte: Universidade Federal de Mato Grosso do Sul Publicador: Universidade Federal de Mato Grosso do Sul
Tipo: Dissertação de Mestrado
Português
Relevância na Pesquisa
45.87%
A resistência microbiana é um sério problema de saúde pública que ocorre no mundo todo, os microrganismos adquirem mecanismos que os tornam resistentes aos mais diversos agentes antimicrobianos. Assim, buscam-se novas alternativas para o tratamento das infecções causadas por esses agentes. O cerrado brasileiro contém inúmeras espécies com compostos bioativos e potencial antimicrobiano, tais como, os frutos de Genipa americana L. (jenipapo), Dipteryx alata Vog. (baru) e Vitex cymosa Bert. (tarumã). A presente pesquisa teve como objetivo avaliar a atividade antimicrobiana dos extratos hidroalcoólicos dos frutos jenipapo, baru e tarumã frente aos microrganismos Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa e Candida albicans, através dos testes de difusão em disco e microdiluição. Os principais resultados obtidos em relação ao extrato de jenipapo mostraram halos de inibição frente a todos os microrganismos testados, com destaque para o extrato da polpa a 30% que obteve os maiores halos, em especial para o Staphylococcus aureus (6,5mm). Os extratos do baru apresentaram resultados satisfatórios nos testes de difusão em disco, sendo que a polpa obteve maiores halos de inibição para S. aureus e os extratos da casca a 20 e 30% foram melhores para E. coli. Os extratos de tarumã mostraram bons resultados...

Colistin: the re-emerging antibiotic for multidrug-resistant gram-negative bacterial infections

Li, J.; Nation, R.; Turnidge, J.; Milne, R.; Coulthard, K.; Rayner, C.; Paterson, D.
Fonte: The Lancet Publishing Group Publicador: The Lancet Publishing Group
Tipo: Artigo de Revista Científica
Publicado em //2006 Português
Relevância na Pesquisa
55.76%
Increasing multidrug resistance in Gram-negative bacteria, in particular Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae, presents a critical problem. Limited therapeutic options have forced infectious disease clinicians and microbiologists to reappraise the clinical application of colistin, a polymyxin antibiotic discovered more than 50 years ago. We summarise recent progress in understanding the complex chemistry, pharmacokinetics, and pharmacodynamics of colistin, the interplay between these three aspects, and their effect on the clinical use of this important antibiotic. Recent clinical findings are reviewed, focusing on evaluation of efficacy, emerging resistance, potential toxicities, and combination therapy. In the battle against rapidly emerging bacterial resistance we can no longer rely entirely on the discovery of new antibiotics; we must also pursue rational approaches to the use of older antibiotics such as colistin.; Jian Li, Roger L Nation, John D Turnidge, Robert W Milne, Kingsley Coulthard, Craig R Rayner, David L Paterson

Evaluation of colistin as an agent against multi-resistant Gram-negative bacteria

Li, J.; Nation, R.; Milne, R.; Turnidge, J.; Coulthard, K.
Fonte: Elsevier Science BV Publicador: Elsevier Science BV
Tipo: Artigo de Revista Científica
Publicado em //2005 Português
Relevância na Pesquisa
55.93%
Infections caused by multi-resistant Gram-negative bacteria, particularly Pseudomonas aeruginosa, are increasing worldwide. In patients with cystic fibrosis (CF), resistance in P. aeruginosa to numerous anti-pseudomonal agents is becoming common. The absence since 1995, of new substances active against resistant Gram-negative bacteria, has caused increasing concern. Colistin, an old antibiotic also known as polymyxin E, has attracted more interest recently because of its significant activity against multi-resistant P. aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae, and the low resistance rates to it. Because its use as an anti-pseudomonal agent was displaced by the potentially less toxic aminoglycosides in 1970s, our knowledge of this drug is limited. However, there has been a significant recent increase in the data gathered on colistin, focussing on its chemistry, antibacterial activity, mechanism of action and resistance, pharmacokinetics, pharmacodynamics and new clinical application. It is likely that colistin will be an important antimicrobial option against multi-resistant Gram-negative bacteria, for some years to come.

Doripenem activity tested against a global collection of Enterobacteriaceae, including isolates resistant to other extended-spectrum agents

Mendes, R.; Rhomberg, P.; Bell, J.; Turnidge, J.; Sader, H.
Fonte: Elsevier Science Inc Publicador: Elsevier Science Inc
Tipo: Artigo de Revista Científica
Publicado em //2009 Português
Relevância na Pesquisa
55.74%
The emergence and rapid dissemination of extended-spectrum beta-lactamase (ESBL)-producing isolates among Enterobacteriaceae coupled with increasing prevalence of stably derepressed and plasmid-borne AmpC producers have rendered broad-spectrum cephalosporins and beta-lactam/beta-lactamase inhibitor combinations less effective. This scenario has required the use of carbapenems for treatment of infections caused by such organisms. In this study, the in vitro activities of doripenem and comparator agents against Enterobacteriaceae, including ESBL- and AmpC-producing strains, were evaluated. A total of 36 614 isolates collected from more than 60 medical centers (2000-2007) were included and tested for susceptibility using reference methods and interpretive criteria, except for doripenem (product package insert). Overall, doripenem inhibited 98.7% of all Enterobacteriaceae tested at

Review article: nitric oxide from dysbiotic bacterial respiration of nitrate in the pathogenesis and as a target for therapy of ulcerative colitis

Roediger, W.
Fonte: Blackwell Publishing Ltd Publicador: Blackwell Publishing Ltd
Tipo: Artigo de Revista Científica
Publicado em //2008 Português
Relevância na Pesquisa
55.76%
BACKGROUND: Factors initiating human ulcerative colitis (UC) are unknown. Dysbiosis of bacteria has been hypothesized to initiate UC but, to date, neither the nature of the dysbiosis nor mucosal breakdown has been explained. AIM: To assess whether a dysbiosis of anaerobic nitrate respiration could explain the microscopic, biochemical and functional changes observed in colonocytes of UC. METHODS: Published results in the gastroenterological, biochemical and microbiological literature were reviewed concerning colonocytes, nitrate respiration and nitric oxide in the colon in health and UC. A best-fit explanation of results was made regarding the pathogenesis and new treatments of UC. RESULTS: Anaerobic nitrate respiration yields nitrite, nitric oxide (NO) and nitrous oxide. Colonic bacteria produce NO and UC in remission has a higher lumenal NO level than control cases. NO with sulphide, but not NO alone, impairs beta-oxidation, lipid and protein synthesis explaining the membrane, tight junctional and ion channel changes observed in colonocytes of UC. The observations complement therapeutic mechanisms of those probiotics, prebiotics and antibiotics useful in treating UC. CONCLUSIONS: The prolonged production of bacterial NO with sulphide can explain the initiation and barrier breakdown...

The efficacy of topical agents in the treatment of bacterial biofilms: an in vivo sheep study and an in vitro study.

Le, Tong Ba
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2010 Português
Relevância na Pesquisa
46.1%
Introduction: Recent evidence has demonstrated the presence of bacterial biofilms on the mucosa of patients with Chronic Rhinosinusitis (CRS), suggesting their role in the pathogenesis of the condition. This thesis contains two separate studies. The studies investigated novel topical therapies by using previously established in vitro and in vivo biofilm growth and detection methods. In the first study, several different proposed anti-biofilm agents were evaluated in a sheep biofilm model, each with varying degrees of immediate and short-term success against Staphylococcus aureus biofilms. A second study was conducted to determine the in vitro anti-bacterial and anti-biofilm properties of Chitosan/Dextran (CD) gel, a novel chitosan-based product with remarkable mucosal healing and haemostatic properties. Methods: Three alternative anti-biofilm treatments: Mupirocin, CAZS (Citric Acid Zwitterionic Acid) and Gallium Nitrate were evaluated in a prospective randomized controlled single-blinded trial using a previously established sheep biofilm model of CRS. The sheep mucosal samples were analyzed for presence of S. aureus biofilms using BacLight staining and CLSM, and the degree of biofilm involvement was determined using FISH (Fluorescence In-Situ Hybridization). The MIC/MBC values for CD gel and its constituents were determined by macro-dilution methods described by Jorgensen et al.[1]. Established in vitro biofilms grown from common CRS pathogens (ATCC strains and clinical isolates) were subjected to treatment by CD gel and its components (chitosan and dextran). A 96-well micro-titre crystal-violet staining method described by O’Toole and Kolter [2] was used to determine the anti-biofilm profile of CD gel against several bacterial strains with known biofilm-forming capacity. Results: Following 8 days of inoculation with S. aureus...

Chemical inhibition of bacterial protein tyrosine phosphatase suppresses capsule production

Standish, A.; Salim, A.; Zhang, H.; Capon, R.; Morona, R.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em //2012 Português
Relevância na Pesquisa
55.84%
Capsule polysaccharide is a major virulence factor for a wide range of bacterial pathogens, including Streptococcus pneumoniae. The biosynthesis of Wzy-dependent capsules in both Gram-negative and –positive bacteria is regulated by a system involving a protein tyrosine phosphatase (PTP) and a protein tyrosine kinase. However, how the system functions is still controversial. In Streptococcus pneumoniae, a major human pathogen, the system is present in all but 2 of the 93 serotypes found to date. In order to study this regulation further, we performed a screen to find inhibitors of the phosphatase, CpsB. This led to the observation that a recently discovered marine sponge metabolite, fascioquinol E, inhibited CpsB phosphatase activity both in vitro and in vivo at concentrations that did not affect the growth of the bacteria. This inhibition resulted in decreased capsule synthesis in D39 and Type 1 S. pneumoniae. Furthermore, concentrations of Fascioquinol E that inhibited capsule also lead to increased attachment of pneumococci to a macrophage cell line, suggesting that this compound would inhibit the virulence of the pathogen. Interestingly, this compound also inhibited the phosphatase activity of the structurally unrelated Gram-negative PTP...

Regional resistance surveillance program results for 12 Asia-Pacific nations (2011)

Mendes, R.; Mendoza, M.; Banga Singh, K.; Castanheira, M.; Bell, J.; Turnidge, J.; Lin, S.; Jones, R.
Fonte: Amer Soc Microbiology Publicador: Amer Soc Microbiology
Tipo: Artigo de Revista Científica
Publicado em //2013 Português
Relevância na Pesquisa
45.92%
The Regional Resistance Surveillance program monitored susceptibility rates and developing resistance by geographic region, including 12 Asia-Pacific (APAC) countries. Reference broth microdilution methods for susceptibility/interpretations were applied, processing 5,053 strains. Among Staphylococcus aureus isolates (37% methicillin-resistant S. aureus [MRSA], highest in South Korea [73%]), linezolid (LZD), tigecycline (TIG), and vancomycin were 100% active, but 33 and 34% of strains were levofloxacin (LEV) or macrolide resistant, respectively. Streptococcus pneumoniae was most resistant to β-lactams and macrolides (45%) but was LZD, LEV, and TIG susceptible (>98%). Extended-spectrum β-lactamase (ESBL) phenotype rates in Escherichia coli and Klebsiella spp. were 48 and 47%, respectively, and were highest in Taiwan, at 75 to 91%. The best anti-ESBL-phenotype agents were amikacin (81 to 96% susceptible), colistin (COL; >98%), TIG (>98%), and carbapenems (81 to 97%). Pseudomonas aeruginosa showed ≥20% resistance to all drugs except COL (99% susceptible). In conclusion, endemic evolving antimicrobial resistances in APAC nations show compromised roles for many commonly used antimicrobials.; Rodrigo E. Mendes, Myrna Mendoza, Kirnpal K. Banga Singh...

Platforms for controlled release of antibacterial agents facilitated by plasma polymerization

Vasilev, K.; Simovic, S.; Losic, D.; Griesser, H.; Griesser, S.; Anselme, K.; Ploux, L.
Fonte: IEEE; USA Publicador: IEEE; USA
Tipo: Conference paper
Publicado em //2010 Português
Relevância na Pesquisa
55.82%
Bacterial infections present an enormous problem causing human suffering and cost burdens to the healthcare systems worldwide. Herein we present several versatile strategies for controlled release of antibacterial agents which include silver ions as well as traditional antibiotics. At the heart of these release platforms is a thin film deposited by plasma polymerization. The use of plasma polymerization makes these strategies applicable to the surface of many types of medical devices since the technique for deposition of a polymer film from plasma in practically substrate independent.; Krasimir Vasilev, Spomenka Simovic, Dusan Losic, Hans J. Griesser, Stefani Griesser, Karine Anselme and Lydie Ploux

Guidelines for the diagnosis and antimicrobial therapy of canine superficial bacterial folliculitis (Antimicrobial Guidelines Working Group of the International Society for Companion Animal Infectious Diseases)

Hillier, A.; Lloyd, D.H.; Weese, J.S.; Blondeau, J.M.; Boothe, D.; Breitschwerdt, E.; Guardabassi, L.; Papich, M.G.; Rankin, S.; Turnidge, J.D.; Sykes, J.E.
Fonte: Wiley Publicador: Wiley
Tipo: Artigo de Revista Científica
Publicado em //2014 Português
Relevância na Pesquisa
55.84%
BACKGROUND: Superficial bacterial folliculitis (SBF) is usually caused by Staphylococcus pseudintermedius and routinely treated with systemic antimicrobial agents. Infection is a consequence of reduced immunity associated with alterations of the skin barrier and underlying diseases that may be difficult to diagnose and resolve; thus, SBF is frequently recurrent and repeated treatment is necessary. The emergence of multiresistant bacteria, particularly meticillin-resistant S. pseudintermedius (MRSP), has focused attention on the need for optimal management of SBF. OBJECTIVES: Provision of an internationally available resource guiding practitioners in the diagnosis, treatment and prevention of SBF. DEVELOPMENT OF THE GUIDELINES: The guidelines were developed by the Antimicrobial Guidelines Working Group of the International Society for Companion Animal Infectious Diseases, with consultation and advice from diplomates of the American and European Colleges of Veterinary Dermatology. They describe optimal methods for the diagnosis and management of SBF, including isolation of the causative organism, antimicrobial susceptibility testing, selection of antimicrobial drugs, therapeutic protocols and advice on infection control. Guidance is given for topical and systemic modalities...

The inhibitory activity of synthetic compounds and ions against transporters of multi-drug resistant bacteria

Machado, Lisa Santos
Fonte: Faculdade de Ciências Médicas. Universidade Nova de Lisboa Publicador: Faculdade de Ciências Médicas. Universidade Nova de Lisboa
Tipo: Dissertação de Mestrado
Publicado em //2011 Português
Relevância na Pesquisa
45.97%
RESUMO: Sessenta e três derivados de hidantoína foram utilizados para avaliar possíveis efeitos de modulação na actividade das bombas de efluxo (BE) na Salmonella NCTC 13349 utilizando um método fluorimétrico semi-automático. Nenhum dos compostos apresentaram actividade anti-bacteriana até concentrações de 240 mg/L. Entre todos os compostos, SZ-7 demonstrou possuir propriedades de modulação de effluxo na presença de glucose. Para testar esta actividade, estirpes de Salmonella resistentes à ciprofloxacina, induzidas a elevados níveis de resistência com sobre-expressão de BE, foram expostas ao SZ-7. Este derivado afectou a susceptibilidade das estirpes à ciprofloxacina. Uma vez que os 63 compostos estudados apresentaram pouco efeito inibitório /cumulativo, apesar de serem conhecidos pelos seus efeitos moduladores de BE-dependentes de iões em eucariotas, foi questionado o papel dos iões na regulação de BE bacterianas, que poderão influenciar a eficácia de novos compostos. Para este estudo, utilizamos a Escherichia coli AG100 como modelo, devido ao extenso conhecimento no que respeita a estrutura e actividade das BE. Devido à importância de iões de cálcio (Ca2+) nos canais de transporte membranar e na actividade de ATPases...

Tri- and tetra-nuclear polypyridyl ruthenium(ii) complexes as antimicrobial agents

Gorle, A.K.; Feterl, M.; Warner, J.M.; Wallace, L.; Keene, F.R.; Collins, J.G.
Fonte: Royal Society of Chemistry Publicador: Royal Society of Chemistry
Tipo: Artigo de Revista Científica
Publicado em //2014 Português
Relevância na Pesquisa
55.74%
A series of inert tri- and tetra-nuclear polypyridylruthenium(II) complexes that are linked by the bis[4(4'-methyl-2,2'-bipyridyl)]-1,n-alkane ligand ("bb(n)" for n = 10, 12 and 16) have been synthesised and their potential as antimicrobial agents examined. Due to the modular nature of the synthesis of the oligonuclear complexes, it was possible to make both linear and non-linear tetranuclear ruthenium species. The minimum inhibitory concentrations (MIC) of the ruthenium(II) complexes were determined against four strains of bacteria--Gram positive Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA), and Gram negative Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa). In order to gain an understanding of the relative antimicrobial activities, the cellular uptake and water-octanol partition coefficients (log P) were determined for a selection of the ruthenium complexes. Although the trinuclear complexes were the most lipophilic based upon log P values and showed the greatest cellular uptake, the linear tetranuclear complexes were generally more active, with MIC values <1 μM against the Gram positive bacteria. Similarly, although the non-linear tetranuclear complexes were slightly more lipophilic and were taken up to a greater extent by the bacteria...

Estudo do perfil de resistência de bactérias Gram-negativas em infecções urinárias de origem comunitária : influência da legislação atuante no controle de venda de antimicrobianos; Gram-negative bacterial resistance in community acquired urinary tract infections : influence of an active control law for the sale of antimibrobials

Karen Prado Herzer Mattos
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 11/12/2014 Português
Relevância na Pesquisa
45.88%
As Infecções de Trato Urinário (ITUs) são definidas como colonizações microbianas com invasão tecidual de qualquer parte do trato urinário, desde a uretra até os rins, considerada a doença infecciosa extra intestinal de origem comunitária mais comum em todo mundo. As ITUs em sua maioria são causadas por bactérias Gram-negativas, sendo a Escherichia coli o micro-organismo invasor mais comum, isolado em cerca de 80% a 90% das infecções agudas de origem comunitária. Neste início de terceiro milênio, a resistência bacteriana é um dos desafios globais de saúde pública a ser enfrentado e sabe-se que, a intensidade de exposição ao antimicrobiano é um importante parâmetro relacionado à seleção e à manutenção de bactérias resistentes. Em 2010 o Brasil vivia uma situação na qual estavam sendo observados vários focos de infecções hospitalares causadas por micro-organismos multirresistentes como a Klebsiella pneumoniae produtoras de carbapenemase (KPC). Em função do preocupante cenário, em 2010 foi implantada a Resolução da Diretoria Colegiada (RDC) nº 44 da Agência Nacional de Vigilância Sanitária (ANVISA) que possui, dentre outros objetivos, a diminuição da resistência bacteriana aos antimicrobianos. Objetivo: Estudar o perfil de resistência de bactérias Gram-negativas relacionadas às ITUs de origem comunitária e analisar a influência da legislação atuante no controle de venda de antimicrobianos. Métodos: População de pacientes de demanda espontânea aos hospitais da Universidade Estadual de Campinas entre 2009 e 2013 com hipótese-diagnóstica de ITU de origem comunitária...

Susceptibilidad genética para el desarrollo de la sepsis bacteriana grave y choque séptico; Genetic susceptibility to develop of severe bacterial sepsis and septic shock; A predisposição genética para o desenvolvimento de sepsis bacteriana grave e choque séptico

Chávez, Mónica; Vallejo Prado, Diana Estefanía
Fonte: Universidade do Rosário Publicador: Universidade do Rosário
Tipo: Artigo de Revista Científica Formato: application/pdf
Publicado em 30/04/2013 Português
Relevância na Pesquisa
55.78%
Objetivo: presentar el estado del arte de las investigaciones que, hasta el momento, relacionan el polimorfismo genético del paciente con la evolución de la sepsis, como herramienta diagnóstica y un nuevo enfoque terapéutico de esta condición. Los conceptos actuales basados en investigaciones sostienen que el polimorfismo genético del individuo es relevante en la evolución de la enfermedad y en la respuesta efectiva al tratamiento del paciente en estado crítico, en especial con sepsis bacteriana y choque séptico. Materiales y métodos: se revisó literatura indexada que relaciona los factores genéticos con la evolución de algunas enfermedades del paciente en estado crítico. Resultados: las características particulares de la enfermedad estarían influenciadas por el acervo genético del paciente, condicionando en gran medida la respuesta patofisiológica. Se ha evidenciado la susceptibilidad genética de algunos individuos a desarrollar infección; estos individuos con un tratamiento similar no evolucionan de igual forma, desencadenándose una sepsis bacteriana grave y choque séptico. El polimorfismo en los genes que codifican por el factor de necrosis tumoral -α (TNF-α) las interlucinas- 1 (IL-1), IL-6, IL-10, el factor soluble CD-14...

The inoculum effect and band-pass bacterial response to periodic antibiotic treatment.

Tan, C; Smith, RP; Srimani, JK; Riccione, KA; Prasada, S; Kuehn, M; You, L
Fonte: Universidade Duke Publicador: Universidade Duke
Tipo: Artigo de Revista Científica Formato: 617 - ?
Publicado em //2012 Português
Relevância na Pesquisa
55.8%
The inoculum effect (IE) refers to the decreasing efficacy of an antibiotic with increasing bacterial density. It represents a unique strategy of antibiotic tolerance and it can complicate design of effective antibiotic treatment of bacterial infections. To gain insight into this phenomenon, we have analyzed responses of a lab strain of Escherichia coli to antibiotics that target the ribosome. We show that the IE can be explained by bistable inhibition of bacterial growth. A critical requirement for this bistability is sufficiently fast degradation of ribosomes, which can result from antibiotic-induced heat-shock response. Furthermore, antibiotics that elicit the IE can lead to 'band-pass' response of bacterial growth to periodic antibiotic treatment: the treatment efficacy drastically diminishes at intermediate frequencies of treatment. Our proposed mechanism for the IE may be generally applicable to other bacterial species treated with antibiotics targeting the ribosomes.