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Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media

OLIVEIRA, Angela de; FONSECA, Adivaldo Henrique; COSTA, Catia Marques da; MANTOVANI, Elenice; YOSHINARI, Natalino Hajime
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
65.99%
The aim of the present paper was to evaluate cyst formation and growth parameters of Borrelia garinii in a range of media differing in formulation and cost. A qualitative assessment of morphology and motility of B. garinii was conducted. All media were prepared aseptically and used in test tubes or Petri dishes. For each medium, the initial spirochete concentration was standardized to 10³ spirochets/mL. The following culture media were suitable to grow B. garinii: Barbour-Stoenner-Kelly, brain heart infusion and PMR. Growth was minimal at six weeks post-inoculation and maximum spirochete density was observed between 9-12 weeks. Often, the cultures developed cysts of different sizes, isolated or in groups, with a spiraled portion of variable sizes, mainly in unfavorable culture media. Brazilian Lyme disease-like illness, also known as Baggio-Yoshinari syndrome (BYS), is a new and interesting emerging tick-borne disease, caused by Borrelia burgdorferi sensu lato spirochetes, only during its cystic forms. It has been assumed that the peculiar clinical and laboratory features of BYS are consequential to the absence of a human sucker Ixodes ricinus complex tick at risk areas in Brazil, supporting the concept that the borrelia phenotypic expression pattern is modified as it is transmitted through the host.; CNPq; (FAPERJ) Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro

Evaluation of culture media for enumeration of Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium animalis in the presence of Lactobacillus delbrueckii subsp bulgaricus and Streptococcus thermophilus

LIMA, Katia Gianni de Carvalho; KRUGER, Monika Francisca; BEHRENS, Jorge; DESTRO, Maria Teresa; LANDGRAF, Mariza; FRANCO, Bernadette Dora Gombossy de Melo
Fonte: ELSEVIER SCIENCE BV Publicador: ELSEVIER SCIENCE BV
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
66.02%
The study compared the growth capability of probiotic (Lactobacillus acidophilus La05, Lactobacillus casei Lc01 and Bifidobacterium animalis Bb12) and non-probiotic (Lactobacillus delbrueckii subsp bulgaricus and Streptococcus thermophilus) cultures on twenty-one culture media grouped according to selectivity: nonselective agars, selective agars without antibiotics and MRS agars containing different combinations of lithium chloride, cystein, bile salts and antibiotics. Four of these media were selected for quantitative enumeration of L acidophilus La05, L casei Lc01, and B. animalis Bb12. The best culture media and incubation conditions for enumeration of the probiotic cultures were: B. animalis: MRS agar with dicloxacillin, 37 degrees C or 42 degrees C, anaerobiosis; L acidophilus: MRS agar with bile salts, 37 degrees C or 42 degrees C, aerobiosis; L casei: MRS agar with lithium chloride and sodium propionate, 37 degrees C or 42 degrees C, aerobiosis or anaerobiosis. Plating on MRS with glucose replaced by maltose, 37 degrees C or 42 degrees C, anaerobiosis, will distinguish probiotic from non-probiotic cultures. For enumeration of each probiotic in a mixed culture, the following media and incubation conditions were recommended: B. animalis: 4ABC-MRS...

Correlation between mastitis occurrence and the count of microorganisms in bulk raw milk of bovine dairy herds in four selective culture media

SOUTO, Luis I. M.; MINAGAWA, Clarice Y.; TELLES, Evelise O.; GARBUGLIO, Marcio A.; AMAKU, Marcos; MELVILLE, Priscilla A.; DIAS, Ricardo A.; SAKATA, Sonia T.; BENITES, Nilson R.
Fonte: CAMBRIDGE UNIV PRESS Publicador: CAMBRIDGE UNIV PRESS
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
66.06%
Milk is the normal secretion of the mammary gland, practically free of colostrum and obtained by the complete milking of oil(? or more healthy animals. Mastitis is all inflammatory process of the mammary gland and it may cause alterations in the milk. The present work aimed to verify whether it is possible, by means of the counts of microorganism in the bulk raw milk ill four selective culture media, to establish a correlation with the occurrence of mastitis and therefore, to monitor this disease in bovine dairy herds. The following selective culture media were Used: KF Streptococcus Agar, Edwards Agar, Baird-Parker Agar, Blood Agar plus potassium tellurite. Spearman`s correlation coefficient was calculated in order to compare the Occurrence of mastitis (percentage) in each herd with respective selective culture media counts of microorganisms in bulk raw milk. Thirty-six possibilities were analysed (Tamis and CMT-positive rates were compared with the log-transformed count in four selective Culture media) and there was a negative correlation between Tamis 3 and the Baird-Parker Agar plate count. The total results of microbiological tests showed that there were three correlations of the counts in selective culture media. Fifty-two possibilities were analysed and there was a negative correlation between no-bacteria I-growth mastitis rates and log(10) of KF Streptoccocus Agar plate Count and there were two positive correlations between coagulase-positive staphylococci and log(10) of Baird-Parker Agar plate count and Blood Agar plus potassium tellurite plate count.; National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPQ); State of Sao Paulo Research Fundation (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP)[ndegrees 141536/2002-0 e]; State of Sao Paulo Research Fundation (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP)[ndegrees 03/04785-0]

Crescimento e esporulação de Alternaria dauci e A. solani em meio de cultura; Growth and sporulation of Alternaria dauci e Alternaria solani in culture media

Pulz, Pablo
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 16/03/2007 Português
Relevância na Pesquisa
65.99%
Alternaria dauci e A. solani são duas espécies de fungos fitopatogênicos reconhecidamente difíceis de esporular em meio de cultura. Isto dificulta as inoculações artificiais e, conseqüentemente, prejudica o processo de seleção de genótipos de cenoura e tomate resistentes às doenças causadas por estes fungos. Este trabalho teve o objetivo de verificar a influência de alguns fatores, aplicados na incubação, sobre o crescimento micelial e esporulação das duas espécies fúngicas. Diferentes meios de cultura (BDA, aveia e V8), temperatura (15, 20, 25, 30 e 35 °C), comprimentos de onda da luz usada na incubação (amarela, azul, branca, NUV, verde e vermelha), tipos de estresse aplicado à colônia (raspagem, UV, irradiação de microondas e temperatura de 100 °C) e fotoperíodos (luz / escuro, respectivamente, de 24 h / 0 h, 22 h / 2 h, 17 h / 7 h, 12 h / 12 h, 7 h / 17 h, 2 h / 22 e 0 h / 24 h) foram testados. Após a determinação dos melhores fatores, o método desenvolvido neste trabalho foi comparado ao método tradicionalmente utilizado (BDA, 25 °C, 12 h luz branca / 12 h escuro e raspagem da colônia), utilizando diversos isolados de ambas as espécies. Os resultados indicaram o meio V8-ágar e a temperatura de 25 °C como os mais favoráveis ao crescimento e esporulação. Os diferentes comprimentos de onda utilizados tiveram influência marcante na esporulação...

Ocorrência de Salmonella spp em amostras de carcaças e miúdos de frango obtidas em uma feira e um mercado municipal na zona oeste da cidade de São Paulo: análise crítica entre a técnica convencional em meios de cultivo e reação em cadeia pela polimerase - PCR; Occurence of Salmonella spp in whole broiler carcasses and organ samples obtained at a commercial retail market in the western area of the city of São Paulo: a critical analysis of traditional culture media growth technique and polymerase chain reaction PCR

Maldonado, Alessandra Grangel
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 02/07/2008 Português
Relevância na Pesquisa
65.89%
O presente estudo teve por objetivos estudar a ocorrência de Salmonella spp em amostras de carcaças e miúdos de frango obtidas em uma feira e um mercado municipal da zona oeste da cidade de São Paulo e fazer uma análise crítica entre a técnica convencional em meios de cultivo e reação em cadeia pela polimerase PCR. Foram utilizadas 63 carcaças de frangos e 63 conjuntos de miúdos, obtidos no período de novembro de 2006 a maio de 2007. Pesquisou-se salmonela nas amostras pela técnica convencional em meios de cultivo e pela PCR. Pela técnica convencional identificou-se Salmonella em (6/63) das amostras de carcaça e (5/63) das amostras de miúdos e, pela PCR obteve-se (20/63) das amostras de miúdos e (28/63) das amostras de carcaça. As estirpes sorotipificadas como Salmonella oriundas das amostras de carcaças foram identificadas, como S. Senftenberg; S. Kentucky; S. Enteritidis; S. Montevideo; S. Infantis, S. enterica subsp enterica (0: 6.7). S. Enteritidis; S. enterica subsp enterica; S. Infantis. As estirpes provenientes das amostras de miúdos foram identificadas como S. Enteritidis; S. enterica subsp enterica; S. Infantis. As (18/21) estirpes identificadas como Salmonella pelo estudo apresentaram resistência aos antimicrobianos testados. O método convencional é indispensável quando se necessita a obtenção da estirpe para estudos de outras naturezas...

Comparison of different culture media for mycological evaluation of commercial pet food; Comparação de diferentes meios de cultura para a avaliação micológica de rações pet

Copetti, Marina Venturini; Santúrio, Jânio Morais; Cavalheiro, Ayrton; Alves, Sydney Hartz; Ferreiro, Laerte
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
Relevância na Pesquisa
66.03%
A seleção de um determinado meio de cultura para análise micológica de alimentos é de extrema importância para a garantia e segurança de uma análise, pois ao mesmo tempo que é desejável que este impeça o desenvolvimento de bactérias, ele deve permitir uma excelente recuperação das espécies fúngicas presentes no alimento, de modo que o resultado reflita a real ecologia microbiana no produto. Neste trabalho foi comparada a eficácia de três meios para isolamento e enumeração de fungos (ágar batata glicose, ágar dicloran rosa de bengala e cloranfenicol e ágar dicloran glicerol 18%) a partir de 54 amostras de rações comerciais pet (34 para cães e 20 para gatos). Foi verificada a presença de fungos em 74% das amostras e 23 gêneros foram isolados e identificados. Aspergillus sp. e Aspergillus niger foram, respectivamente, o gênero e espécie mais frequentemente isolados, independentemente do meio de cultura utilizados para proceder a análise O ágar dicloran glicerol 18% foi o meio que apresentou os melhores resultados, quando considerados tanto a quantidade quanto a variedade de fungos isolados. Comparando os resultados obtidos nos diferentes meios, foi observado que os fungos isolados podem variar de acordo com o meio de cultura selecionado. O gênero Eurotium foi o que apresentou a maior diferença de ocorrência entre os meios avaliados para enumeração de fungos neste estudo. Portanto...

A influência de diferentes meios de cultura na geração de células dendríticas para o tratamento imunoterápico de pacientes com leucemia mieloide aguda; The influence of different culture media in generation of dendritic cells for immunotherapeutic treatment of acute myeloid leukemia patients

Gisele da Silva Simoneti
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 04/01/2013 Português
Relevância na Pesquisa
65.97%
Células dendríticas (DCs) são as principais células apresentadoras de antígeno do sistema imune, capazes de estimular o linfócito T a iniciar resposta imune especifica. Vacinas de DCs vêm sendo utilizadas como forma de tratamento imunoterápico adjuvante para várias neoplasias. Protocolos para geração dessas células têm sido desenvolvidos e o método ideal de produção para uso clínico ainda necessita ser definido. É fundamental a definição de protocolos e reagentes que ofereçam, a partir de células mononucleares do sangue periférico, células dendríticas seguras e funcionais para uso clínico. A suplementação de meios de cultura com soro de origem animal e humano leva á riscos de xenosensibilização e transmissão de doenças. O uso do soro autólogo parece oferecer menos riscos ao paciente, porém a presença de fatores imunossupressores nesse soro poderia interferir na qualidade das DCs produzidas. Vários tipos de meios livres de soro, baseados nas boas práticas de produção - "good manufacture practice" (GMP), têm sido utilizados recentemente e parecem ser uma opção viável. O objetivo desse estudo foi avaliar os resultados da diferenciação, maturação e funcionalidade de DCs de pacientes com LMA...

Different culture media containing methyldopa for melanin production by Cryptococcus species

Menezes,Ralciane de Paula; Penatti,Mário Paulo Amante; Pedroso,Reginaldo dos Santos
Fonte: Sociedade Brasileira de Medicina Tropical - SBMT Publicador: Sociedade Brasileira de Medicina Tropical - SBMT
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2011 Português
Relevância na Pesquisa
65.98%
INTRODUCTION: Melanin production by species of Cryptococcus is widely used to characterize C. neoformans complex in mycology laboratories. This study aims to test the efficacy of methyldopa from pharmaceutical tablet as a substrate for melanin production, to compare the production of melanin using different agar base added with methyldopa, and to compare the melanin produced in those media with that produced in Niger seed agar and sunflower seed agar by C. neoformans, C. laurentii, and C. albidus. Two isolates of each species, C. neoformans, C. laurentii, and C. albidus, and one of Candida albicans were used to experimentally detect conditions for melanin production. METHODS: The following media were tested: Mueller-Hinton agar (MHA), brain and heart infusion agar (BHIA), blood agar base (BAB), and minimal medium agar (MMA), all added with methyldopa, and the media Niger seed agar (NSA) and sunflower seed agar (SSA). RESULTS: All isolates grew in most of the culture media after 24h. Strains planted on media BAB and BHIA showed growth only after 48h. All isolates produced melanin in MMA, MHA, SSA, and NSA media. CONCLUSIONS: Methyldopa in the form pharmaceutical tablet can be used as a substrate for melanin production by Cryptococcus species; minimal medium plus methyldopa was more efficient than the BAB...

Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media

Oliveira,Angela de; Fonseca,Adivaldo Henrique; Costa,Catia Marques da; Mantovani,Elenice; Yoshinari,Natalino Hajime
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2010 Português
Relevância na Pesquisa
65.99%
The aim of the present paper was to evaluate cyst formation and growth parameters of Borrelia garinii in a range of media differing in formulation and cost. A qualitative assessment of morphology and motility of B. garinii was conducted. All media were prepared aseptically and used in test tubes or Petri dishes. For each medium, the initial spirochete concentration was standardized to 10³ spirochets/mL. The following culture media were suitable to grow B. garinii: Barbour-Stoenner-Kelly, brain heart infusion and PMR. Growth was minimal at six weeks post-inoculation and maximum spirochete density was observed between 9-12 weeks. Often, the cultures developed cysts of different sizes, isolated or in groups, with a spiraled portion of variable sizes, mainly in unfavorable culture media. Brazilian Lyme disease-like illness, also known as Baggio-Yoshinari syndrome (BYS), is a new and interesting emerging tick-borne disease, caused by Borrelia burgdorferi sensu lato spirochetes, only during its cystic forms. It has been assumed that the peculiar clinical and laboratory features of BYS are consequential to the absence of a human sucker Ixodes ricinus complex tick at risk areas in Brazil, supporting the concept that the borrelia phenotypic expression pattern is modified as it is transmitted through the host.

Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes

Onofre,Sideney Becker; Miniuk,Cindia Mara; Barros,Neiva Monteiro de; Azevedo,João Lúcio
Fonte: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz" Publicador: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2001 Português
Relevância na Pesquisa
65.92%
Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae, only few research studies were done in M. flavoviride. The present work was carried out alming to evaluate the Mycelial growth and sporulation of the entomopathogenic fungus Metarhizium flavoviride var. flavoviride growing at 27 ± 2°C on Potato-dextrose-agar (PDA), Czapek-agar (CZP) and a complete agar medium (CM) under three lighting regimes, (continuous illumination, light/dark cycle and an black light/dark cycle) were investigated. A completely randomized 3 × 3 (culture media × lighting regime) factorial design with four replicates was used. The best mycelial growth and sporulation occurred on the PDA and CM media under continuous illumination (P <= 0,05).

Differentiation and numerical analysis of oral yeasts based on SDS-Page profiles. Influence of the culture media on the whole-cell protein extracts

HÖFLING,J. F.; ROSA,E. A. R.; PEREIRA,C. V.; BORIOLLO,M. F. G.; RODRIGUES,J. A. O.
Fonte: Instituto Internacional de Ecologia Publicador: Instituto Internacional de Ecologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2001 Português
Relevância na Pesquisa
66%
The application of gel electrophoresis and numerical analysis of yeast soluble proteins analysis to the investigation of 12 oral yeast strains belonging to five species is described. It involves one-dimensional electrophoresis of SDS-solubilized whole-cell proteins using different culture media for the cultivation of the cells, integration densitometries in the areas of the gels and percentages of the proteins extraction. These extracts were prepared from four isolates of Candida albicans, two of C. tropicalis, C. guilliermondii, C. parapsilosis and C. krusei. The extracts from whole-cells proteins using different culture media for the cultivation of the cells were fractionated by slab electrophoresis using a discontinuous buffer system. The corresponding patterns showed at least 36 polypeptides in the range of 14.4-200 kDa. Different isolates of each species were clearly different in each of the five species. The data obtained suggest that different nutritional compositions led to the expression of different proteins derived from alternatives metabolic pathways expressed by the electrophoretic profiles. The construction of a database of protein fingerprints and numerical analysis based on such data, may have some implications in the classification and identification of such species with epidemiological...

Evaluation of different culture media and enrichment in orange juice upon the growth of Alicyclobacillus spp.

Anjos,Márcia Maria; Ruiz,Suelen Pereira; Abreu Filho,Benício Alves
Fonte: Instituto Biológico Publicador: Instituto Biológico
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2014 Português
Relevância na Pesquisa
65.98%
Bacteria of the genus Alicyiclobacillus spp. form spores and develop in acid media, leading to the spoilage of citrus juices. Brazil is the largest exporter of orange juice concentrate, and yet, it has been extensively studied due to changes in taste and smell. Several investigations have reported different culture media used to detect and enumerate Alicyiclobacillus spp. The objective of the present study was to evaluate the recovery of Alicyiclobacillus spp. spores grown in ALI, BAT, K agar and YSG media using the methodology suggested by ABECitrus. Five inocula were used, two from reference strains and three from pasteurized concentrated orange juice. Cell recovery after the enrichment in reconstituted orange juice was also analyzed. An initial population of 6 log CFU/mL was inoculated. ALI, BAT and YSG media were able to recover the initial population of all different inocula, with no significant differences between the results. When compared to BAT, however, the preparation of ALI and YSG media was simpler and had more advantages. The recovery with K agar medium was lower than the other media for all the tested inocula, with significant differences found for Alicyclobacillus acidocaldarius 0298T (3.66 log CFU/mL) and Alicyclobacillus pomorum-like CBMAI 0278 (4.11 log CFU/mL).

Effect of using multiple culture media for the diagnosis of noninvasive fungal sinusitis

Collins, M.; Nair, S.; Der-Haroutian, V.; Close, D.; Rees, G.; Grove, D.; Wormald, P.J.
Fonte: Ocean Side Publications Inc Publicador: Ocean Side Publications Inc
Tipo: Artigo de Revista Científica
Publicado em //2005 Português
Relevância na Pesquisa
66.05%
Background: This study was designed to assess the relative efficiency of three different culture media for isolating fungi in patients suspected of having noninvasive fungal sinusitis. Methods: A prospective study was performed of 209 operative samples of sinus "fungal-like" mucin from 134 patients on 171 occasions and processed for microscopy and fungal culture in Sabouraud's dextrose agar, potato dextrose agar, and broth media. Results: Ninety-three (69%) of 134 patients had evidence of fungal infection. Two-thirds of patients had negative microscopy samples yet 56% of these went on to positive cultures. Forty-five percent cultured Aspergillus genus. Discrepancy between the fungi cultured in different media and on different occasions was common. With a single culture medium up to 19% of patients and 15% of samples would have been falsely labeled fungal negative. Conclusion: Increasing the number and type of fungal culture media used increases the number and range of fungal isolates from mucin in patients with the features of fungal sinusitis. Negative specimen microscopy is unreliable. All specimens should be cultured in multiple media and on multiple occasions when fungal sinusitis is suspected.; Collins, Melanie M.; Nair, Salil B.; Der-Haroutian...

Influence of culture media on the physical and chemical properties of Ag–TiCN coatings

Carvalho, I.; Escobar Galindo, R.; Henriques, Mariana; Palacio, C.; Carvalho, S.
Fonte: Institute of Physics Publishing Publicador: Institute of Physics Publishing
Tipo: Artigo de Revista Científica
Publicado em //2014 Português
Relevância na Pesquisa
65.92%
The aim of this study was to verify the possible physical and chemical changes that may occur on the surface of Ag–TiCN coatings after exposure to the culture media used in microbiological and cytotoxic assays, respectively tryptic soy broth (TSB) and Dulbecco's modified eagle's medium (DMEM). After sample immersion for 24 h in the media, analyses were performed by glow discharge optical emission spectroscopy discharge radiation (GDOES), Rutherford backscattering spectroscopy (RBS) and x-ray photoelectron spectroscopy (XPS). The results of GDOES profile, RBS and XPS spectra, of samples immersed in TSB, demonstrated the formation of a thin layer of carbon, oxygen and nitrogen that could be due to the presence of proteins in TSB. After 24 h of immersion in DMEM, the results showed the formation of a thin layer of calcium phosphates on the surface, since the coatings displayed a highly oxidized surface in which calcium and phosphorus were detected. All these results suggested that the formation of a layer on the coating surface prevented the release of silver ions in concentrations that allow antibacterial activity.

In vitro embryo production from prepubertal goat oocytes in different culture media

Hammami, Sondes
Fonte: [Barcelona] : Universitat Autònoma de Barcelona, Publicador: [Barcelona] : Universitat Autònoma de Barcelona,
Tipo: Tesis i dissertacions electròniques; info:eu-repo/semantics/doctoralThesis; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em //2014 Português
Relevância na Pesquisa
66.03%
Los ovarios de cabras prepúberes proporcionan una población de ovocitos de pequeño diámetro con una menor competencia para el desarrollo hasta blastocisto después de la maduración, fecundación y posterior cultivo in vitro (MIV, FIV y CIV). Las condiciones del cultivo in vitro aportan los requerimientos necesarios a los ovocitos e influyen sobre su posterior desarrollo embrionario. En esta tesis hemos llevado a cabo dos estudios para evaluar los efectos de la adición de diferentes suplementos a los medios de MIV y CIV sobre el desarrollo embrionario y la calidad de los blastocistos obtenidos a partir de ovocitos de cabras prepúberes. En ovocitos de hembras prepúberes, la adición de sustancias, incluyendo las hormonas gonadotrópicas, factores de crecimiento, fuentes de proteínas y antioxidantes, como son el complejo insulina-transferrina-selenio (ITS) y el L-ácido ascórbico (AA), han demostrado producir una mejora en la maduración citoplasmática de los ovocitos y su subsiguiente desarrollo embrionario in vitro. El objetivo del primer estudio fue evaluar los efectos de la adición de AA, ITS y un bajo nivel hormonal en el medio de MIV sobre el desarrollo embrionario y la calidad de los blastocistos obtenidos. En concreto...

Estudo comparativo de diferentes meios de cultura para o isolamento de salmonelas em matérias-primas e rações; Comparative study of different culture media for salmonella recovery in feedstuffs and feeds

Albuquerque, Ricardo de; Ito, Nair Massako Kotayama; Miyaji, Claudio Issamu
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; Formato: application/pdf
Publicado em 01/01/2000 Português
Relevância na Pesquisa
65.96%
A pesquisa foi conduzida com o objetivo de avaliar a eficiência de meios de isolamento de salmonelas. As matérias-primas e rações analisadas foram submetidas ao pré-enriquecimento em água peptonada tamponada, enriquecimento seletivo nos meios: Selenito cistina (SC), Tetrationato Hajna (TH) e Rappaport Vassiliadis (RV), e semeadura nos meios: Ágar Verde Brilhante (AVB) e Xilose Lisina Desoxicolato (XLD). Verificou-se que os caldos TH e RV produziram mais isolamentos que SC, que foi inferior nas condições deste trabalho, sendo que TH incubado a 42ºC produziu o maior número de resultados positivos, e os meios sólidos de plaqueamento AVB e XLD apresentaram eficiência semelhante.; This work was designed to evaluate the efficacy of culture media for salmonella recovery in feedstuffs and feeds. All experimental samples were processed for recovery of stressed bacteria in buffered peptone water and selective media: Selenite Cystine (SC), Rappaport Vassiliadis (RV) and Tetrathionate Hajna (TH), and solid media: Brilliant Green Agar (BGA) and Xylose Lisine Desoxycholate (XLD). Higher number of positive samples was observed from TH and RV as compared to SC. The TH incubated at 42ºC produces the bigger number of positive results, whereas the solid media BGA and XLD were both effective.

Crescimento e esporulação de Metarhizium flavoviride var. Flavoviride em meios de cultura e regimes de luz; Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes

Onofre, Sideney Becker; Miniuk, Cindia Mara; Barros, Neiva Monteiro de; Azevedo, João Lúcio
Fonte: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz Publicador: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/09/2001 Português
Relevância na Pesquisa
65.92%
Fungos entomopatogênicos do genêro Metarhizium são empregados no controle biológico de pragas agrícolas por meio da dispersão de seus conídios no campo. Embora a produção de conídios em M. anisopliae esteja bem estudada, poucas pesquisas a respeito existem na espécie M. flavoviride. O presente trabalho foi realizado para avaliar o efeito de três meios de cultura, batata-dextrose-ágar (BDA), Czapek-ágar (CZP) e meio completo (MC), e três regimes de luminosidade, claro contínuo, alternância com luz do dia/escuro e luz negra/escuro, sobre o crescimento miceliano e esporulação do fungo entomopatogênico Metarhizium flavoviride var. flavoviride. O experimento foi realizado em câmara de incubação à temperatura de 27 ± 2°C. Empregou-se um delineamento experimental inteiramente casualizado, em arranjo fatorial 3°C 3 (meios de cultura e regime de luminosidade) com quatro repetições. As melhores condições para o crescimento miceliano e esporulação foram conseguidas tanto no meio de cultura BDA como no MC quando combinados com o regime claro contínuo (P; Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae...

Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity

Gende,L. B.; Eguaras,M. J.; Fritz,R.
Fonte: Revista argentina de microbiología Publicador: Revista argentina de microbiología
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2008 Português
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This study was conducted to compare different liquid culture media for Paenibacillus larvae growth in order to find the best one to be used in studies on activity of antimicrobial substances, such as essential oils. P. larvae presented poor growth in usual broths such as Mueller-Hinton, commonly employed in antimicrobial activity assays. Growth in liquid media was evaluated using Paenibacillus larvae strains isolated from hives located in different geographical zones. The MYT medium (Mueller-Hinton broth, yeast extract and thiamine) was selected out of the eight liquid media analyzed, as it proved to be the most adequate due to its higher absorbance at 620 nm. The following mean values were obtained from the four P. larvae strains: 0.227 ± 0.016 for the Cobo strain, 0.279 ± 0.015 for La Plata strain, 0.758 ± 0.020 for Mechongué strain and 0.244 ± 0.0079 for Sierra de los Padres strain, respectively.

Usage of green coconut water and different tissue culture media for in vitro honey bee semen storage (apis mellifera; hymenoptera: apoidea)

Almeida,Rosana; Espencer Egea Soares,Ademilson
Fonte: ASOCIACIÓN INTERCIENCIA Publicador: ASOCIACIÓN INTERCIENCIA
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2002 Português
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65.89%
Honey bee semen was stored in green coconut (Cocos nucifera L.) water plus dihydrostreptomycin and in commercial tissue culture media at different temperatures. Glass capillary microtubes of 0.1cm diameter and centrifuge microtubes 0.2ml capacity were used for semen storage. Sperm motility was assessed after 1, 2, 5, 10, 15, 30, 50, 80 and 120 days. Queens were instrumentally inseminated with diluted semen and their laying behavior evaluated. Storage in coconut water shows living sperm until 80 days. However, the queen’s laying was normal and resulted in viable worker brood only when semen stored up to 15 days in coconut water medium was used for insemination. Coconut water seems to be an ideal natural diluent for short periods in vitro storage of honey bee semen and can be an appropriate method for genetic improvement programmes for honey bees.

Comparison of different culture media and storage temperatures for the long-term preservation of Streptococcus pneumoniaein the tropics

Siberry,George; Brahmadathan,K.N.; Pandian,Rajeswar; Lalitha,M.K.; Steinhoff,Mark C.; John,T. Jacob
Fonte: World Health Organization Publicador: World Health Organization
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2001 Português
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OBJECTIVE: The preservation of Streptococcus pneumoniae by standard freezing methods for subsequent tests - such as serotyping and antibiotic susceptibility - is not possible or is difficult in many developing countries because of the high cost of equipment, inadequate equipment maintenance, and irregular power supply. We evaluated alternative low-cost methods, by comparing different culture media and storage temperatures. METHODS: Clinical isolates of five capsular types (1, 5, 7, 19, and 23) of S. pneumoniae were preserved in rabbit blood, sheep blood, skimmed milk, or glycerol-chocolate broth, and stored at -20 ºC or -70 ºC. The cultures were also preserved by lyophilization or sand desiccation, followed by storage at room temperature and 4 ºC. The viability of the preserved cultures was determined by making serial colony counts on day 0 and after 1 week, 4 weeks, 4 months and 16 months. The viability of cultures preserved by sand desiccation and storage at 4 ºC was also determined every 6 months for up to 68 months. FINDINGS: Irrespective of the media used, cultures maintained at -20 ºC became nonviable by the fourth month, while those maintained at -70 ºC were still viable at 16 months. Cultures preserved by lyophilization or sand desiccation lost their viability by the fourth month when maintained at local room temperature (30-42 ºC)...