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Análise das condições higrotérmicas na Biblioteca Joanina da Universidade de Coimbra

Catarino, Inês Nazaré
Fonte: Universidade de Coimbra Publicador: Universidade de Coimbra
Tipo: Dissertação de Mestrado
Português
Relevância na Pesquisa
15.51%
Esta tese surge no âmbito de um protocolo criado entre a ADAI (Associação para o Desenvolvimento da Aerodinâmica Industrial) e a BGUC (Biblioteca Geral da Universidade de Coimbra), com o objectivo de analisar as condições higrotérmicas (caracterizadas nomeadamente pelos valores da humidade relativa e temperatura) verificadas na Biblioteca Joanina da UC (Universidade de Coimbra). Durante a última década, as condições ambientais das instalações destinadas ao alojamento de material bibliográfico têm-se mostrado um factor crucial relativamente à preservação do mesmo. A poluição, a humidade, a temperatura e a iluminação podem eventualmente deteriorar ou mesmo destruir o espólio bibliográfico que é mantido no interior de bibliotecas. Portanto o objectivo da presente tese é analisar e avaliar as condições higrotérmicas na Biblioteca Joanina, desprovida de sistemas de climatização. Em paralelo, iniciou-se no mês de Julho um estudo das condições higrotérmicas existentes no interior da casa forte da Biblioteca Geral. Através da análise dos valores medidos da temperatura e humidade relativa será possível avaliar o desempenho que o edifício em estudo apresenta para a preservação de acervos bibliográficos. Para a recolha de dados recorreu-se à utilização de sensores higrotérmicos...

Diversidade de cianobactérias na filosfera da mata atlântica do estado de São Paulo; Cyanobacterial diversity in the phyllosphere of the Atlantic Forest of São Paulo state

Gonçalves, Natalia Juliana Nardelli
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 01/07/2011 Português
Relevância na Pesquisa
25.7%
A Mata Atlântica brasileira, atualmente, está reduzida a pequenos fragmentos florestais protegidos em unidades de conservação, que representam apenas 7,6% de sua área original. O Parque Estadual da Serra do Mar (PESM), localizado no estado de São Paulo é a maior área de proteção integral deste bioma do litoral brasileiro. A superfície da folha de árvore (filosfera) oferece uma grande área de habitat para os micro-organismos, mas constitui um ambiente extremo. O desenvolvimento de comunidades microbianas é dependente de fonte de carbono e de nutrientes essenciais inorgânicos comumente liberados pela planta para a sua superfície. No entanto, um grupo especial de bactérias, Cyanobacteria, é menos dependente da planta para sua nutrição, pois estes organismos são autotróficos para carbono e nitrogênio. Portanto, Cyanobacteria é particularmente interessante para se avaliar neste ambiente. Neste estudo, a comunidade de cianobactérias colonizadoras das filosferas de uma área de conservação (PESM) da floresta ombrófila densa foi avaliada usando abordagens moleculares independente de cultivo. O DNA genômico de cianobactérias foi extraído da superfície de folhas das árvores Euterpe edulis e Guapira opposita de dois locais dentro do Parque...

Memória do Rio Grande do Sul : critérios de seleção de obras raras e valiosas para digitalização

Antunes, Elizana da Rosa
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Trabalho de Conclusão de Curso Formato: application/pdf
Português
Relevância na Pesquisa
25.55%
Dentro do planejamento de uma Biblioteca Digital a seleção de documentos caracteriza-se como o primeiro passo. Este trabalho acadêmico focaliza a primeira etapa desse planejamento e propõe a seleção de obras raras e valiosas do Setor de Documentação do Rio Grande do Sul da Biblioteca Pública do Estado para a digitalização e disponibilização na web, ocasião da criação de uma biblioteca digital. São abordados temas referentes a digitalização, direito autoral, critérios de raridade, conservação, seleção de documentos, informações sobre a Biblioteca Pública e o Setor de Documentação do Rio Grande do Sul. A metodologia adotada é de caráter descritivo e quali-quantiativo. São analisados os dados estatísticos do Setor, com a pesquisa de uma das classes de assuntos que compõe o acervo. Também são considerados na investigação os critérios de raridade, direito autoral e estado de conservação. O produto final desse trabalho é uma lista de obras raras e valiosas que podem vir a integrar uma biblioteca digital. Tudo isso, em função de que foi constatado que há uma necessidade da biblioteca divulgar e colocar junto à comunidade usuária, principalmente pesquisadores, seu acervo raro e valioso, com obras que abordam os mais diferentes temas relacionados ao Estado. Além disso...

Diversidade genética molecular e reação a doenças de acessos silvestres e comerciais de maracujazeiro (Passiflora spp.) presentes em bancos de germoplasma = : Molecular genetic diversity and disease reaction of commercial and wild access of passion fruit (Passiflora spp.) present in germplasm banks; Molecular genetic diversity and disease reaction of commercial and wild access of passion fruit (Passiflora spp.) present in germplasm banks

Carlos Bernard Moreno Cerqueira Silva
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 07/07/2014 Português
Relevância na Pesquisa
15.55%
O gênero Passiflora, cujas espécies são popularmente conhecidas como maracujazeiros, destaca-se na família Passifloraceae tanto pelo número de espécies (aproximadamente 520) quanto pela importância econômica associada à parte destas espécies. Os maracujazeiros ocorrem em diferentes países, sendo sua diversidade amplamente representada nas Américas, onde a Colômbia e o Brasil se destacam com aproximadamente 170 e 150 espécies de Passiflora, respectivamente. Economicamente os maracujazeiros despertam interesse pela beleza de suas flores, presença de princípios ativos medicinais, extração de óleos essenciais para indústria de cosméticos, produção de frutos para consumo in natura ou produção de derivados. O Brasil se destaca como maior produtor de maracujá, embora a produtividade nacional seja baixa (média de 14 T/ha-1 ano-1), quando comparada ao potencial da passicultura (± 50 T/ha -1 ano-1). Em parte essa baixa produtividade é ocasionada pela ausência de cultivares adaptadas às diferentes regiões produtoras e pela suscetibilidade das cultivares às principais enfermidades que acometem a cultura. Embora crescente, os programas de melhoramento genético do maracujazeiro apresentam resultados modestos frente às demandas existentes. Entre os obstáculos enfrentados pelos melhoristas...

MicroRNAome of Porcine Pre- and Postnatal Development

Li, Mingzhou; Xia, Youlin; Gu, Yiren; Zhang, Kai; Lang, Qiulei; Chen, Lei; Guan, Jiuqiang; Luo, Zonggang; Chen, Haosi; Li, Yang; Li, Qinghai; Li, Xiang; Jiang, An-an; Shuai, Surong; Wang, Jinyong; Zhu, Qi; Zhou, Xiaochuan; Gao, Xiaolian; Li, Xuewei
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 12/07/2010 Português
Relevância na Pesquisa
15.51%
The domestic pig is of enormous agricultural significance and valuable models for many human diseases. Information concerning the pig microRNAome (miRNAome) has been long overdue and elucidation of this information will permit an atlas of microRNA (miRNA) regulation functions and networks to be constructed. Here we performed a comprehensive search for porcine miRNAs on ten small RNA sequencing libraries prepared from a mixture of tissues obtained during the entire pig lifetime, from the fetal period through adulthood. The sequencing results were analyzed using mammalian miRNAs, the precursor hairpins (pre-miRNAs) and the first release of the high-coverage porcine genome assembly (Sscrofa9, April 2009) and the available expressed sequence tag (EST) sequences. Our results extend the repertoire of pig miRNAome to 867 pre-miRNAs (623 with genomic coordinates) encoding for 1,004 miRNAs, of which 777 are unique. We preformed real-time quantitative PCR (q-PCR) experiments for selected 30 miRNAs in 47 tissue-specific samples and found agreement between the sequencing and q-PCR data. This broad survey provides detailed information about multiple variants of mature sequences, precursors, chromosomal organization, development-specific expression...

Consensus Protein Design Without Phylogenetic Bias

Jäckel, Christian; Bloom, Jesse D; Kast, Peter; Arnold, Frances H; Hilvert, Donald
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
15.55%
Consensus design is an appealing strategy for the stabilization of proteins. It exploits amino acid conservation in sets of homologous proteins to identify likely beneficial mutations. Nevertheless, its success depends on the phylogenetic diversity of the sequence set available. Here we show that randomization of a single protein represents a reliable alternative source of sequence diversity essentially free of phylogenetic bias. A small number of functional protein sequences selected from binary-patterned libraries suffices as input for consensus design of active enzymes that are easier to produce and substantially more stable than individual members of the starting data set. Although catalytic activity correlates less consistently with sequence conservation in these extensively randomized proteins, less extreme mutagenesis strategies might be adopted in practice to augment stability while maintaining function.

Transcriptomic landscape of prophase I sunflower male meiocytes

Flórez-Zapata, Nathalia M. V.; Reyes-Valdés, M. H.; Hernandez-Godínez, Fernando; Martínez, Octavio
Fonte: Frontiers Media S.A. Publicador: Frontiers Media S.A.
Tipo: Artigo de Revista Científica
Publicado em 16/06/2014 Português
Relevância na Pesquisa
15.56%
Meiosis is a form of specialized cell division that generates gametes, allowing recombination of alleles and halving the chromosome number. Arabidopsis and maize are the plant models that have been most extensively studied to determine the genes involved in meiosis. Here we present an RNA-seq study in which gene expression in male meiocytes isolated during prophase I was compared to that in somatic tissues of the sunflower HA89 line. We sampled more than 490 million gene tags from these libraries, assembled them de novo into a sunflower transcriptome. We obtained expression data for 36,304 sunflower genes, of which 19,574 (54%) were differentially expressed (DE) between meiocytes and somatic tissue. We also determined the functional categories and metabolic pathways that are DE in these libraries. As expected, we found large differences between the meiotic and somatic transcriptomes, which is in accordance with previous studies in Arabidopsis and maize. Furthermore, most of the previously implicated meiotic genes were abundantly and DE in meiocytes and a large repertoire of transcription factors (TF) and genes related to silencing are expressed in the sunflower meiocytes. We detected TFs which appear to be exclusively expressed in meiocytes. Our results allow for a better understanding of the conservation and differences in the meiotic transcriptome of plants.

Cyberdiversity: Improving the Informatic Value of Diverse Tropical Arthropod Inventories

Miller, Jeremy A.; Miller, Joshua H.; Pham, Dinh-Sac; Beentjes, Kevin K.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 26/12/2014 Português
Relevância na Pesquisa
15.55%
In an era of biodiversity crisis, arthropods have great potential to inform conservation assessment and test hypotheses about community assembly. This is because their relatively narrow geographic distributions and high diversity offer high-resolution data on landscape-scale patterns of biodiversity. However, a major impediment to the more widespread application of arthropod data to a range of scientific and policy questions is the poor state of modern arthropod taxonomy, especially in the tropics. Inventories of spiders and other megadiverse arthropods from tropical forests are dominated by undescribed species. Such studies typically organize their data using morphospecies codes, which make it difficult for data from independent inventories to be compared and combined. To combat this shortcoming, we offer cyberdiversity, an online community-based approach for reconciling results of independent inventory studies where current taxonomic knowledge is incomplete. Participating scientists can upload images and DNA barcode sequences to dedicated databases and submit occurrence data and links to a web site (www.digitalSpiders.org). Taxonomic determinations can be shared with a crowdsourcing comments feature, and researchers can discover specimens of interest available for loan and request aliquots of genomic DNA extract. To demonstrate the value of the cyberdiversity framework...

Next Generation Semiconductor Based Sequencing of the Donkey (Equus asinus) Genome Provided Comparative Sequence Data against the Horse Genome and a Few Millions of Single Nucleotide Polymorphisms

Bertolini, Francesca; Scimone, Concetta; Geraci, Claudia; Schiavo, Giuseppina; Utzeri, Valerio Joe; Chiofalo, Vincenzo; Fontanesi, Luca
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 07/07/2015 Português
Relevância na Pesquisa
25.51%
Few studies investigated the donkey (Equus asinus) at the whole genome level so far. Here, we sequenced the genome of two male donkeys using a next generation semiconductor based sequencing platform (the Ion Proton sequencer) and compared obtained sequence information with the available donkey draft genome (and its Illumina reads from which it was originated) and with the EquCab2.0 assembly of the horse genome. Moreover, the Ion Torrent Personal Genome Analyzer was used to sequence reduced representation libraries (RRL) obtained from a DNA pool including donkeys of different breeds (Grigio Siciliano, Ragusano and Martina Franca). The number of next generation sequencing reads aligned with the EquCab2.0 horse genome was larger than those aligned with the draft donkey genome. This was due to the larger N50 for contigs and scaffolds of the horse genome. Nucleotide divergence between E. caballus and E. asinus was estimated to be ~ 0.52-0.57%. Regions with low nucleotide divergence were identified in several autosomal chromosomes and in the whole chromosome X. These regions might be evolutionally important in equids. Comparing Y-chromosome regions we identified variants that could be useful to track donkey paternal lineages. Moreover, about 4.8 million of single nucleotide polymorphisms (SNPs) in the donkey genome were identified and annotated combining sequencing data from Ion Proton (whole genome sequencing) and Ion Torrent (RRL) runs with Illumina reads. A higher density of SNPs was present in regions homologous to horse chromosome 12...

Highly conserved gene order and numerous novel repetitive elements in genomic regions linked to wing pattern variation in Heliconius butterflies

Papa, Riccardo; Morrison, Clayton M; Walters, James R; Counterman, Brian A; Chen, Rui; Halder, Georg; Ferguson, Laura; ffrench-Constant, Richard; Kapan, Durrell D; Jiggins, Chris D; Chamberlain, Nicola L; Reed, Robert D.; McMillan, William O.
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
15.55%
Background: With over 20 parapatric races differing in their warningly colored wing patterns, the butterfly Heliconius erato provides a fascinating example of an adaptive radiation. Together with matching races of its co-mimic Heliconius melpomene, H. erato also represents a textbook case of Müllerian mimicry, a phenomenon where common warning signals are shared amongst noxious organisms. It is of great interest to identify the specific genes that control the mimetic wing patterns of H. erato and H. melpomene. To this end we have undertaken comparative mapping and targeted genomic sequencing in both species. This paper reports on a comparative analysis of genomic sequences linked to color pattern mimicry genes in Heliconius. Results: Scoring AFLP polymorphisms in H. erato broods allowed us to survey loci at approximately 362 kb intervals across the genome. With this strategy we were able to identify markers tightly linked to two color pattern genes: D and Cr, which were then used to screen H. erato BAC libraries in order to identify clones for sequencing. Gene density across 600 kb of BAC sequences appeared relatively low, although the number of predicted open reading frames was typical for an insect. We focused analyses on the D- and Cr-linked H. erato BAC sequences and on the Yb-linked H. melpomene BAC sequence. A comparative analysis between homologous regions of H. erato (Cr-linked BAC) and H. melpomene (Yb-linked BAC) revealed high levels of sequence conservation and microsynteny between the two species. We found that repeated elements constitute 26% and 20% of BAC sequences from H. erato and H. melpomene respectively. The majority of these repetitive sequences appear to be novel...

Análise da degradação de amostras de papelexpostas a diferentes condições higrotérmicas

Fritoli, Clara Landim
Fonte: Curitiba Publicador: Curitiba
Tipo: Dissertação de Mestrado
Português
Relevância na Pesquisa
25.7%
In this study, paper samples were produced and naturally exposed to varying temperature and humidity conditions at two university libraries, located in Curitiba (910m a.m.s.l.) and in Caiobá, on the coast of Paraná state. The paper samples were produced with unloaded long-and short-fiber cellulose, without adhesives, in order to facilitate the analysis of the relationship between sample decay rate and natural ageing conditions at both libraries, relative to outdoors. The purpose of the research was to identify which climatic context presents the highest paper preservation potential, by means of the preventive conservation indices PI and TWPI (Preservation Index and the Time Weighted Preservation Index), which quantify the effects of temperature and humidity on paper life expectancy. The samples were placed in naturally ventilated acrylic containers covered with UV film, whose openings were protected with activated charcoal so that temperature and humidity had the major effects on the paper samples. For outdoors, Stevenson screens were used, wherein the acrylic containers were placed. Climatic data were recorded with data loggers and the paper samples were tested with regard to physical and chemical properties before and after a 12and 24 months natural exposure. Results indicate the largest decay rates for the samples with the shortest life expectancy according to PI/TWPI (obtained from ambient temperature and relative humidity) estimates.; CAPES; CNPq; No presente estudo...

Using Sediment Core Data to Better Understand the Ontogeny and Eutrophication of Shallow Florida Lakes

Kenney, William Francis, III
Fonte: University of Florida ( [Gainesville, Fla.] ) Publicador: University of Florida ( [Gainesville, Fla.] )
Tipo: Artigo de Revista Científica Formato: 1 online resource (103 p.)
Publicado em //2015 Português
Relevância na Pesquisa
35.48%
In the series University of Florida Digital Collections.; Includes vita.; (Bibliography) Includes bibliographical references.; (Source of Description) Description based on online resource; title from PDF title page.; (Source of Description) This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.; (Thesis) Thesis (Ph.D.)--University of Florida, 2015.; (Local) Adviser: PHLIPS,EDWARD J.; (Local) Co-adviser: BRENNER,MARK.; (Statement of Responsibility) by William Francis Kenney III.

Fragment-based methods for structure determination with sparse data; Fragmentbasierte Methoden zur Strukturbestimmung mit spärlichen Daten

Kalev, Ivan
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
25.73%
The fragment assembly approach was initially conceived as an ab initio strategy for protein structure prediction; however, the use of fragment libraries is becoming an increasingly popular choice in protein structure determination as well. While most method development efforts have been rightfully directed towards optimization of the fragment assembly algorithms, we show that their performance is also strongly dependent on the quality of the underlying fragment libraries. Here we present an integrated, general-purpose framework for construction of dynamic fragment libraries and local structure prediction from sequence. Our method, HHfrag, uses sensitive local alignment of sequence profiles to detect recurrent protein motifs of local conservation and build fragment libraries of very high precision and truly dynamic fragment lengths, solving important limitations of earlier methods. We demonstrate that this approach improves the performance of traditional ab initio fragment assembly and introduce algorithms for fragment clustering, filtering and blind prediction of local motif conservation. The resulting confidence-guided framework for local structure prediction is a solid foundation for context-sensitive torsion angle prediction with higher accuracy. Finally...

Análise das Condições Higrotérmicas em Espaços da Biblioteca Geral da Universidade de Coimbra

Rocha, Nélida Cristina Delgado
Fonte: Universidade de Coimbra Publicador: Universidade de Coimbra
Tipo: Dissertação de Mestrado
Português
Relevância na Pesquisa
25.62%
O controlo das condições higrotérmicas em bibliotecas é indispensável para a preservação e conservação dos seus acervos. É neste contexto que se enquadra a presente dissertação, realizada no decorrer de um protocolo criado entre a ADAI (Associação para o Desenvolvimento da Aerodinâmica Industrial) e a BGUC (Biblioteca Geral da Universidade de Coimbra). O principal objectivo é analisar e avaliar as condições higrotérmicas (valores da humidade relativa e temperatura) verificadas em três espaços da BGUC, nomeadamente, o depósito de periódicos, o depósito de livros e o depósito de manuscritos musicais. Inicialmente fez-se a inspecção visual dos espaços, onde foi possível recolher toda a informação necessária para a descrição destes. Para a avaliação do desempenho que o edifício apresenta para a preservação dos seus acervos, recorreu-se a recolha de dados de humidade relativa e temperatura ao longo de um determinado período de tempo utilizando sensores higrotérmicos. Posteriormente procedeu-se ao tratamento dos mesmos, através da utilização de fórmulas matemáticas e programas informáticos (KaleidaGraph e Excel). Determinou-se também a taxa de renovação de ar no depósito de manuscritos musicais...

Natural sound archives: past, present and future

Ranft,Richard
Fonte: Academia Brasileira de Ciências Publicador: Academia Brasileira de Ciências
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2004 Português
Relevância na Pesquisa
25.56%
Recordings of wild animals were first made in the Palearctic in 1900, in the Nearctic in 1929, in Antarctica in 1934, in Asia in 1937, and in the Neotropics in the 1940s. However, systematic collecting did not begin until the 1950s. Collections of animal sound recordings serve many uses in education, entertainment, science and nature conservation. In recent years, technological developments have transformed the ways in which sounds can be sampled, stored and accessed. Now the largest collections between them hold altogether around 0.5 million recordings with their associated data. The functioning of a major archive will be described with reference to the British Library Sound Archive. Preserving large collections for the long term is a primary concern in the digital age. While digitization and digital preservation has many advantages over analogue methods, the rate of technology change and lack of standardization are a serious problem for theworld's major audio archives. Another challenge is to make collections more easily and widely accessible via electronic networks. On-line catalogues and access to the actual sounds via the internet are already available for some collections. Case studies describing the establishment and functioning of sound libraries inMexico...

Physicochemical Characterization of a Thermostable Alcohol Dehydrogenase from Pyrobaculum aerophilum

Vitale, Annalisa; Thorne, Natasha; Lovell, Scott; Battaile, Kevin P.; Hu, Xin; Shen, Min; D'Auria, Sabato; Auld, Douglas S.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 05/06/2013 Português
Relevância na Pesquisa
15.6%
In this work we characterize an alcohol dehydrogenase (ADH) from the hyperthermophilic archaeon Pyrobaculum aerophilum (PyAeADHII). We have previously found that PyAeADHII has no activity when standard ADH substrates are used but is active when α-tetralone is used as substrate. Here, to gain insights into enzyme function, we screened several chemical libraries for enzymatic modulators using an assay employing α-tetralone. The results indicate that PyAeADHII activity in the presence of α-tetralone was inhibited by compounds such as flunarizine. We also examined metal coordination of the enzyme in solution by performing metal substitution of the enzyme-bound zinc (Zn2+) with cobalt. The solution-based absorption spectra for cobalt substituted PyAeADHII supports substitution at the structural Zn2+ site. To gain structural insight, we obtained the crystal structure of both wild-type and cobalt-substituted PyAeADHII at 1.75 Å and 2.20 Å resolution, respectively. The X-ray data confirmed one metal ion per monomer present only at the structural site with otherwise close conservation to other ADH enzymes. We next determined the co-crystal structure of the NADPH-bound form of the enzyme at 2.35 Å resolution to help define the active site region of the enzyme and this data shows close structural conservation with horse ADH...

A multilingual/multicultural semantic-based approach to improve Data Sharing in an SDI for Nature Conservation

De Martino, Monica; Albertoni, Riccardo
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Publicado em 08/07/2011 Português
Relevância na Pesquisa
35.67%
The paper proposes an approach to transcend multicultural and multilingual barriers in the use and reuse of geographical data at the European level. The approach aims at sharing scientific terms in the field of nature conservation with the goal of assisting different user communities with metadata compilation and information discovery. A multi-thesauri solution is proposed, based on a Common Thesaurus Framework for Nature Conservation, where different well-known Knowledge Organization Systems are assembled and shared. It has been designed according to semantic web and W3C recommendations employing SKOS standard models and Linked Data to publish the thesauri as a whole in machine-understandable format. The outcome is a powerful framework satisfying the requirements of modularity and openness for further thesaurus extension and updating, interlinking among thesauri, and exploitability from other systems. The paper supports the employment of Linked Data to deal with terminologies in complex domains such as nature conservation and it proposes a hands-on recipe to publish thesauri in the framework.

Consensus Protein Design without Phylogenetic Bias

Jäckel, Christian; Bloom, Jesse D.; Kast, Peter; Arnold, Frances H.; Hilvert, Donald
Fonte: Elsevier Publicador: Elsevier
Tipo: Article; PeerReviewed Formato: application/pdf; application/msword
Publicado em 18/06/2010 Português
Relevância na Pesquisa
15.55%
Consensus design is an appealing strategy for the stabilization of proteins. It exploits amino acid conservation in sets of homologous proteins to identify likely beneficial mutations. Nevertheless, its success depends on the phylogenetic diversity of the sequence set available. Here, we show that randomization of a single protein represents a reliable alternative source of sequence diversity that is essentially free of phylogenetic bias. A small number of functional protein sequences selected from binary-patterned libraries suffice as input for the consensus design of active enzymes that are easier to produce and substantially more stable than individual members of the starting data set. Although catalytic activity correlates less consistently with sequence conservation in these extensively randomized proteins, less extreme mutagenesis strategies might be adopted in practice to augment stability while maintaining function.

Comparative genomic data of the Avian Phylogenomics Project.

Zhang, G; Li, B; Li, C; Gilbert, MT; Jarvis, ED; Wang, J; Avian Genome Consortium
Fonte: Universidade Duke Publicador: Universidade Duke
Publicado em //2014 Português
Relevância na Pesquisa
25.61%
BACKGROUND: The evolutionary relationships of modern birds are among the most challenging to understand in systematic biology and have been debated for centuries. To address this challenge, we assembled or collected the genomes of 48 avian species spanning most orders of birds, including all Neognathae and two of the five Palaeognathae orders, and used the genomes to construct a genome-scale avian phylogenetic tree and perform comparative genomics analyses (Jarvis et al. in press; Zhang et al. in press). Here we release assemblies and datasets associated with the comparative genome analyses, which include 38 newly sequenced avian genomes plus previously released or simultaneously released genomes of Chicken, Zebra finch, Turkey, Pigeon, Peregrine falcon, Duck, Budgerigar, Adelie penguin, Emperor penguin and the Medium Ground Finch. We hope that this resource will serve future efforts in phylogenomics and comparative genomics. FINDINGS: The 38 bird genomes were sequenced using the Illumina HiSeq 2000 platform and assembled using a whole genome shotgun strategy. The 48 genomes were categorized into two groups according to the N50 scaffold size of the assemblies: a high depth group comprising 23 species sequenced at high coverage (>50X) with multiple insert size libraries resulting in N50 scaffold sizes greater than 1 Mb (except the White-throated Tinamou and Bald Eagle); and a low depth group comprising 25 species sequenced at a low coverage (~30X) with two insert size libraries resulting in an average N50 scaffold size of about 50 kb. Repetitive elements comprised 4%-22% of the bird genomes. The assembled scaffolds allowed the homology-based annotation of 13...

The science of digitizing paintings for color-accurate image archives: a review

Berns, Roy
Fonte: The Society for Imaging Science and Technology (IS&T) Publicador: The Society for Imaging Science and Technology (IS&T)
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
15.56%
A review of the human visual system, the CIE L*,a*,b*color space and its use in evaluating color image quality, and digital image capture is presented,the goal of which is to provide background information for imaging professionals involved in creating digital image databases for museums, galleries, archives, and libraries. Following this review,an analysis was performed to determine the effects of bit depth, dynamic range,gamma correction,and color correction on the ability to estimate colorimetric data from R,G,B digital images with a minimum of error.The proper use of gray scale and color targets was also considered. Recommendations are presented for the direct digital image capture of paintings. Finally, a brief look into the future using spectral imaging techniques is presented.; RIT community members may access full-text via RIT Libraries licensed databases: http://library.rit.edu/databases/; This publication was written while the author was a Senior Fellow in Conservation Science at the National Gallery of Art, Washington; the Gallery’s financial support is sincerely appreciated. The author gratefully acknowledges the assistance of Janet Bliburg, Lorene Emerson, and Lyle Peterzell in collecting digital and conventional images...