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Fibroblast Growth Factor 23 in Hemodialysis Patients: Effects of Phosphate Binder, Calcitriol and Calcium Concentration in the Dialysate

CANCELA, Ana L. E.; OLIVEIRA, Rodrigo B.; GRACIOLLI, Fabiana G.; REIS, Luciene M. dos; BARRETO, Fellype; BARRETO, Daniela V.; CUPPARI, Lilian; JORGETTI, Vanda; CARVALHO, Aluizio B.; CANZIANI, Maria Eugenia; MOYSES, Rosa M. A.
Fonte: KARGER Publicador: KARGER
Tipo: Artigo de Revista Científica
Português
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Background: Fibroblast growth factor 23 (FGF23) concentrations increase early in chronic kidney disease (CKD), and the influence of current CKD-mineral and bone disorder (MBD) therapies on serum FGF23 levels is still under investigation. Methods: In this post-hoc analysis of a randomized clinical trial, phosphate binders and calcitriol were washed out of 72 hemodialysis patients who were then submitted to bone biopsy, coronary tomography and biochemical measures, including FGF23. They were randomized to receive sevelamer or calcium acetate for 1 year and the prescription of calcitriol and the calcium concentration in the dialysate were adjusted according to serum calcium, phosphate and PTH and bone biopsy diagnosis. Results: At baseline, bone biopsy showed that 58.3% had low-turnover bone disease, whereas 38.9% had high-turnover bone disease, with no significant differences between them with regard to FGF23. Median baseline FGF23 serum levels were elevated and correlated positively with serum phosphate. After 1 year, serum FGF23 decreased significantly. Repeated measures ANOVA analysis showed that the use of a 3.5-mEq/l calcium concentration in the dialysate, as well as the administration of calcitriol and a calcium-based phosphate binder were associated with higher final serum FGF23 levels. Conclusions: Taken together...

Involvement of Astroglial Fibroblast Growth Factor-2 and Microglia in the Nigral 6-OHDA Parkinsonism and a Possible Role of Glucocorticoid Hormone on the Glial Mediated Local Trophism and Wound Repair

SILVA, Camila; FUXE, Kjell; CHADI, Gerson
Fonte: SPRINGER Publicador: SPRINGER
Tipo: Artigo de Revista Científica
Português
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We have observed in previous studies that 6-hydroxydopamine (6-OHDA)-induced lesions in the nigrostriatal dopamine (DA) system promote increases of the astroglial basic fibroblast growth factor (FGF-2, bFGF) synthesis in the ascending DA pathways, event that could be modified by adrenosteroid hormones. Here, we first evaluated the changes of microglial reactivity in relation to the FGF-2-mediated trophic responses in the lesioned nigrostriatal DA system. 6-OHDA was injected into the left side of the rat substantia nigra. The OX42 immunohistochemistry combined with stereology showed the time course of the microglial activation. The OX42 immunoreactivity (IR) was already increased in the pars compacta of the substantia nigra (SNc) and ventral tegmental area (VTA) 2 h after the 6-OHDA injection, peaked on day 7, and remained increased on the 14th day time-interval. In the neostriatum, OX42 immunoreactive (ir) microglial profiles increased at 24 h, peaked at 72 h, was still increased at 7 days but not 14 days after the 6-OHDA injection. Two-colour immunofluorescence analysis of the tyrosine hydroxylase (TH) and OX42 IRs revealed the presence of small patches of TH IR within the activated microglia. A decreased FGF-2 IR was seen in the cytoplasm of DA neurons of the SNc and VTA as soon as 2 h after 6-OHDA injection. The majority of the DA FGF-2 ir cells of these regions had disappeared 72 h after neurotoxin. The astroglial FGF-2 IR increased in the SNc and VTA...

Expression of fibroblast growth factor 10 and its receptor, fibroblast growth factor receptor 2B, in the bovine corpus luteum

CASTILHO, A. C.; GIOMETTI, I. C.; BERISHA, B.; SCHAMS, D.; PRICE, C. A.; AMORIM, R. L.; PAPA, P. C.; BURATINI JR., J.
Fonte: WILEY-LISS Publicador: WILEY-LISS
Tipo: Artigo de Revista Científica
Português
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There is evidence that several fibroblast growth factors (FGFs) are involved in growth and development of the corpus luteum (CL), but many FGFs have not been investigated in this tissue, including FGF10. The objective of this study was to determine if FGF10 and its receptor (FGFR2B) are expressed in the CL. Bovine CL were collected from an abattoir and classed as corpus hemorrhagica (stage 1), developing (stage 11), developed (stage 111), and regressed (stage IV) CL. Expression of FGF10 and FGFR2B mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR). Both genes were expressed in bovine CL, and FGF10 expression did not differ between stages of CL development. FGF10 protein was localized to large and small luteal cells by immunohistochemistry. FGFR2B expression was approximately threefold higher in regressed compared to developing and developed CL (P < 0.05). To determine if FGF10 and FGFR2B expression is regulated during functional luteolysis, cattle were injected with PGF2 alpha and CL collected at 0, 0.5, 2, 4, 12, 24, 48, and 64 hr thereafter (n = 5 CL/time point), and mRNA abundance was measured by real-time RT-PCR. FGF10 mRNA expression did not change during functional luteolysis, whereas FGFR2B mRNA abundance decreased significantly at 2...

Expression of fibroblast growth factor receptors during development and regression of the bovine corpus luteum

GUERRA, D. M.; GIOMETTI, I. C.; PRICE, C. A.; ANDRADE, P. B.; CASTILHO, A. C.; MACHADO, M. F.; RIPAMONTE, P.; PAPA, P. C.; BURATINI JR., J.
Fonte: CSIRO PUBLISHING Publicador: CSIRO PUBLISHING
Tipo: Artigo de Revista Científica
Português
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There is evidence that fibroblast growth factors (FGFs) are involved in the regulation of growth and regression of the corpus luteum (CL). However, the expression pattern of most FGF receptors (FGFRs) during CL lifespan is still unknown. The objective of the present study was to determine the pattern of expression of `B` and `C` splice variants of FGFRs in the bovine CL. Bovine CL were collected from an abattoir and classed as corpora hemorrhagica (Stage I), developing (Stage II), developed (Stage III) or regressed (Stage IV) CL. Expression of FGFR mRNA was measured by semiquantitative reverse transcription-polymerase chain reaction and FGFR protein was localised by immunohistochemistry. Expression of mRNA encoding the `B` and `C` spliced forms of FGFR1 and FGFR2 was readily detectable in the bovine CL and was accompanied by protein localisation. FGFR1C and FGFR2C mRNA expression did not vary throughout CL lifespan, whereas FGFR1B was upregulated in the developed (Stage III) CL. FGFR3B, FGFR3C and FGFR4 expression was inconsistent in the bovine CL. The present data indicate that FGFR1 and FGFR2 splice variants are the main receptors for FGF action in the bovine CL.

Efeito da terapia com laser em baixa intensidade (LILT) na expressão de fatores de crescimento da família FGF por fibroblastos gengivais humanos; Low-intensity laser therapy (LILT) effects on fibroblast growth factors expression by human gingival fibroblasts

Damante, Carla Andreotti
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 18/06/2007 Português
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A terapia com laser em baixa intensidade, conhecida como Low-intensity laser therapy (LILT), tem sido alvo de pesquisas que buscam esclarecer os mecanismos pelos quais o laser age na cicatrização de feridas. Este processo é dependente da transmissão de sinais entre epitélio e conjuntivo, os quais influenciam a proliferação e a migração celular, onde participam fatores de crescimento. O objetivo desta pesquisa foi verificar os efeitos da radiação com lasers vermelho e infravermelho em baixa intensidade na produção de fatores de crescimento por fibroblastos in vitro. Foi obtida uma cultura primária de fibroblastos gengivais humanos (linhagem FGH). As células foram cultivadas em placas de 96 poços (5 x 103 células /poço), em estado de quiescência (meio de cultura suplementado com 1% de soro fetal bovino) e irradiadas com lasers de diodo (AlGaAs - 660nm, e AlGaInP - 780nm). O laser em modo contínuo, foi aplicado em contato e na forma pontual. A potência utilizada foi de 40mW numa área de 0,042cm2 e com densidades de energia de 2, 3, 4, 5 e 6J/cm² com respectivos tempos de aplicação de 2, 3, 4, 5 e 6s. As culturas foram irradiadas duas vezes com 6h de intervalo. Os grupos controle positivo e negativo foram cultivados com 10% e 1% de soro fetal bovino...

Expression of fibroblast growth factor 10 and its receptor, fibroblast growth factor receptor 2B, in the bovine corpus luteum

Castilho, A. C.; Giometti, I. C.; Berisha, B.; Schams, D.; Price, C. A.; Amorim, R. L.; Papa, P. C.; Buratini, J.
Fonte: Wiley-liss Publicador: Wiley-liss
Tipo: Artigo de Revista Científica Formato: 940-945
Português
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36.54%
There is evidence that several fibroblast growth factors (FGFs) are involved in growth and development of the corpus luteum (CL), but many FGFs have not been investigated in this tissue, including FGF10. The objective of this study was to determine if FGF10 and its receptor (FGFR2B) are expressed in the CL. Bovine CL were collected from an abattoir and classed as corpus hemorrhagica (stage 1), developing (stage 11), developed (stage 111), and regressed (stage IV) CL. Expression of FGF10 and FGFR2B mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR). Both genes were expressed in bovine CL, and FGF10 expression did not differ between stages of CL development. FGF10 protein was localized to large and small luteal cells by immunohistochemistry. FGFR2B expression was approximately threefold higher in regressed compared to developing and developed CL (P < 0.05). To determine if FGF10 and FGFR2B expression is regulated during functional luteolysis, cattle were injected with PGF2 alpha and CL collected at 0, 0.5, 2, 4, 12, 24, 48, and 64 hr thereafter (n = 5 CL/time point), and mRNA abundance was measured by real-time RT-PCR. FGF10 mRNA expression did not change during functional luteolysis, whereas FGFR2B mRNA abundance decreased significantly at 2...

Expression of fibroblast growth factor-8 and its cognate receptors, fibroblast growth factor receptor (FGFR)-3c and-4, in fetal bovine preantral follicles

Buratini, J.; Glapinski, V. F.; Giometti, I. C.; Teixeira, A. B.; Costa, I. B.; Avellar, MCW; Barros, C. M.; Price, C. A.
Fonte: Wiley-Blackwell Publicador: Wiley-Blackwell
Tipo: Artigo de Revista Científica Formato: 255-261
Português
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Paracrine cell signaling is thought to be important for ovarian follicle development, and a role for some members of the fibroblast growth factor (FGF) family have been suggested. In the present study, we tested the hypothesis that FGF-8 and its cognate receptors (FGFR-3c and FGFR-4) are expressed in bovine preantral follicles. Reverse transcription-polymerase chain reaction was used to amplify bovine FGF-8, FGFR-3c, and FGFR-4 from preantral follicle samples and a variety of fetal and adult tissues. All three genes were widely expressed in fetal tissues, with a restricted expression pattern in adult tissues. FGF-8 and FGFR-3c were expressed in secondary follicles in 70% of fetuses examined, whereas FGFR-4 expression was significantly less frequent (20%). FGFR-3c expression frequency was significantly lower in primordial compared to secondary follicles, and FGF-8 expression showed a similar trend. FGFR-4 was only observed when all follicle classes of an individual were expressing both FGF-8 and FGFR-3c. We conclude that FGF-8 and its receptors are expressed in preantral follicles in a developmentally regulated manner. (C) 2005 Wiley-Liss, Inc.

Expression and function of fibroblast growth factor 10 and its receptor, fibroblast growth factor receptor 213, in bovine follicles

Buratini, J.; Pinto, M. G. L.; Castilho, A. C.; Amorim, R. L.; Giometti, I. C.; Portela, V. M.; Nicola, E. S.; Price, C. A.
Fonte: Soc Study Reproduction Publicador: Soc Study Reproduction
Tipo: Artigo de Revista Científica Formato: 743-750
Português
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Some fibroblast growth factors (FGFs) affect ovarian follicle cell growth and/or differentiation. Whereas many FGFs activate several FGF receptors, FGF7 and FGF10 primarily activate only one, FGFR2B. As FGF7 is produced by bovine theca cells and acts on granulosa cells, we tested the hypothesis that FGF10 may also play a role in folliculogenesis in cattle. Reverse transcription-polymerase chain reaction demonstrated the presence of FGF10 mRNA in the oocytes and theca cells of the antral follicles, as well as in the preantral follicles. FGF10 protein was detected by immunohistochemistry in the oocytes of the preantral and antral follicles, and in the granulosa and theca cells of the antral follicles. FGF10 expression in theca cells changed during follicle development; mRNA abundance decreased with increasing follicular estradiol concentration in healthy follicles, and was lowest in highly atretic follicles. Culturing of granulosa cells in serum-free medium revealed FSH regulation of FGF10 receptor expression. The addition of FGF10 to cultured granulosa cells decreased the level of estradiol but did not alter cell proliferation. These data support a role for FGF10 in signaling to granulosa cells from theca cells and/or the oocyte.

Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA.

Silvério, Karina G; Martinez, Aurora E T; Rossa Jr., Carlos
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 213-220
Português
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A study was conducted to evaluate in vitro the effect of root surface conditioning with basic fibroblast growth factor (b-FGF) on morphology and proliferation of fibroblasts. Three experimental groups were used: non-treated, and treated with 50 microg or 125 microg b-FGF/ml. The dentin samples in each group were divided into subgroups according to the chemical treatment received before application of b-FGF: none, or conditioned with tetracycline-HCl or EDTA. After contact with b-FGF for 5 min, the samples were incubated for 24 h with 1 ml of culture medium containing 1 x 10(5) cells/ml plus 1 ml of culture medium alone. The samples were then subjected to routine preparation for SEM, and random fields were photographed. Three calibrated and blind examiners performed the assessment of morphology and density according to two index systems. Classification and regression trees indicated that the root surfaces treated with 125 microg b-FGF and previously conditioned with tetracycline-HCl or EDTA presented a morphology more suggestive of cellular adhesion and viability (P = 0.004). The density of fibroblasts on samples previously conditioned with EDTA, regardless of treatment with b-FGF, was significantly higher than in the other groups (P < 0.001). The present findings suggest that topical application of b-FGF has a positive influence on both the density and morphology of fibroblasts.

Reconstruction of abdominal wall defects using small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor

Wang,Liang; Lai,Dong-ming; Yang,Bin; Jiang,Zhi-peng; Zhang,Yu-chao; Zhou,Jun; Lai,Wei; Chen,Shuang
Fonte: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia Publicador: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2014 Português
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PURPOSE: To construct a new biomaterial-small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor, and to evaluate the new biomaterials for the reconstruction of abdominal wall defects. METHODS: Thirty six Sprague-Dawley rats were used in the animal experiments and randomly divided into three groups. The new biomaterial was constructed by combining small intestinal submucosa with gelatin hydrogel for basic fibroblast growth factor release. Abdominal wall defects were created in rats, and repaired using the new biomaterials (group B), compared with small intestinal submucosa (group S) and ULTRAPROTM mesh (group P). Six rats in each group were sacrificed at three and eight weeks postoperatively to examine the gross effects, inflammatory responses, collagen deposition and neovascularization. RESULTS: After implantation, mild adhesion was caused in groups B and S. Group B promoted more neovascularization than group S at three weeks after implantation, and induced significantly more amount of collagen deposition and better collagen organization than groups S and P at eight weeks after implantation. CONCLUSION: Small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor could promote better regeneration and remodeling of host tissues for the reconstruction of abdominal wall defects.

A unique point mutation in the fibroblast growth factor receptor 3 gene (FGFR3) defines a new craniosynostosis syndrome

Muenke, M.; Gripp, K.; McDonald-McGinn, D.; Gaudenz, K.; Whitaker, L.; Bartlett, S.; Markowitz, R.; Robin, N.; Nwokoro, N.; Mulvihill, J.; Losken, H.; Mulliken, J.; Guttmacher, A.; Wilroy, R.; Clarke, L.; Hollway, G.; Ades, L.; Haan, E.; Mulley, J.; Cohen
Fonte: UNIV CHICAGO PRESS Publicador: UNIV CHICAGO PRESS
Tipo: Artigo de Revista Científica
Publicado em //1997 Português
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The underlying basis of many forms of syndromic craniosynostosis has been defined on a molecular level. However, many patients with familial or sporadic craniosynostosis do not have the classical findings of those craniosynostosis syndromes. Here we present 61 individuals from 20 unrelated families where coronal synostosis is due to an amino acid substitution (Pro250Arg) that results from a single point mutation in the fibroblast growth factor receptor 3 gene on chromosome 4p. In this instance, a new clinical syndrome is being defined on the basis of the molecular finding. In addition to the skull findings, some patients had abnormalities on radiographs of hands and feet, including thimble-like middle phalanges, coned epiphyses, and carpal and tarsal fusions. Brachydactyly was seen in some cases; none had clinically significant syndactyly or deviation of the great toe. Sensorineural hearing loss was present in some, and developmental delay was seen in a minority. While the radiological findings of hands and feet can be very helpful in diagnosing this syndrome, it is not in all cases clearly distinguishable on a clinical basis from other craniosynostosis syndromes. Therefore, this mutation should be tested for in patients with coronal synostosis.; M. Muenke...

Fibroblast growth factor homologous factor 2 (FHF2): gene structure, expression and mapping to the Börjeson-Forssman-Lehmann syndrome region in Xq26 delineated by a duplication breakpoint in a BFLS-like patient; Fibroblast growth factor homologous factor 2 (FHF2): gene structure, expression and mapping to the Borjeson-Forssman-Lehmann syndrome region in Xq26 delineated by a duplication breakpoint in a BFLS-like patient

Gecz, J.; Baker, E.; Donnelly, A.; Ming, J.; McDonald-McGinn, D.; Spinner, N.; Zackai, E.; Sutherland, G.; Mulley, J.
Fonte: SPRINGER VERLAG Publicador: SPRINGER VERLAG
Tipo: Artigo de Revista Científica
Publicado em //1999 Português
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Börjeson-Forssman-Lehmann syndrome (BFLS) is a syndromal X-linked mental retardation, which maps by linkage to the q26 region of the human X chromosome. We have identified a male patient with BFLS-like features and a duplication, 46,Y,dup(X)(q26q28), inherited from his phenotypically normal mother. Fluorescence in situ hybridisation using yeast artificial chromosome clones from Xq26 localised the duplication breakpoint to an ∼400-kb interval in the Xq26.3 region between DXS155 and DXS294/DXS730. Database searches and analysis of available genomic DNA sequence from the region revealed the presence of the fibroblast growth factor homologous factor gene, FHF2, within the duplication breakpoint interval. The gene structure of FHF2 was determined and two new exons were identified, including a new 5′ end exon, 1B. FHF2 is a large gene extending over ∼200 kb in Xq26.3 and is composed of at least seven exons. It shows tissue-specific alternative splicing and alternative transcription starts. Northern blot hybridisation showed highest expression in brain and skeletal muscle. The FHF2 gene localisation and tissue-specific expression pattern suggest it to be a candidate gene for familial cases of the BFLS syndrome and other syndromal and non-specific forms of X-linked mental retardation mapping to the region.; Jozef Gecz...

The structure and function of vertebrate fibroblast growth factor receptor 1

Groth, C.; Lardelli, M.
Fonte: Univ Basque Country Press Publicador: Univ Basque Country Press
Tipo: Artigo de Revista Científica
Publicado em //2002 Português
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The vertebrate fibroblast growth factor receptor 1 (FGFR1) is alternatively spliced generating multiple splice variants that are differentially expressed during embryo development and in the adult body. The restricted expression patterns of FGFR1 isoforms, together with differential expression and binding of specific ligands, leads to activation of common FGFR1 signal transduction pathways, but may result in distinctively different biological responses as a result of differences in cellular context. FGFR1 isoforms are also present in the nucleus in complex with various fibroblast growth factors where they function to regulate transcription of target genes.; Casper Groth and Michael Lardelli

The role of the tetraspanin CD151 in primary keratinocyte and fibroblast functions: Implications for wound healing

Geary, S.; Cowin, A.; Copeland, B.; Baleato, R.; Miyazaki, K.; Ashman, L.
Fonte: Academic Press Inc Elsevier Science Publicador: Academic Press Inc Elsevier Science
Tipo: Artigo de Revista Científica
Publicado em //2008 Português
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Previous studies showed that CD151-null mice have a skin wound healing deficit. To gain an understanding of the role of CD151 in re-epithelialisation and dermal contraction, keratinocyte and fibroblast functions were assayed. Primary CD151-null keratinocytes displayed defective migration on Matrigel (a basement membrane equivalent) and laminin-332, the primary adhesion component of basement membranes, but not on collagen-I. Adhesion, spreading and proliferation were also deficient on laminin-332, but not collagen-I. The data suggest that loss of CD151 impairs the function of its primary interaction partners, integrin alpha3beta1- and/or alpha6beta4 which bind to laminin-332. Skin fibroblasts also produce CD151 mRNA. CD151-null fibroblasts migrated significantly faster on collagen I than wild type fibroblasts, confirming that they possess functional collagen receptors. However, no significant decrease in the ability of CD151-null fibroblasts to cause contraction in floating collagen gel assays in response to transforming growth factor beta-1 (TGF-beta1) or platelet derived growth factor (PDGF-BB) was observed, nor was there an effect on fibroblast adhesion or proliferation on collagen-I. The data implicate CD151 as a facilitator of laminin-332-mediated keratinocyte functions that impact on the re-epithelialisation process intrinsic to wound healing and further suggest a potential novel role for CD151 in fibroblast migration.; Sean M. Geary...

Einfluss von thrombocytären Wachstumsfaktoren, einer Keimsuspension mit S. Aureus und Antiproteasen auf das Wachstumsverhalten von Fibroblasten in der Zellkultur; Influence of platelet growth factors, of a bacterial suspension containing S. aureus and antiproteases on the growing of fibroblast cells in cell culture

Holl, Patrick Michael Enno
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
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Chronische Wunden sind seit langem Objekt klinischer Forschung und zahlreicher Studien. Autologe thombozytäre Wachsumtsfaktoren werden zur Behandlung chronischer Wunden eingesetzt, ihre Wirkung wurde in Studien belegt. Bei einem Teil der behandelten Patienten konnte jedoch keine Wirkung festgestellt werden. Ziel dieser Studie war die Erforschung des Einflusses von autologen thrombozytären Wachstumsfaktoren, bakteriellen Proteasen in einer definierten Keimlösung , definierten Antiproteasen auf in vitro kultivierte humane Fibroblasten. Es zeigte sich ein signifikanter positiver Effekt von thrombozytären Wachstumsfaktoren auf die Fibroblastenkultur. In einer Konzentration von 1/20 zum Standardmedium zugegeben stieg die Zellzahl der Kultur nach 1 Woche auf das 2,5 fache der Kontrollkultur. In einer Konzentration von 1/10 zugegeben führten die Wachstumsfaktoren zu einem 3,5 fachem Anstieg. Untersuchtes Wundexsudat einer chronischen Wunde zeigte unter sterilen Bedingungen keine signifikante Wirkung auf die Vermehrung der Fibroblasten. Definierte Keimlösungen des Keims Staphyloccocus aureus ATC25322 zeigten, 1 /10 zum Standardmedium zugegeben einen signifikant negativen Einfluss auf die Zellzahl der Kultur nach 1 Woche, sowohl bei der Kontrollkultur als auch bei mit Wachstumsfaktoren angezüchteten Kulturen. Aprotinin hatte...

Thrombozytäres bFGF (basic fibroblast growth factor) vermittelt endothelintegrative Funktionen von mesenchymalen Stammzellen in vitro; Platelet derived bFGF mediates vascular integrative mechanisms ofmesenchymal stem cells in vitro

Froihofer, Claudia Amrei
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
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36.54%
Herzinfarktpatienten zeigen eine vermehrte Anzahl zirkulierender mesenchymaler Stammzellen (MSCs), die bekanntermaßen Myokard regenerieren und zu Endothelzellen differenzieren können. Die Mechanismen der MSCs um aus der Zirkulation heraus das Zielgewebe zu erreichen (sog. homing), sind weiterhin unbekannt. In dieser Arbeit wurde die Auswirkung der Thrombozyten auf die Rekrutierung der MSCs, deren Proliferation, deren Migration und deren Integration ins Endothel untersucht. Mesenchymale Stammzellen, die das Alpha-v-Beta-3-Integrin exprimieren, wurden bei hohen Scherkräften an humane arterielle Endothelzellen vermittelt, nachdem diese mit isolierten Thrombozyten oder IL-1-Beta behandelt worden waren. Die Interaktion von Thrombozyten mit MSCs wurde durch Präinkubation mit dem Beta-3-blockierenden monoklonalen Antikörper 7E3 oder RGD, jedoch nicht mit RED, verhindert. Des Weiteren förderten Thrombozyten einen Migrationszelltyp der MSCs und verstärkten dosis- und aktivierungsabhängig die Migration der MSCs. Dieser Vorgang wurde über bFGF (basic fibroblast growth factor) vermittelt. Vergleichbar dazu induzierte thrombozytäres bFGF eine MSC-Proliferation. Die Koinkubation von MSCs mit Thrombozyten förderte die Integration in einen endothelialen Monolayer...

Fibroblast growth factor 17 and bone morphogenetic protein 15 enhance cumulus expansion and improve quality of in vitro-produced embryos in cattle

Machado, Mariana Fernandes; Caixeta, Ester Siqueira; Sudiman, Jaqueline; Gilchrist, Robert B.; Thompson, Jeremy G.; Lima, Paula Fernanda; Price, Christopher A.; Buratini, Jose
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 390-398
Português
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Processo FAPESP: 2009/51725-0; Bone morphogenetic protein 15 (BMP15) and members of the fibroblast growth factor (FGF) family are expressed by the oocyte and are involved in the control of cumulus cell function. We tested the hypothesis that FGF17, alone or combined with BMP15 in the maturation medium, enhances cumulus expansion, meiosis progression, embryonic development, and expression of mRNA encoding key genes regulating expansion (prostaglandin-endoperoxide synthase 2 [PTGS2], hyaluronan synthase 2 [HAS2], tumor necrosis factor-stimulated gene 6 [TNFAIP6], and pentraxin 3 [PTX3]) and markers of oocyte developmental competence (phosphofructokinase [PFKP], gremlin [GREM1], versican [VCAN], and the genomic progesterone receptor [nPR]) in cumulus cells. Fibroblast growth factor 17 and BMP15 increased the percentage of fully expanded cumulus-oocyte complexes (COCs), but there was no additive effect when both were combined. Neither FGF17 nor BMP15 altered the percentage of oocytes reaching meiosis II at the end of COC culture or cleavage and blastocyst rates after IVF. However, embryo quality, as assessed by the number of cells in the inner cell mass...

Expressão hipocampal de fatores de crescimento de fibroblastos em pacientes com epilepsia do lobo temporal= : Hippocampal expression of fibroblast growth factors in temporal lobe epilepsy patients; Hippocampal expression of fibroblast growth factors in temporal lobe epilepsy patients

Ana Erika Dias Ferreira
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 25/08/2014 Português
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Epilepsia do lobo temporal (ELT) é a forma mais comum de epilepsia em adultos. O processo de epileptogênese inclui a morte neuronal, brotamento axonal, inflamação, neurogênese, estresse oxidativo e gliose. No entanto, os mecanismos moleculares subjacentes não são totalmente compreendidos. Os fatores de crescimento de fibroblastos (FGFs) são uma família de proteínas com várias funções no organismo, especialmente no sistema nervoso central. No entanto, o funcionamento dos FGFs no cérebro humano não é totalmente compreendido. O FGF2 é o membro mais estudado dessa família e seu papel na fisiopatologia da epilepsia é controversa. Na tentativa de esclarecer o envolvimento da via de FGF na ELT, nós quantificamos a expressão hipocampal dos seguintes genes: FGF2, FGF8, FGF22, FGFR1, FGFR2, FGFR3, ITPR3, PIK3R3 e PIK3R5 em 10 pacientes resistentes a fármacos e quatro controles post mortem. Além disso, avaliamos a expressão da proteína de FGF2 por imunofluorescência indireta. Apenas para o FGF2, houve aumento do RNAm no hipocampo dos pacientes para os dois genes de referência testados, HPRT1 e ENO2 + TBP em combinação (P = 0,002 e P = 0,036; respectivamente). A porcentagem de células imunomarcadas para FGF2 no giro dentado foi maior nos pacientes do que nos controles (P <0...

FGF-2, IL-1β and TFG-β regulate fibroblast expression of S100A8

Rahimi, Ahmed (Farid); Hsu, Kenneth; Endoh, Yasumi; Geczy, Carolyn
Fonte: Blackwell Publishing Ltd Publicador: Blackwell Publishing Ltd
Tipo: Artigo de Revista Científica
Português
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Growth factors, including fibroblast growth factor-2 (FGF-2) and transforming growth factor-β (TGF-β) regulate fibroblast function, differentiation and proliferation. S100A8 and S100A9 are members of the S100 family of Ca 2+-binding proteins and are now

Desenvolvimento exoeritrocítico de Plasmodium gallinaceum em cultura primária de fibroblasto de embrião de galinha; Exoerythrocytic development of Plasmodium gallinaceum in primary fibroblast culture of chicken embryo

Couto-Lima, Dinair; Lourenço-de-Oliveira, Ricardo; Meirelles, Maria de Nazareth Leal de; Porrozzi, Renato
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; ; Formato: application/pdf
Publicado em 01/01/2008 Português
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No presente estudo, avaliamos a susceptibilidade de cultura primária de fibroblastos de embrião de galinha à infecção por esporozoítas de P. gallinaceum, assim como o desenvolvimento de estágios do ciclo exoeritrocítico. Fibroblastos foram obtidos a partir da musculatura do peito de embriões de galinha e esporozoítas foram obtidos de glândulas salivares de Aedes fluviatilis experimentalmente infectados. Após períodos de 1h, 3h, 24h, 48h e 72h após a infecção, culturas de células foram fixadas e analisadas através de imunofluorescência indireta empregando-se anticorpos monoclonais contra a proteína circum-esporozoíta e microscopia eletrônica de transmissão. Proteína circum-esporozoíta foi detectada em todas as formas parasitárias. O percentual médio de fibroblastos com esporozoítas aderidos ou já penetrados não aumentou proporcionalmente com a concentração de parasitos no inóculo e independeu se o soro utilizado no cultivo celular era soro bovino fetal ou soro de galinha normal. Foi observado que, quando maior é o período de incubação, maior é a possibilidade dos esporozoítas aderirem e penetrarem nos fibroblastos. Esporozoítas foram observados penetrando em fibroblastos depois de 3h de incubação...