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Endurance training induces depot-specific changes in IL-10/TNF-alpha ratio in rat adipose tissue

LIRA, F. S.; ROSA, J. C.; YAMASHITA, A. S.; KOYAMA, C. H.; BATISTA JR., M. L.; SEELAENDER, M.
Fonte: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD Publicador: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
Tipo: Artigo de Revista Científica
Português
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White adipose tissue (WAT) is the source of pro- and anti-inflammatory cytokines and recently, it has been recognized as an important source of interleukin 10 (IL-10). Acute physical exercise is known to induce an anti-inflammatory cytokine profile, however, the effect of chronic physical exercise on the production of IL-10 by WAT has never been examined. We assessed IL-10 and TNF-alpha concentration in WAT of rats engaged in endurance training. Animals were randomly assigned to either a sedentary control group (S, n = 7) or an endurance trained group (T, n = 8). Trained rats ran on a treadmill 5 days/wk for 8 wk (55-65% VO(2max). Detection of IL-10 and TNF-alpha protein and mRNA expression, as well as the gene expression of PPAR-gamma, and immunocytochemistry to detect mononuclear phagocytes were carried out. A reduction in absolute retroperitoneal adipose tissue (RPAT) weight in T (44%; p < 0.01), when compared with S was observed. IL-10 concentration was increased (1.5-fold, p < 0.05), to a higher extent than that of TNF-alpha (66%. p < 0.05) in the mesenteric adipose tissue (MEAT) of the trained group, while no change related to training was observed in RPAT. In MEAT, IL-10/TNF-alpha ratio was increased in T, when compared with S (30%; p < 0.05). PPAR-gamma gene expression was increased in T (1.1-fold; p < 0.01)...

Early IL-10 production is essential for syngeneic graft acceptance

Takiishi, Tatiana; Tadokoro, Carlos Eduardo; Rizzo, Luiz Vicente; de Moraes, Luciana Vieira
Fonte: FEDERATION AMER SOC EXP BIOL; BETHESDA Publicador: FEDERATION AMER SOC EXP BIOL; BETHESDA
Tipo: Artigo de Revista Científica
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We performed a comparative study and evaluated cellular infiltrates and anti-inflammatory cytokine production at different time-points after syngeneic or allogeneic skin transplantation. We observed an early IL-10 production in syngeneic grafts compared with allografts. This observation prompted us to investigate the role of IL-10 in isograft acceptance. For this, we used IL-10 KO and WT mice to perform syngeneic transplantation, where IL-10 was absent in the graft or in the recipient. The majority of syngeneic grafts derived from IL-10 KO donors did not engraft or was only partially accepted, whereas IL-10 KO mice transplanted with skin from WT donors accepted the graft. We evaluated IL-10 producers in the transplanted skin and observed that epithelial cells were the major source. Taken together, our data show that production of IL-10 by donor cells, but not by the recipient, is determinant for graft acceptance and strongly suggest that production of this cytokine by keratinocytes immediately upon transplantation is necessary for isograft survival. J. Leukoc. Biol. 92: 259-264; 2012.; FAPESP; FAPESP; CNPq; CNPq; FCT; FCT

Activation of PAF-receptor induces regulatory dendritic cells through PGE2 and IL-10

Koga, Marianna Mainardi; Bizzarro, Bruna; Nunes, Anderson de Sá; Rios, Francisco José Oliveira; Negro, Sonia Jancar
Fonte: Churchill Livingstone; Edinburgh Publicador: Churchill Livingstone; Edinburgh
Tipo: Artigo de Revista Científica
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Activation of the platelet-activating factor receptor (PAFR) in macrophages is associated with suppressor phenotype. Here, we investigated the PAFR in murine dendritic cells (DC). Bone marrow-derived dendritic cells (BALB/c) were cultured with GM-CSF and maturation was induced by LPS. The PAFR antagonists (WEB2086, WEB2170, PCA4248) and the prostaglandin (PG) synthesis inhibitors (indomethacin, nimesulide and NS-398) were added before LPS. Mature and immature DCs expressed PAFR. LPS increased MHCII, CD40, CD80, CD86, CCR7 and induced IL-10, IL-12, COX-2 and PGE2 expression. IL-10, COX-2 and PGE2 levels were reduced by PAFR antagonists and increased by cPAF. The IL-10 production was independent of PGs. Mature DCs induced antigen-specific lymphocyte proliferation. PAFR antagonists or PG-synthesis inhibitors significantly increased lymphocyte proliferation. It is proposed that PAF has a central role in regulatory DC differentiation through potentiation of IL-10 and PGE2 production.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A função da IL-10 na paracoccidioidomise pulmonar murina.; The role of IL-10 in murine pulmonary paracoccidioidomycosis.

Costa, Tânia Alves da
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 08/10/2010 Português
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O principal mecanismo de defesa de hospedeiros infectados pelo Paracoccidioides brasiliensis (Pb), fungo dimórfico que causa a mais importante micose sistêmica da América Latina, é a imunidade celular. Neste processo participam macrófagos ativados por IFN-g e a IL-10 parece ser a citocina que se contrapõe a esta ativação. Tanto na patologia humana como em modelos experimentais há fortes indicações de que a IL-10 age como supressora da imunidade celular causando efeitos deletérios aos hospedeiros; entretanto, estudos diretos sobre a função da IL-10 na paracoccidiodomicose (PCM) não tinham sido ainda realizados. Então o objetivo fundamental deste trabalho foi estudar a função da IL-10 nos mecanismos da imunidade inata e adaptativa contra o Pb utilizando como modelo experimental camundongos geneticamente deficientes de IL-10 (IL-10 nocaute, IL-10 KO) em comparação com seus controles normais (WT). Demonstramos in vitro que macrófagos peritoneais normais de camundongos IL-10 KO apresentam uma maior atividade fagocítica e fungicida que os macrófagos de camundongos WT e isto esteve associado à maior produção de IFN-g, TNF-α, óxido nítrico (NO) e da quimiocina MCP-1. Verificamos...

Associação entre polimorfismos de genes do sistema imunológico (IL-10, TNF-a) e a infecção por HPV nos diferentes graus de lesões cervicais

Igansi, Cristine Nascente
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Tese de Doutorado Formato: application/pdf
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Estudos epidemiológicos e moleculares têm sugerido que o HPV é o principal fator de risco para o desenvolvimento de lesões malignas na cérvice uterina. E, sendo o número de infecções extremamente maior do que o número de casos de câncer cervical, este fato nos leva à investigação de outros fatores associados, como por exemplo, a predisposição imunológica do hospedeiro. Este estudo tem como objetivo avaliar a associação dos polimorfismos (-1082A/G) e (-308 A/G), localizados nos genes da IL-10 e TNF-α, respectivamente, com a infecção genital pelo Papilomavírus Humano (HPV), incluindo os tipos oncogênicos HPV-16, 18 e 31, visto que, estas citocinas são moléculas importantes na resposta imune contra infecções virais. Trata-se de um estudo de casos e controles. O grupo controle foi composto por 211 mulheres, que apresentavam resultado negativo para infecção genital por HPV, identificada através da técnica de Reação em Cadeia da Polimerase (PCR) e exame citopatológico sem alterações. Já os casos, corresponderam a 84 mulheres com infecção genital por HPV e resultado anatomopatológico alterado. A técnica de amplificação refratária de mutações (ARMS-PCR) foi utilizada para a identificação dos polimorfismos. Regressão logística múltipla foi utilizada para verificar a associação das variáveis estudadas com o desfecho (infecção genital pelo HPV).O cálculo de Equilíbrio de Hardy-Weinberg foi utilizado para verificar se as freqüências alélicas e gentotípicas observadas estão de acordo com as esperadas na população em estudo. Para os resultados de IL-10...

Imbalance of IL-2, IFN-gamma and IL-10 secretion in the immunosuppression associated with human paracoccidioidomycosis

Benard, G.; Romano, C. C.; Cacere, C. R.; Juvenale, M.; Mendes Giannini, Maria José Soares; Duarte, A. J. S.
Fonte: W B Saunders Co Publicador: W B Saunders Co
Tipo: Artigo de Revista Científica Formato: 248-252
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Patients with paracoccidioidomycosis (PCM) display a certain degree of immunecompromise characterized by lymphocyte hyporesponsiveness to the main Paracoccidioides brasiliensis antigen (gp43). To determine whether cytokines are involved in this state, we evaluated the secretion of IL-2, IL-10 and IFN-gamma by peripheral blood mononuclear cells (PBMC) from patients with the acute (AF) and chronic (CF) forms of PCM and from healthy, P. brasiliensis-sensitized subjects. gp43-stimulated PBMC from healthy subjects produced substantial amounts of IL-2, IFN-gamma and IL-10, whereas PBMC from AF and CF patients produced low levels of IL-2 and IFN-gamma but substantial amounts of IL-10, Phytohaemagglutinin-induced cytokine secretion was comparable among AF and CF patients and healthy subjects, suggesting integrity of non-specific cellular immune mechanisms in PCM. gp43-pulsed adherent cells, but not non-adherent cells, mere the main source of IL-10, Moreover, IL-2 and IFN-gamma secretion correlated inversely with the amount of specific antibodies produced by patients and healthy subjects. Our results suggest that the imbalance in cytokine production of patients with PCM plays a role in the gp43-hyporesponsiveness and the marked (non-protective) antibody production of these patients. (C) 2001 Academic Press.

CD4+FOXP3+cells produce IL-10 in the spleens of dogs with visceral leishmaniasis

Oliveira Silva, Kathlenn Liezbeth; Andrade, Mariana M. C. de; Melo, Larissa M.; Perosso, Juliana; Vasconcelos, Rosemeri O.; Munari, Danisio P.; Lima, Valeria M. F.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 313-318
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 09/11576-5; Visceral Leishmaniasis (VL) is caused by intracellular parasites of the genus Leishmania that affect humans and several animal species. Dogs are one of the main urban reservoirs of the parasite and play a central role in the transmission cycle to humans via sandflies. Studies concerning the immune response in dogs with VL have demonstrated that protective immunity is associated with cellular immune response, while disease progression is associated with humoral response and IL-10 and TGF-beta production. The study aimed to evaluate IL-10 and TGF-beta production by regulatory T (Treg) cells in the blood and spleen of dogs naturally infected by Leishmania spp. and correlate this with parasite load. Five healthy dogs and 29 dogs with proven infection were selected for the study group. Real-time PCR was used to quantify parasite load and confirm infection by Leishmania spp. Treg cells producing IL-10 and TGF-beta were quantified using flow cytometry. An increase in IL-10 production by Treg cells was verified in the spleen of dogs naturally infected by Leishmania spp. Concurrently, a decrease in the total number of T cells in these dogs was verified compared with healthy dogs. No association was determined between parasite load and the percentage of spleen Treg cells producing IL-10 and TGF-beta. These findings suggest that Treg cells are an important source of IL-10 in the spleen...

Differential post-transcriptional regulation of IL-10 by TLR2 and TLR4-activated macrophages

Rodrigues, Fernando; Pedrosa, Jorge; Coelho, Maria Teixeira; Guedes, Joana; Ferreirinha, Pedro; Howes, Ashleigh; Lai, Wi S.; Blackshear, Perry J.; O´Garra, Anne; Castro, António G.; Saraiva, Margarida
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Artigo de Revista Científica
Publicado em 16/03/2014 Português
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The activation of TLRs by microbial molecules triggers intracellular-signaling cascades and the expression of cytokines such as IL-10. Il10 expression is tightly controlled to ensure effective immune responses, while preventing pathology. Maximal TLR-induction of Il10 transcription in macrophages requires signaling through the MAPKs, ERK, and p38. Signals via p38 downstream of TLR4 activation also regulate IL-10 at the post-transcriptional level, but whether this mechanism operates downstream of other TLRs is not clear. We compared the regulation of IL-10 production in TLR2 and TLR4-stimulated BM-derived macrophages and found different stability profiles for the Il10 mRNA. TLR2 signals promoted a rapid induction and degradation of Il10 mRNA, whereas TLR4 signals protected Il10 mRNA from rapid degradation, due to the activation of Toll/IL-1 receptor domain-containing adaptor inducing IFN-ß (TRIF) and enhanced p38 signaling. This differential post-transcriptional mechanism contributes to a stronger induction of IL-10 secretion via TLR4. Our study provides a molecular mechanism for the differential IL-10 production by TLR2- or TLR4-stimulated BMMs, showing that p38-induced stability is not common to all TLR-signaling pathways. This mechanism is also observed upon bacterial activation of TLR2 or TLR4 in BMMs...

IL-10 release by bovine epithelial cells cultured with Trichomonas vaginalis and Tritrichomonas foetus

Vilela,Ricardo Chaves; Benchimol,Marlene
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2013 Português
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Trichomonas vaginalis and Tritrichomonas foetus are parasitic protists of the human and bovine urogenital tracts, respectively. Several studies have described the cytotoxic effects of trichomonads on urogenital tract epithelial cells. However, little is known about the host cell response against trichomonads. The aim of this study was to determine whether T. foetus and T. vaginalis stimulated the release of the cytokine interleukin (IL)-10 from cultured bovine epithelial cells. To characterise the inflammatory response induced by these parasites, primary cultures of bovine oviduct epithelial cells were exposed to either T. vaginalis or T. foetus. Within 12 h after parasite challenge, supernatants were collected and cytokine production was analysed. Large amounts of IL-10 were detected in the supernatants of cultures that had been stimulated with T. foetus. Interestingly, T. vaginalis induced only a small increase in the release of IL-10 upon exposure to the same bovine cells. Thus, the inflammatory response of the host cell is species-specific. Only T. foetus and not T. vaginalis induced the release of IL-10 by bovine oviduct epithelial cells.

IL-10 na patogênese da infecção por Leishmania infantum

Monteiro, Glória Regina de Góis
Fonte: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Ciências da Saúde; Ciências da Saúde Publicador: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Ciências da Saúde; Ciências da Saúde
Tipo: Tese de Doutorado Formato: application/pdf
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Conselho Nacional de Desenvolvimento Científico e Tecnológico; Leishmaniose visceral (LV) é uma doença, potencialmente, fatal cujo agente etiológico nas Américas é a espécie Leishmania infantum. No Brasil, a LV era uma doença predominantemente rural até os anos 80, quando epidemias passaram a ocorrer nas grandes cidades, devido à migração da população para periferia dos centros urbanos, especialmente, na região nordeste. A infecção por leishmania pode evoluir com um amplo espectro clínico que é dependente da resposta imune desenvolvida pelo hospedeiro. Notadamente, 80% a 90% da infecção humana é subclínica ou assintomática, mas os fatores que determinam a evolução para doença não são completamente esclarecidos. O controle da infecção por leishmania é dependente da imunidade celular mediada por IFN-. Está demonstrado que a leishmania suprime a resposta microbicida do macrófago. Estudos prévios sugerem que o perfil genético do hospedeiro contribui para a evolução clínica após a infecção. O objetivo deste estudo foi avaliar o papel da Interleucina 10 na patogênese da LV e avaliar a expressão de genes envolvidos na resposta imune, frente à infecção por L. infantum. Foram avaliados pessoas com LV...

Efeito do bloqueio do receptor de IL-10 na resposta imune contra Leishmania amazonensis.

Amaral, Joana Ferreira do
Fonte: Programa de P?s-Gradua??o em Ci?ncias Biol?gicas. N?cleo de Pesquisas em Ci?ncias Biol?gicas, Pr?-Reitoria de Pesquisa e P?s Gradua??o, Universidade Federal de Ouro Preto. Publicador: Programa de P?s-Gradua??o em Ci?ncias Biol?gicas. N?cleo de Pesquisas em Ci?ncias Biol?gicas, Pr?-Reitoria de Pesquisa e P?s Gradua??o, Universidade Federal de Ouro Preto.
Tipo: Dissertação
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A avalia??o do papel da IL-10 na leishmaniose ? de extrema import?ncia, pois sendo uma citocina anti-inflamat?ria, inibe a a??o de IFN-ge NO, que t?m papel importante na elimina??o do parasita e no controle da les?o. O papel da IL-10na infec??o por L.(L.) amazonensis foi avaliado in vivo neste trabalho tratando-se camundongos C57BL/6, suscet?veis ? essa esp?cie de Leishmania, comanticorpos monoclonais anti-receptor de IL-10(a-IL-10R) na fase inicial da infec??o. Como controle de tratamento inoculamos um grupo de animais com IgG total de rato na mesma dose usada para o anticorpoa-IL-10R(0,5mg/animal/dose) e mantivemos outro grupo sem nenhum tratamento. Os animais tratados coma-IL-10R, ap?s 3semanas de infec??o, apresentaram perfil histopatol?gico, produ??odeTNFequantifica??odeparasitas semelhanteaos grupos controle, com exce??o da produ??o de IFN-g produzido por c?lulas mononucleares de linfonodo de camundongos tratados com a-IL-10R, que apresentaram quantidades dessa citocina superiores aos animais controles. Em rela??o aos animais sacrificados 12 semanas ap?s a infec??o, o curso da infec??o, o parasitismo e a produ??o de citocinas foram semelhantes para todos os grupos. Em rela??o ? an?lise histol?gica, observamos um maior infiltrado inflamat?rio e um parasitismo tecidual ligeiramente mais acentuado no grupo tratado coma-IL-10 R em rela??o aos controles. Dessa forma...

Influência das citocinas IL-10, TGF-β1 e IFN-γ na susceptibilidade de células trofoblásticas humanas (linhagem BeWo) e células epiteliais uterinas humanas (linhagem HeLa) à infecção por Toxoplasma gondii: papel na expressão de ICAM-1, na adesão do parasito à célula hospedeira e vias de sinalização intracelulares ativadas

Barbosa, Bellisa de Freitas
Fonte: Universidade Federal de Uberlândia Publicador: Universidade Federal de Uberlândia
Tipo: Tese de Doutorado
Português
Relevância na Pesquisa
66.07%
Toxoplasma gondii é um protozoário parasito intracelular obrigatório capaz de infectar uma diversidade de células, incluindo células trofoblásticas. Estudos prévios demonstraram que interleucina (IL)-10, fator transformador de crescimento (TGF)-β1 e interferon (IFN)-γ são importantes citocinas envolvidas na susceptibilidade de células trofoblásticas humanas (linhagem BeWo) e células epiteliais uterinas humanas (linhagem HeLa) à infecção por T. gondii. Adicionalmente, T. gondii é capaz de aderir à membrana plasmática de células hospedeiras através da molécula de adesão intercelular tipo 1 (ICAM-1). O presente estudo investigou dois objetivos gerais: (i) verificar o papel de IL-10, TGF-β1 e IFN-γ na expressão de ICAM-1 em células BeWo e HeLa, bem como analisar a influência de ICAM-1 na adesão de T. gondii a estas células quando tratadas ou não com as mesmas citocinas; e (ii) verificar os mecanismos intracelulares ativados por IL-10, TGF-β1 e IFN-γ em células BeWo e HeLa no intuito de compreender os efeitos diferenciais exercidos por essas citocinas nessas distintas linhagens celulares. Para executar o primeiro objetivo, células BeWo e HeLa foram infectadas por T. gondii (cepa RH) e tratadas com rIL-10...

Asociación de polimorfismos genéticos de TNF-α e IL-10, citocinas reguladoras de la respuesta inmune en enfermedade; Asociación Colombiana de Infectología

CASAS, LILIAN ANDREA; GOMEZ, ALBERTO
Fonte: Pontifícia Universidade Javeriana Publicador: Pontifícia Universidade Javeriana
Formato: 38 - 53
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12 - 1; Resumen 1 Bacterióloga, joven investigadora, Colciencias, 2007. 2 Microbiólogo, Ph.D. Profesor Titular, Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia El TNF-α y la IL-10 son citocinas reguladoras que intervienen en los procesos inmunológicos y actúan como mediadores proinflamatorios y antiinflamatorios, respectivamente. Se ha demostrado que los niveles de estos mediadores inciden en la susceptibilidad y en el curso de diferentes enfermedades infecciosas, alérgicas y autoinmunes. Se ha reportado un importante número de polimorfismos en la región promotora del gen correspondiente y algunos de ellos se han relacionado directamente con los niveles de expresión y producción de estas citocinas, en particular en los nucleótidos de las posiciones -308 del gen de TNF-α y -1082, -819, -592 del gen de IL-10. Varios estudios han demostrado asociación de estos polimorfismos simples (SNP, single nucleotide polymorphisms) con la susceptibilidad y severidad de este tipo de enfermedades. Este artículo de revisión presenta la síntesis de la relación de estos polimorfismos genéticos con la susceptibilidad y el curso de las enfermedades infecciosas, alérgicas y autoinmunes.; Abstract TNF-α and IL-10 are regulatory cytokines that participate in the immune response acting either as pro-inflammatory or as anti-inflammatory mediators...

Avaliação da expressão salivar e tecidual das citocinas TGF-β e IL-10 em pacientes com carcinoma espinocelular de cavidade oral; Evaluation of saliva and tissue expression of TGF-β and IL-10 in patients with oral squamous cell carcinoma

Arantes, Diego Antonio Costa
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Odontologia (FO); Faculdade de Odontologia - FO (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Odontologia (FO); Faculdade de Odontologia - FO (RG)
Tipo: Dissertação Formato: application/pdf
Português
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The transforming growth factor β (TGF-β) and interleukin 10 (IL-10) are immunosuppressive cytokines which promote failure of the local anti-tumor immune response and, therefore, influence the proliferation and prognosis of malignant neoplasms. The aim of this study was to investigate the tissue and salivary expression of TGF-β and IL-10 in patients with oral squamous cell carcinoma (OSCC) and compare it with that of healthy subjects (Control). The association of these cytokines with clinical parameters of prognosis (staging, metastasis and survival) and histological grade of malignancy (WHO grading) was also investigated. Cytokines in the tissue (OSCC, n = 65; Control, n = 30) were identified using the immunohistochemistry technique (IHC) and in the saliva (OSCC, n = 22; Control, n = 23) the Enzyme-linked Immunosorbent Assay (ELISA) was used. The tissue expression of TGF-β and IL-10 in metastatic lymph nodes (n = 23) of OSCC patients was investigated. The expression of TGF-β and IL-10 in the tissue was measured using a semi-quantitative method in conjunction with staining intensity. Our findings demonstrated a high tissue expression of IL-10 and TGF-β2, and a low or absent expression of TGF-β1, in the majority of OSCC samples when compared to the group with clinically healthy oral mucosa (Control) (p < 0.05 for IL-10 and TGF-β2). The salivary concentration of IL-10 was also high...

IL-10 Inhibits the NF-kB and ERK/MAPK-Mediated Production of Pro-Inflammatory Mediators by UpRegulation of SOCS-3 in Trypanosoma cruzi-Infected Cardiomyocytes

Hovsepian, Eugenia; Penas, Federico Nicolás; Siffo, Sofía; Mirkin Gerardo A.; Goren, Nora Beatriz
Fonte: Public Library Science Publicador: Public Library Science
Tipo: info:eu-repo/semantics/article; info:ar-repo/semantics/artículo; info:eu-repo/semantics/publishedVersion Formato: application/pdf
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Trypanosoma cruzi (T. cruzi) infection produces an intense inflammatory response which is critical for the control of the evolution of Chagas´ disease. Interleukin (IL)-10 is one of the most important anti-inflammatory cytokines identified as modulator of the inflammatory reaction. This work shows that exogenous addition of IL-10 inhibited ERK1/2 and NF-κB activation and reduced inducible nitric oxide synthase (NOS2), metalloprotease (MMP) -9 and MMP-2 expression and activities, as well as tumour necrosis factor (TNF)-α and interleukin (IL)-6 expression, in T. cruzi-infected cardiomyocytes. We found that T. cruzi and IL-10 promote STAT3 phosphorylation and up-regulate the expression of suppressor of cytokine signalling (SOCS)-3 thereby preventing NF-κB nuclear translocation and ERK1/2 phosphorylation. Specific knockdown of SOCS-3 by small interfering RNA (siRNA) impeded the IL-10-mediated inhibition of NF-κB and ERK1/2 activation. As a result, the levels of studied pro-inflammatory mediators were restored in infected cardiomyocytes. Our study reports the first evidence that T. cruzi up- regulates SOCS-3 expression and highlights the relevance of IL-10 in the modulation of pro-inflammatory response of cardiomyocytes in Chagas´ disease.; Fil: Hovsepian...

Estudio de los efectos antiinflamatorios de 15-deoxi-delta 12,14-prostaglandina J2 e IL-10 en miocardiocitos infectados con Trypanosoma cruzi. Mecanismos moleculares involucrados; Study of the antiinflammatory effects of 15-deoxy-delta 12,14-prostaglandin J2 and IL-10 in Trypanosoma cruzi infected cardiomyocytes. Molecular mechanisms involved

Hovsepian, Eugenia
Fonte: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires Publicador: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires
Tipo: info:eu-repo/semantics/doctoralThesis; tesis doctoral; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em //2012 Português
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En la fase aguda de la enfermedad de Chagas, la infección por T. cruzi produce una intensa respuesta inflamatoria en diversos tejidos del organismo incluso en el corazón, donde los miocardiocitos constituyen un importante blanco. Si bien la reacción inflamatoria es importante para el control inicial de la infección, un desequilibrio o una mala resolución de la misma puede generar daños y alteraciones en la funcionalidad cardíaca. Las células cardíacas responden a la infección mediante la expresión de enzimas inflamatorias como óxido nítrico sintasa 2 (NOS2) y metaloproteasas (MMPs) y citoquinas como TNF-α e IL-6. Esta respuesta está mediada por la activación de vías de señalización que incluyen al factor de transcripción nuclear-ĸB (NF-ĸB) y a las quinasas reguladas por señales extracelulares-proteínas quinasas activadas por mitógenos (ERK1/2-MAPK), cuya participación es fundamental en procesos inflamatorios. Los receptores activados por factores de proliferación peroxisomal γ (PPARγ) son factores de transcripción dependientes de ligando que han sido implicados en la regulación del metabolismo lipídico y de procesos inflamatorios. En este trabajo evaluamos el aporte de 15-deoxi-Δ12,14-prostaglandina J2 (15dPGJ2)...

Estudio de los efectos antiinflamatorios de 15-deoxi-delta 12,14-prostaglandina J2 e IL-10 en miocardiocitos infectados con Trypanosoma cruzi. Mecanismos moleculares involucrados; Study of the antiinflammatory effects of 15-deoxy-delta 12,14-prostaglandin J2 and IL-10 in Trypanosoma cruzi infected cardiomyocytes. Molecular mechanisms involved

Hovsepian, Eugenia
Fonte: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires Publicador: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires
Tipo: Tesis Doctoral Formato: text; pdf
Publicado em //2012 Português
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66.05%
En la fase aguda de la enfermedad de Chagas, la infección por T. cruzi produce una intensa respuesta inflamatoria en diversos tejidos del organismo incluso en el corazón, donde los miocardiocitos constituyen un importante blanco. Si bien la reacción inflamatoria es importante para el control inicial de la infección, un desequilibrio o una mala resolución de la misma puede generar daños y alteraciones en la funcionalidad cardíaca. Las células cardíacas responden a la infección mediante la expresión de enzimas inflamatorias como óxido nítrico sintasa 2 (NOS2) y metaloproteasas (MMPs) y citoquinas como TNF-α e IL-6. Esta respuesta está mediada por la activación de vías de señalización que incluyen al factor de transcripción nuclear-ĸB (NF-ĸB) y a las quinasas reguladas por señales extracelulares-proteínas quinasas activadas por mitógenos (ERK1/2-MAPK), cuya participación es fundamental en procesos inflamatorios. Los receptores activados por factores de proliferación peroxisomal γ (PPARγ) son factores de transcripción dependientes de ligando que han sido implicados en la regulación del metabolismo lipídico y de procesos inflamatorios. En este trabajo evaluamos el aporte de 15-deoxi-Δ12,14-prostaglandina J2 (15dPGJ2)...

IL-10 and TNF-α polymorphisms in subjects with irritable bowel syndrome in Mexico

Schmulson,Max; Pulido-London,Daniela; Rodríguez,Óscar; Morales-Rochlin,Norma; Martínez-García,Rosalinda; Gutiérrez-Ruiz,María Concepción; López-Alvarenga,Juan Carlos; Gutiérrez-Reyes,Gabriela
Fonte: Revista Española de Enfermedades Digestivas Publicador: Revista Española de Enfermedades Digestivas
Tipo: info:eu-repo/semantics/article; journal article; info:eu-repo/semantics/publishedVersion Formato: text/html; application/pdf
Publicado em 01/08/2013 Português
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65.98%
Background: there has been recent evidence of an alteration in irritable bowel syndrome (IBS) immune regulation, as well as variations in cytokine polymorphisms. Aims: to determine the frequency of the IL-10 (-1082G/A) and TNF-α (-308G/A) polymorphisms in subjects with IBS in Mexico. Methods: volunteers answered the Rome II Questionnaire and were classified as IBS (n = 45) and controls (n = 92). The IBS subjects were then categorized as IBS-D: 22.2 %, IBS-C: 28.9 %, and IBS-A/M: 48.9 %. The polymorphism frequency among groups was compared. Results: there were no differences between IBS vs. controls in the frequency of the high (8.9 vs. 18.5 %), intermediate (60.0 vs. 57.6 %), or low (23.9 vs. 38.9 %) producer IL-10 genotypes, p = 0.315. Neither were there differences in the high (0 vs. 1.1 %), intermediate (55.4 vs. 43.2 %), or Low (43.5 vs. 56.8 %) producer TNF-α genotypes, p = 0.296. However the low producer of IL-10 was more frequent in IBS-D vs. IBS-C vs. IBS-A/M (63.6 vs. 7.1 vs. 33,3 %) p = 0.023. Conclusions: in this group of volunteers in Mexico, the frequency of the IL-10 (-1082G/A) and TNF-α (-308G/A) genotypes was similar in IBS and controls. However, there was a greater frequency of the low producer of IL-10 in those subjects with IBS-D...

Neutrophilia induced by histamine challenge in guinea pig: The role of IL-5, IL-10 and IL-17A, but not CXCL8

Ramos-Ramírez,Patricia; Campos,María G.; Martínez-Cordero,Erasmo; García-Zepeda,Eduardo; Téllez-Jiménez,Olivia; Larios-Martínez,Karen; Bazán-Perkins,Blanca
Fonte: Instituto Nacional de Salud, Hospital Infantil de México Federico Gómez Publicador: Instituto Nacional de Salud, Hospital Infantil de México Federico Gómez
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2014 Português
Relevância na Pesquisa
66.02%
Background: Histamine is widely used as a pharmacological tool for the evaluation of airway responsiveness. Nevertheless, undesirable and contradictory effects have been described after histamine provocation tests. In previous evaluations of airway responsiveness in a guinea pig asthma model, the control groups consistently showed high neutrophil counts in bronchoalveolar lavage fluid (BALF) immediately after the histamine challenge. The changes in cytokine and chemokine levels in guinea pig lung associated with histamine induced-neutrophilia are described in this paper. Methods: Immediately and 24 h after histamine challenge, airway wall and BALF eosinophil and neutrophil counts as well as lung cytokines (IL-5, IL-10, IL-17A, TNFα and TGFβ) and chemokines (CCL11 and CXCL8) levels were evaluated. Results: Histamine inhalation generated an all-or-none bronchial response, and the dose inducing airway obstruction was similar in all guinea pigs. Immediate increases in neutrophil counts in airway wall and BALF and in IL-5, IL-10 and IL-17A levels in the lung homogenate were observed after histamine challenge. Significant correlations were found between neutrophil counts from airway wall and IL-5, IL-10 and IL-17A levels in the lung homogenate. Conclusions: Histamine inhalation induced rapid neutrophil LBA and airway wall infiltration that was not associated with CXCL8 expression but with a Th2 and Th17 cytokines that probably are involved in the recruitment and activation of neutrophils.

Increased number of IL-2, IL-2 receptor and IL-10 positive cells in premalignant lesions of the cervix

Mindiola,Raimy; Callejas,Diana; Núñez-Troconis,José; Araujo,Mary; Delgado,Mariela; Mosquera,Jesús
Fonte: Universidad del Zulia Publicador: Universidad del Zulia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2008 Português
Relevância na Pesquisa
66.08%
Previous studies have shown the involvement of the immune response in the progression of human uterine cervix cancer. The aim of this study was to determine the expression of Interleukin-2 (IL-2), IL-2 receptor (IL-2R) and Interleukin 10 (IL-10) in different grades of cervical intraepithelial neoplasias of the exocervix (CIN 1, 2 and 3), and its relationship with the serum cytokine profiles and human papilomavirus (HPV) infection status. Indirect immunofluorescence was used to study the expression of IL-2, IL-2R and IL-10 in human cervical samples from 50 patients and 9 normal controls. Serum IL-2, IL-2R and IL-10 were measured by ELISA and HPV DNA and HPV types were identified by PCR. Increased number of IL-2, IL-2R and IL-10 positive cells were observed in the cervix from patients with CIN, associated with the grades of dysplasia. A significant correlation was observed between IL-2 and IL-2R (p>0.0001), IL-2 and IL-10 (p > 0,0001), as well as IL-10 and IL-2R (p> 0.0001). Twenty percent of patients were HPV positive and 84 % of those patients were tissue cytokine positive. These results suggest that IL-2, IL-2R and IL-10 tissue expression may play a role in the development of cervical intraepithelial dysplasias.
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