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Diagnostic value of interleukin-6 and C-reactive protein on early onset bacterial infection in preterm neonates with respiratory distress

REGO, Maria Aparecida C.; MARTINEZ, Francisco Eulogio; ELIAS JR., Jorge; MUSSI-PINHATA, Marisa Marcia
Fonte: WALTER DE GRUYTER & CO Publicador: WALTER DE GRUYTER & CO
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
65.99%
Aims: To evaluate the C-reactive protein (CRP) and interleukin-6 (IL-6) as diagnostic tools for early onset infection in preterm infants with early respiratory distress (RD). Methods: CRP and IL-6 were quantified at identification of RD and 24 h after in 186 newborns. Effects of maternal hypertension, mode of delivery, Apgar score, birth weight, gestational age, mechanical ventilation, being small for gestational age (SGA), and the presence of infection were analyzed. Results: Forty-four infants were classified as infected, 42 as possibly infected, and 100 as uninfected. Serum levels of IL-6 (0 h), CRP (0 h), and CRP (24 h), but not IL-6 (24 h) were significantly higher in infected infants compared to the remaining groups. The best test for identification of infection was the combination of IL-6 (0 h) 36 pg/dL and/or CRP (24 h) 0.6 mg/dL, which yielded 93% sensitivity and 37% specificity. The presence of infection and vaginal delivery independently increased IL-6 (0 h), CRP (0 h) and CRP (24 h) levels. Being SGA also increased the CRP (24 h) levels. IL-6 (24 h) was independently increased by mechanical ventilation. Conclusions: The combination of IL-6 (0 h) and/or CRP (24 h) is helpful for excluding early onset infection in preterm infants with RD but the poor specificity limits its potential benefit as a diagnostic tool.; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)...

Comparação entre os biomarcadores inflamatórios procalcitonina (PCT), interleucina-6 (IL-6) e proteína-C reativa (PCR) para diagnóstico infeccioso e evolução de febre em pacientes neutropênicos submetidos a transplante de células tron; Comparison between inflammatory biomarkers procaltinonin (PCT), interleukin-6 (IL-6) and C-reactive protein (CRP) for infection diagnosis and fever evolution in neutropenic patients, submitted to hematopoietic stem cell transplantation (HSCT)

Massaro, Karin Schmidt Rodrigues
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 25/06/2013 Português
Relevância na Pesquisa
66.01%
Introdução: No presente estudo foram avaliados biomarcadores na ocorrência de febre em pacientes neutropênicos após transplante de células tronco hematopoiéticas (TCTH). Objetivo: O objetivo principal foi avaliar os valores séricos de biomarcadores: proteína C reativa (PCR), procalcitonina (PCT) e IL-6 (interleucina-6) que possam identificar precocemente infecção em TCTH. Outro objetivo foi fatores de risco para óbito nessa população. Métodos: Os biomarcadores foram avaliados em um estudo prospectivo que incluiu 296 pacientes neutropênicos, submetidos a TCTH autólogo ou alogênico. Os biomarcadores PCT, PCR e IL-6 foram dosados nos seguintes momentos:dia da neutropenia constatada sem febre, evento febril ou hipotermia (T < 35ºC), 24 h após a febre ou hipotermia, 72 horas após a febre ou hipotermia e febre prolongada ou seja 48 horas após a coleta no momento anterior ou na persistência da febre, cinco dias após a coleta no momento anterior. Os dados clínicos e laboratoriais, foram avaliados até a evolução para alta ou o óbito, em uma planilha Excel® 2003 e foram processados pelos programas SPSS e STATA. Os pacientes foram classificados nos seguintes grupos (I- afebril; II- febre de origem indeterminada FOI e III- febre clinica ou microbiologicamente comprovada) em relação a cada marcador estudado (PCT...

Velocidade de crescimento e níveis de interleucina-6 na artrite idiopática juvenil; Growth velocity and interleukin-6 levels in juvenile idiopathic arthritis

Souza, Letícia da Silva
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Tese de Doutorado Formato: application/pdf
Português
Relevância na Pesquisa
65.99%
Objetivos: Avaliar associações da velocidade de crescimento com marcadores inflamatórios e dose cumulativa de glicorticóide em uma coorte de pacientes com Artrite Idiopática Juvenil acompanhados por 1 ano. Material e Métodos: Foram avaliados 79 pacientes com AIJ segundo critérios da ILAR. A atividade clínica da doença foi classificada por médicos reumatologistas pediátricos. Os dados antropométricos foram mensurados e classificados de acordo com as normas da Organização Mundial da Saúde. Foram utilizadas curvas de velocidade de crescimento segundo Tanner; considerou-se baixa velocidade de crescimento valores de escore Z ≤ -2. Concentrações séricas de IL-6 foram mensuradas por ELISA no período basal, e valores acima de 1 pg/ml foram considerados elevados. Resultados: Baixa velocidade de crescimento teve uma prevalência de 25,3% e esteve associada com atividade da doença no período do seguimento (p=0,085), valores elevados de IL-6 (interleucina-6) (p=0,003), velocidade de sedimentação globular (VSG) (p=0,022) e proteína C reativa (PCR) (p=0,001) e maior dosagem cumulativa de glicocorticóide (0=0,044). Na regressão linear múltipla tendo como variável dependente a velocidade de crescimento, observou-se que somente os níveis elevados de IL-6 foram independente e negativamente associados com a velocidade de crescimento (p=0...

Níveis de interleucina-6 e fator de necrose tumoral-alfa no liquor de recém-nascidos a termo com encefalopatia hipóxico-isquêmica; Levels of interleukin-6 and tumor necrosis factor-alpha in the cerebrospinal fluid of full-term newborns with hypoxic-ischemic encephalopathy

Silveira, Rita de Cássia dos Santos; Procianoy, Renato Soibelmann
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
Relevância na Pesquisa
66.11%
Objetivo: avaliar os níveis liquóricos de IL-6 e TNF-a em recém-nascidos a termo com encefalopatia hipóxico-isquêmica (EHI), comparando-os com os de recém-nascidos controles. Metodologia: estudo caso-controle realizado no período de julho de 1999 a outubro de 2001, incluindo dois grupos de recémnascidos a termo: controle, com 20 recém-nascidos sem sepse e/ou meningite e com escore de Apgar > 9 no primeiro e quinto minutos de vida; e casos, com 15 recém-nascidos asfixiados, caracterizados pelo escore de Apgar < 4 e < 6 no primeiro e quinto minutos de vida, respectivamente, pH umbilical < 7,20 e/ou lactato arterial umbilical > 3,0 mmol/l e necessidade de ventilação com pressão positiva pelo menos durante 2 minutos após o nascimento. Foram coletadas amostras de liquor nas primeiras 48 horas de vida, para determinação dos níveis de IL-6 e TNF-a pelo método de enzimoimunoensaio. Resultados: os grupos não diferiram quanto ao peso de nascimento, idade gestacional, classificação quanto ao peso e idade gestacional, tipo de parto e tempo médio de obtenção do liquor; seus exames foram obtidos em média com 17 horas de vida. Nos recémnascidos asfixiados, as medianas dos níveis liquóricos foram: 157,5 pg/ml para IL-6 e 14...

Stress hormones increase cell proliferation and regulates interleukin-6 secretion in human oral squamous cell carcinoma cells

Bernabe, Daniel G.; Tamae, Adriano C.; Biasoli, Eder Ricardo; Oliveira, Sandra H. P.
Fonte: Academic Press Inc. Elsevier B.V. Publicador: Academic Press Inc. Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 574-583
Português
Relevância na Pesquisa
65.99%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 06/59835-0; Patients with oral cancer can have high psychological distress levels, but the effects of stress-related hormones on oral cancer cells and possible mechanisms underlying these relationships are unknown. In this study, we have investigated the effects of stress-related hormones on interleukin-6 (IL-6) secretion and proliferation of oral squamous cell carcinoma (OSCC) cells. The effects of norepinephrine (NE), and cortisol were studied in SCC9. SCC15, and SCC25 cells and effects of isoproterenol in SCC9 and SCC25 cells. Real-time PCR studies revealed constitutive beta 1- and beta 2-adrenergic receptors (beta-ARs) expression in the SCC9. SCC15, and SCC25 cells. The results showed that NE and isoproterenol significantly enhanced IL-6 mRNA expression and protein production in supernatants of SCC9 and SCC25 cells. Physiological stress levels of NE and isoproterenol (10 mu M) at 1 h elicited the most robust IL-6 increase. Regarding IL-6 secretion, 10 mu M NE induced a 5-fold increase at 1 h, 3.7-fold increase at 6 h, and 3.2-fold at 24 h in SCC9 cells. These effects were blocked by the beta-adrenergic antagonist propranolol, supporting a role for beta-ARs in IL-6 secretion. The effects of cortisol varied according to the hormone concentration. Pharmacological concentrations of cortisol (1000 nM) inhibited IL-6 production by SCC9 and SCC25 cells. Cortisol dose that simulates stress conditions (10 nM) tended to increase IL-6 expression in SCC9 cells. Hormonal doses that simulate stress conditions (10 mu M NE...

Three-year follow-up of Interleukin 6 and C-reactive protein in chronic obstructive pulmonary disease

Ferrari, Renata; Tanni, Suzana E.; Caram, Laura M.O.; Corrêa, Corina; Corrêa, Camila R.; Godoy, Irma
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
66.07%
Background: Past studies have shown that mean values of Interleukin-6 (IL-6) and C-reactive protein (CRP) do not change significantly in COPD patients over a one-year period. However, longer period follow-up studies are still lacking. Thus, the aim of this study is to evaluate plasma CRP and IL-6 concentration over three years in COPD patients and to test the association between these inflammatory mediators and disease outcome markers. Methods: A cohort of 77 outpatients with stable COPD was evaluated at baseline, and 53 (mean FEV1, 56% predicted) were included in the prospective study. We evaluated Interleukin-6 (IL-6), C-reactive protein (CRP), six-minute walking distance (6MWD), and body mass index (BMI) at baseline and after three years. Plasma concentration of IL-6 was measured by high sensitivity ELISA, and CRP was obtained by high sensitivity particle-enhanced immunonephelometry. Results: IL-6 increased significantly after 3 years compared to baseline measurements [0.8 (0.5-1.3) vs 2.4 (1.3-4.4) pg/ml; p < 0.001] and was associated with worse 6MWD performance. In the Cox regression, increased IL-6 at baseline was associated with mortality [Hazard Ratio (95% CI) = 2.68 (0.13, 1.84); p = 0.02]. CRP mean values did not change [5 (1.6-7.9) vs 4.7 (1.7-10) pg/L; p = 0.84]...

Evaluation of effluent markers cancer antigen 125, vascular endothelial growth factor, and interleukin‐6: relationship with peritoneal transport

Rodrigues, A.; Martins, M.; Santos, M.; Fonseca, I.; Oliveira, J.; Cabrita, A.; Castro e Melo, J.; Krediet, R.
Fonte: Peritoneal Dialysis Bulletin, Inc. Publicador: Peritoneal Dialysis Bulletin, Inc.
Tipo: Artigo de Revista Científica
Publicado em //2004 Português
Relevância na Pesquisa
65.99%
O pdf deste artigo (p. 8-12) contém mais 2 artigos relacionados:(p. 2-7) e (p. 13-17); Peritoneal hyperpermeability has been associated with increased levels of effluent vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6). Mesothelial cells can produce various vasoactive substances besides VEGF. A large mesothelial mass may possibly lead to high dialysate VEGF concentrations and may partly explain some cases of peritoneal hyperpermeability during a patient’s early months on peritoneal dialysis (PD). Early peritoneal fast transport may therefore not necessarily be associated with systemic inflammation. To investigate the relationship of effluent markers and peritoneal transport, we measured the appearance rates of cancer antigen 125 (CA125), VEGF, and IL-6 in 4-hour effluents from 69 peritoneal equilibration tests (PETs) using 3.86% glucose solution. At the same time, we measured serum VEGF and IL-6. Our analyses included an early group (EG), whose members had been on PD for 4.6 ± 3.3 months, and a later group (LG), whose members had been on PD for 30 ± 17 months. In EG, dialysate-to-plasma creatinine at 4 hours (D/PCr240) correlated significantly with effluent CA125/min (r = 0.51, p = 0.006) and VEGF/min (r = 0.57...

Tumor necrosis factor-alpha, interleukin-1beta and interleukin-6 in the cerebrospinal fluid of newborn with meningitis

Krebs,Vera Lúcia Jornada; Okay,Thelma Suely; Okay,Yassuhiko; Vaz,Flávio Adolfo Costa
Fonte: Academia Brasileira de Neurologia - ABNEURO Publicador: Academia Brasileira de Neurologia - ABNEURO
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2005 Português
Relevância na Pesquisa
66.07%
OBJECTIVE: To analyze the usefulness of determining the cerebrospinal fluid(CSF) levels of tumor necrosis factor-alpha(TNF-alpha),interleukin-1beta(IL-1beta) and interleukin-6(IL-6)for the early diagnosis and evaluation of the prognosis of neonatal meningitis. METHOD: We studied 54 newborn that underwent lumbar puncture.Thirty patients had meningitis and 24 were the control group.CSF and sera were obtained at the moment of suspicion of meningitis and stored at -70(0)C.Cytokines were performed by enzyme-linked immunosorbent assay method. RESULTS: CSF cytokines were detected in all the newborn with meningitis.TNF-alpha was detected in the CSF in 63.3% of the neonates, IL-1beta in 73.3% and IL-6 in 96.6%.The CSF levels were significantly higher than serum in neonates with meningitis.There was no correlation between the CSF levels of cytokines and neurologic complications. CONCLUSION: The detection of TNF-alpha, IL-1beta and IL-6 in the CSF is of great value in order to achieve a early diagnosis of neonatal meningitis.Among the three cytokines analyzed, IL-6 was the best indicator of meningeal inflammation.

Effect of intraoperative intravenous lidocaine on pain and plasma interleukin-6 in patients undergoing hysterectomy

Oliveira,Caio Marcio Barros de; Sakata,Rioko Kimiko; Slullitel,Alexandre; Salomão,Reinaldo; Lanchote,Vera Lucia; Issy,Adriana Machado
Fonte: Sociedade Brasileira de Anestesiologia Publicador: Sociedade Brasileira de Anestesiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2015 Português
Relevância na Pesquisa
66.06%
BACKGROUND AND OBJECTIVES: Interleukin-6 is a predictor of trauma severity. The purpose of this study was to evaluate the effect of intravenous lidocaine on pain severity and plasma interleukin-6 after hysterectomy. METHOD: A prospective, randomized, comparative, double-blind study with 40 patients, aged 18-60 years. G1 received lidocaine (2 mg kg-1 h-1) or G2 received 0.9% saline solution during the operation. Anesthesia was induced with O2/isoflurane. Pain severity (T0: awake and 6, 12, 18 and 24 h), first analgesic request, and dose of morphine in 24 h were evaluated. Interleukin-6 was measured before starting surgery (T0), 5 h after the start (T5), and 24 h after the end of surgery (T24). RESULTS: There was no difference in pain severity between groups. There was a decrease in pain severity between T0 and other measurement times in G1. Time to first supplementation was greater in G2 (76.0 ± 104.4 min) than in G1 (26.7 ± 23.3 min). There was no difference in supplemental dose of morphine between G1 (23.5 ± 12.6 mg) and G2 (18.7 ± 11.3 mg). There were increased concentrations of IL-6 in both groups from T0 to T5 and T24. There was no difference in IL-6 dosage between groups. Lidocaine concentration was 856.5 ± 364.1 ng mL-1 in T5 and 30.1 ± 14.2 ng mL-1 in T24. CONCLUSION: Intravenous lidocaine (2 mg kg-1 h-1) did not reduce pain severity and plasma levels of IL-6 in patients undergoing abdominal hysterectomy.

Muscle strength but not functional capacity is associated with plasma interleukin-6 levels of community-dwelling elderly women

Oliveira,D.M.G.; Narciso,F.M.S.; Santos,M.L.A.S.; Pereira,D.S.; Coelho,F.M.; Dias,J.M.D.; Pereira,L.S.M.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2008 Português
Relevância na Pesquisa
65.99%
The association of plasma interleukin-6 (IL-6) levels, muscle strength and functional capacity was investigated in a cross-sectional study of community-dwelling elderly women from Belo Horizonte, Brazil. Elderly people who present controlled chronic diseases with no negative impact on physical, psychosocial and mental functionality are considered to be community-dwelling. Psychological and social stress due to unsuccessfully aging can represent a risk for immune system disfunctions. IL-6 levels, isokinetic muscle strength of knee flexion/extension, and functional tests to determine time required to rise from a chair and gait velocity were measured in 57 participants (71.21 ± 7.38 years). Serum levels of IL-6 were measured in duplicate and were performed within one single assay (mouse monoclonal antibody against IL-6; High-Sensitivity, Quantikine®, R & D Systems, USA; intra-assay coefficient of variance = 6.9-7.4%; interassay coefficient of variance = 9.6-6.5%; sensitivity = 0.016-0.110 pg/mL; mean = 0.039 pg/mL). Muscle strength was assessed with the isokinetic dynamometer Biodex System 3 Pro®. After the Shapiro-Wilk normality test was applied, correlations were investigated using Spearman and Kruskal-Wallis tests. Post hoc analysis was performed using the Dunn test. A significant negative correlation was observed between plasma IL-6 levels (1.95 ± 1.77 pg/mL) and muscle strength for knee flexion (70.70 ± 21.14%; r = -0.265; P = 0.047) and extension (271.84 ± 67.85%; r = -0.315; P = 0.017). No significant correlation was observed between IL-6 levels and the functional tests (time to rise from a chair = 14.65 ± 2.82 s and gait velocity = 0.95 ± 0.14 m/s). These results suggest that IL-6 is associated with reduced muscle strength.

Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells

Yang,R.; Lin,Q.; Gao,H.B.; Zhang,P.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2014 Português
Relevância na Pesquisa
65.99%
In the current literature, there is evidence that psychological factors can affect the incidence and progression of some cancers. Interleukin 6 (IL-6) is known to be elevated in individuals experiencing chronic stress and is also involved in oncogenesis and cancer progression. However, the precise mechanism of IL-6 induction by the stress-related hormone norepinephrine (NE) is not clear, and, furthermore, there are no reports about the effect of NE on IL-6 expression in gastric epithelial cells. In this study, we examined the effect of NE on IL-6 expression in immortalized human gastric epithelial cells (GES-1 cells). Using real-time PCR and enzyme-linked immunoassay, we demonstrated that NE can induce IL-6 mRNA and protein expression in GES-1 cells. The induction is through the β-adrenergic receptor-cAMP-protein kinase A pathway and mainly at the transcriptional level. Progressive 5′-deletions and site-directed mutagenesis of the parental construct show that, although activating-protein-1 (AP-1), cAMP-responsive element binding protein (CREB), CCAAT-enhancer binding protein-β (C/EBP-β), and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) binding sites are all required in the basal transcription of IL-6...

Staphylococcus aureus regulates secretion of interleukin-6 and monocyte chemoattractant protein-1 through activation of nuclear factor kappaB signaling pathway in human osteoblasts

Ning,Rende; Zhang,Xianlong; Guo,Xiaokui; Li,Qingtian
Fonte: Brazilian Society of Infectious Diseases Publicador: Brazilian Society of Infectious Diseases
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2011 Português
Relevância na Pesquisa
66.1%
OBJECTIVE: Activation of nuclear factor kappaB by diverse bacteria regulates the secretion of chemokines and cytokines. Staphylococcus aureus (S. aureus)-infected osteoblasts can significantly increase the secretion of interleukin-6 and monocyte chemoattractant protein-1. The aim of this study was to investigate whether S. aureus can activate nuclear factor kappaB in human osteoblasts, and whether the activation of nuclear factor kappaB by S. aureus regulates the secretion of interleukin-6 and monocyte chemoattractant protein-1. METHODS: Immunoblot and electrophoretic mobility shift assay were used to detect the degradation of IκBa and activation of nuclear factor kappaB in human osteoblasts in response to S. aureus, respectively. Enzyme-linked immunosorbent assay was used to measure the secretion of interleukin-6 and monocyte chemoattractant protein-1 in the supernatants. Lastly, carbobenzoxyl-l-leucinyl-l-leucinyl-l-leucinal, an inhibitor of the nuclear factor kappaB, was used to determine if activation of nuclear factor kappaB by S. aureus in human osteoblasts regulates the secretions of interleukin-6 and monocyte chemoattractant protein-1. RESULTS: Our results for the first time demonstrated that S. aureus can induce the degradation of IκBa and activation of nuclear factor kappaB in human osteoblasts in a time and dose-dependent manner. In addition...

Serum levels of interleukin-6 in contacts of active pulmonary tuberculosis

Lopes,Fernando Henrique Azevedo; Assis,Lívia Coelho de; Pires Neto,Roberto da Justa; Botelho,Karine Paschoal; Sá,Kélvia Miranda; Frota,Cristiane Cunha; Correia,José Walter; Freitas,Max Victor Carioca
Fonte: Sociedade Brasileira de Patologia Clínica; Sociedade Brasileira de Patologia; Sociedade Brasileira de Citopatologia Publicador: Sociedade Brasileira de Patologia Clínica; Sociedade Brasileira de Patologia; Sociedade Brasileira de Citopatologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2013 Português
Relevância na Pesquisa
66.05%
INTRODUCTION: It is estimated that over two billion individuals are infected by Mycobacterium tuberculosis worldwide. Interleukin-6 (IL-6) is an important cytokine whose serum levels are commonly high in active pulmonary tuberculosis (TB). IL-6 screening in contacts of patients with TB may be useful to monitor the progress of the infectious process and to infer the risk of progression to active disease. OBJECTIVE: To evaluate the serum levels of interleukin-6 in contacts of patients with active pulmonary tuberculosis and to compare them with two other groups: a) patients affected by active pulmonary tuberculosis; b) non-contacts of tuberculosis. METHODS: Cross-sectional study with 15 contacts of patients with active pulmonary tuberculosis, selected according to the protocol recommended by the Ministry of Health. The serum levels of interleukin-6 were measured by Enzyme-linked immunosorbent assay (ELISA). The same test was also applied in the two comparison groups: 38 patients with active pulmonary tuberculosis (confirmed by clinical examination, X-rays of the chest and baciloscopy) and 63 non-contacts (healthy blood donors). RESULTS: In the contact group, the median IL-6 concentration was 1.7 pg/ml (0.96-4.8 pg/ml). For those affected by active pulmonary tuberculosis and non-contact individuals...

Enhanced expression of osteocalcin mRNA in human osteoarthritic trabecular bone of the proximal femur is associated with decreased expression of interleukin-6 and interleukin-11 mRNA

Findlay, D.; Atkins, G.; Forwood, M.; Kuliwaba, J.; Fazzalari, N.
Fonte: Amer Soc Bone & Mineral Res Publicador: Amer Soc Bone & Mineral Res
Tipo: Artigo de Revista Científica
Publicado em //2000 Português
Relevância na Pesquisa
66.06%
Few studies have investigated the factors or mechanisms that may lead to structural changes in OA bone. This study examines the in vivo expression of messenger RNA encoding the osteoclastogenic cytokines interleukin-6 (IL-6) and interleukin-11 (IL-11), together with the osteoblastic marker osteocalcin (OCN) and the calcitonin receptor (CTR), which in bone is exclusively expressed by osteoclasts. Total RNA was isolated from intertrochanteric trabecular bone from OA patients, and from controls taken at autopsy. The patterns of mRNA expression of IL-6, IL-11, OCN, and CTR were examined using reverse-transcription polymerase chain reaction (RT-PCR) by determining the relative ratios of the amplified products with respect to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Both IL-6 and IL-11 mRNA were significantly less abundant in OA than in the control group. Expression of IL-11 mRNA decreased significantly with age for both groups. OCN mRNA expression was significantly more abundant in OA, and there was no significant difference for CTR mRNA between the two groups. For both OCN and CTR in OA, expression increased significantly with increasing age. These differences in expression between the OA and control groups are consistent with an hypothesis that biochemical and genetic factors in bone can contribute or perhaps underlie the degenerative joint changes seen in OA.

A randomized trial of supplementation with docosahexaenoic acid-rich tuna oil and its effects on the human milk cytokines interleukin 1beta, interleukin 6, and tumor necrosis factor alpha

Hawkes, J.; Bryan, D.L.; Makrides, M.; Neumann, M.; Gibson, R.
Fonte: Amer Soc Clinical Nutrition Publicador: Amer Soc Clinical Nutrition
Tipo: Artigo de Revista Científica
Publicado em //2002 Português
Relevância na Pesquisa
66.05%
Background: Increased consumption of n-3 long-chain polyunsaturated fatty acids (PUFAs) has been recommended during pregnancy and lactation. The production of proinflammatory cytokines by human peripheral blood mononuclear cells (PBMCs) can be modified by dietary n-3 PUFAs. Objective: We sought to determine whether dietary supplementation of lactating women with docosahexaenoic acid (DHA) can modulate the concentration of cytokines in the aqueous phase of human milk and the production of cytokines by human milk cells (HMCs) and PBMCs. Design: In this double-blind, prospective, randomized trial, mothers of healthy full-term infants were asked to consume daily a nutritional supplement of 2000 mg oil containing either placebo (n = 40), 300 mg DHA + 70 mg eicosapentaenoic acid (EPA; n = 40), or 600 mg DHA + 140 mg EPA (n = 40). The fatty acid composition of plasma, PBMCs, milk, and HMCs from lactating mothers at 4 wk postpartum was measured by gas chromatography. The concentration of interleukin 6 and tumor necrosis factor in milk and the production of interleukin 1ß, tumor necrosis factor , and interleukin 6 by PBMCs and HMCs after stimulation with lipopolysaccharide was measured by enzyme-linked immunosorbent assay. Results: At 4 wk postpartum...

Early expression and cellular localization of proinflammatory cytokines interleukin-1[beta], interleukin-6, and tumor necrosis factor-[alpha] in human traumatic spinal cord injury

Yang, L.; Blumbergs, P.; Jones, N.; Manavis, J.; Sarvestani, G.; Ghabriel, M.
Fonte: Lippincott Williams & Wilkins Publicador: Lippincott Williams & Wilkins
Tipo: Artigo de Revista Científica
Publicado em //2004 Português
Relevância na Pesquisa
66.12%
Study Design. Post-traumatic inflammatory response was studied in 11 human cases of acute spinal cord contusion injury. Objectives. To examine the inflammatory cellular response and the immunocytochemical expression and localization of interleukin-1[beta], internleukin-6, and tumor necrosis factor-[alpha]in human spinal cord after contusion injury. Summary of Background Data. The post-traumatic inflammatory response plays an important role in secondary injury mechanisms after spinal cord injury, and inter-leukin-1[beta], internleukin-6, and tumor necrosis factor-[alpha] are key inflammatory mediators. Methods. The study group comprised 11 patients with spinal cord contusion injury and 2 normal individuals. Histologic and immunocytochemical assessments were undertaken to evaluate the inflammatory cellular response and the immunoexpression of interleukin-1[beta], internleukin-6, and tumor necrosis factor-[alpha] in the injured human spinal cord. The cellular sources of interleukin-1[beta], internleukin-6, and tumor necrosis factor-[alpha] were elucidated by immunofluorescence double-labeled confocal imaging. Results. Increased immunoreactivity of interleukin-1[beta], internleukin-6, and tumor necrosis factor-[alpha]was detected in neurons 0.5 hour after injury...

Uterine epithelial cells synthesize granulocyte-macrophage colony-stimulating factor and interleukin-6 in pregnant and nonpregnant mice

Robertson, S.A.; Mayrhofer, G.; Seamark, R.F.
Fonte: Society for the Study of Reproduction Publicador: Society for the Study of Reproduction
Tipo: Artigo de Revista Científica
Publicado em //1992 Português
Relevância na Pesquisa
66.08%
Cytokine secretion by endometrial cells from estrous and mated mice was measured using specific bioassays. The granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) contents of uterine intraluminal fluid were elevated greater than 20-fold and 250-fold respectively following mating, and both cytokines were synthesized in abundance in vitro by uterine cells harvested at estrus and on Day 1 of pregnancy. Synthesis was not impaired in genetically lymphocyte-deficient nude, SCID, or beige mice. To determine the cellular origin of the cytokines, a panning technique employing monoclonal antibodies against a range of leukocyte and other lineage markers was used to isolate uterine cell subsets in vitro. These experiments identified glandular and/or luminal epithelial cells as the major source of GM-CSF and IL-6 in estrous and pregnant uteri. Stromal fibroblasts also synthesized IL-6, as did macrophages in mated mice. Epithelial cells harvested from midgestation uteri secreted GM-CSF and IL-6 in quantities similar to those of cells from estrous and mated mice. Bioactivities of both cytokines derived from epithelial cells were neutralized by specific antibodies, and size-exclusion chromatography of conditioned media from uterine cells revealed peaks of GM-CSF and IL-6 bioactivity with M(r) 23...

Molecular mechanisms of the metabolic adaptation to physical exercise: Acute response of the liver and the role of interleukin-6; Molekulare Mechanismen der metabolischen Anpassung an körperliche Aktivität: Akute Antwort der Leber und die Rolle von Interleukin-6

Hoene, Miriam
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
66.1%
Understanding the molecular mechanisms that determine the beneficial metabolic response to physical exercise may open up new therapeutic options to prevent and improve Type 2 Diabetes and related metabolic disorders. The focus of this thesis was on the liver as the main regulator of energy homeostasis and on interleukin-6 (IL-6) because it is released from the working muscle and could signal directly to the liver. A single bout of non-exhaustive endurance exercise caused a rapid and strong transcriptional response of several key regulators of glucose and fatty acid metabolism in the liver of mice that was more pronounced than in the working muscle at this early time point. The amount of insulin receptor substrate-2 protein was increased and insulin signalling, induced by glucose-stimulated endogenous insulin secretion, was amplified. Different stress-responsive pathways were transiently activated in the liver, most notably, mitogen-activated protein kinase (MAPK) signalling, as evidenced by the increased phosphorylation of c-Jun terminal kinase and an extracellular signal related kinase isoform and induction of MAPK target genes. IL-6-type cytokine signalling and p53 were also activated. Exercise-induced oxidative stress was not the stimulus for the hepatic stress response...

Serielle Lactatbestimmung als prognostischer Parameter nach Herzoperationen im Kindesalter - Vergleich mit der prognostischen Aussagekraft von Procalcitonin, Interleukin 6 und 8 in Bezug auf das postoperative Geschehen nach Herzoperationen im Kindesalter; Serial measurement of lactate as a prognostic parameter after heart surgery in children- a comparison with the prognostic meaning of procalcitonin, interleukin 6 and 8 referring to the postoperative course in infancy

Weiß, Christine
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
66.18%
Lactat entsteht bei der anaeroben Glykolyse. Das postoperative Lactat ermöglicht, das Ausmaß der intraoperativen Gewebehypoxie mit eventuellen Folgeschäden abzuschätzen. Procalcitonin, ein aus 116 Aminosäuren bestehendes Protein, und die Interleukine 6 und 8, zur Gruppe der Zytokine gehörend, dienen als Inflammationsmarker. Die vorliegende Arbeit besteht aus zwei Teilen. Im ersten Teil wurde im Rahmen einer retrospektiven Studie bei 253 an der Universitäts-Kinderklinik Tübingen in der Zeit von März 1997 bis Mai 1998 am Herzen operierten Kinder die postoperativen Lactatspiegel bestimmt. Die Kinder waren zwischen einem Tag und 16,5 Jahren alt. Ein Ziel war es herauszufinden, ob das Lactat ein Parameter ist, der zur Vorhersage des postoperativen Outcomes beitragen kann. Im zweiten Teil, einer prospektiven Studie, wurden Lactatspiegel bei 51 Kindern, die in der Zeit von August 2000 bis Februar 2001 ebenfalls an der herzchirurgischen Abteilung der Universitätskinderklinik Tübingen am Herzen operiert wurden, untersucht. Diese Kinder waren zwischen 4 Tagen und 16 Jahre alt. Die Lactatverläufe wurden bis zum 6. postoperativen Tag analysiert. Zusätzlich wurde sieben Mal bis zum 6. postoperativen Tag die Bestimmung von Procalcitonin (PCT)...

Role of interleukin-6, gamma interferon and adenosine deaminase markers in management of pleural effusion patients

Marie,MAM; John,J; Krishnappa,L Gowda; Gopalkrishnan,S; Bindurani,SR; CS,P
Fonte: West Indian Medical Journal Publicador: West Indian Medical Journal
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2013 Português
Relevância na Pesquisa
66%
OBJECTIVE: Pleural effusion is a common diagnostic and clinical problem. Neoplasms and tuberculosis are the most frequent diagnostic causes of such effusions. Conventional laboratory methods for diagnosis of such effusion are inefficient because tubercle bacilli are rarely seen in direct examinations of pleural fluid. The present study evaluates interleukin-6 (IL-6), gamma interferon (IFN-γ) and adenosine deaminase (ADA) as diagnostic tools in pleural effusion. METHODS: Interleukin-6, IFN-γ and ADA were measured in pleural fluid from the patients, with exudative pleural effusion from tuberculous, malignant and postpneumonic origin and transudative pleural effusion ofsystemic origin in order to evaluate the diagnostic utility ofthese. RESULTS: The three markers were detectable in all effusions with significantly high levels in exudative as compared to transudative effusions. There was a statically significant difference noticed in tuberculous as compared to malignant andpostpneumonic origin and transudative pleural effusion. CONCLUSION: We concluded that IL-6, IFN-γ and ADA levels in pleural effusion are sensitive parameters to differentiate an exudate from a transudate and they can also differentiate exudates of different aetiology. Finally...