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Microbiological characterization of table olives commercialized in Portugal in respect to safety aspects

Pereira, Ana Paula; Pereira, J.A.; Bento, Albino; Estevinho, Leticia M.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
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Table olives are a traditional component of the Mediterranean diet and are largely consumed in the world. There are different trade treatments that originate different types of olives. The aim of the present work was to proceed to the microbiological characterization of table olives commercialized in the Portuguese market, with respect to their microbiological safety. The microbiological characterization was made in the olive pulp and packing brine of thirty-five table olives samples of different types and trade treatments, namely natural olives Cv. Galega (NOG), natural turning colour olives (NTCO), green olives (GO), black ripe olives (BO) and natural olives purchased in the traditional market and obtained from traditional producers (NOt). Simultaneously it was verified specific legislation of the table olives in what it concerns to labeling rules, pH values and the identification of isolated yeasts. In general, table olives consumed showed acceptable security with exception of four NOt samples that presented Staphylococcus aureus. In a considerable number of samples high number of microorganisms indicators of contaminations were observed that reveals the need of optimization the hygienic procedures during production process to improve the quality and safety of table olives. During the work seven yeasts were isolated from olive pulp and brine.

Portuguese bee pollen: palynological study, nutritional and microbiological evaluation

Estevinho, Leticia M.; Rodrigues, Sandra; Pereira, Ana Paula; Feás, Xesús
Fonte: Wiley Publicador: Wiley
Tipo: Artigo de Revista Científica
Português
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Bee pollen is one of the richest and purest natural foods, used in the folk medicine, clinical practices, and food and pharmaceutical industries. This work aims to characterise, for the first time in Portugal, the palynological origin, nutritional value and microbiological safety of this beehive product. It identified ten botanical families, and the more frequent were Boraginaceae and Ericaceae. We have evaluated the water content, aw, pH, reducing sugars, ash, carbohydrate, proteins, lipids, fatty acids and energy. The microorganisms studied were the aerobic mesophiles, moulds and yeasts, faecal coliforms, Escherichia coli, Staphylococcus aureus, Salmonella and sulphite-reducing clostridia. The studied pollens are nutritionally well balanced, and contain high levels of moisture, proteins, fat, energy, ash, carbohydrates, reducing sugars, essential n-3 fatty acids and good ratios of polyunsaturated fatty acids (PUFA)/saturated fatty acids. In fact, the PUFA represent 66% of the total fatty acids. The samples had good microbiological quality, as none of the samples had toxigenic species.

Palynological, physicochemical, and microbiological attributes of organic lavender (Lava ndula stoechas) honey from Portugal

Estevinho, Leticia M.; Vázquez-Tato, Maria Pilar; Seijas, Julio; Feás, Xesús
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Artigo de Revista Científica
Português
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At the present time, the quality, integrity, sanitation, and nutritional value of honeys receive attention on an international level due to the increasing content of chemicals in the aforementioned matrix. The present study aims to characterize organic honey (n=73) from Northeast Portugal, with respect to floral nectar origin, physicochemical parameters, microbial safety, and commercial quality. All organic honey samples can be classified as monofloral lavender (Lavandula stoechas L.), exceed in quality the international physicochemical standards, and show low microbiological counts (yeast, moulds, and aerobic mesophiles), with negative results in respect to faecal coliforms, Salmonella, and sulphite-reducing clostridia. Correlating the palynological, physicochemical, and microbiological results is necessary in order to check the authenticity, quality, and sanitation of honey.

Processo de secagem da glândula mamária de bovinos da raça Holandesa: avaliação das características microbiológicas da secreção láctea durante o período seco; The involution of the mammary gland in Holstein cows: evaluation of the microbiological characteristics of the lacteal secretion in the dry period.

BIRGEL, Daniela Becker; BIRGEL JÚNIOR, Eduardo Harry; POGLIANI, Fabio Celidonio; RAIMONDO, Raquel Fraga e Silva; BIRGEL, Eduardo Harry; ARAÚJO, Wanderley Pereira de
Fonte: São Paulo Publicador: São Paulo
Tipo: Artigo de Revista Científica
Português
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Com o objetivo de avaliar as características microbiológicas da secreção láctea durante o processo de secagem da glândula mamária de vacas da raça Holandesa, foram colhidas amostras de 44 mamas na última ordenha antes do início do processo de secagem, durante a evolução do processo de secagem (1°,3°, 5°, ~, 10°, 15°, 30° e 45° dia do período seco) e no retorno à lactação. Após os primeiros jatos de secreção láctea terem sido desprezados, procedeu-se a sua colheita asséptica, para ser utilizada no exame microbiológico, No retorno da lactação observou-se que a frequência de isolamento bacteriano (46,15%) foi significantemente menor do que o encontrado durante o período seco. Entre a última ordenha antes do início do processo de secagem e o 45°dia do período seco não houve diferenças estatísticas entre as frequências de isolamentos bacterianos que oscilaram entre 72,73 % e 84,09 %. Os principais gêneros bacterianos isolados foram Staphy/ococcus spp., Conjnebacterium spp. e Sirepiococcus spp. A análise da dinâmica da população bacteriana durante o período seco permitiu afirmar que a taxa de cura das infecções existentes foi igual a 40,63 %, enquanto as taxa de novas infecções e ré-infecções durante o período seco foram...

Comparison of three experimental designs employed in gentamicin microbiological assay through agar diffusion

LOURENÇO, Felipe Rebello; PINTO, Terezinha de Jesus Andreoli
Fonte: Universidade de São Paulo, Faculdade de Ciências Farmacêuticas Publicador: Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Tipo: Artigo de Revista Científica
Português
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Gentamicin is a broad-spectrum antibiotic complex produced by actinomycetes belonging to Micromonospora genus and classified among aminoglycoside antibiotics, used in the treatment of serious infections derived from Gram-negative microorganisms. Alterations of their antimicrobial activity not shown in chemical assays can be evaluated through microbiological assays. The aim of this work was to compare 5 x 1, 2 x 2 and 3 x 1 experimental designs, evaluating validation parameters of specificity, linearity, range, precision, and accuracy for each experimental design in different levels of concentration, presentation, and lots. It consisted of 81 assays (in 3 replicas) of gentamicin microbiological dosage. The concentrations of the solutions used were employed in a range from 1.0 μg/mL to 5.0 μg/mL, diluted in phosphate buffer 0.1 M pH 8.0. Antibiotic medium number 11 was used, with Staphyloccocus epidermis (ATCC 12228). 21ml of medium were used as base layer and 4 ml of medium inoculated at 1% were used as surface layer. The dishes were incubated for 18 hours at 37 ± 1 ºC. The three designs employed showed adequate specificity for analysis of dermatological cream and injectable solution containing gentamicin sulphate. They also showed accuracy and linearity in the range evaluated...

Microbiological and histopathological aspects of canine pyometra; Aspectos microbiológicos e histopatológicos da piometria canina

COGGAN, Jennifer Anne; MELVILLE, Priscilla Anne; OLIVEIRA, Clair Motos de; FAUSTINO, Marcelo; MORENO, Andréa Micke; BENITES, Nilson Roberti
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica
Português
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As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health. The microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the E. coli strains were identified using PCR method. This study also consisted of the counting of microorganisms colonies forming units in samples of intrauterine content, tests of antimicrobial susceptibility of the E. coli isolates and the histological examination of the uterus. E. coli was the most prevalent microorganism isolated (76.6%) and 120 strains (79.5%) were positive for sfa, 86 (56.9%) were positive for cnf, 87 (57.6%) were positive for pap, 52 (34.4%) were positive for hly, 51 (33.8%) were positive for iuc and 5 (3.3%) were positive for afa genes. One observed more sensitivity of E. coli to norfloxacin, polimixin B, sulphazotrin, chloranfenicol and enrofloxacin. In 42% of the samples of uterine walls where microorganisms were isolated, the sizes of the areas of the inflammatory responses corresponded to 39-56%. Virulence factors were identified in 98.0% of the strains evaluated...

Doseamento microbiológico de gentamicina por difusão em agar - proposta de delineamento experimental; Microbiological assay of gentamicin sulfate by agar diffusion - proposal of experimental design

Lourenço, Felipe Rebello
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 18/12/2006 Português
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A gentamicina é um complexo antibiótico de largo espectro, produzido por actinomicetos do gênero Micromonospora e classificado entre os antibióticos aminoglicosídeos, utilizado no tratamento de infecções graves, devidas a microrganismos Gram-negativos. Alterações da sua atividade antimicrobiana, não demonstradas pelos ensaios químicos, podem ser avaliadas pelos ensaios microbiológicos. O objetivo deste trabalho foi comparar os delineamentos experimentais 5 x 1, 2 x 2 e 3 x 1, avaliando-se os parâmetros de validação de especificidade, linearidade, faixa ou intervalo, precisão e exatidão para cada delineamento experimental em diferentes níveis de concentração, apresentações e lotes. O plano de trabalho constituiu-se na realização de 81 ensaios (em 3 réplicas) de doseamento microbiológico de gentamicina. As concentrações das soluções empregadas foram preparadas numa faixa de 1,0 µg/mL a 5,0 µg/mL, diluídos em tampão fosfato 0,1 M pH 8,0. O meio utilizado foi o meio antibiótico no. 11, com Staphyloccocus epidermidis (ATCC 12228). Empregou-se 21 mL de meio como camada base e 4 mL de meio inoculado à 1% como camada superfície. As placas foram incubadas por 16-18 horas à 37 ± 1 °C. Os três delineamentos empregados apresentaram especificidade adequada para análise de creme dermatológico e solução injetável contendo sulfato de gentamicina. Também apresentaram exatidão e linearidade no intervalo avaliado. Os delineamentos não apresentaram diferença significativa quanto a precisão. Os resultados foram comparados através da determinação de índices de capacidade do sistema de medição. A análise estatística demonstrou que não há diferença significativa entre os resultados obtidos pelos delineamentos 5 x 1...

Aspectos da qualidade da tetraciclina em preparações farmacêuticas sólidas. Correlação entre os métodos de dosagem por cromatografia líquida de alta eficiência e turbimético; Aspects of the quality of tetracycline in solid pharrnaceutical forrnulations. Correlation between HPLC and microbiological methods

Yamamoto, Célia Hitomi
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 26/03/1999 Português
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As tetraciclinas são encontradas no mercado sob várias formas farmacêuticas, sendo provenientes de diversos laboratórios farmacêuticos. Com o objetivo de avaliar a qualidade destes medicamentos, foram realizados os ensaios de dissolução in vitro e determinação quantitativa. Um total de 38 amostras de cápsulas de cloridrato de tetraciclina, fosfato de tetraciclina e cloridrato de oxitetraciclina e drágea de cloridrato de doxiciclina, englobando 12 fabricantes, foram analisadas. A dissolução do princípio ativo foi determinada para todas as amostras, conforme o método recomendado pela USP XXIII. Das amostras, duas foram reprovadas e outras quatro foram aprovadas após o reteste. A variação dos valores individuais obtidos no ensaio de dissolução para cada amostra, foi significativa, apresentando coeficiente de variação de até 14,2 %. A determinação quantitativa através do método microbiológico turbidimétrico empregando Staphylococcus aureus ATCC 29737, resultou em duas amostras de um mesmo fabricante com potência muito abaixo do limite especificado de 90 a 125 % do valor rotulado, com 55.5 e 68,7 %. Estudo comparativo desta metodologia com o método de cromatografia líquida de alta eficiência (CLAE) foi realizado. Para isto...

Mineral water: A microbiological approach

Falcone-Dias, Maria Fernanda; Emerick, Guilherme L.; Farache-Filho, Adalberto
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 556-562
Português
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The microbiological quality of bottled mineral water of various domestic brands sold in Brazil was investigated, with particular focus on the heterotrophic plate count (HPC). Neither total coliforms nor Escherichia coli were found in any 1.5 L bottle samples. Total coliforms were found in 2.9% of the small bottles, while in 20 L bottles the presence of total coliforms and E. coli was demonstrated in 15.5 and 2.4% of samples, respectively. Pseudomonas aeruginosa was detected in 4.3, 4.5 and 9.5% of small, 1.5 and 20 L bottles, respectively. In 36.4% of the samples of 1.5 L bottles, the HPC was above 500 cfu/mL. This percentage of samples with an HPC above 500 cfu/mL increased to 52.0 and 61.9% in small and 20 L bottles, respectively. Higher contamination by total coliforms, E. coli, P. aeruginosa and HPCs occurred in 20 L bottles. In conclusion, several samples in this study were outside the international quality standard for mineral water and the large number of samples with high HPCs shows that more work must be done on the use of HPC in mineral water and the damaging effects that these microorganisms may cause to humans. The bottled mineral water was confirmed as a particularly important public health problem, due to the poor microbiological quality of the products that are marketed. © IWA Publishing 2012.

Microbiological control of moisturizing mask formulation added of hibiscus flowers, assai palm, black mulberry and papaw glycolic extracts

Oliveira, Marcela B.; Vieira, Daniela C. M.; Fiúza, Thalita M.; Salgado, Hérida R. N.; Chorilli, Marlus
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 342-345
Português
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The microbiological control of moisturizing mask formulation added of hibiscus flowers, assai palm, black mulberry and papaw glycolic extracts, determining the number of viable microorganisms and possible presence of pathogenic. The moisturizing mask formulation was composed of zinc oxide (5. 0%) and moisturizing cream constituted of triceteareth-4 phosphate (and) cetyl alcohol (and) stearyl alcohol (and) sodium cetearyl sulfate (and) oleth-10 (qs 50g). To this formulation was added hibiscus flowers glycolic extract (2. 5%), assai palm glycolic extract (1. 5%), black mulberry glycolic extract (1. 5%) and papaw glycolic extract (2. 0%). The formulation was stored in aseptically clean recipients, away from humidity and light, in fresh and airy places. The results of the microbiological analysis on the counting of aerobic mesophilic microorganisms (bacteria and fungi), of the above mentioned formulation, revealed a bioburden < 10 CFU/mL in all samples. Such data indicate adequate microbiological quality of the tested products, according to official recommendations. Furthermore, it was not detected the presence of pathogenic microorganisms, assuring the harmlessness of the formulation. The results lead us to conclude that the formulation and raw materials analyzed did not present microbial contamination...

Avaliação microbiologica e fisico-quimica do polen apicola in natura e desidratado sob diferentes temperaturas; Evaluation microbiological and physico-chemical in nature the pollen and dried at different temperatures

Heloisa Litholdo Hervatin
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 23/09/2009 Português
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O pólen apícola é a aglutinação do pólen presente nas flores coletados pelas abelhas operarias, e depositados na entrada da colméia. O estudo visou avaliar a qualidade microbiológica e físico-química do pólen IN NATURA e do submetido a diferentes processos de secagem, simulando as condições de processamento do produto comercializado. Também avaliou as amostras adquiridas no comercio do estado de São Paulo. Registrou-se a escassez das pesquisas sobre a qualidade microbiológica do pólen apícola. Primeiramente a pesquisa, analisou amostras de pólen apícola obtidas através de coletores instalados nas colméias pertencentes ao apiário experimental do Pólo Regional de Desenvolvimento Tecnológico dos Agronegócios do Vale do Paraíba, localizado em Pindamonhangaba-SP, pertencente à Agência Paulista de Tecnologia dos Agronegócios. A coleta ocorreu nos intervalos de 24horas, 48horas e 72horas, objetivando a presença e/ou quantidade de microrganismos patogênicos no pólen com relação ao tempo de permanência do produto no campo. As amostras foram congeladas por 48horas, e submetidas a dois processos de secagem (circulação de ar em sistema aberto a 40-42°C e sistema de circulação de ar fechado a 30°C). Foram realizadas as análises físico-químicas para os parâmetros de pH...

Microbiological profile of salpicão de vinhais and chouriça de vinhais from raw materials to final products: Traditional dry sausages produced in the North of Portugal

Ferreira, V.; Barbosa, J.; Silva, J.; Gibbs, P.; Hogg, T.; Teixeira, P.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em //2009 Português
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Salpicão de Vinhais and Chouriça de Vinhais are traditional dry-fermented meat sausages produced in the North of Portugal. The microbiological profile during production of both products was followed in three small processing units. Lactic acid bacteria were the most prevalent microorganisms, with enhanced growth during the smoking process and a consequent decrease of pH. This study demonstrated that when highly contaminated raw ingredients are used, pathogens might not be eliminated during processing (from seasoning until the end of smoking). Therefore, it is important to highlight that to produce safe Salpicão and Chouriça following traditional recipes and methodologies it is necessary to work under hygienic conditions using raw materials of good microbiological quality. Industrial relevance: Preservation of pork meat in the form of semi-dry, fermented and smoked products having a long shelf-life, is an age-old artisanal process that is still practiced in many areas of the world. In many rural communities production has a significant impact on the local economy and food supply, but these products are now becoming increasingly popular in urban areas. In a more widespread distribution of such artisanal products, it is important that consumers are not exposed to undue risks of food-borne infections or intoxications. This study investigates the evolution of the microflora and microbiological safety during the production processes of two such traditional products in the North of Portugal...

Microbiological study of showerhead waters from 10 beaches of great Lisboa and West Regions of Portugal

Costa, Maria do Céu; Oliveira, Eva; Pires, Catarina; Moleiro, Filipa; Capitia, Vanessa; Vida, Manuela
Fonte: Edições Universitárias Lusófonas Publicador: Edições Universitárias Lusófonas
Tipo: Artigo de Revista Científica
Publicado em //2014 Português
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Many bacteria pathogenic to man can interfere in water distribution systems, forming aggregates that give rise to a complex structure called biofilm. In order to monitor the microbiological content of water from beach showers from ten sites along the coast from Lisboa-Carcavelos to Foz do Arelho beaches, 24 water samples were analyzed in 2013, 12 during January and February, and other 12 during June. The samples were collected in 500 mL sterile bottles with sodium thiosulfate. Biofilms were collected on swabs (at the same water sampling points) with transport medium Tween 20, and maintained between 2 ºC and 4 ° C, such as water samples. The following microbiological parameters were investigated for water samples: total microbial count at 22 ºC and 37 ºC, total coliforms and Escherichia coli, Pseudomonas aeruginosa, fecal streptococci, total Staphylococcus and coagulase-positive Staphylococcus. The swabs lead to the isolation of the microorganisms grown on the nutrient agar medium, in order to determine the the same microbiological parameters investigated in water showers. Globally, the water quality was found to be suitable, but that does not rule out the need for constant monitoring of the beach showers. Taking into account that there have been reports of plankton invasions and allergic eruptions after bathing in some seaside spots near Lisboa...

Building a Portuguese Food Microbiological Information Network

Viegas, Silvia; Machado, Claudia; Dantas, Maria; Oliveira, Luísa
Fonte: Instituto Nacional de Saúde Doutor Ricardo Jorge, IP Publicador: Instituto Nacional de Saúde Doutor Ricardo Jorge, IP
Tipo: Conferência ou Objeto de Conferência
Publicado em 18/05/2011 Português
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Introduction: The integration of food data from research, microbiological monitoring, epidemiological investigation and disease surveillance is crucial to manage foodborne risk. Consequently, INSA launched the Portuguese Food Information Resource Programme (PortFIR) in a partnership with GS1 Portugal to create national food chain expert networks and sustainable databases on food composition, consumption and chemical and microbiological contamination. Presently, the Food Microbiological Information Network (RPIMA) is being built. Purpose: The purpose is to build RPIMA including users and stakeholders, food microbiological data producers and regional authorities on agriculture and health to maximize resources (data, knowledge, financial, human, equipment…), spread knowledge and amplify the national capability. Methods: Potential users, stakeholders and data producers were identified and invited to participate in RPIMA through an e-mail questionnaire. A network meeting was organized to present, discuss and approve RPIMA’s goals. Structured brainstorming with experts of all food chain steps was used to define thematic working groups. Terms of Reference of PortFIR transversal working groups (WGs), “Users”, “Organization and Transfer of Information” and “Support to Standardization Work” were adapted to microbiology specificities through consensus within the WGs. Results: Currently the network has 82 members covering activity areas like food production and trade...

Avaliação de perigos microbiologicos no preparo de formulas infantis em lactario hospitalar.; Evaluation of microbiological hazards in the preparation of infant formulas in a hospital lactary.

Pamela Rossi
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 17/08/2007 Português
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As doenças transmitidas por alimentos (DTAs) colocam em risco o bem estar e a vida de muitas pessoas todos os dias, apresentando maior severidade em pessoas com estado de saúde mais debilitado, como crianças hospitalizadas. Para esta população alvo, uma das principais fontes de perigos é a alimentação preparada nos lactários dos hospitais. Assim é evidente a importância de um sistema preventivo de controle de qualidade que garanta a segurança dos alimentos fornecidos para esses pacientes. Este trabalho teve o objetivo de avaliar perigos microbiológicos existentes no preparo de fórmulas infantis em lactário de hospital público, a fim de adequar o estabelecimento aos pré-requisitos necessários para posterior implantação do sistema Análise de Perigos e Pontos Críticos de Controle (APPCC). Esta avaliação foi feita através do diagnóstico das condições higiênico-sanitárias do estabelecimento e do estudo do fluxograma de preparo da fórmula láctea infantil (FLI) reconstituída, onde foram estabelecidos dez pontos amostrais, nos quais foram realizadas análises microbiológicas do ar, de equipamento e utensílios e da FLI em pó e reconstituída. As amostras foram coletadas em seis lotes de preparação, compondo um total de 60 amostras às quais foram realizadas determinações de coliformes totais e fecais...

Increasing pathomorphism of pulmonary tuberculosis: an observational study of slow clinical, microbiological and imaging response of lung tuberculosis to specific treatment. Which role for linezolid?

Manfredi,Roberto; Nanetti,Anna; Monte,Paola Dal; Calza,Leonardo
Fonte: Brazilian Society of Infectious Diseases Publicador: Brazilian Society of Infectious Diseases
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2009 Português
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During recent years, a progressive emerging of tuberculosis occurred, related to the overall increased age of general population, primary and secondary (iatrogenic) immunodeficiencies, the availability of invasive procedures, surgical interventions and intensive care supports, bone marrow and solid organ transplantation, and especially the recent immigration flows of people often coming from areas endemic for tuberculosis, and living with evident social-economical disadvantages, and with a reduced access to health care facilities. Since January 2006, at our reference centre we followed 81 consecutive cases of pulmonary tuberculosis, with 65 of them which remained evaluable for the absence of extrapulmonary complications, and a continuative and effective clinical and therapeutic follow-up. The majority of episodes of evaluable pulmonary tuberculosis (49 cases out of 65: 75,4%) occurred in patients who immigrated from developing countries. In two patients multiresistant (MDR) Mycobacterium tuberculosis strains were found, while two more subjects (both immigrated from Eastern Europe) suffered from a disease due to extremely resistant (XDR) M. tuberculosis strains. Although enforcing all possible measures to increase patients' adherence to treatment (empowerment...

Relationship between the Agricultural Management of a Semi-arid Soil and Microbiological Quality

Moreno, J. L.; Bastida, F.; García Izquierdo, Carlos; Hernández Fernández Muñoz, María Teresa
Fonte: Taylor & Francis Publicador: Taylor & Francis
Tipo: Artículo Formato: 579917 bytes; image/jpeg
Português
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The establishment of the incidence of different agricultural management practices on soil microbiological quality has become an issue of wide concern. This study was aimed to (i) determine the effect of different agricultural management practices (ecological, integrated, conventional, and conventional with the addition of pig slurry) on the microbial activity of a semi-arid soil at two different stages (cropped and fallow) and (ii) adapt an existing quantitative method to assess the microbiological soil quality in the different management systems. Several microbial and biochemical parameters, as well as two water-soluble carbon (C) fractions, were measured in the soil samples. Significant differences (P<0.05) were found between the different agricultural management systems for most of the microbiological parameters during the cropped period. Factorial analysis indicated that adenosine triphosphate (ATP), microbial biomass C, diphenol oxidase activity, urease activity, and water-soluble carbohydrates had a high weight in factor 1. These parameters were selected to form part of the equation for calculating a microbiological quality index. During the cropped period, the ecological management yielded the highest score of the microbial quality index...

Reprodutibilidade de um teste microbiológico para estreptococos do grupo mutans; Reproducibility of a simplified microbiological test formutans streptococci

PINELLI, Camila; LOFFREDO, Leonor de Castro Monteiro; SERRA, Mônica Campos
Fonte: Universidade de São Paulo. Faculdade de Odontologia Publicador: Universidade de São Paulo. Faculdade de Odontologia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Artigo Avaliado pelos Pares Formato: application/pdf
Publicado em 01/03/2000 Português
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Com o desenvolvimento de testes microbiológicos salivares simplificados tornou-se mais prático o diagnóstico de pacientes com baixa atividade, porém com alto risco de cárie. O propósito desta pesquisa foi avaliar a reprodutibilidade do CARITEST-SM®, um teste específico para estreptococos do grupo mutans. Catorze voluntários participaram do estudo. A produção de saliva foi estimulada com uma goma de mascar inerte e coletada até a obtenção do volume suficiente para a execução de dois testes - pertencentes a dois conjuntos de lotes diferentes de "kits" CARITEST-SM® (1 e 2) - aplicados para um mesmo indivíduo. Dois examinadores calibrados realizaram a leitura de forma independente e cega, classificando os resultados de duas maneiras: escores de 1 a 6, conforme recomenda o fabricante, e conforme a classificação quanto à contagem microbiológica, baixa e alta. Foi aplicada a estatística Kappa, segundo Light. Os resultados de concordância, conforme 6 escores, mostraram valores distintos para o conjunto dos "kits" 1 e para o conjunto dos "kits" 2, valendo, respectivamente, kapa = 0,57 e kapa = 0,21. Em relação à classificação conforme a contagem microbiológica, alta e baixa, os resultados foram kapa = 1,00, para o conjunto dos "kits" 1...

Microbiological and physicochemical analysis of yateí (Tetragonisca angustula) honey for assessing quality standards and commercialization

Pucciarelli,Amada B; Schapovaloff,María E; Kummritz,Silvana; Señuk,Isabel A; Brumovsky,Luis A; Dallagnol,Andrea M
Fonte: Revista argentina de microbiología Publicador: Revista argentina de microbiología
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2014 Português
Relevância na Pesquisa
36.75%
Due to the interest in the production and trading of yateí (Tetragonisca angustula) honey in the province of Misiones, Argentina, in this work we assessed microbiological and physicochemical parameters in order to contribute to the elaboration of standards for quality control and promote commercialization. Results showed that yateí honey samples had significantly different microbiological and physicochemical characteristics in comparison to established quality standards for Apis mellifera honey. Thus, we observed that values for pH (3.72), glucose (19.01 g/100 g) and fructose (23.74 g/100 g) were lower than A. mellifera quality standards, while acidity (79.42 meq/kg), moisture (24%), and mould and yeast count (MY) (3.02 log CFU/g) were higher. The acid content was correlated with glucose (R²=0.75) and fructose (R²=0.68) content, and also with mould and yeast counts (R²=0.45) to a lesser extent. The incidence of microorganisms in yateí honey samples reached 42.85% and 39% for Clostridium sulfite-reducers and Bacillus spp., respectively. No C. botulinum or B. cereus cells were detected. Enterococcus spp. and Staphylococcus spp. incidence was similar (ca. 7.14%), whereas Escherichia coli and Salmonella spp. were not detected. We conclude that the microbiological and physicochemical properties of yateí honey are different from those of A. mellifera honey; hence...

Assessment of the origin of microbiological contamination of groundwater at a rural watershed in Chile

Valenzuela,Mariela; Mondaca,María A.; Claret,Marcelino; Pérez,Claudio; Lagos,Bernardo; Parra,Oscar
Fonte: Colegio de Postgraduados Publicador: Colegio de Postgraduados
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2009 Português
Relevância na Pesquisa
36.75%
In a rural watershed in Chile, the scarce groundwater available represents almost the only water source both for agriculture and domestic use. This water has microbiological quality problems, which result in an agricultural and local economic development constraint. Contamination can come from punctual or diffuse sources. Characterizing the microbiological quality of groundwater allows both to identify sources from the point of view of whether they are point or non point -thus facilitating their reduction or elimination- and to determine health hazards likely to affect the population in the area under study. This study aimed to improve the state of knowledge on the microbiological quality of groundwater at a rural watershed. Forty two wells were seasonally analyzed over a one-year period. The indicators microorganisms -total coliforms, fecal coliforms and fecal streptococci- were quantified. The study of the probable origin of the indicators was undertaken using the fecal coliform to fecal streptococci ratio, biochemical identification of enteric bacteria, and somatic coliphages detection as presence of human enteric virus indicator. Temporal contamination dynamics was determined with statistical analysis of indicator organism concentration. Results suggest that the main source of fecal contamination is of animal origin...