Página 1 dos resultados de 6175 itens digitais encontrados em 0.011 segundos
Resultados filtrados por Publicador: Pontifícia Universidade Católica do Rio Grande do Sul

Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry; Avaliação da qualidade do DNA extraído de saliva humana armazenada e sua aplicabilidade na identificação em Odontologia Legal

CARVALHO, Suzana Papile Maciel; SALES-PERES, Arsenio; RIBEIRO-BICUDO, Lucilene Arilho; SILVA, Ricardo Henrique Alves da
Fonte: Pontifícia Universidade Católica do Rio Grande do Sul Publicador: Pontifícia Universidade Católica do Rio Grande do Sul
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
37.12%
PURPOSE: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. METHODS: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was extracted from the 40 saliva samples and subjected to PCR and electrophoresis. After 180 days, the technique was repeated with the 20 swab samples. RESULTS: The first-stage results indicated that DNA was successfully extracted in 97.5% of reactions, 95% of saliva in natura and 100% of swab saliva samples, with no statistically significant difference between the forms of saliva. In the second phase, the result was positive for all 20 analyzed samples (100%). Subsequently, in order to analyze the quality of the DNA obtained from human saliva, the SIX3-2 gene was tested on the 20 mouth swab samples, and the PCR products were digested using the MbO1 restriction enzyme to evaluate polymorphisms in the ADRA-2 gene, with positive results for most samples. CONCLUSION: It was concluded that the quantity and quality of DNA from saliva and the techniques employed are adequate for forensic analysis of DNA.; OBJETIVO: Este trabalho objetivou avaliar a qualidade do DNA obtido de saliva humana armazenada e sua aplicabilidade da identificação de pessoas. METODOLOGIA: Analisaram-se amostras salivares de n=20 sujeitos da pesquisa...