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Efeito do uso de goma de mascar contendo xilitol sobre os níveis salivares de Estreptococos do grupo mutans, sobre os genótipos de S. mutans e sobre a presença de amostras xilitol-tolerantes na saliva.; Effect of xylitol-containing chewing gum consumption on salivary mutans streptococci levels, on genotypes of S.mutans and on the presence of xylitol-resistant strains in saliva.

Trindade, Claudia Perez
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 01/02/2006 Português
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Este trabalho teve o objetivo de avaliar o efeito do uso de goma de mascar contendo 15% de xilitol, por 30 dias, em 12 indivíduos com altos níveis salivares (= 10 5 u.f.c./ml de saliva) de Estreptococos do grupo mutans (SM) sobre os níveis salivares de SM, os genótipos de S. mutans e a ocorrência de amostras xilitol-tolerantes (X R ) na saliva. Os voluntários foram instruídos a mascarem a goma por 5 minutos, 5 vezes ao dia, durante 30 dias, sem realizarem nenhuma alteração nos hábitos de higiene ou dieta. Amostras de saliva foram coletadas no início do experimento (antes do consumo da goma de mascar), 30 dias após a utilização da goma de mascar e 30 dias após a interrupção do uso da goma. Dois voluntários foram excluídos da última coleta devido ao uso de antibióticos. Os resultados mostraram que ocorreu redução acentuada nos níveis salivares de SM. A concentração média de SM no exame inicial foi de 9,8 x 10 5 ± 2,61 x 10 5 UFC/ml de saliva, enquanto este valor foi de 2,17 x 10 5 ± 1,94 x 10 5 após o consumo da goma, sendo a diferença estatisticamente significante (p < 0,05). Porém este efeito não foi duradouro, não apresentando diferença estatística 30 dias após a interrupção do consumo (3,5 x 10 5 ± 2...

Fatores de risco associados à cárie dentária e ao alto nível de estreptococos mutans em crianças de 12 a 24 meses em creches do município de São Paulo - SP; Risk factors of Dental carie and high levels of Mutans streptococci in children aged 12-24 months old in day care centers of São Paulo, SP

Pereira, Daniela Forlin
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 30/03/2007 Português
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O objetivo deste estudo foi avaliar, em crianças de creches públicas e filantrópicas do município de São Paulo, a prevalência de cárie dental e os fatores de risco relacionados à presença de cárie e presença de altos níveis de estreptococos mutans (SM). Foram incluídas variáveis denominadas sociodemográficas, condições de gestação e nascimento, variáveis fisiológicas do estado nutricional, aleitamento materno e alimentação complementar, hábitos alimentares, variáveis comportamentais e odontológicas. O estudo foi desenvolvido em parceria entre a Disciplina de Odontopediatria da Faculdade de Odontologia da Universidade de São Paulo (FOUSP) e a Disciplina de Nutrologia do Departamento de Pediatria da Universidade Federal de São Paulo (UNIFESP EPM). É parte integrante do Projeto Crecheficiente - Ações de Segurança e Educação Alimentar em Creches Públicas e Filantrópicas do Município de São Paulo. A população deste estudo foi constituída de 150 e 144 crianças, que participaram da avaliação odontológica e microbiológica respectivamente, entre 12 e 24 meses, de ambos os gêneros, regularmente matriculadas em cinco creches, sendo duas públicas e três filantrópicas. A coleta dos dados foi obtida por meio de entrevistas com as mães ou responsáveis...

Group B streptococci colonization in pregnant women: risk factors and evaluation of the vaginal flora

Rocchetti, Talita T.; Marconi, Camila; Rall, Vera L. M.; Borges, Vera T. M.; Corrente, Jose E.; da Silva, Marcia
Fonte: Springer Heidelberg Publicador: Springer Heidelberg
Tipo: Artigo de Revista Científica Formato: 717-721
Português
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 06/55307-0; Processo FAPESP: 07/51704-7; To determine the prevalence of group B streptococci (GBS) in our population, and to assess the association between risk factors and vaginal flora with maternal rectovaginal colonization.Samples were obtained from 405 patients between 35 and 37 weeks of gestation. Swabs from the vaginal and perianal regions were cultured in Todd Hewitt and subcultured in blood agar. Colonies suggestive of GBS were submitted to catalase and CAMP test. The vaginal flora was evaluated on Gram stain vaginal smears. Socio-demographic and obstetric data were obtained by designed form. Considering maternal GBS colonization as the response variable, a logistic regression model was fitted by the stepwise method with quantitative and qualitative explanatory variables.The prevalence of GBS colonization was 25.4%. The most frequent vaginal flora abnormalities were cytolytic vaginosis (11.3%), followed by bacterial vaginosis (10.9%), candidosis (8.2%) and intermediate vaginal flora II (8.1%). Logistic regression analysis revealed that maternal age, number of sexual intercourse/week, occurrence of previous spontaneous abortion, presence of candidosis and cytolytic vaginosis were associated with streptococcal colonization.The prevalence of GBS is high in pregnant women and is associated with sexual intercourse frequency...

Effect of Cervitec (R) on mutans streptococci in plaque and on caries formation on occlusal fissures of erupting permanent molars

Araujo, AMPG; Naspitz, GMCC; Chelotti, A.; Cai, S.
Fonte: Karger Publicador: Karger
Tipo: Artigo de Revista Científica Formato: 373-376
Português
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The aim of this study was to evaluate the effect of Cervitec(R) on the abundance of mutans streptococci (MS) in occlusal dental plaque and on 2-year caries increment of partly erupting first permanent molars. Sixteen healthy schoolchildren aged 6-8 years, with at least 2 sound contralateral partly erupted permanent molars, received diet counselling and daily parental supervised toothbrushing with a fluoride dentifrice. Stimulated saliva samples were collected at baseline and after 1 year to evaluate MS levels. In a split-mouth design, Cervitec varnish was applied to one of the teeth at baseline and after 3 and 6 months, while the other tooth in the same jaw was a control. At the 9-month follow-up the teeth were in occlusal contact. At this time, varnish was not applied. At 3 and 6 months after the first application of varnish a significant suppression of MS was observed in plaque. Caries investigations, performed at baseline and every 3 months during the 2 years after the start of the study, showed that all the teeth treated with the varnish were free of caries after 2 years, whereas 8/16 control teeth developed incipient caries. In conclusion, our results suggest that treatment with Cervitec reduces MS in plaque on erupting permanent molars and can lead to a significant decrease in caries incidence. Copyright (C) 2002 S. Karger AG...

Association of free amino acids with caries experience and mutans streptococci levels in whole saliva of children with early childhood caries

Fonteles, Cristiane S. R.; Guerra, Milene H.; Ribeiro, Thyciana R.; Mendonca, Danielle N.; de Carvalho, Cibele B. M.; Monteiro, Andre J.; Toyama, Daniela O.; Toyama, Marcos H.; Fonteles, Manasses C.
Fonte: Pergamon-Elsevier B.V. Ltd Publicador: Pergamon-Elsevier B.V. Ltd
Tipo: Artigo de Revista Científica Formato: 80-85
Português
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Objective: The aim of the present study was to identify the free amino acid content in whole saliva of children with (CE) and without early childhood caries (CF) (ECC), correlating these findings with caries experience and mutans streptococci (MS) levels in saliva.Design: Seventy-eight healthy children, both genders, 6-71 months age, were selected to participate in the study. Following examination for dmft scores calculation, unstimulated whole saliva was collected from all 78 participants, stored at -80 degrees C, and used for amino acid analysis, on a Biochem 20 plus amino acid analyzer. Stimulated whole saliva was collected from 52 children, transported, diluted and plated on MSB agar medium for detection of MS in cfu/mL.Results: Forty different free amino acids were identified in whole saliva, with great variation in their concentration. A statistically significant relation was found between caries experience and the presence of free proline and glycine. While proline (p = 0.0182) was more frequently absent in the CF group, the absence of glycine (p = 0.0397) was more often observed in the CE group. In the presence of higher levels of MS, free glycine reduced the risk of experiencing dental caries (p = 0.0419). Conversely, the presence of proline was found to increase the risk of experiencing the disease (p = 0.0492).Conclusions: The presence of free proline and absence of free glycine in children with ECC...

The effect of chlorhexidine on plaque index and mutans streptococci in orthodontic patients: A pilot study

Faria, Gisele; Santamaria Jr, Milton; Dos Santos, Bianca Mota; Ito, Izabel Yoko; Bregagnoloq, Janete Cinira; Sasso Stuani, Maria Bernadete
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 323-328
Português
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Aim: The purpose of this study was to assess chlorhexidine effects on plaque index and salivary levels of mutans streptococci (MS) when used as the immersion solution for removable orthodontic appliances and added to their acrylic resin composition. Methods: Forty-five patients (6 to 12 years old) were randomly assigned into three groups with 15 patients each. Group I (control)—without orthodontic appliances disinfection; Group II—removable orthodontic appliances which had been immersed in 0.12% chlorhexidine digluconate overnight (8 hours), and Group III—orthodontic appliances in which 0.12% chlorhexidine digluconate solution had been incorporated into their resin composition. Saliva was collected for quantification of MS and evaluation of plaque index was performed before and after installation of orthodontic appliance at 0, 2, 4, 6, 8, and 10 weeks. Data were analyzed by using analysis of variance. Results: Number of MS colonies in saliva and plaque index showed no statistically differences among groups at the different periods (p > 0.05). Conclusions: It could be concluded that chlorhexidine incorporation into the acrylic resin of removable orthodontic appliances at 0.12% concentration and immersion of the appliance into 0.12% chlorhexidine solution were not effective in reducing plaque index and the number of MS in saliva.

Cell surface hydrophobicity and adherence of a strain of group B streptococci during the post-antibiotic effect of penicillin

Araújo,Ângela Maria Mendes; Oliveira,Ivi Cristina Menezes de; Mattos,Marcos Corrêa de; Benchetrit,Leslie C.
Fonte: Instituto de Medicina Tropical Publicador: Instituto de Medicina Tropical
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2008 Português
Relevância na Pesquisa
36.66%
The minimum inhibitory concentration and post-antibiotic effects of an antimicrobial agent are parameters to be taken into consideration when determining its dosage schedules. The in vitro post-antibiotic effects on cell surface hydrophobicity and bacterial adherence were examined in one strain of group B streptococci. Exposure of the microorganism for 2 h at 37 °C to 1 x MIC of penicillin induced a PAE of 1.1 h. The cell surface charge of the Streptococcus was altered significantly during the post-antibiotic phase as shown by its ability to bind to xylene: hydrophobicity was decreased. Bacterial adherence to human buccal epithelial cells was also reduced. The results of the present investigation indicate that studies designed to determine therapeutic regimens should evaluate the clinical significance of aspects of bacterial physiology during the post-antibiotic period.

Penicillin tolerance among Beta-hemolytic streptococci and production of the group carbohydrates, hemolysins, hyaluronidases and deoxyribonucleases

Avelino,Cássia C.; Benchetrit,Leslie C.
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/1995 Português
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Penicillin tolerance among 67 strains of beta-hemolytic streptococci was examined by determining the ratio of the minimal bactericidal concentration to the minimal inhibitory concentration as 32 or greater. Tolerance was demonstrated in 15 group A strains and in 11,7, and 4 of groups B, C and G, respectively. Thereafter the effects of a subminimal inhibitory concentration (1/2MIC) of penicillin on the bacterial products of four tolerant and four nontolerant strains (two of each Lancefield group) were analyzed and compared. The antibiotic caused a marked increase in the expression of the group carbo-hydrates for strains of group B. Penicillin was found to reduce the cell-bound hemolysin activities of the four tolerant strains and to increase the activity of the other (free) form of nontolerant groups A, C and G hemolysins. Penicillin caused an increase in the extracellular hyaluronidase activities of one group A and groups B, C and G streptococci. With added antibiotic the production of deoxyribonuclease by tolerant groups A, C and G was greatly enhanced and that of the group B streptococcus was arrested.

Pulsed-field gel electrophoresis, virulence determinants and antimicrobial susceptibility profiles of type Ia group B streptococci isolated from humans in Brazil

Corrêa,Ana Beatriz de A; Oliveira,Ivi Cristina M de; Pinto,Tatiana de CA; Mattos,Marcos C de; Benchetrit,Leslie C
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/07/2009 Português
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Group B streptococci (GBS) infections occur worldwide. Although serotyping has been used for epidemiologic purposes, this does not accurately characterize enough members of a genetically heterogeneous bacterial population. The aims of this work were to evaluate the genetic diversity of 45 type Ia GBS strains isolated in Brazil by pulsed-field gel electrophoresis as well as to evaluate antimicrobial susceptibility profiles and identify virulence genes. Twenty-four strains were assigned to cluster A. All strains under study contained the hylB and scpB genes. The bca gene was detected in only 10 strains and none of the streptococci carried the bac gene. Thirty-nine strains were resistant to tetracycline.

Detection of oral streptococci in dental biofilm from caries-active and caries-free children

Silva,Andréa Cristina Barbosa da; Cruz,Jader dos Santos; Sampaio,Fábio Correia; Araújo,Demetrius Antônio Machado de
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2008 Português
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This work correlated the presence of oral streptococci in dental biofilm with clinical indexes of caries and oral hygiene in caries-active and caries-free children. S. mutans and/or S. sobrinus in the dental biofilm does not indicate a direct risk for developing dental caries.

Mediation of adherence of streptococci to human endothelial cells by complement S protein (vitronectin).

Valentin-Weigand, P; Grulich-Henn, J; Chhatwal, G S; Müller-Berghaus, G; Blobel, H; Preissner, K T
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1988 Português
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The role of S protein in the adherence of group A and G streptococci to human umbilical vein endothelial cells cultivated in 96-well microdilution plates was studied by utilizing fluorescein-labeled streptococci. The assay proved suitable for quantitative determination of bacterial adherence to cultured endothelial cells for all tested strains of streptococci. Only bacterial strains with significant S protein binding but weak fibronectin binding were included in these studies. Fibronectin-mediated adherence to endothelial cells of these streptococci was less than 25% of total and could be blocked by antifibronectin immunoglobulin G. Further treatment of endothelial cell monolayers with anti-S protein immunoglobulin G at concentrations up to 1 mg per well led to an almost complete inhibition of adherence for all tested streptococcal cultures, indicating significant contribution of S protein in the streptococcus-endothelial cell interaction. Blocking of S-protein-binding sites on streptococci by preincubation with exogeneous S protein at a concentration of 10 micrograms per 4 x 10(7) streptococci led to about 75% reduction of S-protein-mediated adherence to endothelial cells. Trypsin pretreatment of group G streptococci and pronase pretreatment of group A and G streptococci...

Specific binding of the human S protein (vitronectin) to streptococci, Staphylococcus aureus, and Escherichia coli.

Chhatwal, G S; Preissner, K T; Müller-Berghaus, G; Blobel, H
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /08/1987 Português
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Specific binding of the 125I-labeled human S protein (vitronectin) which has been shown to be identical with serum-spreading factor, was observed with group A, C, and G streptococci as well as with Staphylococcus aureus and Escherichia coli. The specific binding of S protein to group A, C, and G streptococci was high, whereas the binding to S. aureus and E. coli cultures was moderate. In contrast, group B streptococci and a number of other bacterial species tested did not interact with S protein. The binding of S protein to bacteria was saturable and could be inhibited only by unlabeled S protein but not by albumin. Trypsinization and heat treatment of bacteria destroyed the S-protein binding capacity for group G streptococci, S. aureus, and E. coli but not for group A and C streptococci. Likewise, unlabeled human fibronectin and heparin inhibited the binding of labeled S protein to group G streptococci, S. aureus, and E. coli, but did not influence the binding to group A and C streptococci. Double-reciprocal plots of S-protein binding to group G streptococci indicated that fibronectin inhibited the binding in a competitive manner, while heparin acts in a noncompetitive manner. Moreover, the binding of S protein to G streptococci could be partially by the synthetic peptide Gly-Arg-Gly-Asp-Ser...

Presumptive Identification of Group A, B, and D Streptococci

Facklam, R. R.; Padula, J. F.; Thacker, L. G.; Wortham, E. C.; Sconyers, B. J.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /01/1974 Português
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A battery of five tests was used for presumptive identification of the pathogenic streptococci. The non-serological methods included determination of hemolysis for all strains, bacitracin susceptibility for group A streptococci, hippurate hydrolysis by group B streptococci, and bile-esculin reaction for group D streptococci. Enterococcal group D streptococci were differentiated from non-enterococcal group D streptococci by 6.5% NaCl tolerance. Two other categories of streptococci resulted: beta-hemolytic streptococci non-groups A, B, or D; and alpha- or nonhemolytic streptococci, not enterococci, not further identified (viridans streptococci). The tests were used as a battery and not as single entities. In this manner more than 99% of the group A, 99% of the group B, 81% of the beta-hemolytic streptococci non-group A, B, or D, 99% of the group D enterococci, 97% of the group D non-enterococci, and 94% of the viridans streptococci were correctly identified.

Factors influencing oral colonization of mutans streptococci in young children

Law, V.; Seow, W.; Townsend, G.
Fonte: Australian Dental Assn Inc Publicador: Australian Dental Assn Inc
Tipo: Artigo de Revista Científica
Publicado em //2007 Português
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This paper aims to critically review current knowledge about the key factors involved in oral colonization of the cariogenic group of bacteria, mutans streptococci (MS) in young children. MS, consisting mainly of the species Streptococcus mutans and Streptococcus sobrinus, are commonly cultured from the mouths of infants, with prevalence of infection ranging from around 30 per cent in 3 month old predentate children to over 80 per cent in 24 month old children with primary teeth. MS is usually transmitted to children through their mothers, and the risk of transmission increases with high maternal salivary levels of MS and frequent inoculation. Factors that affect the colonization of MS may be divided into bacterial virulence, hostrelated and environmental factors. Complex interaction among these factors determine the success and timing of MS colonization in the child. As clinical studies have shown that caries risk is correlated with age at which initial MS colonization occurred, strategies for the prevention of dental caries should include timely control of colonization of the cariogenic bacteria in the mouths of young children.; V Law, WK Seow and G Townsend; The document attached has been archived with permission from the Australian Dental Association. An external link to the publisher’s copy is included.

Avaliação da resistencia a antimicrobianos de Staphylococcus aureus e estreptococos grupo Viridans de pacientes com risco a endocartide infecciosa; Evaluation of antimicrobial resistance os Staphylococcus aureus and viridans streptococci of patients at risk for infecrive endocarditis

Roberta Cristiane Catelli Baglie
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 24/03/2006 Português
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37.02%
A endocardite infecciosa (EI) é uma infecção grave das válvulas cardíacas, do endocárdio mural e de septos defeituosos, provocada principalmente pelos Staphylococcus aureus e pelos estreptococos grupo viridans. O objetivo deste trabalho foi verificar a resistência aos antimicrobianos de cepas de Staphylococcus aureus e estreptococos grupo viridans provindas de 100 voluntários, sendo 60 adultos (16 a 75 anos) que foram divididos em três grupos: saudáveis (grupo 1), cardiopatas de baixo risco (grupo 2) e cardiopatas de alto risco (grupo 3) para EI e 40 crianças (6 a 14 anos) que foram divididas em dois grupos: saudáveis (grupo 1) e cardiopatas de alto risco (grupo 2) para EI. Além disso, os voluntários dos grupos 2 e 3 e os responsáveis pelas crianças do grupo 2 responderam a um formulário sobre os riscos da EI e sua profilaxia. Amostras de saliva (diluídas 1: 1000) foram inoculadas em Mitis Salivarius agar e incubadas em microaerofilia durante 48 h. Amostras de pele (swab) foram inoculadas em Sal Manitol agar e incubadas em aerobiose durante 24 h. Após testes bioquímicos, os estreptococos grupo viridans e os Staphylococcus aureus foram identificados e submetidos a testes de susceptibilidade microbiológica empregando-se agentes antimicrobianos. Dos voluntários adultos...

STREPTOCOCCI IN INFECTIOUS (ATROPHIC) ARTHRITIS AND RHEUMATIC FEVER

Nye, Robert N.; Waxelbaum, Edgar A.
Fonte: The Rockefeller University Press Publicador: The Rockefeller University Press
Tipo: Artigo de Revista Científica
Publicado em 30/11/1930 Português
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The question of the relationship of streptococci to the etiology of infectious arthritis and of rheumatic fever is of the utmost importance. If a streptococcus or group of streptococci could be shown to be associated See PDF for Structure with either disease, some form of specific treatment might be available. The possibility of primary streptococcic infection as the cause of rheumatic fever, and, to a less extent, of acute infectious arthritis would seem to be a reasonable conjecture because of the frequency of associated throat, sinus or other focal infection. To consider that these same streptococci remain in or about the affected joint and to such an extent that they are found in the blood stream in cases of chronic infectious arthritis of years' duration demands a rather unique conception. Recent investigative work has certainly tended to confirm the importance of streptococci in these diseases, but, if all the published reports are considered as a group, one can not help being impressed with the inconsistency and peculiarities of the findings. In blood cultures from cases of rheumatic fever Clawson (7) recovered Streptococcus viridans, Small (8) and Birkhaug (9) non-hemolytic (gamma type) streptococci, and Cecil et al. (3) Streptococcus viridans...

Recolonization of Mutans Streptococci after Application of Chlorhexidine Gel

Vale,Glauber Campos; Cury,Altair Antoninha Del Bel; Arthur,Rodrigo Alex; Cury,Jaime Aparecido; Tabchoury,Cínthia Pereira Machado
Fonte: Fundação Odontológica de Ribeirão Preto Publicador: Fundação Odontológica de Ribeirão Preto
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2014 Português
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Streptococcus mutans is specifically suppressed by intensive treatment with chlorhexidine gel, but the time for recolonization and the effect on other oral bacteria are not totally clear. In this study, recolonization of mutans streptococci was evaluated in nine healthy adult volunteers, who were highly colonized with this microorganism. Stimulated saliva was collected before (baseline) and at 1, 7, 14, 21 and 28 days after application of 1% chlorhexidine gel on volunteers' teeth for two consecutive days. On each day, the gel was applied using disposable trays for 3 x 5 min with intervals of 5 min between each application. Saliva was plated on blood agar to determine total microorganisms (TM); on mitis salivarius agar to determine total streptococci (TS) and on mitis salivarius agar plus bacitracin to determine mutans streptococci (MS). Chlorhexidine was capable of reducing the counts of MS and the proportion of MS with regard to total microorganisms (%MS/TM) (p<0.05), but these values did not differ statistically from baseline (p>0.05) after 14 days for MS and 21 days for %MS/TM. The counts of TM and TS and the proportion of MS to total streptococci did not differ statistically from baseline (p>0.05) after chlorhexidine treatment. The results suggest that the effect of chlorhexidine gel treatment on suppression of mutans streptococci is limited to less than a month in highly colonized individuals.

Hidrofobicidade de superfície celular e aderência de uma estirpe de estreptococos do grupo B durante o efeito pós-antibiótico da penicilina; Cell surface hydrophobicity and adherence of a strain of group B streptococci during the post-antibiotic effect of penicillin

Araújo, Ângela Maria Mendes; Oliveira, Ivi Cristina Menezes de; Mattos, Marcos Corrêa de; Benchetrit, Leslie C.
Fonte: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo Publicador: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/08/2008 Português
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A concentração mínima inibitória e os efeitos pós-antibióticos (EPA) de um agente antimicrobiano são parâmetros que devem ser levados em consideração quando da determinação do esquema de dosagem. Os efeitos pós-antibióticos in vitro na hidrofobicidade de superfície celular e na aderência foram pesquisados em uma amostra de estreptococos do grupo B. A exposição do microrganismo por 2 h a 37 °C a 1 x CMI de penicilina induziu um EPA de 1,1 h. A carga da superfície celular da bactéria foi alterada significativamente durante a fase pós-antibiótica revelada através da capacidade de ligação ao xileno, indicada pela diminuição da hidrofobicidade. A aderência bacteriana às células epiteliais bucais humanas também foi reduzida. Os resultados da investigação demonstram que estudos clínicos destinados a determinar regimes terapêuticos deveriam incluir o conhecimento da fisiologia bacteriana durante o período pós-antibiótico.; The minimum inhibitory concentration and post-antibiotic effects of an antimicrobial agent are parameters to be taken into consideration when determining its dosage schedules. The in vitro post-antibiotic effects on cell surface hydrophobicity and bacterial adherence were examined in one strain of group B streptococci. Exposure of the microorganism for 2 h at 37 °C to 1 x MIC of penicillin induced a PAE of 1.1 h. The cell surface charge of the Streptococcus was altered significantly during the post-antibiotic phase as shown by its ability to bind to xylene: hydrophobicity was decreased. Bacterial adherence to human buccal epithelial cells was also reduced. The results of the present investigation indicate that studies designed to determine therapeutic regimens should evaluate the clinical significance of aspects of bacterial physiology during the post-antibiotic period.

; Prevalence of different streptococci species in the oral cavity of children and adolescents

Amoroso, Patricia; Avila, Fernando A.; Gagliardi, Célia M. O.
Fonte: UNICAMP/FOP Publicador: UNICAMP/FOP
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ;
Publicado em 11/11/2015 Português
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; Streptococci species were isolated, identified and counted in 262 saliva samples collected from 131 children and adolescents from a public school nursery at the city of Jaboticabal, SP. Four Streptococci species harboring oral samples were identified biochemically, as Streptococcus salivarius, Streptococcus mutans, Streptococcus rattus, Streptococcus sobrinus, and streptococci of the mitis group. The mean Streptococcus counts obtained were 1.0 x 109 CFU/ml saliva for the 3 to 5 year age range, 1.5 x 109 CFU/mL saliva for the 6 to 8 year age range, and 2.0 x 109 CFU/mL saliva for the 12 to 14 year age range. The prevalence of Streptococci in saliva were: S. salivarius (89.31%), S. mutans (73.28%), S. salivarius + S. mutans (44.27%) by the standard method. The tongue depressor method showed S. mutans (62.59%), S. salivarius (77.86%), S.salivarius + S. mutans (33.58%). The analysis of both techniques showed significant agreement for Streptococcus isolation.

; Effect of chlorhexidine gel containing saccharin or aspartame in deaf children highly infected with mutans streptococci

Fernandes, Frederico Silva de Freitas; Fernandes, Juliana de Kássia Braga; Marques, Sirlei Garcia; Silva, Rubenice Amaral da
Fonte: UNICAMP/FOP Publicador: UNICAMP/FOP
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; Formato: application/pdf
Publicado em 12/11/2015 Português
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; Aim: Since deaf children are unable to comprehend or cooperate with dental treatment due to lack of communication, preventive measures may be an important way to control the high prevalence of dental caries in these patients. The aim of the this study was to evaluate the effect of an intensive treatment with chlorhexidine (CHX) gel, containing either saccharin or aspartame, in deaf children highly infected with mutans streptococci (MS). Methods: Eighteen children were randomly divided into two groups, according to the sweetener used to improve the CHX gel bitter taste: saccharin or aspartame. Before CHX treatment, saliva samples were collected to establish baseline microbial data for MS. CHX gel was applied on two consecutive days, four times the first day and three times the second day. Saliva samples were then taken after 7, 30, 60, 90 and 120 days to evaluate MS oral recolonization. Results: CHX gel containing saccharin was not effective on the reduction of MS levels, while the gel containing aspartame decreased significantly MS levels after treatment (P<.05). Conclusions: Although a new CHX application may be necessary after 60 days to control caries risk and MS levels, CHX treatment should be individually controlled because of variations in the response of subjects.