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Efeitos clastogênicos causados por vários sorotipos de Ureaplasma urealyticum e espécies do gênero Mycoplasma sobre culturas temporárias de linfócitos; Clastogenic effects of different Ureaplasma urealyticum serotypes and species of Mycoplasma on temporary lymphocites cultures

Cunha, Regina Ayr Florio da
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 19/07/1993 Português
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Os efeitos clastogênicos causados por diferentes cepas Ureaplasma urealyticum e por cepas do gênero Mycoplasma foram avaliados "in vitro", utilizando-se culturas temporárias de linfócitos humanos. Inibições, total ou parcial da mitose foram produzidos pelos sorotipos II, III e X de Ureaplasma urealyticum, independentemente das concentrações dos inóculos de micoplasmas utilizados. Já os sorotipos I, VII e XII do Ureaplasma urealyticum, a cepa Mycoplasma hominis ATCC 23114 e a cepa Mycoplasma pneumoniae foram influenciadas pela concentração dos micoplasmas. Mitoses alteradas foram observadas nas cepas Ureaplasma urealyticum VIII, IX e X. A maior frequência de alterações foram de gaps cromatídicos e quebras cromatídicas. Os resultados obtidos neste modelo experimental "in vitro" revelaram haver comportamentos diferentes entre os vários sorotipos de Ureaplasma urealyticum e entre espécies do gênero Mycoplasma. Essas diferenças observadas, "in vitro", poderão de alguma forma contribuir para melhor compreensão dos efeitos da colonização desses microorganismos em humanos.; Clastogenic effect caused by different strains of Ureaplasma urealyticum and by strains of Mycoplasma sp were evaluated in vitro by using temporary cultures of human linphocytes. Mitotic inhibitions...

Prevalência da infecção por ureaplasma urealyticum e parvum em recém-nascidos de muito baixo peso

Fonseca, Luciana Teixeira
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Dissertação Formato: application/pdf
Português
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Introdução: Há tempos Micoplasmas Genitais como o Ureaplasma vêm sendo implicados na patogênese de trabalho de parto prematuro e morbidade neonatal, mas seu real papel permanece obscuro e sua prevalência no sangue de recém-nascidos de muito baixo peso ainda não foi estudada em nosso meio. Objetivo: Determinar a prevalência da infecção por Ureaplasma urealyticum (Uu) e Ureaplasma parvum (Up) em uma amostra de recém-nascidos de muito baixo peso (RNMBP) e avaliar os fatores associados. Pacientes e métodos: Foi realizada extração de DNA de amostras de sangue de RNMBP coletadas nas primeiras 72 horas de vida e a presença de Uu e/ou Up foi identificada por técnica de Reação em Cadeia de Polimerase (PCR). Os recém-nascidos foram acompanhados até a alta hospitalar. Resultados: Noventa e cinco recém-nascidos de muito baixo peso foram incluídos no estudo. A detecção de Uu e/ou Up ocorreu em 12 recém-nascidos (12,63%). Em 5,26% foi detectado somente Uu, em 5,26% somente Up e em 2,11% ambos. Na análise univariada a presença de Ureaplasma foi associada à infecção ovular e a trabalho de parto prematuro. Pré-eclâmpsia e ser PIG foram associados a menor ocorrência de Ureaplasma. Quando analisados apenas os nascimentos decorrentes de trabalho de parto prematuro...

AGAR MEDIUM FOR HUMAN UREAPLASMA UREALYTICUM

Raddi, MSG; Lorencetti, N. C.; Rossini, A. J.
Fonte: Soc Brasileira Microbiologia Publicador: Soc Brasileira Microbiologia
Tipo: Artigo de Revista Científica Formato: 208-209
Português
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67.42%
The authors wish to report a simple medium for the isolation and cultivation of Ureaplasma urealyticum starting from clinical materials. This medium induced growth in eleven of the twelve different serotypes of Ureaplasma urealyticum studied.

First-void versus midstream urine culture for detection of Ureaplasma urealyticum in the urinary tract

Raddi, MSG; Lorencetti, N. C.; Rodrigues, S. A.
Fonte: Soc Brasileira Microbiologia Publicador: Soc Brasileira Microbiologia
Tipo: Artigo de Revista Científica Formato: 200-202
Português
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67.42%
Quantitative Ureaplasma urealyticum culture was performed on clean first-void and midstream urine to evaluate the presence of these mollicutes in the urinary tract. The results, expressed as color changing units (CCU), showed that 14 (63%) of the 22 Ureaplasma urealyticum positive patients yielded counts equal to or higher that 10(7) CCU/mL for both the initial and the middle urine specimens. No abnormal chemical or microscopic findings (protein content, leukocyte numbers) were observed. The occurrence of U. urealyticum in midstream urine samples, even when numbers are considered, may be no more than a guide to the presence of ureaplasmas in the urinary tract.

Frequency of Mycoplasma hominis and Ureaplasma urealyticum infections in women with systemic lupus erythematosus

Machado,Alcyone A.; Zorzi,Alessandro R.; Gléria,Ana Eugênia A.; Donadi,Eduardo A.
Fonte: Sociedade Brasileira de Medicina Tropical - SBMT Publicador: Sociedade Brasileira de Medicina Tropical - SBMT
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2001 Português
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67.2%
Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH) have been detected in the urine of women with systemic lupus erythematosus (SLE). We evaluated the presence of these mycoplasma in the endocervix of women presenting SLE. A total of 40 SLE patients (mean age 40.2 years), and 51 healthy women (mean age 30.9 years), were studied. Endocervical swabs were cultured in specific liquid media for MH or UU, detected by a quantitative color assay, and considered positive at >10³ dilutions. Statistical analysis was performed using the two-tailed Fisher test. UU was detected in 52.5 % of patients and in 11.8% of controls (p= 0.000059). MH was detected in 20% of patients and 2% controls (p=0.003905). Both mycoplasmas were detected in 7.3% patients and 0% controls (p<0.000001). The results reported here corroborate the association of the mycoplasma infection and SLE. Thus, these agents may stimulate the production of autoreactive clones.

Freqüência de infecção pelo Mycoplasma hominis e Ureaplasma urealyticum em mulheres inférteis e relação com repercussões clínicas

Penna,Ivan Araujo; Duarte,Geraldo; Ferriani,Rui Alberto; Machado,Alcyone Artioli; Romão,Gustavo Salata; Reis,Rosana Maria dos; Moura,Marcos Dias de
Fonte: Federação Brasileira das Sociedades de Ginecologia e Obstetrícia Publicador: Federação Brasileira das Sociedades de Ginecologia e Obstetrícia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2005 Português
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67.56%
OBJETIVOS: determinar a freqüência de infecção pelo Mycoplasma hominis e Ureaplasma urealyticum e relacioná-la a variáveis clínicas de mulheres inférteis. MÉTODOS: estudo transversal com 322 pacientes inférteis submetidas à coleta de swab endocervical para pesquisa de Mycoplasma hominis e Ureaplasma urealyticum, de outubro de 2002 a maio de 2004. Todas as pacientes foram submetidas a protocolo básico de investigação clínica e laboratorial da infertilidade. Como controle, utilizou-se série histórica de 51 mulheres não gestantes, previamente pesquisadas quanto aos agentes infecciosos estudados. RESULTADOS: a freqüência de infecção pelo Mycoplasma hominis e Ureaplasma urealyticum foi de 4,9% nas pacientes inférteis e 13,8% no grupo controle. Entre as pacientes inférteis observou-se relação entre a presença dos dois patógenos e alterações no resultado da histerossalpingografia (OR: 3,20; IC 95%: 1,05-9,73), presença de dispareunia (OR: 10,72; IC 95%: 3,21-35,77) e corrimento vaginal (OR: 8,5; IC 95%: 2,83-26,02), além de cultura endocervical positiva para Escherichia coli (OR: 16,09; IC 95%: 4,95-52,25). CONCLUSÃO: a taxa de infecção pelo Mycoplasma hominis e Ureaplasma urealyticum é baixa em pacientes inférteis e está associada a seqüelas reprodutivas tardias.

Clastogenic effects of different Ureaplasma urealyticum serovars on human chromosomes

Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/1997 Português
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The possibility that Ureaplasma urealyticum might play an important role in human infertility was first raised more than 20 years ago, but this association remains speculative. Considering the hypothesis that the pathogenicity of Ureaplasma urealyticum may depend on its serotypes, the clastogenic effects of different strains of Ureaplasma urealyticum, at concentrations of 103 CCU (color changing units)/ml, 104 CCU/ml and 105 CCU/ml, were evaluated in vitro in short-term cultures of human lymphocytes. Total or partial mitotic inhibition was produced by Ureaplasma urealyticum serotypes 2, 3 and 10 independent of the concentration (103 CCU/ml, 104 CCU/ml or 105 CCU/ml) of the microorganisms employed. In contrast, the clastogenic effects observed with serotypes 1, 7 and 12 varied according to the concentration employed in the test. Mitotic alterations were observed in Ureaplasma urealyticum serotypes 5, 6, 7, 8, 9, 11 and 12. Chromatid gaps (53.0%) and chromatid breaks (13.9%) were the most frequent types of alterations observed. The results of this in vitro assay demonstrated that the clastogenic effects varied with the Ureaplasma urealyticum serotypes evaluated

Antibiotic susceptibility profiles of Mycoplasma hominis and Ureaplasma urealyticum isolated during a population-based study concerning women infertility in northeast Romania

Mihai,Mareş; Valentin,Năstasă; Bogdan,Doroftei; Carmen,Chifiriuc Mariana; Coralia,Bleotu; Demetra,Socolov
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2011 Português
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67.2%
The study was carried out on 1068 infertile women under initial evaluation. For Mycoplasma hominis, the highest resistance rates were registered for ciprofloxacin (72.22%), followed by macrolides and ofloxacin. For Ureaplasma urealyticum, the ciprofloxacin resistance was also high (51.72%), while the resistance rates to other tested antibiotics were significantly lower.

Species Identification and Subtyping of Ureaplasma parvum and Ureaplasma urealyticum Using PCR-Based Assays

Kong, Fanrong; Ma, Zhenfang; James, Gregory; Gordon, Susanna; Gilbert, Gwendolyn L.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /03/2000 Português
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There is good evidence that the organism currently known as Ureaplasma urealyticum should be divided into two species—U. parvum (previously U. urealyticum biovar 1) and U. urealyticum (previously U. urealyticum biovar 2). In this study, we designed a series of primers, targeting the 16S rRNA gene and 16S rRNA-23S rRNA intergenic spacer regions, the urease gene subunits, and the 5′ ends of the multiple-banded antigen (MBA) genes, to identify and subtype these Ureaplasma species. All of the species-specific primer pairs could distinguish the two species, but only subtype-specific primer pairs targeting the MBA genes could distinguish subtypes within each species. U. parvum was separated into three subtypes, represented by serovars 1, 3/14, and 6. U. urealyticum was also separated into three subtypes by PCR and/or direct sequencing. Subtype 1 consisted of serovars 2, 5, 8, and 9; subtype 2 contained serovars 4, 10, 12, and 13; and subtype 3 contained serovars 7 and 11. A selection of primer pairs was used to identify and subtype 78 clinical ureaplasma isolates from vaginal swabs of pregnant women and to identify and subtype ureaplasmas directly in 185 vaginal swabs in which they had been previously detected. U. parvum was identified in 228 (87%) of 263 isolates or specimens...

Comparison of PCR, Nested PCR, and Random Amplified Polymorphic DNA PCR for Detection and Typing of Ureaplasma urealyticum in Specimens from Pregnant Women

Knox, Christine L.; Timms, Peter
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /10/1998 Português
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A PCR assay, using three primer pairs, was developed for the detection of Ureaplasma urealyticum, parvo biovar, mba types 1, 3, and 6, in cultured clinical specimens. The primer pairs were designed by using the polymorphic base positions within a 310- to 311-bp fragment of the 5′ end and upstream control region of the mba gene. The specificity of the assay was confirmed with reference serovars 1, 3, 6, and 14 and by the amplified-fragment sizes (81 bp for mba 1, 262 bp for mba 3, and 193 bp for mba 6). A more sensitive nested PCR was also developed. This involved a first-step PCR, using the primers UMS-125 and UMA226, followed by the nested mba-type PCR described above. This nested PCR enabled the detection and typing of small numbers of U. urealyticum cells, including mixtures, directly in original clinical specimens. By using random amplified polymorphic DNA (RAPD) PCR with seven arbitrary primers, we were also able to differentiate the two biovars of U. urealyticum and to identify 13 RAPD-PCR subtypes. By applying these subtyping techniques to clinical samples collected from pregnant women, we established that (i) U. urealyticum is often a persistent colonizer of the lower genital tract from early midtrimester until the third trimester of pregnancy...

Growth inhibition of Ureaplasma urealyticum by the proton pump inhibitor lansoprazole: direct attribution to inhibition by lansoprazole of urease activity and urea-induced ATP synthesis in U. urealyticum.

Nagata, K; Takagi, E; Satoh, H; Okamura, H; Tamura, T
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /10/1995 Português
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The proton pump inhibitors (PPIs) omeprazole and lansoprazole and the acid-activated analog of lansoprazole AG-2000, which potently inhibit the urease of Helicobacter pylori (K. Nagata, H. Satoh, T. Iwahi, T. Shimoyama, and T. Tamura, Antimicrob. Agents Chemother. 37:769-774, 1993), also inhibited the urease activities of cell-free extracts as well as intact cells of Ureaplasma urealyticum. The 50% inhibitory concentrations were between 1 and 25 microM. These compounds also inhibited the ATP synthesis induced by urea in ureaplasma cells. The 50% inhibitory concentrations for ATP synthesis were close to those for urease activity, but they were lower than those of urease inhibitors, such as acetohydroxamic acid, hydroxyurea, and thiourea. In addition, one of the metabolites of lansoprazole found in human urine, M-VI, also inhibited ureaplasmal urease activity and the ATP synthesis induced by urea at almost the same concentrations as those of lansoprazole. The inhibition of PPIs against ureaplasma urease was very similar to those against H. pylori urease, suggesting that the inhibitory mechanism against these ureases was due to the blockage of the SH residues on the cysteine of the enzyme. Omeprazole, lansoprazole, AG-2000, and M-VI inhibited the growth of U. urealyticum. Since ureaplasma urease is thought to be involved in the pathogenicity of this organism in the urogenital tract...

Serological Typing of Ureaplasma urealyticum Isolates from Urethritis Patients by an Agar Growth Inhibition Method

Shepard, Maurice C.; Lunceford, Carl D.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1978 Português
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An agar growth inhibition method for serotyping Ureaplasma urealyticum is described, and the results of applying this method to serotyping 338 strains of the organism are presented. The serotyped strains consisted of cloned isolates from male patients with primary and recurrent nongonococcal urethritis (NGU), isolates from symptomatic patients with other genitourinary tract infections and disorders, and isolates from asymptomatic carriers of U. urealyticum in the genitourinary tract (controls). Among 122 male patients with NGU, serotype 4 was associated most frequently (52%) with this disease at Camp Lejeune, N.C. Seventeen percent of the isolates were type 2. The remaining isolates consisted of types 1, 3, 6, and 8 and accounted for 6 to 9% each of the serotypes isolated from the NGU group. Types 5 and 7 were not isolated. Among 91 symptomatic patients with other genitourinary tract infections and disorders, U. urealyticum type 4 also was associated most frequently (37%) with these disorders. The remaining isolates, represented by types 1, 2, 3, 6, 7, and 8, accounted for 9 to 15% each of the types isolated from this group. Type 5 was not isolated. Among 125 symptomfree carriers of U. urealyticum in the genitourinary tract, type 8 was recovered most frequently (30%)...

Ureaplasma urealyticum in the urethra of healthy men.

Viarengo, J; Hebrant, F; Piot, P
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /06/1980 Português
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47.47%
Nineteen healthy married white men had samples of first-voided urine cultured for Ureaplasma urealyticum at weekly intervals for 17 consecutive weeks. Of these, 63% harboured U urealyticum in their urine on at least one occasion; in 40% of the men with positive culture results ureaplasmas were isolated from 90% of samples. Those men whose specimens were frequently culture-positive had notably higher titres for U urealyticum than those whose specimens were less frequently positive. There were no significant correlation between the number of leucocytes in the urinary sediment and the amount of U realyticum in the corresponding urine specimens, although ureaplasma-positive urine specimens tended to contain slightly more leucocytes than ureaplasma-negative samples. Fourfold or higher rises in titre of U urealyticum in urine samples was not associated with urethral signs or symptoms. One man with a presumably primary infection developed dysuria. Sixty-five strains of U urealyticum were serotyped, with types 2 and 3 predominating; the serotype found in each patient with fairly constant.

Role of Ureaplasma urealyticum in lung disease of prematurity

Hannaford, K.; Todd, D.; Jeffery, H.; John, E.; Blyth, K.; Gilbert, G.
Fonte: BMJ Group Publicador: BMJ Group
Tipo: Artigo de Revista Científica
Publicado em /11/1999 Português
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47.42%
AIM—To examine the role of Ureaplasma urealyticum colonisation or infection in neonatal lung disease.
METHODS—Endotracheal aspirates from ventilated infants less than 28 weeks of gestation were cultured for U urealyticum and outcomes compared in infants with positive and negative cultures.
RESULTS—U urealyticum was isolated from aspirates of 39 of 143 (27%) infants. Respiratory distress syndrome (RDS) occurred significantly less often in colonised, than in non-colonised infants (p=0.002). Multivariate logistic regression analysis showed that in singleton infants, ureaplasma colonisation was the only independent (negative) predictor of RDS (OR 0.36; p=0.02). Both gestational age (OR 0.46; p=0.006) and isolation of U urealyticum (OR 3.0; p=0.05) were independent predictors of chronic lung disease (CLD), as defined by requirement for supplemental oxygen at 36 weeks of gestational age. Multiple gestation was also a major independent predictor of RDS and CLD.
CONCLUSIONS—Colonisation or infection with ureaplasma apparently protects premature infants against the development of RDS (suggesting intrauterine infection). However, in singleton infants, it predisposes to development of CLD, independently of gestational age. Treatment of affected infants after birth is unlikely to significantly improve the outcome and methods are required to identify and treat the women with intrauterine ureaplasmal infection...

Occurrence of Ureaplasma parvum and Ureaplasma urealyticum in Women with Cervical Dysplasia in Katowice, Poland

Ekiel, Alicja M.; Friedek, Daniela A.; Romanik, Małgorzata K.; Jóźwiak, Jarosław; Martirosian, Gayane
Fonte: The Korean Academy of Medical Sciences Publicador: The Korean Academy of Medical Sciences
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
47.42%
The aim of this study was to evaluate the occurrence of genital mycoplasmas, especially Ureaplasma parvum and Ureaplasma urealyticum, in women with atypical squamous cells of undetermined significance (ASCUS), low grade squamous intraepithelial lesions (LSIL) and high grade squamous intraepithelial lesions (HSIL), compared to women with normal cytology living in Katowice, Poland. Two sterile swabs were used to obtain material from the posterior vaginal fornix of 143 women with squamous intraepithelial lesions and 39 healthy women: first for general bacteriology, second for detection of urogenital mycoplasmas using Mycoplasma IST2 kit. From each positive Mycoplasma IST2 culture DNA was isolated and PCR was performed for identification of U. parvum and U. urealyticum. Mycoplasma IST was positive in 34.1% cases. Urogenital mycoplasmas were demonstrated in women with HSIL significantly more often compared to women with LSIL, ASCUS, and with normal cytology. DNA of U. parvum was demonstrated in majority of Mycoplasma IST2-positive cases, U. urealyticum DNA-only in 9 (4.9%). Predominance of 3/14 serovars of U. parvum was demonstrated. U. urealyticum biovar 2 was present more often in women with squamous intraepithelial lesions.

Cervical Cytopathological Findings in Korean Women with Chlamydia trachomatis, Mycoplasma hominis, and Ureaplasma urealyticum Infections

Choi, Yuri; Roh, Jaesook
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
Publicado em 08/01/2014 Português
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47.42%
This is to investigate the cervical cytological abnormalities associated with Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum infections on routine screen. A total of 714 subjects who had undergone cervical Pap smears and concomitant analyses for cervical infections were included by a retrospective search. The frequencies of reactive cellular change (RCC) and squamous epithelial abnormalities were significantly higher in Chlamydia positive subjects than in uninfected subjects (P < 0.001). Of the 124 subjects tested for M. hominis, M. genitalium, and U. urealyticum, 14 (11%) were positive for M. hominis and 29 (23%) were positive for U. urealyticum. Squamous abnormalities were more frequent in subjects with Ureaplasma infections than in uninfected subjects (24% versus 8%). Taking together these findings, C. trachomatis and U. urealyticum may have a causal role in the development of cervical epithelial changes, including RCC. Thus, extra awareness is warranted in cervical screening of women with Chlamydia or Ureaplasma infections.

Small repeating units within the Ureaplasma urealyticum MB antigen gene encode serovar specificity and are associated with antigen size variation.

Zheng, X; Teng, L J; Watson, H L; Glass, J I; Blanchard, A; Cassell, G H
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /03/1995 Português
Relevância na Pesquisa
47.46%
Ureaplasma urealyticum is a common commensal of the female lower urogenital tract, yet it has been shown to be an important cause of chorioamnion infection, respiratory and central nervous system disease, and death in premature infants. It has been suggested that only certain serovars are capable of producing invasive disease. However, we previously showed that many serotypes are invasive and that perhaps antigen variability and host factors are more important determinants of ureaplasma infections than are different serotypes per se. The molecular characterization in this report describes a mechanism available to ureaplasmas for producing antigen variation. That antigen, designated MB and previously identified on U. urealyticum, contains serovar-specific and cross-reactive epitopes, is produced both in vitro and in vivo, is a predominant antigen recognized during ureaplasma infections of humans, undergoes a high rate of size variation in vitro, and is size variable on invasive ureaplasma isolates. In the present study, we cloned and sequenced the gene of the MB antigen from serovar 3, the serovar most commonly isolated from humans. The 3' two-thirds of the gene was shown to contain identical 18-nucleotide tandem repeats. PCR analysis and direct sequencing of two variants indicated that alterations within this repeat region are responsible for the size variation of the MB antigen. Intact recombinant serovar 3 MB antigen and truncated products...

Estudio de Chlamydia trachomatis, Ureaplasma urealyticum y Mycoplasma hominis en pacientes infértiles y abortadoras habituales

Ortiz Rodríguez E,Cecilia E.; Hechavarría Calderín,Clara E.; Ley Ng,Mirta; Álvarez Medina,Georgina; Hernández Ortiz,Yosmel
Fonte: Revista Cubana de Obstetricia y Ginecología Publicador: Revista Cubana de Obstetricia y Ginecología
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2010 Português
Relevância na Pesquisa
47.6%
Algunos microorganismos como Chlamydia trachomatis, Ureaplasma urealyticum y Mycoplasma hominis han sido relacionados con diferentes procesos patológicos en mujeres sexualmente activas. OBJETIVO: Para conocer la frecuencia de estos agentes se realizó un estudio descriptivo observacional en el Hospital Ginecoobstétrico Ramón González Coro, en el período de noviembre de 2008 a enero de 2009. MÉTODOS: Se estudiaron 166 muestras de exudados vaginales y endocervicales tomadas a pacientes que acudieron a las consulta de infertilidad y abortadoras habituales. Se aplicaron los test diagnósticos para Chlamydia y micoplasmas genitales. RESULTADOS: De las pacientes estudiadas, 113 fueron positivas (68 %), el test de Chlamydia se aplicó y 75 resultaron positivas a este microorganismo (45,2 %), mientras que 57 fueron positivas a micoplasmas genitales (34,3 %), de ellas 50 a Ureaplasma urealyticum, 4 a Mycoplasma hominis y en 3 muestras se aislaron Ureaplasma urealyticum más Mycoplasma hominis. Se encontró asociación de estos agentes con otros microorganismos. El 50,9 % de los casos positivos a Mycoplasma tuvieron una infección moderada (29/57). El test de Mycoplasma permitió conocer la susceptibilidad de estos agentes frente a diferentes antimicrobianos; se encontró para Ureaplasma urealyticum una resistencia de 24 % para la ofloxacina; 22 % para la tetraciclina y 20 % para la claritromicina; por su parte Mycoplasma hominis mostró el 75 % de resistencia a la eritromicina y 100 % a claritromicina y azitromicina; en los tres casos que coincidieron Ureaplasma urealyticum y Mycoplasma hominis se observó una resistencia elevada a la azitromicina...

Artritis reactiva a Ureaplasma urealyticum: Ureaplasma Urealyticum-induced arthritis

Lizagarra,Mariana; Fernández,Alejandro; Consani,Sandra; Maciel,Gabriel; Larre Borges,Alba
Fonte: Archivos de Medicina Interna Publicador: Archivos de Medicina Interna
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2010 Português
Relevância na Pesquisa
67.37%
Las artritis reactivas pertenecen al grupo de las espondiloartropatías, constituyen una complicación alejada inmunomediada de infecciones frecuentes, con manifestaciones articulares y sistémicas floridas, de evolución benigna en general, si bien puede tener un curso crónico y agresivo. Se describe un caso de artritis reactiva a Ureaplasma urealyticum, se actualizan aspectos teóricos del tema y sus aspectos más controversiales

Frequency and antimicrobial sensitivity of Ureaplasma urealyticum and Mycoplasma hominis in patients with vaginal discharge

Díaz,Leonor; Cabrera,Luis E.; Fernández,Tania; Ibáñez,Inailay; Torres,Yulian; Obregón,Yakelín; Rivero,Yanelys
Fonte: Medical Education Cooperation with Cuba Publicador: Medical Education Cooperation with Cuba
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2013 Português
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67.2%
Determination of antimicrobial sensitivity helps establish adequate treatment and avoids future genital tract diseases in women of fertile age. In Cuba, prevalence of mycoplasma in patients with vaginal discharge is unknown. The objective of this research was to determine frequency and antimicrobial sensitivity of Ureaplasma urealyticum and Mycoplasma hominis in women with vaginal discharge through analysis of laboratory data from vaginal smears from 255 patients referred to the Municipal Hygiene and Epidemiology Center in Güines, Mayabeque Province, Cuba. Mycoplasma System Plus (Italy) was used for detection, identification, count and sensitivity testing. The finding of mycoplasmas in almost two thirds of specimens examined suggests that the sexually active female population should be screened for these bacteria and that barrier contraception methods should be promoted to decrease their spread and prevent longterm sequelae. Such updating of local patterns of antimicrobial resistance supports decision making for best treatment options in patients with these infections. Our results should help clinicians in our area choose an antibiotic, and also confirm the utility of Mycoplasma System Plus for mycoplasma research in resource-scarce settings...