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Effects of gonadotropin-releasing hormone at initiation of the 5-d timed artificial insemination (AI) program and timing of induction of ovulation relative to AI on ovarian dynamics and fertility of dairy heifers

LIMA, F. S.; AYRES, H.; FAVORETO, M. G.; BISINOTTO, R. S.; GRECO, L. F.; RIBEIRO, E. S.; BARUSELLI, P. S.; RISCO, C. A.; THATCHER, W. W.; SANTOS, J. E. P.
Fonte: ELSEVIER SCIENCE INC Publicador: ELSEVIER SCIENCE INC
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
66.68%
Two experiments evaluated the effects of the first GnRH injection of the 5-d timed artificial insemination (AI) program on ovarian responses and pregnancy per AT (P/AI), and the effect of timing of the final GnRH to induce ovulation relative to AT on P/AI. In experiment 1, 605 Holstein heifers were synchronized for their second insemination and assigned randomly to receive GnRH on study d 0 (n = 298) or to remain as untreated controls (n = 307). Ovaries were scanned on study d 0 and 5. All heifers received a controlled internal drug-release (CIDR) insert containing progesterone on d 0, a single injection of PGF(2 alpha),, and removal of the CIDR on d 5, and GnRH concurrent with timed AT on d 8. Blood was analyzed for progesterone at AI. Pregnancy was diagnosed on d 32 and 60 after AI. Ovulation on study d 0 was greater for GnRH than control (35.4 vs. 10.6%). Presence of a new corpus luteum (CL) at PGF(2 alpha),, injection was greater for GnRH than for control (43.1 vs. 20.8%), although the proportion of heifers with a CL at PGF(2 alpha) did not differ between treatments and averaged 87.1%. Progesterone on the day of AT was greater for GaRH than control (0.50 +/- 0.07 vs. 0.28 +/- 0.07 ng/mL). The proportion of heifers at AI with progesterone <0.5 ng/mL was less for GURH than for control (73.8 vs. 88.2%). The proportion of heifers in estrus at AI did not differ between treatments and averaged 66.8%. Pregnancy per AI was not affected by treatment at d 32 or 60 (GnRH = 52.5 and 49.8% vs. control = 54.1 and 50.0%)...

Effect of nAellore cows ciclicity on conception and pregnant rates after synchronization protocols for fixed timed artificial insemination

Palhano, Helcimar Barbosa; Teixeira de Jesus, Vera Lucia; Abidu-Figueiredo, Marcelo; Baldrighi, Julia Maria; Burg de Mello, Marco Roberto
Fonte: SOC MEDICINA VETERINARIA ESTADO RIO DE JANEIRO; RIO DE JANEIRO Publicador: SOC MEDICINA VETERINARIA ESTADO RIO DE JANEIRO; RIO DE JANEIRO
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
66.67%
Palhano H.B., Jesus V.L.T., Abidu-Figueiredo M., Baldrighi J.M. & Mello M.R.B. [Effect of nAellore cows ciclicity on conception and pregnant rates after synchronization protocols for fixed timed artificial insemination]. Efeito da ciclicidade de vacas Nelore sobre as taxas de concepcao e de prenhez apos protocolos de sincronizacao para inseminacao artificial em tempo fixo. Revista Brasileira de Medicina Veterinaria, 34(1):63-68, 2012. Departamento de Biologia Animal, Universidade Federal Rural do Rio de Janeiro, BR 465 km 7, Seropedica, RJ 23890-000, Brasil. Email: hbpalhano@gmail.com The present study evaluated the effect on conception and pregnancy rates of Nellore cows selected for Fixed Timed Artificial Insemination (FTAI) program, submitted to four synchronization protocols. Four hundred and ninety lactating females were used and assigned to eight groups: I-OvSynch, n=68, with selection of cycling cows; II-OvSynch + progesterone (P-4), n=67, after selection of non-cycling animals; III-OvSynch, without selection, n=68; IV-OvSynch + P-4, without selection, n=67; V-Co-Synch, n=55, with selection of cycling cows; VI-Co-Synch + P-4, n=55, with selection non-cycling cows; VII- Co-Synch without selection, n=55; VIII- Co-Synch + P-4, without selection...

Parâmetros reprodutivos de perdizes machos (Rhynchotus rufescens) criadas em cativeiro: comparação entre os índices reprodutivos de animais acasalados e inseminados; Reproductive parameters of captive male partridge (Rhynchotus rufescens): artificial insemination versus natural service

Cavalcante, Ana Karina da Silva
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 24/04/2006 Português
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A perdiz (Rhynchotus rufescens) é um tinamídeo com amplos músculos peitorais muito apreciados por mercados especializados. No entanto, a produção em larga escala é inexpressiva, podendo ser melhorada através da inseminação artificial (IA). Sendo assim, o presente experimento teve como objetivos padronizar a coleta de sêmen, o teste hipo-osmótico, testar diferentes diluidores, tempos de refrigeração e doses inseminantes. Amostras seminais de 100 animais, pertencentes a FCAV/UNESP/Jaboticabal, foram empregadas na descrição do volume, pH, motilidade, vigor, concentração e alterações morfológicas. As coletas de sêmen foram realizadas em 2 ciclos. No primeiro ciclo foram realizadas a padronização da técnica de coleta de sêmen e a determinação do melhor meio hipo-osmótico, por meio de oito soluções. No segundo ciclo de coletas aplicou-se as técnicas já padronizadas; descreveu-se a motilidade e vigor espermático de amostras seminais diluídas em meios para sêmen de peru, TCM 199, TQC e solução fisiológica, refrigeradas por até 48h; além de terem sido realizadas IA em 128 fêmeas utilizando sêmen de 32 machos, os animais foram agrupados em quintetos (1 macho:4 fêmeas) no início do ciclo reprodutivo de 2004-2005. Metade dos quintetos foi mantida no sistema de monta natural e empregada como grupo controle (GC). A outra metade foi mantida em quintetos por apenas 2 meses. Em seguida...

Situação atual da inseminação artificial em suínos; Present situation of artificial insemination in swine

Bortolozzo, Fernando Pandolfo; Wentz, Ivo; Dallanora, Djane
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
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66.69%
A inseminação artificial na espécie suína trouxe benefícios pela difusão rápida de características desejáveis no rebanho como a melhora de ganho de peso e conversão alimentar, menor deposição de gordura e melhor qualidade de carcaça, melhor aproveitamento de machos geneticamente superiores e redução dos custos de produção. Essas características permitiram a grande difusão da biotécnica na suinocultura intensiva e tecnificada. Nos últimos 30 anos, poucas modificações foram feitas na realização da diluição e técnica de inseminação propriamente dita. Porém, existiram progressos no desenvolvimento de novos equipamentos para conservação do sêmen e utilização de materiais descartáveis na produção das doses. Atualmente, o foco da pesquisa está direcionado à redução no número de espermatozóides por dose, deposição do sêmen intra-uterinamente e modulação da fagocitose das células espermáticas no trato genital feminino. Esse artigo apresenta uma revisão referindo-se aos progressos já alcançados e às perspectivas de utilização de novas tecnologias na área de manejo reprodutivo em suínos, principalmente visando a de possibilidade de redução do número de espermatozóides/ fêmea/ano.; The artificial insemination has improve in pig production optimizing the use of boars...

Inseminação artificial pós-cervical em primíparas suínas; Post cervical artificial insemination in primiparous sows

Sbardella, Pedro Ernesto
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Dissertação Formato: application/pdf
Português
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Este estudo avaliou a performance reprodutiva de primíparas suínas submetidas à inseminação artificial pós-cervical (IAPC) comparada à inseminação artificial cervical (IAC). A dificuldade na introdução do cateter, ocorrência de sangue ou refluxo durante a inseminação e o volume e o total de células refluídas até 60 minutos após a inseminação também foram avaliados. As fêmeas foram homogeneamente distribuídas, de acordo com a perda de peso na lactação, duração da lactação, número de leitões desmamados, intervalo desmame-estro e nascidos totais no parto anterior, em dois tratamentos: IAPC (n=165) com 1,5 x 109 células espermáticas em 45 ml e IAC (n=165) com 3 x 109 células espermáticas em 90 ml. Foi realizada ultrassonografia transabdominal em tempo real no momento em que as fêmeas apresentaram estro e 24 horas após a última inseminação. Não houve diferença entre os tratamentos na taxa de parto e no tamanho da leitegada (P > 0,05). O sucesso na passagem do cateter intra-uterino em todas as inseminações foi possível em 86,8% (165/190) das fêmeas inicialmente selecionadas para o tratamento IAPC. A dificuldade na introdução do cateter em pelo menos uma inseminação não afetou a performance reprodutiva das fêmeas do tratamento IAPC (P > 0...

Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows II: Strategies and factors affecting fertility

Sa Filho, O. G.; Meneghetti, M.; Peres, R. F. G.; Lamb, G. C.; Vasconcelos, J. L. M.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 210-218
Português
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In Experiments 1, 2, and 3, we evaluated the effects of temporary weaning (TW), equine chorionic gonadotropin (eCG), and follicle-stimulating hormone (FSH) treatments on results of it fixed-time artificial insemination (TAI) protocol in postpartum Bos indicus cows. In Experiment 1, treatment with 400 IU eCG or with TW for 48 h consistently improved pregnancy rates (PRs) at TAI, but, in Experiment 2, FSH treatment was less effective than eCG or TW. In Experiment 3, the inclusion of eCG treatment in cows subjected to TW did not improve PRs. We concluded that TW or 400 IU eCG should be included in the TAI protocol in postpartum Bos indicus cows to enhance fertility. In Experiment 4, we used records front heifers and cows treated with the proposed protocol during the 2006-2007 (n = 27,195) and 2007-2008 (n = 36,838) breeding seasons from multiple locations in Brazil to evaluate factors potentially affecting PRs. Overall PR at TAI was 49.6% (31,786 of 64,033). Pregnancy rate differed (P < 0.01) among farm within location (results ranging between 26.8% and 68.0%; P < 0.01), cow group within farm, by breed (Bos indicus, 48.3% [26,123 of 54,145]; Bos taurus, 61.7% [3652 of 5922]; and crossbred Bos indicus x Bos taurus, 50.7% [2011 of 3966])...

Comparison of efficiency between two artificial insemination methods using frozen-thawed semen in domestic cat (Felis catus) Artificial insemination in domestic cats

Silva Balbin Villaverde, Ana Izabel; Melo, Cely Marini; Martin, Ian; Ferreira, Tatiana Henriques; Papa, Frederico Ozanam; Taconeli, Cesar Augusto; Lopes, Maria Denise
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 434-442
Português
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); The aim of this study was to compare the efficiency of the intravaginal (IVAI) vs. intrauterine artificial insemination (IUAI) using frozen-thawed sperm in the domestic cat. Semen was collected from two tom cats using an artificial vagina and samples were assessed for motility (computer-assisted sperm analysis (CASA)), sperm morphology and plasma membrane integrity. After dilution with TRIS/OEP/YOLK (4% of glycerol), sperm samples were loaded into 0.25 mL straws (25 x 106 motile sperm/straw), incubated at 5 degrees C for 20 min and cryopreserved over liquid nitrogen (LNZ) vapor for 15 min and then immersed in LN(2). For each AI, four straws from the same male were thawed (12 s at 46 degrees C) and centrifuged at 250 x g for 8 min to pellet the sperm. The supernatant was discarded and sperm pellet resuspended with the remaining liquid, approximately 100 mu L, and analyzed as described above. Queens were treated with a single im injection of 100 IU eCG to induce ovarian follicular development. Final oocyte maturation and ovulation was induced with 100 IU hCG given im at 82-84 h after eCG administration. Thirty hours after hCG administration, females were inseminated either intrauterine (n=8 queens) or intravaginally (n=8 queens)...

Neither plasma progesterone concentrations nor exogenous eCG affects rates of ovulation or pregnancy in fixed-time artificial insemination (FTAI) protocols for puberal Nellore heifers

Pegorer, M. Figueira; Ereno, R. L.; Satrapa, R. A.; Pinheiro, V. G.; Trinca, L. A.; Barros, C. M.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 17-23
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The objective was to evaluate the effects of plasma progesterone (P4) concentrations and exogenous eCG on ovulation and pregnancy rates of pubertal Nellore heifers in fixed-time artificial insemination (FTAI) protocols. In Experiment 1 (Exp. 1), on Day 0 (7 d after ovulation), heifers (n = 15) were given 2 mg of estradiol benzoate (EB) im and randomly allocated to receive: an intravaginal progesterone-releasing device containing 0.558 g of P4 (group 0.5G, n = 4); an intravaginal device containing 1 g of P4 (group 1G, n = 4); 0.558 g of P4 and PGF2α (PGF; 150 μg d-cloprostenol, group 0.5G/PGF, n = 4); or 1 g of P4 and PGF (group 1G/PGF, n = 3). On Day 8, PGF was given to all heifers and intravaginal devices removed; 24 h later (Day 9), all heifers were given 1 mg EB im. In Exp. 2, pubertal Nellore heifers (n = 292) were treated as in Exp. 1, with FTAI on Day 10 (30 to 36 h after EB). In Exp. 3, pubertal heifers (n = 459) received the treatments described for groups 0.5G/PGF and 1G/PGF and were also given 300 IU of eCG im (groups 0.5G/PGF/eCG and 1G/PGF/eCG) at device removal (Day 8). In Exp. 1, plasma P4 concentrations were significantly higher in heifers that received 1.0 vs 0.588 g P4, and were significantly lower in heifers that received PGF on Day 0. In Exp. 2 and 3...

Use of cooled bull semen as a strategy to increase the pregnancy rate in fixed-time artificial insemination programs-case report

Crespilho, Andre Maciel; Papa, Frederico Ozanam; Santos, Marcos de Paula; Sá Filho, Manoel Francisco de
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 175-179
Português
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Semen cryopreservation is still considered suboptimal due to lower fertility when compared to fresh semen. The reasons for the loss of fertility are various and related to irreversible damage caused to the cells during the freeze-thaw process. An alternative to conventional cryopreservation represents the use of chilled bull semen, preventing the damage associated with freezing, thereby guaranteeing greater sperm viability. The aim of this study was to describe the use of cooled bull semen as a strategy to increase the pregnancy for Fixed-Time Artificial Insemination (FTAI) of Nellore (Bos indicus) cows. One ejaculate of a select Nellore bull obtained by electroejaculation was used; the semen sample was fractioned into two aliquots: one diluted in Botu-Bov® extender containing 6.4% glycerol for cryopreservation (BB-F, frozen group) and one diluted in the same extender, free from cryoprotectants and used for cooling (BB-C, cooled semen group). The samples in the BB-C group were chilled to 5°C using an isothermic box and maintained for 24 h prior to use. A total of 349 lactating Nellore cows (70-90 days after birth) were synchronized by the insertion of a progesterone releasing device (1.0 g) and estradiol benzoate (2.0 mg i.m.) on a random day of the estrous cycle (Day 0); FTAI was performed 44-48 h after the removal of the device. The pregnancy rates were 45.71 and 61.49% (P<0.05)...

Comparison between deep intracornual artificial insemination (dIAI) and conventional artificial insemination (AI) using low concentration of spermatozoa in beef cattle

Meirelles,Ciro; Kozicki,Luiz Ernandes; Weiss,Romildo Romualdo; Segui,Marcio Saporski; Souza,Antonio; Santos,Ivo Walter dos; Breda,Jose Carlos dos Santos
Fonte: Instituto de Tecnologia do Paraná - Tecpar Publicador: Instituto de Tecnologia do Paraná - Tecpar
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2012 Português
Relevância na Pesquisa
66.82%
This study aimed to compare the pregnancy rate using the conventional artificial insemination (AI) or deep intracornual artificial insemination (DIAI), with low number of spermatozoa (4.0 million sperm) in 270 Nelore cows. The animals were divided in two groups (G: G1 (135 cows) conventional AI was performed (=semen deposition in the uterine body) and in G2 (135 cows) to DIAI, in ipsilateral horn where the dominant follicle in the ovary had previously been detected, by ultrasound examinations. For both the methods, a single artificial insemination was carried out after visual estrus observation, checked three times a day (morning, afternoon and evening). The pregnancy diagnosis after 45 days was conducted by ultrasound. Results showed a better pregnancy rate in the DIAI group (67.4% - p<0.01), when compared to conventional AI (48.8%) with low spermatozoa concentration.

Artificial insemination in goats.

NUNES, J. F.
Fonte: In: INTERNATIONAL CONFERENCE ON GOATS, 4., 1987, Brasília, DF. Proceedings... Brasília, DF: EMBRAPA-DDT, 1987. v. 1. p. 733-743. Publicador: In: INTERNATIONAL CONFERENCE ON GOATS, 4., 1987, Brasília, DF. Proceedings... Brasília, DF: EMBRAPA-DDT, 1987. v. 1. p. 733-743.
Tipo: Artigo em anais de congresso (ALICE)
Português
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Technical and economical evaluation of determined practices of reproduction and breeding, focused here on artificial insemination of reproduction and breeding, focused here on artificial insemination and the further measurement of matings, shows a high productive efficiency in the development of dairy goats. Artificial insemination in Brazil, especially in the Northeast, spread in a rapid, massive and positive way to determine genetic characteristics for dairy production. This program through the utilization of tested bucks has contributed to the firs offspring with significant increases in milk production. The presentation of the best breeds through a germplasm bank, using improvement technology of goat semen has made possible new technological alternatives for goat breeders program of dairy goats in the Northeast and for extension to other parts of Brazil.; 1987

Intrauterine artificial insemination of swine with different sperm concentrations, parities,and methods for prediction of ovulation

Serret, Carolina Gon??alves; Alvarenga, Marcus Vinicius Figueira de; C??ria, A.L.P.; Dias, C.P.; C.P., C.D.; Corr??a, Marcio Nunes; Deschamps, Jo??o Carlos; Bianchi, Ivan; Lucia Junior, Thomaz
Fonte: Universidade Federal de Pelotas Publicador: Universidade Federal de Pelotas
Tipo: Artigo de Revista Científica
Português
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This study compared the reproductive performance of sows that were submitted to cervical artificial insemination (CAI) using concentrations of 3.5 x 109 spermatozoa per dose or intrauterine artificial insemination (IUAI) with 2, 1, or 0.5 x 109 spermatozoa per dose. Within all treatments, females were inseminated either after conventional estrus detection or after ultrasound-guided ovulation diagnosis. Farrowing rate did not differ between CAI and IUAI, regardless of the concentration of spermatozoa used (P > 0.05). In comparison with CAI in parity-3+ females, the probability of failure to farrow was greater (P < 0.05) with IUAI in parity-1 females inseminated with 2 x 109 spermatozoa per dose, parity-2 females with any concentration, and parity-3+ females inseminated with 0.5 x 109 spermatozoa per dose (P < 0.01). Total litter size did not differ across AI methods (P > 0.05), but it was smaller (P < 0.0005) in parity-1 females than for higher parity females. Total litter size was lower with IUAI in parity-1 females with any concentration and in parity-2 females with 2.0 x 109 spermatozoa per dose (P < 0.05). The estimation of ovulation via conventional estrus detection or ultrasound, as well as the occurrence of semen backflow and bleeding during AI...

Efici??ncia reprodutiva com insemina????o artificial intra-cervical e p??s-cervical, em f??meas su??nas, associada ?? concentra????o esperm??tica e perfil estral; Reproductive efficiency with intra-cervical and post-cervical artificial insemination in female swine associated with spermatozoa concentration and estrus profile

SERRET, Carolina Gon??alves
Fonte: Universidade Federal de Pelotas; Biotecnologia; Programa de P??s-Gradua????o em Biotecnologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Biotecnologia; Programa de P??s-Gradua????o em Biotecnologia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
Português
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Artificial insemination (AI) is a technique intensively used in the swine farms nowadays, due to its benefits related to genetic improvement and health status, allowing the industry to market high-quality healthy products. The post-cervical AI (PCAI) allows the deposition of semen in the uterine body, using 1 x 10 9 spermatozoa dose. Thus, with PCAI the fertility of one particular boar can influence the reproductive performance of a much higher number of females than in ICAI-based systems. The objective of this study was to compare the subsequent reproductive performance of swine breeding females submitted to either intra-cervical artificial insemination (ICAI) with sperm concentrations of 3.5 x 10 9 spermatozoa per dose or post-cervical artificial insemination (PCAI) with 2, 1, or 0.5 x 10 9 spermatozoa per dose. The study was conducted in a commercial farm including 338 F1 females. Among them, 232 were first inseminated 12 h after estrus detection, receiving the subsequent doses after 12 h intervals, whereas 106 were inseminated right after ovulation diagnosis through real-time ultrasound. Conception and farrowing (FR) rates for ICAI (98.9% e 97.9%, respectively) did not differ (P < 0,001) from those observed for PCAI (87.1% e 84.3%...

Efeito da insemina????o artificial transcervical e da aplica????o de flunixin meglumine sobre a taxa de prenhez de ovelhas; Effect of transcervical artificial insemination and of the flunixin meglumine application on the pregnancy rate of ewes

RABASSA, Viviane Rohrig
Fonte: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR Publicador: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR
Tipo: Dissertação Formato: application/pdf
Português
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The artificial insemination (AI) with frozen semen in sheep is limited by the high costs of the laparoscopic technique and the variability of the results obtained from transcervical technique. This technique requires attachment of cervix, a procedure that can cause located injuries, further, the extensive uterine manipulation, which can lead to an exacerbated endometrial prostaglandin synthesis, could modify the uterine environment at the moment of conception. The objective of this study was to compare the efficiency of transcervical AI, through the technique of cervix attachment and retraction, in comparison to laparoscopic AI, as well as, to determine the effect of flunixin meglumine application at the moment of AI, on pregnancy rate of ewes, using frozen semen. In fixed-time inseminated ewes, during seasonal anestrous, there was no difference in the pregnancy rate between the transcervical and laparoscopic techniques, which was 40%. In respect to flunixin meglumine, the experiment was carried out during the breeding season and was performed using AI with estrus detection. There was no difference in the pregnancy rate between ewes receiving or not flunixin meglumine (p>0.05), which was 68.9% for the control group and 60.0% for the group that received flunixin meglumine at the moment of transcervical AI. These results were probably due to the minimum manipulation exerted on the cervix of these females...

Artificial insemination in marsupials

Rodger, J.; Paris, D.; Czarny, N.; Harris, M.; Molinia, F.; Taggart, D.; Allen, C.; Johnston, S.
Fonte: Elsevier Science Inc Publicador: Elsevier Science Inc
Tipo: Artigo de Revista Científica
Publicado em //2009 Português
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Assisted breeding technology (ART), including artificial insemination (AI), has the potential to advance the conservation and welfare of marsupials. Many of the challenges facing AI and ART for marsupials are shared with other wild species. However, the marsupial mode of reproduction and development also poses unique challenges and opportunities. For the vast majority of marsupials, there is a dearth of knowledge regarding basic reproductive biology to guide an AI strategy. For threatened or endangered species, only the most basic reproductive information is available in most cases, if at all. Artificial insemination has been used to produce viable young in two marsupial species, the koala and tammar wallaby. However, in these species the timing of ovulation can be predicted with considerably more confidence than in any other marsupial. In a limited number of other marsupials, such precise timing of ovulation has only been achieved using hormonal treatment leading to conception but not live young. A unique marsupial ART strategy which has been shown to have promise is cross-fostering; the transfer of pouch young of a threatened species to the pouches of foster mothers of a common related species as a means to increase productivity. For the foreseeable future...

The influence of insemination dose on pregnancy per fixed-time artificial insemination in beef cows is affected by semen extender

Crespilho, A. M.; Papa, Frederico Ozanam; Sa Filho, M. F.; Guasti, P. N.; Dell'aqua Junior, José Antônio; Vasconcelos, José Luiz Moraes; Novaes, J. L. C.; Martins, A.
Fonte: Brazilian Coll Animal Reproduction Publicador: Brazilian Coll Animal Reproduction
Tipo: Artigo de Revista Científica Formato: 3-10
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 04/12962-2; The aim of this study was to compare the efficacy of two extenders (Tris-egg yolk - TRIS and Botu-Bov (R) - BB, Botupharma, Brazil) for bovine semen cryopreservation with a different number of sperm per straw (6, 12, 25 or 50 x 10(6) sperm per straw) on post-thaw seminal viability (experiment 1) and pregnancy per artificial insemination (P/AI; experiment 2). In experiment 1, higher values of linearity and straightness associated to low amplitude of lateral head displacement (ALH) were observed in samples cryopreserved with BB extender, when compared to samples cryopreserved with TRIS extender (P < 0.05) regardless of the sperm concentration per straw. The pregnancy rates were 57.63, 60.32, 59.26 and 62.50% respectively for 6, 12, 25 or 50 x 10(6) sperm/straw in BB samples and 45.61, 48.84, 60.34 and 70.59% respectively for the TRIS extender. Increasing the number of sperm had a significant effect on P/AI (P < 0.05) when TRIS extender was used. In conclusion, Botu-Bov (R) extender promotes better post-thaw sperm movement. The increase in the number of sperm cells per insemination dose improved P/AI rates in Bos indicus lactating beef cows inseminated when using Tris-egg yolk extender.

Única ou dupla inseminação artificial em tempo fixo em porcas com ovulações induzidas pelo Hormônio Luteinizante; Single or double artificial insemination in fixed time in sows with ovulation induced by Luteinizing Hormon

Candini, Paulo Henrique; Moretti, Aníbal de Sant'Anna; Zanella, Eraldo Luis; Silveira, Paulo Roberto Souza da; Viana, Carlos Henrique Cabral; Santos, Isabel
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; ; Formato: application/pdf
Publicado em 01/04/2004 Português
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Duzentas e cinqüenta e quatro matrizes Camborough 22 (PIC®), foram divididas em 3 tratamentos: T 1 (n=60) - 600 UI de eCG após desmama e 5 mg de LH, 72 h após eCG , com única inseminação artificial (IA) (24 h após LH); T 2 (n=95) - mesmo tratamento hormonal do T1, com 2 IA (24 e 32 h após LH); T 3 (n=99) - grupo controle sem tratamento hormonal, com 3 IA. As médias de intervalo desmame-estro (IDE) em T1, T2 e T3 foram de 87,4 ± 3,0 (87 a 111), 87 ± 0 (87) e 99,9 ± 13,6 (63 a 135) horas, respectivamente, sendo reduzidas (P < 0,0001) pelas gonadotrofinas. A duração do estro (DE) foi de 44,3 ± 8,78 (12 a 60), 41,3 ± 9,77 (24 a 60) e 60,1 ± 10,22 (36 a 84) ;horas;, respectivamente para T1, T2 e T3, sendo menor (P < 0,0001) nas fêmeas tratadas. As diferenças no intervalo LH e ovulação (LH-OV) entre o grupo controle (56,1 ± 15,91, variação de 21 a 93 horas) e os grupos tratados (35,7 ± 6,07 em T1 e 35,5 ± 6,06 em T2, com variação de 30 a 42 horas) foram significativas (P < 0,0001). O tamanho de leitegada (TL) foi de 10,6 ± 3,25 (2 a 16) em T1, 11,3 ± 3,0 (4 a 20) em T2 e 11,6 ± 2,74 (4 a 18) leitões em T3, enquanto os mesmos demonstraram número de leitões nascidos vivos (NV) de 9,6 ± 3,14 (2 a 16)...

Inseminação artificial em cabras com sêmen congelado: resultados preliminares; Artificial insemination in goats with frozen semen: preliminary results

Ferrari, Silvia; Leinz, Frederico; Barnabe, Valquíria Hyppolito
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/01/1998 Português
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Com o objetivo de avaliar o sêmen de caprinos congelado em diluidor à base de TRIS contendo baixa quantidade de gema de ovo, foi feita a análise da motilidade retilínea e progressiva logo após o congelamento e após 4 anos de estocagem em nitrogênio líquido, resultando respectivamente em 38,2 ± 7,47% e 31,43 ± 13,55%. Foi feita a sincronização do cio de 16 cabras com esponjas intravaginais impregnadas com progesterona e aplicações de PMSG no nono dia. No 11º dia as esponjas foram retiradas e após 46 horas foi feita a inseminação artificial com palhetas estocadas por 4 anos. Houve 31,2% de não-retorno ao cio. O diluidor à base de TRIS contendo baixa quantidade de gema de ovo se mostrou eficiente quanto à preservação dos espermatozóides quando avaliados in vitro através da motilidade retilínea e progressiva e in vivo através da inseminação artificial.; Aiming to evaluate frozen goat semen in TRIS diluent with low egg yolk addition, progressive motility was evaluated soon after freezing and again 4 years later, resulting in 38.2 ± 7.4% and 31.4 ± 13.5%, respectively. Sixteen goats were oestrus synchronized through progesterone impregnated intravaginal sponges followed by PMSG injection on day 9. Vaginal sponges were withdrawn on day 11...

Comparação dos índices reprodutivos com inseminação artificial ou cobertura natural sob influências sazonais em suínos; Comparative reproductive rates using artificial insemination and natural mating under seasonal influences in swine

Candini, Pedro Henrique; Viana, Carlos Henrique Cabral; Madureira, Ed Hoffman; Arruda, Rubens Paes de; Celeghini, Eneiva Carla Carvalho; Assumpção, Mayra Elena Ortiz D’Avila; Gusmões, Pedro Paulo Gimenes; Valentin, Renato; Visintin, José Antonio
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/12/2000 Português
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66.72%
Os resultados das taxas de fertilidade e dos tamanhos das leitegadas foram analisados na granja de suínos da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo no Campus de Pirassununga, durante o período de 4 anos (1992 a 1995), com o objetivo de comparar a inseminação artificial (IA) e a cobertura natural (CN) em diferentes épocas do ano, procurando estudar as influências sazonais sobre os índices reprodutivos. Foram avaliadas 799 coberturas em fêmeas das raças Landrace (L), Large White (LW) e mestiças, sendo 539 de IA e 260 de CN. Reprodutores (L e LW) de comprovada fertilidade foram utilizados tanto para CN quanto para IA. As doses de sêmen apresentavam concentração mínima de 3 bilhões de espermatozóides em volume de 100 ml. As IA foram realizadas às 12 e às 24 horas após reflexo positivo de tolerância ao macho, enquanto as montas foram realizadas no momento e às 24 horas após o diagnóstico do cio. Os índices de fertilidade foram de 72,9% e 75,8% e o número de leitões nascidos de 12,4 e 12,1, respectivamente, para inseminação artificial e monta natural, não mostrando diferença significativa. A estação climática influenciou a taxa de parição (71,2%, 81,4%, 76,9% e 66,4%...

REDUCTION OF THE VOLUNTARY WAITING PERIOD BY USE OF HORMONAL PROTOCOLS AND FIXED-TIME ARTIFICIAL INSEMINATION (FTAI) IN DAIRY COWS; REDUCTION OF THE VOLUNTARY WAITING PERIOD BY USE OF HORMONAL PROTOCOLS AND FIXED-TIME ARTIFICIAL INSEMINATION (FTAI) IN DAIRY COWS

Suplicy, Miriane; Médica Veterinária Autonoma; Suplicy Filho, Haroldo; Médico Veterinário Autonomo; Kozicki, Luiz Ernandes; Pontificia Universidade Católica do Paraná; Breda, José Carlos dos Santos; Mestrado Ciencia Animal - PUCPR; Weiss, Romildo R
Fonte: UFPR Publicador: UFPR
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Artigo Avaliado pelos Pares Formato: application/pdf
Publicado em 09/08/2012 Português
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ABSTRACT: The aim of this study was to reduce the waiting voluntary period in dairy cows by administration of two different hormonal protocols for estrus synchronization and fixed-time artificial insemination (FTAI). One hundred twenty-eight Holstein dairy cows were studied. Three groups (G) were carried out: G1 (44 treated), G2 (34 treated) and G3 (50 as control). In the treated cows the following hormonal protocols was applied between 40 and 45 days post partum: G1- Day 0 (D0) - bovine intravaginal device containing progesterone (P4) plus 1.0 mg of estradiol benzoate (EB), D8 - removal of the P4, plus 0.5 mg of cloprostenol plus 400 IU of equine chorionic gonadotropin (eCG), D9 - 1.0 mg of EB and FTAI after 36 hours. G2 - D0 - P4 intravaginal plus 50 mcg of gonadotropin-releasing hormone (GnRH), D7 - removal of the P4 plus cloprostenol plus 400 IU eCG; D9 - 50 mcg of GnRH and FTAI 16 hours after. G3 – Control group inseminated 12 hours after the visual estrus. Results: G1 and G2 were inseminated (FTAI) respectively in the D55.5 and D56.8 after calving, while the G3 in the D84.5 (p<0.01).  The pregnancy rate in the G1, G2 and G3 was respectively 47.0, 46.1 and 35.8% (p>0.05).  In Conclusion: there was significant reduction in the voluntary waiting period in treated cows and higher pregnancy rate although not significant.; ABSTRACT: The aim of this study was to reduce the waiting voluntary period in dairy cows by administration of two different hormonal protocols for estrus synchronization and fixed-time artificial insemination (FTAI). One hundred twenty-eight Holstein dairy cows were studied. Three groups (G) were carried out: G1 (44 treated)...