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Caracterização e aplicação preliminares de um agente de contraste oral natural para imagens por ressonância magnética do trato gastrintestinal; Euterpe Olerácea (Açaí) as an Alternative Oral Contrast Agent in MR Imaging (MRI) of the Gastrointestinal (GI) System: Characterization and Clinical Preliminary Results

Sanchez, Tiago Arruda
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 08/04/2005 Português
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35.93%
O uso de agentes de contraste em técnicas de diagnóstico por imagem é uma prática médi-ca rotineira. Certos compostos, presentes em agentes de contraste, possuem propriedades paramagnéticas que podem afetar os sinais da tomografia por ressonância magnética, "Mag-netic Resonance Imaging"(MRI). Em estudos aplicados ao trato gastrintestinal (GI), os meios de contraste são amplamente utilizados por via endovenosa, mas também podem ser admi-nistrados oralmente. Porém, a adoção do uso oral é limitada, principalmente, porque os agentes convencionais são caros e causam, geralmente, efeitos colaterais. Desta forma, a-presentamos a caracterização e os resultados preliminares da implementação da polpa do fruto da Euterpe olerácea para um possível uso clínico como agente de contraste oral em MRI do trato GI. A polpa da Euterpe olerácea, conhecida como Açaí, de origem amazônica, apre-senta um aumento de sinal de MRI ponderado em T1 equivalente ao do Gd-DTPA e, tam-bém, um decréscimo de sinal em imagens ponderadas em T2. Investigamos propriedades intrínsecas que possam estar correlacionadas com o aumento de sinal em T1 e à opacidade em T2. O espectro de absorção atômica revelou a presença de íons Fe, Mn e Cu no Açaí...

Detecção da neoplasia do esôfago em pacientes com estenose por ingestão de agente corrosivo: estudo comparativo entre o emprego da técnica de cromoscopia óptica e o uso de cromoscopia de contraste; Detection of esophagus cancer in patients with caustic lesion/corrosive agent stenosis. A comparative study between narrow band imaging technique and chromoscopy with Lugol solution

Pennacchi, Caterina Maria Pia Simioni
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 09/10/2009 Português
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35.82%
Introdução: Narrow-Band Imaging (NBI) é uma das mais recentes técnicas de processamento de imagem, que consiste na utilização de filtros para dissociação das bandas do espectro da luz, resultando em aumento de contraste da superfície epitelial e da vascularização. Em combinação com a magnificação de imagem, pode-se diferenciar os tipos epiteliais e identificar áreas de padrão vascular decorrente de processos inflamatórios ou de neoplasias superficiais. A utilização da endoscopia associada à cromoscopia com solução de Lugol já é método consagrado na detecção de lesões superficiais em pacientes de alto risco, sendo largamente utilizado na prática diária. Objetivo: avaliar a aplicabilidade clínica da técnica de NBI na detecção e avaliação de neoplasias de esôfago em pacientes portadores de estenose do esôfago por agente corrosivo, comparando-a com a cromoscopia com solução de Lugol. Pacientes e Métodos: foram submetidos à avaliação com NBI 38 pacientes, sendo 22 e 16, com idade entre 28 84a (média M 56). O equipamento empregado é da linha de processadora Olympus, série EVIS II 180, Gastroscópio Olympus, série 180 GIF Type N180 slimsight ,com diâmetro 4.9mm, propiciando conforto ao paciente...

Acurácia diagnóstica da ecocardiografia sob estresse associada ao estudo da perfusão miocárdica com contraste na avaliação da isquemia miocárdica: estudo comparativo entre adenosina e dobutamina; Diagnostic accuracy of quantitative real time myocardial contrast echocardiography for the detection of myocardial ischemia. A comparative study between adenosine and dobutamine

Kowatsch, Ingrid
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 23/08/2005 Português
Relevância na Pesquisa
35.99%
A ecocardiografia com perfusão miocárdica em tempo real (EPMTR) permite a quantificação do fluxo sangüíneo miocárdico e, quando realizada durante o estresse, da reserva de fluxo miocárdico (reserva Axß). Essa técnica tem potencial para ser uma importante ferramenta para o diagnóstico não-invasivo da doença arterial coronariana (DAC). Apesar do conhecimento atual das alterações fisiológicas que ocorrem com o uso de agentes vasodilatadores ou catecolaminas na circulação coronariana, não há dados na literatura comparando diretamente o valor da EPMTR, sob estresse pela dobutamina e pela adenosina, para a detecção de DAC em humanos. Os objetivos deste estudo foram: avaliar, em um mesmo grupo de pacientes, a exeqüibilidade e a acurácia da EPMTR, sob estresse pela dobutamina e pela adenosina, para a detecção de estenose arterial coronariana angiograficamente significativa e determinar o valor adicional da análise quantitativa da perfusão miocárdica sobre o eletrocardiograma de 12 derivações, da motilidade segmentar e da análise qualitativa da perfusão miocárdica obtidas durante o estresse pela dobutamina e pela adenosina. Estudamos 54 pacientes (média etária de 60±9 anos, 33 homens) com suspeita clínica de DAC e indicação de angiografia coronariana. Todos os pacientes foram submetidos à EPMTR sob estresse pela adenosina na dose de 140 g/kg/min por seis minutos e...

Atributos diagnósticos da reação em cadeia pela polimerase com oligonucleotídeos iniciadores direcionados a genoma de cinetoplasto e nuclear de Leishmania spp; Diagnostic attributes of the polymerase chain reaction with primers targeted to the kinetoplast and nuclear genome of Leishmania spp.

Lopes, Estela Gallucci
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 08/10/2010 Português
Relevância na Pesquisa
36.12%
A técnica de PCR pode ser empregada para a detecção e identificação de agentes patogênicos e, tem por isso grande aplicabilidade em estudos de epidemiologia molecular. Particularmente no que se refere às infecções por Leishmania spp., a detecção do seu agente é de suma importância em animais sorologicamente positivos, no caso de inquéritos para a detecção direta em vetores e em animais de vida livre, cujo anti-soro para provas sorodiagnósticas, não são disponíveis. A PCR ainda pode oferecer o recurso de identificação molecular do agente em pesquisa, quando marcadores filogeneticamente informativos são amplificados e seqüenciados. Neste sentido, foi realizado o presente trabalho, cujo objetivo foi avaliar o desempenho analítico e diagnóstico de PCRs baseadas em primers direcionados a marcadores universais para diagnosticar Leishmania spp. localizados em dois genomas da célula parasitária, a saber, kDNA (maxicírculo e minicírculo) e DNA nuclear. Foram avaliadas PCRs baseadas em primers direcionados ao (i) DNA de kinetoplasto (kDNA) minicirculo, ao gene codificador de Citocromo B e Citocromo C Oxidase subunidade II presentes no (ii) kDNA maxicírculo, e ao gene codificador Citicromo C, presente no (iii) DNA nuclear. Pelos resultados infere-se que a PCR direcionada ao kDNA de minicírculo apresentou uma sensibilidade analítica muito superior às PCRs direcionadas ao DNA maxicirculo e nuclear. Pelo menos uma combinação dos primers de cada marcador foi capaz de detectar DNA de todas as espécies da Coleção de Leishmania do Instituto Oswaldo Cruz (CLIOC)...

The diagnostic value of Gram stain for initial identification of the etiologic agent of peritonitis in CAPD patients

Bezerra, Dinoráh A.; Silva, Mario B.; Caramori, Jaqueline S.T.; Sugizaki, Maria F.; Sadatsune, Teruê; Montelli, Augusto C.; Barretti, Pasqual
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 269-272
Português
Relevância na Pesquisa
36.12%
Objective: To evaluate the effectiveness of the Gram stain in the initial diagnosis of the etiologic agent of peritonitis in continuous ambulatory peritoneal dialysis (CAPD). Design: Retrospective study analyzing the sensitivity (S), specificity (SS), positive predictive value (+PV), and negative predictive value (-PV) of the Gram stain relating to the results of cultures in 149 episodes of peritonitis in CAPD. The data were analyzed in two studies. In the first, only the cases with detection of a single agent by Gram stain were taken (Study 1). In the second, only the cases with two agents in Gram stain were evaluated (Study 2). Setting: Dialysis Unit and Laboratory of Microbiology of a tertiary medical center. Patients: Sixty-three patients on regular CAPD who presented one or more episodes of peritonitis from May 1992 to May 1995. Results: The positivity of Gram stain was 93.2% and the sensitivity was 95.7%. The values of S, SS, +PV, and -PV were respectively: 94.9%, 53.5%, 68.3%, and 90.9% for gram-positive cocci and 83.3%, 98.8%, 95.2%, and 95.6% for gram-negative bacilli. The association of gram-positive cocci plus gram-negative bacilli were predictive of growth of both in 6.8%, growth of gram-positive cocci in 13.7%, and growth of gram-negative bacilli in 72.5%. Conclusions: The Gram stain is a method of great value in the initial diagnosis of the etiologic agent of peritonitis in CAPD...

Marginal fit of nickel-chromium copings before and after internal adjustments with duplicated stone dies and disclosing agent.

Ushiwata, O.; de Moraes, J. V.; Bottino, M. A.; da Silva, E. G.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 634-643
Português
Relevância na Pesquisa
45.95%
STATEMENT OF PROBLEM: Despite careful procedures, master stone dies may be damaged during laboratory procedures. The dentist routinely adjusts castings because the marginal fit of casting is not as accurate as on the dies. PURPOSE: This study evaluated the technique of internal adjustment of castings with use of duplicated stone dies and a disclosing agent to improve marginal fit discrepancy. MATERIAL AND METHODS: Thirty-two nickel-chromium copings were fabricated and simulated standard clinical and laboratory procedures with 2 variables: tooth preparation convergence angles of 6 and 18 degrees, with or without internal relief. Master stone dies and their duplicates were selected for coping construction and internal adjustment, respectively. A specimen positioning device was coupled with a Toolmakers microscope to allow reproducibility of measurements. Each coping was evaluated at 8 locations of its marginal perimeter, before and after internal adjustment. RESULTS: Marginal fit discrepancy of copings were significantly reduced with an internal adjustment technique (mean > 52%) for all experimental groups. Tooth preparations with greater convergence and internally relieved castings recorded a better marginal fit. CONCLUSION: The casting internal adjustment technique with use of duplicated stone dies and a disclosing agent substantially reduced marginal fit discrepancy.

Relationship between gingival clinical parameters and the reactivity of the BANA test in subgingival samples from children.

Zuza, Elizangela Partata; de Salis, Ana Maria Vilela; de Toledo, Benedicto Egbert Corrêa; Mendes, Ary José Dias
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 78-82
Português
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35.81%
Gingivitis is the first manifestation of periodontal disease, and is characterized by painless and slow evolution. Early diagnosis and intervention must be done to avoid the possibility of precocious periodontitis during the childhood or teenage years. The enzymatic BANA test (N-benzoyl-DL-arginine-naphthylamide) was used to evaluate subgingival samples from 54 children between 6 and 9 years of age. Plaque index (PI) and gingival index (GI) were assessed according to the criteria recommended by Löe (1967). Subgingival plaque was collected from the region that featured the greatest periodontal alteration, represented by a higher gingival index. Resulting data were grouped individually according to visible and non-visible plaque and bleeding and non-bleeding gingiva. Results showed that there was no statistically significant correlation between the presence of visible plaque and the positivity of the BANA test, nor was there a statistically significant correlation between the presence of bleeding and the positivity of the BANA test in subgingival samples obtained from children. This study concluded that the BANA test is not an ideal diagnostic test to be applied to children.

A multi-agent based medical image multi-display visualization system

Alves, Victor; Marreiros, Filipe; Nelas, Luís
Fonte: IPP, ISEP Publicador: IPP, ISEP
Tipo: Artigo de Revista Científica
Publicado em //2006 Português
Relevância na Pesquisa
35.93%
The evolution of equipments used in the medical imaging practice, from 3-tesla Magnetic Resonance (MR) units and 64-slice Computer Tomography (CT) systems to the latest generation of hybrid Positron Emission Tomography (PET)/CT technologies is fast producing a volume of images that threatens to overload the capacity of the interpreting radiologists. On the other hand multi-agents systems are being used in a wide variety of research and application fields. Our work concerns the development of a multi-agent system that enables a multi-display medical image diagnostic system. The multi-agent system architecture permits the system to grow (scalable) i.e., the number of displays according to the user’s available resources. There are two immediate benefits of this scalable feature: the possibility to use inexpensive hardware to build a cluster system and the real benefit for physicians is that the visualization area increases allowing for easier and faster navigation. In this way an increase in the display area can help a physician analyse and interpret more information in less time

Comparison of three diagnostic techniques for the detection of leptospires in the kidneys of wild house mice (Mus musculus)

Rossetti,Carlos A.; Vanasco,Bibiana N.; Pini,Noemí; Carfagnini,Julio C.
Fonte: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Publicador: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2004 Português
Relevância na Pesquisa
35.81%
Forty-one wild house mice (Mus musculus) were trapped in an urban area, near railways, in Santa Fe city, Argentina. Both kidneys from each mouse were removed for bacteriological and histological examination. One kidney was inoculated into Fletcher semi-solid medium and isolates were serologically typed. The other kidney was microscopically examined after hematoxylin-eosin, silver impregnation and immunohistochemical stains. Leptospires, all of them belonging to the Ballum serogroup, were isolated from 16 (39%) out of 41 samples. The presence of the agent was recorded in 18 (44%) and in 19 (46%) out of 41 silver impregnated and immunohistochemically stained samples respectively. Additionally, leptospires were detected in high number on the apical surface of epithelial cells and in the lumen of medullary tubules and they were less frequently seen on the apical surface of epithelial cells or in the lumen of the cortical tubules, which represents an unusual finding in carrier animals. Microscopic lesions consisting of focal mononuclear interstitial nephritis, glomerular shrinkage and desquamation of tubular epithelial cells were observed in 13 of 19 infected and in 10 of 22 non-infected mice; differences in presence of lesions between infected and non-infected animals were not statistically significant (P=0...

Design and synthesis of a siderophore conjugate as a potent PSMA inhibitor and potential diagnostic agent for prostate cancer

Ding, Pingyu; Helquist, Paul; Miller, Marvin J.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
45.95%
A Siderophore conjugate was designed as a potential PSMA inhibitor and diagnostic agent for prostate cancer. A semi-rigid spacer was incorporated to avoid competitive participation of iron binding by the enzyme inhibitor relative to the siderophore component. Biological test results showed that, even with the extended scaffold, this compound is a potent PSMA inhibitor with an IC50 of 4 nM. This siderophore conjugate may be useful for detection of prostate-derived cancer cells by magnetic resonance imaging (MRI).

ATPace™: injectable adenosine 5′-triphosphate: Diagnostic and therapeutic indications

Pelleg, Amir; Kutalek, Steven P.; Flammang, Daniel; Benditt, David
Fonte: Springer Netherlands Publicador: Springer Netherlands
Tipo: Artigo de Revista Científica
Português
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36.05%
ATPace™, a novel injectable formulation of adenosine 5′-triphosphate (ATP), is developed by Cordex Pharma, Inc. (Cordex) as a diagnostic and therapeutic drug for the management of cardiac bradyarrhythmias. Extracellular ATP exerts multiple effects in various cell types by activating cell-surface receptors known as P2 receptors. In the heart, ATP suppresses the automaticity of cardiac pacemakers and atrioventricular (AV) nodal conduction via adenosine, the product of its degradation by ecto-enzymes, as well as by triggering a cardio-cardiac vagal reflex. ATP, given as a rapid intravenous bolus injection, has been used since the late 1940s as a highly effective and safe therapeutic agent for the acute termination of reentrant paroxysmal supraventricular tachycardia (PSVT) involving the AV node. In addition, preliminary studies have shown that ATP can also be used as a diagnostic agent for the identification of several cardiac disorders including sinus node dysfunction (sick sinus syndrome), dual AV nodal pathways, long QT syndrome, and bradycardic syncope. The US Food and Drug Administration has approved Cordex formulation for ATP as an Investigational New Drug and two pathways for its marketing approval; one therapeutic, i.e., acute termination of paroxysmal PSVT...

Use of a Single Hybrid Imaging Agent for Integration of Target Validation with In Vivo and Ex Vivo Imaging of Mouse Tumor Lesions Resembling Human DCIS

Buckle, Tessa; Kuil, Joeri; van den Berg, Nynke S.; Bunschoten, Anton; Lamb, Hildo J.; Yuan, Hushan; Josephson, Lee; Jonkers, Jos; Borowsky, Alexander D.; van Leeuwen, Fijs W. B.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.06%
Screening of biomarker expression levels in tumor biopsy samples not only provides an assessment of prognostic and predictive factors, but may also be used for selection of biomarker-specific imaging strategies. To assess the feasibility of using a biopsy specimen for a personalized selection of an imaging agent, the chemokine receptor 4 (CXCR4) was used as a reference biomarker. Methods: A hybrid CXCR4 targeting peptide (MSAP-Ac-TZ14011) containing a fluorescent dye and a chelate for radioactive labeling was used to directly compare initial flow cytometry–based target validation in fresh tumor tissue to (in) (vivo) single photon emission computed tomography (SPECT) imaging and (in) (vivo) and (ex) (vivo) fluorescence imaging. Results: Flow cytometric analysis of mouse tumor derived cell suspensions enabled discrimination between 4T1 control tumor lesions (with low levels of CXCR4 expression) and CXCR4 positive early, intermediate and late stage MIN-O lesions based on their CXCR4 expression levels; CXCR4(^{basal}), CXCR4(^+) and CXCR4(^{++}) cell populations could be accurately discriminated. Mean fluorescent intensity ratios between expression in MIN-O and 4T1 tissue found with flow cytometry were comparable to ratios obtained with in vivo SPECT/CT and fluorescence imaging...

Pricing Diagnostic Information

Arora, Ashish; Fosfuri, Andrea
Fonte: INFORMS (Institute for Operations Research and Management Sciences) Publicador: INFORMS (Institute for Operations Research and Management Sciences)
Tipo: Artigo de Revista Científica Formato: application/pdf
Publicado em /07/2005 Português
Relevância na Pesquisa
35.97%
Diagnostic information allows an agent to predict the state of nature about the success of an investment project better than the prior. We analyze the optimal pricing scheme for selling diagnostic information to buyers with different, privately known, ex ante success probability. Investment costs and returns of successful projects are assumed to be the same for all buyers. The value of diagnostic information is the difference in expected payoffs with and without it, and we show that the willingness to pay for diagnostic information is nonmonotonic in the ex ante success probability. When the information seller can offer only one quality level, and negative payments are not allowed, we find that the optimal menu of (linear) contracts is remarkably simple. A pure royalty is offered to buyers with low ex ante success probability, and a pure fixed fee is offered to buyers with high ex ante success probability.

Pricing Diagnostic Information

Arora, Ashish; Fosfuri, Andrea
Fonte: Universidade Carlos III de Madrid Publicador: Universidade Carlos III de Madrid
Tipo: Trabalho em Andamento Formato: application/pdf
Publicado em 18/01/2005 Português
Relevância na Pesquisa
35.97%
Diagnostic information allows an agent to predict the state of nature about the success of an investment project better than the prior. We analyze the optimal pricing scheme for selling diagnostic information to buyers with different, privately known, ex ante success probability. Investment costs and returns of successful projects are assumed to be the same for all buyers. The value of diagnostic information is the difference in expected payoffs with and without it, and we show that the willingness to pay for diagnostic information is non monotonic in the ex ante success probability. When the information seller can offer only one quality level, and negative payments are not allowed, we find that the optimal menu of (linear) contracts is remarkably simple. A pure royalty is offered to buyers with low ex ante success probability and a pure fixed fee is offered to buyers with high ex ante success probability.

Facteurs de risque d´introduction et diagnostic de Mycobacterium avium ssp. paratuberculosis

Rangel Valderrama, Saray Julieth
Fonte: Université de Montréal Publicador: Université de Montréal
Tipo: Thèse ou Mémoire numérique / Electronic Thesis or Dissertation
Português
Relevância na Pesquisa
35.97%
Mycobacterium avium ssp. paratuberculosis (MAP) est l'agent causal de la paratuberculose, maladie entérique, chronique et incurable des ruminants, avec un impact économique important. Une meilleure compréhension des facteurs de risque associés à l'introduction de la maladie dans un troupeau est essentielle pour sa prévention. L’amélioration des tests diagnostiques est aussi importante pour son contrôle. L’introduction des nouveaux animaux dans le troupeau et la présence et contact des différentes espèces sauvages et domestiques avec les vaches, semblent être des facteurs de risques d’introduction de la maladie. Nous avons réalisé une revue systématique dont l`objective était de recueillir l’information pertinente pour répondre à la question sur l’importance de ces facteurs et leur impact sur l’introduction de la maladie dans un troupeau. D`un autre côté, la détection de MAP dans les fèces par culture bactérienne demeure la méthode diagnostique de choix malgré les facteurs qui l`affectent. Une série de 3 étapes est requise afin de confirmer la présence du MAP : (1) culture (2) coloration, et (3) confirmation du MAP par PCR (si détecté à l´étape 2). Certains échantillons fécaux présentent une particularité en raison de leur forte charge de micro-organismes. Ces contaminants peuvent interférer avec la croissance et la détection de MAP. Une étude visant à : a) estimer l'impact des certain covariables sur les résultats de la culture de MAP parmi l`analyse rétrospective d`un banque des données et b) évaluer la possibilité d'optimiser le processus de diagnostic du MAP en effectuant l'analyse PCR sur les cultures déclarées comme contaminées a été réalisée.; Mycobacterium avium ssp. paratuberculosis (MAP) is the causative agent of paratuberculosis an enteric...

Apport de l’expertise d’un hygiéniste au diagnostic de l’asthme professionnel

de Olim Rugginenti, Carlo
Fonte: Université de Montréal Publicador: Université de Montréal
Tipo: Thèse ou Mémoire numérique / Electronic Thesis or Dissertation
Português
Relevância na Pesquisa
36.24%
Introduction : L’asthme professionnel (AP) est diagnostiqué au Québec avec le test de provocation bronchique spécifique (TPS). Le TPS consiste à exposer le patient à un agent causal suspecté en vue de provoquer une réaction asthmatique. Un TPS négatif est possible quand un agent causal a été omis de l’histoire professionnelle du patient. L’évaluation des expositions professionnelles par une expertise en hygiène en santé du travail est considérée comme une méthode précise, lorsque des données de mesure ne sont pas disponibles. Cependant, l'apport de cette méthode dans le diagnostic de l’AP n'a jamais été examiné dans un contexte clinique. Objectifs : Déterminer l'apport de l'évaluation des expositions professionnelles par une expertise en hygiène du travail dans l'investigation de l'AP. Comparer les expositions professionnelles détectées par un clinicien et par un hygiéniste chez 1) des sujets avec de l’AP prouvé par des TPS positifs, 2) chez des sujets avec des TPS négatifs. Méthodes : Une analyse des expositions potentielles par le clinicien a précédé la réalisation du TPS. Une évaluation des expositions professionnelles a été réalisée par un hygiéniste. L’hygiéniste n’avait pas connaissance du diagnostic du patient. Résultats : 120 sujets (TPS positifs : 67 négatifs :53) ont été enrôlés dans l’étude. L’hygiéniste a identifié l’agent causal dans la très grande majorité des TPS positifs. Dans 33 TPS négatifs...

Prevalência de parasitos intestinais em gatos errantes em Goiânia – Goiás: ênfase no diagnóstico de Toxoplasma gondii e avaliação da acurácia de técnicas parasitológicas; Prevalence of intestinal parasites in stray cats in Goiânia, Goiás: emphasis of diagnostic Toxoplasma gondii and evaluotion accuracy parasitology techniques

Rezende, Hanstter Hallison Alves
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP); Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP); Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Tipo: Dissertação Formato: application/pdf
Português
Relevância na Pesquisa
35.88%
The domestic cat is an important zoonotic agent for humans, especially as a transmissor of Toxoplasma gondii. The cat is capable of the elimination of millions of oocysts in the environment leading to a massive contamination of these places and leading to a public health issue. Toxoplamosis is a disease with high prevalence throughout the world and the cat is responsible for the parasite’s perpetuation. The evaluation of the accuracy of parasitological diagnostic methods is of vital importance to improve the veterinarian laboratory diagnosis through the use of high sensitivity, specificity and reproducible tests. The aim of this study was to verify the prevalence of intestinal parasites from stray cats in Goiania-GO, to verify the seroprevalence of anti-T. gondii antibodies through the modified aglutination test (MAT) and to perform the evaluation of the accuracy of the parasitological tests applied. Therefore 155 samples of feces and 50 blood samples from stray cats from the Zoonosis Center of Goiania were collected during the year of 2012. The feces samples were processed by the Willis (gold standard), Sheather, Faust and Hoffman-Janer-Pons-Lutz (HJPL) techniques. The MAT was performed in the blood samples. The accuracy was performed through the determination of sensitivity...

Colaboração em ambientes inteligentes de aprendizagem mediada por um agente social probabilístico; Collaboration in intelligent learning environments supported by a probabilistic social agent

Boff, Elisa
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Tese de Doutorado Formato: application/pdf
Português
Relevância na Pesquisa
36.16%
Este trabalho propõe um modelo probabilístico de conhecimento e raciocínio para um agente, denominado Agente Social, cujo principal objetivo é analisar o perfil dos alunos, usuários de um Sistema Tutor Inteligente chamado AMPLIA, e compor grupos de trabalho. Para formar estes grupos, o Agente Social considera aspectos individuais do aluno e estratégias de formação de grupos. A aprendizagem colaborativa envolve relações sociais cujos processos são complexos e apresentam dificuldade para sua modelagem computacional. A fim de representar alguns elementos deste processo e de seus participantes, devem ser considerados aspectos individuais, tais como estado afetivo, questões psicológicas e cognição. Também devem ser considerados aspectos sociais, tais como a habilidade social, a aceitação e a forma em que as pessoas se relacionam e compõem seus grupos de trabalho ou estudo. Sistemas Tutores Inteligentes, Sistemas Multiagente e Computação Afetiva são áreas de pesquisa que vem sendo investigadas de forma a oferecer alternativas para representar e tratar computacionalmente alguns destes aspectos multidisciplinares que acompanham a aprendizagem individual e colaborativa. O Agente Social está inserido na sociedade de agentes do portal PortEdu que...

Rational design of a peptide capture agent for CXCL8 based on a model of the CXCL8:CXCR1 complex

Helme, Dorothea; Rink, Ina; Dalton, James A.R.; Brahm, Kevin; Marina Jöst; Nargang, Tobias M.; Blum, Witali; Wadhwani, Parvesh; Brenner-Weiss, Gerald; Rapp, Bastian E.; Giraldo, Jesús; Schmitz, Katja
Fonte: Universidade Autônoma de Barcelona Publicador: Universidade Autônoma de Barcelona
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/acceptedVersion
Publicado em //2015 Português
Relevância na Pesquisa
35.93%
Protein-capture agents are widely used for the detection, immobilization and isolation of proteins and are the foundation for the development of in vitro diagnostic chips. The chemokine CXCL8 is an interesting protein target due to its involvement in the human inflammatory response. We constructed a novel structural model of CXCL8 interaction with its G-protein coupled receptor CXCR1, taking into account previously reported experimental data. From this CXCL8:CXCR1 model complex, the interaction of CXCL8 with residues near the extracellular domains 3 and 4 of CXCR1 were used as a scaffold for the rational design of a peptide capture agent called ‘IL8RPLoops’. A molecular dynamics simulation of IL8RPLoops indicates a stable helical conformation consistent with the CXCR1 structure from which it was derived. CXCL8 capture in fluorescence-based assays on beads and on glass demonstrates that IL8RPLoops is an effective capture agent for CXCL8. Additionally, we found IL8RPLoops to be a potent inhibitor of CXCL8-induced neutrophil migration and CXCL8:CXCR1 association. A theoretical binding model for IL8RPLoops:CXCL8 is proposed, which shows the peptide predominantly interacting with CXCL8 via electrostatic contacts with the ELR motif at the CXCL8 N-terminus.

The Potential for Ultrasonic Image-Guided Therapy Using a Diagnostic System

Bing, Kristin Frinkley
Fonte: Universidade Duke Publicador: Universidade Duke
Tipo: Dissertação Formato: 5492058 bytes; application/pdf
Publicado em 13/11/2008 Português
Relevância na Pesquisa
36.14%

Ultrasound can be used for a variety of therapeutic purposes. High-intensity focused ultrasound (HIFU) has progressed over the past decade to become a viable therapeutic method and is valuable as a non-invasive alternative to many surgical procedures. Ultrasonic thermal therapies can also be used to release thermally sensitive liposomes encapsulating chemotherapeutic drugs. In the brain, the permeability of the blood-brain barrier to drugs, antibodies, and gene transfer can be increased with a mechanical mechanism using ultrasound and contrast agent.

The work presented in this dissertation tests the hypothesis that a diagnostic system can be used for combined imaging and therapeutic applications. In order to evaluate the effectiveness of a diagnostic system for use in therapeutic applications, a set of non-destructive tests is developed that can predict the potential for high acoustic output. A rigorous, nondestructive testing regimen for standard, diagnostic transducers to evaluate their potential for therapeutic use is formulated. Based on this work, transducer heating is identified as the largest challenge. The design and evaluation of several custom diagnostic transducers with various modifications to reduce internal heating are described. These transducers are compared with diagnostic controls using image contrast...