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Detection of salmonella sp. from porcine origin: a comparision between a PCR method and standard microbiological techniques.; Detecção de Salmonella sp. em amostras de origem suína: comparação entre a técnica da Reação em Cadeia da Polimerase e o isolamento bacteriano convencional

Castagna, Sandra Maria Ferraz; Müller, Monika; Macagnan, Marisa; Rodenbusch, Carla Rosane; Canal, Cláudio Wageck; Cardoso, Marisa Ribeiro de Itapema
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
Relevância na Pesquisa
46.06%
O objetivo desse estudo foi comparar um método de Reação em Cadeia da Polimerase (PCR) combinado com enriquecimento seletivo em caldo Rappaport-Vassiliadis (PCR-RVB) com as técnicas de isolamento bacteriano convencional (SMT) para a detecção do gênero Salmonella em amostras de origem suína. Duzentas e sessenta e oito amostras de campo, compostas por: 42 “pools” de linfonodos mandibulares e tonsilas, 44 amostras de conteúdo intestinal, 38 amostras de massa de embutidos e 144 amostras de ração coletadas em granjas foram submetidas ao protocolo de PCR-RVB e SMT. Salmonella foi detectada em 54 amostras usando o PCR-RVB e em 42 amostras pelo SMT; três amostras positivas no SMT (isolados de, respectivamente, S. Derby, S. Panama e S. Typhimurium) foram negativas no PCRRVB. Quinze amostras positivas no PCR-RVB foram negativas no SMT, uma diferença considerada significativa de acordo com o teste de Mac Nemar (p=0,0153). A tipificação antigênica dos isolados do SMT revelou a presença dos seguintes sorovares de Salmonella, sendo demonstrado entre parênteses o número de isolados: Typhimurium (12); Bredeney (10); Panama (5); Saint-paul (5); Minnesota (3); Mbandaka (2); Derby (1); Enteritidis (1); Orion (1) e Salmonella sp. (2). Concluiu-se que...

Avaliação da reação em cadeia da polimerase e do isolamento bacteriológico convencional na detecção de Salmonella Dublin em amostras de fezes de bezerros infectados experimentalmente

Silva, D. G.; Silva, D. R.; Silva, P. R. L.; Cícero, E. A. S.; Ferraz, A.L.J.; Lemos, M. V. F.; Fagliari, J. J.
Fonte: Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária Publicador: Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária
Tipo: Artigo de Revista Científica Formato: 752-756
Português
Relevância na Pesquisa
26.27%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); The efficiency of the Polymerase Chain Reaction (PCR) combined with selective enrichment broth was compared with the standard microbiological techniques for detection of Salmonella Dublin in fecal samples of 10 to 15-days-old Holstein calves, experimentally infected with 10(8) CFU of Salmonella Dublin. Seventy-six fecal samples were analyzed using PCR associated with selenite cystine (SC) and Muller-Kauffmann tetrathionate (TMK) broths and standard microbiological techniques. Regardless of the selective enrichment broth used, the standard microbiological techniques were significantly better than PCR in detection of positive samples of Salmonella Dublin. However, the simultaneous use of both techniques provided detection of a larger number of positive samples. The SC broth was the best option as selective enrichment in both techniques.

Collection of tracheal aspirate: safety and microbiological concordance between two techniques

Frota,Oleci Pereira; Ferreira,Adriano Menis; Barcelos,Larissa da Silva; Watanabe,Evandro; Carvalho,Nádia Cristina Pereira; Rigotti,Marcelo Alessandro
Fonte: Universidade de São Paulo, Escola de Enfermagem Publicador: Universidade de São Paulo, Escola de Enfermagem
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2014 Português
Relevância na Pesquisa
36.2%
Objective: To evaluate the safety of the performance of the traditional and protected collection techniques of tracheal aspirate and to identify qualitative and quantitative agreement of the results of microbiological cultures between the techniques. Method: Clinical, prospective, comparative, single-blind research. The sample was composed of 54 patients of >18 years of age, undergoing invasive mechanical ventilation for a period of ≥48 hours and with suspected Ventilator Associated Pneumonia. The two techniques were implemented in the same patient, one immediately after the other, with an order of random execution, according to randomization by specialized software. Results: No significant events occurred oxygen desaturation, hemodynamic instability or tracheobronchial hemorrhage (p<0.05) and, although there were differences in some strains, there was qualitative and quantitative agreement between the techniques (p<0.001). Conclusion: Utilization of the protected technique provided no advantage over the traditional and execution of both techniques was safe for the patient.


Determination of moisture content and water activity in algae and fish by thermoanalytical techniques

Silva,Vilma Mota da; Silva,Luciana Almeida; Andrade,Jailson B. de; Veloso,Márcia C. da Cunha; Santos,Gislaine Vieira
Fonte: Sociedade Brasileira de Química Publicador: Sociedade Brasileira de Química
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2008 Português
Relevância na Pesquisa
35.93%
The water content in seafoods is very important since it affects their sensorial quality, microbiological stability, physical characteristics and shelf life. In this study, thermoanalytical techniques were employed to develop a simple and accurate method to determine water content (moisture) by thermogravimetry (TG) and water activity from moisture content values and freezing point depression using differential scanning calorimetry (DSC). The precision of the results suggests that TG is a suitable technique to determine moisture content in biological samples. The average water content values for fish samples of Lutjanus synagris and Ocyurus chrysurus species were 76.4 ± 5.7% and 63.3 ± 3.9%, respectively, while that of Ulva lactuca marine algae species was 76.0 ± 4.4%. The method presented here was also successfully applied to determine water activity in two species of fish and six species of marine algae collected in the Atlantic coastal waters of Bahia, in Brazil. Water activity determined in fish samples ranged from 0.946 - 0.960 and was consistent with values reported in the literature, i.e., 0.9 - 1.0. The water activity values determined in marine algae samples lay within the interval of 0.974 - 0.979.

Training program on microbiological test collection material methods at a teaching hospital: investment and result assessment

Jericó,Marli de Carvalho; Castilho,Valéria; Perroca,Márcia Galan
Fonte: Escola de Enfermagem de Ribeirão Preto / Universidade de São Paulo Publicador: Escola de Enfermagem de Ribeirão Preto / Universidade de São Paulo
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2006 Português
Relevância na Pesquisa
36.05%
This study aimed at evaluating the results, direct costs and investment of a training program on microbiological test material collection at a teaching hospital. Test collections that did not follow the established criteria (failure) were considered as the result measure. Variable and absorption costing were used to calculate direct costs and investments, respectively. Of the 11,893 collected materials, failures were evidenced in 59 (0.5%). Direct cost corresponded to R$ 154.10 and R$ 2,431.29 was invested in training. These findings revealed that the evidenced number of anomalies (failures) represented a low percentage in relation to the total collected material for microbiological exams. Therefore, this should not be considered a critical point that justifies the continuity of the training and, consequently, the investment.

Electron Microscopy of the Cell Envelope of Ferrobacillus ferrooxidans Prepared by Freeze-Etching and Chemical Fixation Techniques

Remsen, Charles; Lundgren, D. G.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /12/1966 Português
Relevância na Pesquisa
35.93%
Remsen, C. C. (Swiss Federation Institute of Technology, Zurich, Switzerland), and D. G. Lundgren. Electron microscopy of the cell envelope of Ferrobacillus ferrooxidans prepared by freeze-etching and chemical fixation techniques. J. Bacteriol. 92:1765–1771. 1966.—A comparison was made of the fine structure of the cell envelope of the gram-negative bacterium Ferrobacillus ferrooxidans when cells were prepared for microscopy by freeze-etching and chemical fixation techniques. Cell envelopes of chemically fixed cells appeared as five separate layers distinguishable by their location and electron density. Frozen-etched cells showed a three-layered complex with each layer measuring approximately 100 A in thickness. The latter technique is considered to be “artifact-free” and, as a technique, yields purely morphological information on the natural state. The three layers revealed by freeze-etching are: the outer layer, a lipoprotein-lipopolysaccharide layer; the middle layer, a layer composed of globular protein attached to fibrillar mucopeptide; and the innermost layer, the cytoplasmic membrane. The latter was covered with 100 to 120 A particles. The relationship of the aforementioned layers to those seen in chemically fixed cells is discussed.

The Compu-pet 100: a versatile dispenser-diluter for the mechanization of microbiological techniques.

Cremer, A W; Mellars, B; Stokes, E J
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /01/1975 Português
Relevância na Pesquisa
36.06%
The Compu-pet 100 diluter/dispenser is a versatile and accurate instrument which can be employed for a wide variety of microbiological tests and techniques. Time in performing tests is often more than halved when the diluter is used. Large numbers of tests can be performed with minimal fatigue.

Improved impression cytology techniques for the immunopathological diagnosis of superficial viral infections

Thiel, M; Bossart, W; Bernauer, W
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1997 Português
Relevância na Pesquisa
35.97%
BACKGROUND—For epidemiological and therapeutic reasons early diagnosis of superficial viral infections is crucial. Conventional microbiological techniques are expensive, time consuming, and not sufficiently sensitive. In this study impression cytology techniques were evaluated to analyse their diagnostic potential in viral infections of the ocular surface.
METHOD—A Biopore membrane device instead of the original impression cytology technique was used to allow better quality and handling of the specimens. The impressions were processed, using monoclonal antibodies and immunoperoxidase or immunofluorescence techniques to assess the presence of herpes simplex virus, varicella zoster virus, or adenovirus antigens. Ocular surface specimens from healthy individuals (n=10) and from patients with suspected viral surface disease (n=19) were studied. Infected and non-infected cell cultures served as controls.
RESULTS—This modified technique of impression cytology allowed the collection of large conjunctival and corneal epithelial cell layers with excellent morphology. Immunocytological staining of these samples provided diagnostic results for all three viruses in patients with viral surface disease.
CONCLUSIONS—The use of Biopore membrane devices for the collection of ocular surface epithelia offers new diagnostic possibilities for external eye diseases. Immunopathological methods that are applied directly on these membrane devices can provide virological results within 1-4 hours. This contributes considerably to the clinical management of patients with infectious diseases of the ocular surface.



Clinico microbiological evaluation of surface and core microflora in chronic tonsillitis

Mallya, P. Shrikara; Abraham, Bindu
Fonte: Springer India Publicador: Springer India
Tipo: Artigo de Revista Científica
Publicado em /07/1998 Português
Relevância na Pesquisa
35.96%
A total of 100 patients with chronic tonsillitis were selected for this study. Specimens collected immediately before surgery and the operated specimen of tonsils were subjected to appropriate microbiological techniques. The commonest isolate on the surface was Streptococcus pneumoniae and in the core group a streptococcus. The largest group was the one in which surface and core pathogens were the same. At the same time it is interesting to note that surface culture was misleading as to the presence or absence of core pathogens in 32% of the study group. The core only was pathogenic in 12% and the group which isolated a different pathogen on the surface and core was 20%. It is evident that in a significant group of patients surface cultures were not useful in predicting core pathogens.

Clinical and microbiological diagnosis of oral candidiasis

Coronado-Castellote, Laura; Jiménez-Soriano, Yolanda
Fonte: Medicina Oral S.L. Publicador: Medicina Oral S.L.
Tipo: Artigo de Revista Científica
Publicado em 01/12/2013 Português
Relevância na Pesquisa
36.25%
Introduction: Candidiasis or oral candidiasis is the most frequent mucocutaneous mycosis of the oral cavity. It is produced by the genus Candida, which is found in the oral cavity of 53% of the general population as a common commensal organism. One hundred and fifty species have been isolated in the oral cavity, and 80% of the isolates correspond to Candida albicans, which can colonize the oral cavity alone or in combination with other species. Transformation from commensal organism to pathogen depends on the intervention of different predisposing factors that modify the microenvironment of the oral cavity and favor the appearance of opportunistic infection. The present study offers a literature review on the diagnosis of oral candidiasis, with the purpose of establishing when complementary microbiological techniques for the diagnosis of oral candidiasis should be used, and which techniques are most commonly employed in routine clinical practice in order to establish a definitive diagnosis. Materials and methods: A Medline-PubMed, Scopus and Cochrane search was made covering the last 10 years. Results: The diagnosis of oral candidiasis is fundamentally clinical. Microbiological techniques are used when the clinical diagnosis needs to be confirmed...

Microbiological evaluation of endodontic files after cleaning and steam sterilization procedures

VanEldik, D.; Zilm, P.; Rogers, A.; Marin, P.
Fonte: Australian Dental Assn Inc Publicador: Australian Dental Assn Inc
Tipo: Artigo de Revista Científica Formato: 72806 bytes; application/pdf
Publicado em //2004 Português
Relevância na Pesquisa
35.96%
Background: Infection control procedures are essential for modern dental practice and they are continually evolving to meet the dental profession’s high standards. The present study evaluated the efficacy of two cleaning procedures to reduce bacterial numbers on endodontic files, and evaluated the effect of biological debris on the subsequent sterilization of files. Methods: Stainless steel and nickel-titanium (NiTi) files were examined upon removal from the manufacturer’s packaging, after instrumentation in root canals of human teeth inoculated with a broth containing two anaerobic species and one facultative anaerobic species of bacteria, and after instrumentation and cleaning with either an ultrasonic bath or a thermal disinfector. For each file, the bacterial numbers were quantified using routine microbiological techniques in an anaerobic chamber. Results: No bacteria were detected from files direct from their packets. The size, taper and type of file did not affect the ability of either of the cleaning procedures to reduce bacterial numbers. However, an absence of bacteria was more likely when files were cleaned in the thermal disinfector. No bacteria were detected from files that were subjected to steam sterilization irrespective of the type of prior cleaning procedure. Conclusions: Steam sterilization eliminated all bacteria from the endodontic files irrespective of the presence of biological debris. The majority of bacteria were eliminated from endodontic files after either ultrasonic cleaning or using a thermal disinfector.; DA Van Eldik...

The microbiome of chronic rhinosinusitis: culture, molecular diagnostics and biofilm detection

Boase, S.; Foreman, A.; Cleland, E.; Tan, L.W.; Melton-Kreft, R.; Pant, H.; Hu, F.; Ehrlich, G.; Wormald, P.J.
Fonte: BioMed Central Ltd Publicador: BioMed Central Ltd
Tipo: Artigo de Revista Científica
Publicado em //2013 Português
Relevância na Pesquisa
35.88%
Background: Bacteria and fungi are believed to influence mucosal inflammation in chronic rhinosinusitis (CRS). However their presence and relationship to disease is debated. This study used multiple detection methods to compare microbial diversity and microbial abundance in healthy and diseased sinonasal mucosa. The utility of contemporary detection methods is also examined. Methods: Sinonasal mucosa was analyzed from 38 CRS and 6 controls. Bacterial and fungal analysis was performed using conventional culture, molecular diagnostics (polymerase chain reaction coupled with electrospray ionization time-of-flight mass spectrometry) and fluorescence in situ hybridization. Results: Microbes were detected in all samples, including controls, and were often polymicrobial. 33 different bacterial species were detected in CRS, 5 in control patients, with frequent recovery of anaerobes. Staphylococcus aureus and Propionibacterium acnes were the most common organisms in CRS and controls, respectively. Using a model organism, FISH had a sensitivity of 78%, and a specificity of 93%. Many species were detected in both CRS and controls however, microbial abundance was associated with disease manifestation. Conclusions: This study highlights some cornerstones of microbial variations in healthy and diseased paranasal sinuses. Whilst the healthy sinus is clearly not sterile...

Avaliação microbiológica de lesões periapicais crônicas associadas ao insucesso do retratamento endodôntico; Microbiological evaluation of chronic periapical lesions associated with unsuccessful endodontic retreatment

Fernanda Graziela Correa Signoretti
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 22/01/2013 Português
Relevância na Pesquisa
36.05%
O conhecimento do perfil microbiano envolvido na periodontite apical persistente pode auxiliar no estabelecimento de protocolos mais eficazes na conduta endodôntica. Através de um relato de caso clínico e da avaliação de 20 casos de periodontite apical persistente após retratamento endodôntico, foram objetivos deste trabalho: identificar bactérias viáveis em lesões periapicais persistentes e correlacionar os achados microbiológicos com o diagnóstico histopatológico da lesão. Métodos: No relato de caso o dente foi submetido ao retratamento endodôntico através da técnica de crown-down com o uso de substância química auxiliar (clorexidina 2% gel), patência e alargamento foraminal e obturação dos canais em sessão única. Após persistência da fístula foi indicada apicectomia, que foi realizada sob magnificação e retro-obturação com MTA. O fragmento apical da raiz distal foi observado por microscopia eletrônica de varredura e foi realizada cultura microbiana da lesão curetada (capítulo 1). Foram selecionados 20 pacientes com necessidade de cirurgia parendodôntica, submetidos à coleta durante a curetagem do tecido periapical. As amostras foram processadas microbiologicamente por técnicas de cultura microbiana e enviadas para diagnóstico histológico (capítulo 2). Resultados: No capítulo 1 as seguintes espécies foram encontradas: Actinomyces naeslundii e Actinomyces meyeri...

Microbiological Analysis of Surfaces of Leonardo Da Vinci's Atlantic Codex: Biodeterioration Risk

Tarsitani, Gianfranco; Moroni, Catia; Cappitelli, Francesca; Pasquariello, Giovanna; Maggi, Oriana
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
35.96%
Following the discovery of discoloration on some pages of the Atlantic Codex (AC) of Leonardo da Vinci kept in the Biblioteca Ambrosiana in Milan, some investigations have been carried out to verify the presence of microorganisms, such as bacteria and fungi. To verify the presence of microorganisms a noninvasive method of sampling has been used that was efficient and allowed us to highlight the microbial facies of the material that was examined using conventional microbiological techniques. The microclimatic conditions in the storage room as well as the water content of the volume were also assessed. The combined observations allowed the conclusion that the discoloration of suspected biological origin on some pages of AC is not related to the presence or current attack of microbial agents.

Detection of Salmonella sp. from porcine origin: a comparison between a PCR method and standard microbiological techniques

Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2005 Português
Relevância na Pesquisa
46.06%
The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by PCR-RVB. For the PCR-RVB method 15 Salmonella-positive samples were negative by SMT, a significant difference according to the Mac Nemar's chi-squared test (p=0.0153). Subsequent serological typing of the SMT isolates showed the following Salmonella serovars, the number of positive samples being given in parentheses: Typhimurium (12); Bredeney (10); Panama (5); Saint-paul (5); Minnesota (3); Mbandaka (2); Derby (1); Enteritidis (1); Orion (1) and Salmonella sp. (2). We concluded that...

The combined use of molecular techniques and capillary electrophoresis in food analysis

García-Cañas, Virginia; González, Ramón; Cifuentes, Alejandro
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 70846 bytes; application/pdf
Português
Relevância na Pesquisa
35.99%
We review the combined use of molecular techniques and capillary electrophoresis (CE) in food analysis. We present an up-to-date overview (including works published up to January 2004) and discuss the advantages and the drawbacks of these combined techniques. The main applications of molecular techniques in conjunction with CE in food analysis include: (i) species identification; (ii) microbiological and toxicological analysis; and (iii) detection of transgenic foods. We also outline the future outlook for this analytical methodology in food science.; Peer reviewed

Application of molecular nucleic acid-based techniques for the study of microbial communities in monuments and artworks; Aplicación de técnicas moleculares basadas en ácidos nucleicos al estudio de comunidades microbianas en monumentos y obras de arte

González Grau, Juan Miguel; Sáiz-Jiménez, Cesáreo
Fonte: Sociedad Española de Microbiología Publicador: Sociedad Española de Microbiología
Tipo: Artículo
Português
Relevância na Pesquisa
35.93%
Microorganisms play critical roles in every kind of habitat on Earth, including those constructed by humans. Thus, our cultural heritage is affected by microbial colonization. While classical microbiological methods based on culturing procedures have provided important, but limited information on the microbial diversity of natural samples, novel molecular techniques have been extremely valuable in unraveling the diversity of microbiota involved in the biodeterioration of our monuments and artworks. The knowledge gained from these approaches has allowed the design of strategies for conserving and protecting monuments for the benefit of future generations. This review describes the state-of-the-art of the application of molecular methods to the analysis of cultural assets, and provides nearfuture perspectives on the subject.; Los microorganismos desempeñan papeles críticos en todo tipo de hábitat de la Tierra, incluso en los que han sido construidos por los humanos. Así, el patrimonio cultural también se ve afectado por la colonización de microorganismos. Mientras los métodos clásicos de microbiología basados en procedimientos de cultivo han proporcionado información importante, pero limitada, de las muestras naturales...

A microbiological approach to improve the performance of single-chamber bioelectrochemical systems

Rago, Laura
Fonte: [Barcelona] : Universitat Autònoma de Barcelona, Publicador: [Barcelona] : Universitat Autònoma de Barcelona,
Tipo: Tesis i dissertacions electròniques; info:eu-repo/semantics/doctoralThesis; info:eu-repo/semantics/publishedVersion
Publicado em //2015 Português
Relevância na Pesquisa
35.96%
La necesidad de energía renovable y la constante amenaza del calentamiento global han motivado el desarrollo de nuevas tecnologías más sostenibles que las actuales, como los sistemas bioelectroquímicos, también conocidos con el nombre de celdas electroquímicas microbianas (Microbial Electrochemical Cells, MXCs), que combinan la electroquímica con el metabolismo de un grupo particular de microorganismos llamados exoelectrógenos o ARB (Anode Respiring Bacteria). Entender la actividad metabólica de los ARB y sus condiciones óptimas de crecimiento es de gran importancia para garantizar el máximo rendimiento de las MXCs. Por este motivo, la presente tesis estudia los sistemas bioelectroquímicos y tiene como objetivo desarrollar, estudiar y mejorar la comunidad microbiana de las MXCs para aumentar su rendimiento. Se han aplicado técnicas moleculares avanzadas cuales el estudio metagenómico del gen 16S ribosomal y la PCR en tiempo real (qPCR). Se han diseñado diferentes montajes experimentales para estudiar las condiciones de operación que favorecen a los ARB frente a otros microorganismos que disminuyen la eficiencia de las MXCs, como son los metanógenos o las bacterias homoacetógenas. El enriquecimiento de ARB bajo diferentes resistencias externas en celdas de combustible microbianas (Microbial Fuel Cells...

Training program on microbiological test collection material methods at a teaching hospital: investment and result assessment; Programa de capacitación sobre el método de colecta de material para análisis microbiológico en un hospital-escuela: inversión y evaluación de los resultados; Programa de treinamento sobre método de coleta de material para exame microbiológico em um hospital de ensino: investimento e avaliação dos resultados

Jericó, Marli de Carvalho; Castilho, Valéria; Perroca, Márcia Galan
Fonte: Universidade de São Paulo. Escola de Enfermagem de Ribeirão Preto Publicador: Universidade de São Paulo. Escola de Enfermagem de Ribeirão Preto
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Formato: application/pdf; application/pdf
Publicado em 01/10/2006 Português
Relevância na Pesquisa
36.05%
This study aimed at evaluating the results, direct costs and investment of a training program on microbiological test material collection at a teaching hospital. Test collections that did not follow the established criteria (failure) were considered as the result measure. Variable and absorption costing were used to calculate direct costs and investments, respectively. Of the 11,893 collected materials, failures were evidenced in 59 (0.5%). Direct cost corresponded to R$ 154.10 and R$ 2,431.29 was invested in training. These findings revealed that the evidenced number of anomalies (failures) represented a low percentage in relation to the total collected material for microbiological exams. Therefore, this should not be considered a critical point that justifies the continuity of the training and, consequently, the investment.; Este estudio tiene como objetivo evaluar los resultados, los costos directos y la inversión en un programa de capacitación para la colecta de muestras para análisis microbiológico en un hospital-escuela. Fueron consideradas como medida de resultados las colectas de análisis que no siguieron los criterios establecidos (anomalía). Los sistemas de costo variable y por absorción fueron utilizados, respectivamente...

Application of molecular nucleic acid-based techniques for the study of microbial communities in monuments and artworks

González,Juan M.; Saiz-Jiménez,Cesáreo
Fonte: International Microbiology Publicador: International Microbiology
Tipo: info:eu-repo/semantics/article; journal article; info:eu-repo/semantics/publishedVersion Formato: text/html; application/pdf
Publicado em 01/09/2005 Português
Relevância na Pesquisa
35.93%
Microorganisms play critical roles in every kind of habitat on Earth, including those constructed by humans. Thus, our cultural heritage is affected by microbial colonization. While classical microbiological methods based on culturing procedures have provided important, but limited information on the microbial diversity of natural samples, novel molecular techniques have been extremely valuable in unraveling the diversity of microbiota involved in the biodeterioration of our monuments and artworks. The knowledge gained from these approaches has allowed the design of strategies for conserving and protecting monuments for the benefit of future generations. This review describes the state-of-the-art of the application of molecular methods to the analysis of cultural assets, and provides near-future perspectives on the subject.