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Genome Sequence of Bradyrhizobium viridifuturi Strain SEMIA 690T, a Nitrogen-Fixing Symbiont of Centrosema pubescens

Helene, Luisa Caroline Ferraz; Gomes, Douglas Fabiano; Delamuta, Jakeline Renata Marçon; Ribeiro, Renan Augusto; Souza, Renata Carolini; Almeida, Luiz Gonzaga Paula; Vasconcelos, Ana Tereza Ribeiro; Hungria, Mariangela
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
SEMIA 690T is a nitrogen-fixing symbiont of Centrosema pubescens, and comprises the recently described species Bradyrhizobium viridifuturi. Its draft genome indicates that it belongs to the Bradyrhizobium elkanii superclade. SEMIA 690T carries two copies of the regulatory nodD gene, and the nod and nif operons resemble those of Bradyrhizobium diazoefficiens.

Genome Sequence of Bradyrhizobium tropiciagri Strain CNPSo 1112T, Isolated from a Root Nodule of Neonotonia wightii

Delamuta, Jakeline Renata Marçon; Gomes, Douglas Fabiano; Ribeiro, Renan Augusto; Chueire, Ligia Maria Oliveira; Souza, Renata Carolini; Almeida, Luiz Gonzaga Paula; Vasconcelos, Ana Tereza Ribeiro; Hungria, Mariangela
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
CNPSo 1112T is a nitrogen-fixing symbiont of perennial soybean, a tropical legume forage. Its draft genome indicates a large genome with a circular chromosome and 9,554 coding sequences (CDSs). Operons of nodulation, nitrogen fixation, and uptake hydrogenase were present in the symbiotic island, and the genome encompasses several CDSs of stress tolerance.

Permanent Draft Genome Sequence of Frankia sp. Strain ACN1ag, a Nitrogen-Fixing Actinobacterium Isolated from the Root Nodules of Alnus glutinosa

Swanson, Erik; Oshone, Rediet; Simpson, Stephen; Morris, Krystalynne; Abebe-Akele, Feseha; Thomas, W. Kelley; Tisa, Louis S.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Frankia strain ACN1ag is a member of Frankia lineage Ia, which are able to re-infect plants of the Betulaceae and Myricaceae families. Here, we report a 7.5-Mbp draft genome sequence with a G+C content of 72.35% and 5,687 candidate protein-encoding genes.

Complete Genome Sequence of the Porcine Epidemic Diarrhea Virus Variant CH/HNYF/2014

Li, Renfeng; Tian, Xiangqin; Qiao, Songlin; Guo, Junqing; Xie, Weitao; Zhang, Gaiping
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Sow’s milk is a potential route for the vertical transmission of porcine epidemic diarrhea virus (PEDV) from sow to suckling piglet. We report here the complete genome sequence of PEDV strain CH/HNYF/2014, which was isolated from milk samples. This information provides further understanding of the transmission mechanisms and genetic diversity of PEDV.

Draft Genome Sequence of Francisella tularensis Strain 410108 from Tibet, China

Song, Lihua; Yu, Yonghui; Feng, Le; Wang, Tao; He, Jun; Zhu, Hong; Duan, Qing
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Francisella tularensis is the etiological agent of the potentially fatal disease tularemia. Here, we report the draft genome sequence of a virulent human isolate from Tibet, China in 1962, F. tularensis strain 410108, an intermediate-genotype strain of F. tularensis subsp. holarctica between biovar japonica and non-japonica strains in the world.

Draft Genome Sequence of Caloranaerobacter sp. TR13, an Anaerobic Thermophilic Bacterium Isolated from a Deep-Sea Hydrothermal Vent

Zhou, Meixian; Xie, Yunbiao; Dong, Binbin; Liu, Qing; Chen, Xiaoyao
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Here, we report the draft 2,261,881-bp genome sequence of Caloranaerobacter sp. TR13, isolated from a deep-sea hydrothermal vent on the East Pacific Rise. The sequence will be helpful for understanding the genetic and metabolic features, as well as potential biotechnological application in the genus Caloranaerobacter.

Complete Genome Sequences of Two Listeria monocytogenes Serovars, 1/2a and 4b, Isolated from Dairy Products in Brazil

Pieta, Luiza; Campos, Fabrício Souza; Mariot, Roberta Fogliatto; Prichula, Janira; de Moura, Tiane Martin; Frazzon, Ana Paula Guedes; Frazzon, Jeverson
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Listeria monocytogenes is the foodborne pathogen responsible for a bacterial infection called listeriosis. Here, we present the whole-genome sequences of two L. monocytogenes serovars, 1/2a and 4b, which are considered the most prevalent in food processing plants and listeriosis outbreaks, respectively.

Genomic Sequence of a Clinical Vancomycin-Resistant Reference Strain, Enterococcus faecalis ATCC 51299

Sung, Kidon; Khan, Saeed; Marasa, Bernard; Min, Seonggi; Kweon, Ohgew; Nawaz, Mohamed; Cerniglia, Carl
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
In this paper, we present a draft genome sequence of a quality control reference strain, Enterococcus faecalis ATCC 51299 (multilocus sequencing type [MLST] ST6), which is sensitive to teicoplanin but resistant to vancomycin. It is used in an agar screening test for streptomycin, gentamicin, and vancomycin resistance and the resistance marker vanB.

Complete Genome Sequences of Two Shiga Toxin-Producing Escherichia coli Strains from Serotypes O119:H4 and O165:H25

Lindsey, Rebecca L.; Knipe, Kristen; Rowe, Lori; Garcia-Toledo, Lisley; Loparev, Vladimir; Juieng, Phalasy; Trees, Eija; Strockbine, Nancy; Stripling, Devon; Gerner-Smidt, Peter
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen. Here, we report complete whole-genome sequences for two STEC strains of serotypes O119:H4 and O165:H25 isolated from clinical cases in the United States.

De Novo Genome Sequence of “Candidatus Liberibacter solanacearum” from a Single Potato Psyllid in California

Wu, F.; Deng, X.; Liang, G.; Wallis, C.; Trumble, J. T.; Prager, S.; Chen, J.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Text
Publicado em 17/12/2015 Português
The draft genome sequence of “Candidatus Liberibacter solanacearum” strain RSTM from a potato psyllid (Bactericera cockerelli) in California is reported here. The RSTM strain has a genome size of 1,286,787 bp, a G+C content of 35.1%, 1,211 predicted open reading frames (ORFs), and 43 RNA genes.

N-helix and Cysteines Inter-regulate Human Mitochondrial VDAC-2 Function and Biochemistry*

Maurya, Svetlana Rajkumar; Mahalakshmi, Radhakrishnan
Fonte: American Society for Biochemistry and Molecular Biology Publicador: American Society for Biochemistry and Molecular Biology
Tipo: Text
Português
Human voltage-dependent anion channel-2 (hVDAC-2) functions primarily as the crucial anti-apoptotic protein in the outer mitochondrial membrane, and additionally as a gated bidirectional metabolite transporter. The N-terminal helix (NTH), involved in voltage sensing, bears an additional 11-residue extension (NTE) only in hVDAC-2. In this study, we assign a unique role for the NTE as influencing the chaperone-independent refolding kinetics and overall thermodynamic stability of hVDAC-2. Our electrophysiology data shows that the N-helix is crucial for channel activity, whereas NTE sensitizes this isoform to voltage gating. Additionally, hVDAC-2 possesses the highest cysteine content, possibly for regulating reactive oxygen species content. We identify interdependent contributions of the N-helix and cysteines to channel function, and the measured stability in micellar environments with differing physicochemical properties. The evolutionary demand for the NTE in the presence of cysteines clearly emerges from our biochemical and functional studies, providing insight into factors that functionally demarcate hVDAC-2 from the other VDACs.

Nitroalkenes confer acute cardioprotection via adenine nucleotide translocase 1.

Nadtochiy, Sergiy M.; Zhu, Qiuyu Martin; Urciuoli, William; Rafikov, Ruslan; Black, Stephen M.; Brookes, Paul S.
Fonte: American Society for Biochemistry and Molecular Biology Publicador: American Society for Biochemistry and Molecular Biology
Tipo: Text
Publicado em 18/12/2015 Português

Demonstration of lignin-to-peroxidase direct electron transfer. A TRANSIENT-STATE KINETICS, DIRECTED MUTAGENESIS, EPR AND NMR STUDY.

Sáez-Jiménez, Verónica; Baratto, Maria Camilla; Pogni, Rebecca; Rencoret, Jorge; Gutiérrez, Ana; Santos, José Ignacio; Martínez, Angel T.; Ruiz-Dueñas, Francisco Javier
Fonte: American Society for Biochemistry and Molecular Biology Publicador: American Society for Biochemistry and Molecular Biology
Tipo: Text
Publicado em 18/12/2015 Português

Big data meets public health: Human well-being could benefit from large-scale data if large-scale noise is minimized

Khoury, Muin J.; Ioannidis, John P. A.
Fonte: PubMed Publicador: PubMed
Tipo: Text
Publicado em 28/11/2014 Português

Quantifying the effect of pore size and surface treatment on epidermal incorporation into percutanteously implanted sphere-templated porous biomaterials in mice

Underwood, Robert A.; Usui, Marcia L.; Zhao, Ge; Hauch, Kip D.; Takeno, Marc M.; Ratner, Buddy D.; Marshall, Andrew J.; Shi, Xuefeng; Olerud, John E.; Fleckman, Philip
Fonte: PubMed Publicador: PubMed
Tipo: Text
Português
The sinus between skin and a percutaneous medical device is often a portal for infection. Epidermal integration into an optimized porous biomaterial could seal this sinus. In this study we measured epithelial ingrowth into rods of sphere-templated porous poly(2-hydroxyethyl methacrylate) [poly(HEMA)] implanted percutaneously in mice. The rods contained spherical 20, 40 or 60μm pores with and without surface modification. Epithelial migration was measured 3, 7 and 14 days post-implantation utilizing immunohistochemistry for pankeratins and image analysis. Our global results showed average keratinocyte migration distances of 81μm +/− 16.85μm (SD). Migration was shorter through 20μm pores (69.32μm +/− 21.73) compared to 40 and 60μm (87.04μm +/− 13.38 and 86.63μm +/− 8.31 respectively). Migration was unaffected by CDI surface modification without considering factors of pore size and healing duration. Epithelial integration occurred quickly showing an average migration distance of 74.13μm +/− 12.54 after 3 days without significant progression over time. These data show that the epidermis closes the sinus within 3 days, migrates into the biomaterial (an average of 11% of total rod diameter) and stops. This process forms an integrated epithelial collar without evidence of marsupialization or permigration.

Identifying Breastfeeding-Sensitive Conditions by Expert Consensus

Quinn, Celia
Fonte: PubMed Publicador: PubMed
Tipo: Text
Português

Are sirtuins viable targets for improving healthspan and lifespan?

Baur, Joseph A.; Ungvari, Zoltan; Minor, Robin K.; Couteur, David G. Le; de Cabo, Rafael
Fonte: PubMed Publicador: PubMed
Tipo: Text
Publicado em 01/06/2012 Português
Although the increased lifespan of our populations illustrates the success of modern medicine, the risk of developing many diseases increases exponentially with old age. Caloric restriction is known to retard ageing and delay functional decline as well as the onset of disease in most organisms. Studies have implicated the sirtuins (SIRT1–SIRT7) as mediators of key effects of caloric restriction during ageing. Two unrelated molecules that have been shown to increase SIRT1 activity in some settings, resveratrol and SRT1720, are excellent protectors against metabolic stress in mammals, making SIRT1 a potentially appealing target for therapeutic interventions. This Review covers the current status and controversies surrounding the potential of sirtuins as novel pharmacological targets, with a focus on SIRT1.

GATA4 represses an ileal program of gene expression in the proximal small intestine by inhibiting the acetylation of histone H3, lysine 27

Aronson, B. E.; Aronson, S. Rabello; Berkhout, R. P.; Chavoushi, S. F.; He, A.; Pu, W. T.; Verzi, M. P.; Krasinski, S. D.
Fonte: PubMed Publicador: PubMed
Tipo: Text
Português
GATA4 is expressed in the proximal 85% of small intestine where it promotes a proximal intestinal (‘jejunal’) identity while repressing a distal intestinal (‘ileal’) identity, but its molecular mechanisms are unclear. Here, we tested the hypothesis that GATA4 promotes a jejunal vs. ileal identity in mouse intestine by directly activating and repressing specific subsets of absorptive enterocyte genes by modulating the acetylation of histone H3, lysine 27 (H3K27), a mark of active chromatin, at sites of GATA4 occupancy. Global analysis of mouse jejunal epithelium showed a statistically significant association of GATA4 occupancy with GATA4-regulated genes. Occupancy was equally distributed between down- and up-regulated targets, and occupancy sites showed a dichotomy of unique motif over-representation at down- vs. up-regulated genes. H3K27ac enrichment at GATA4-binding loci that mapped to down-regulated genes (activation targets) was elevated, changed little upon conditional Gata4 deletion, and was similar to control ileum, whereas H3K27ac enrichment at GATA4-binding loci that mapped to up-regulated genes (repression targets) was depleted, increased upon conditional Gata4 deletion, and approached H3K27ac enrichment in wildtype control ileum. These data support the hypothesis that GATA4 both activates and represses intestinal genes...

NRF2, cancer and calorie restriction

Martín-Montalvo, A; Villalba, JM; Navas, P; de Cabo, R
Fonte: PubMed Publicador: PubMed
Tipo: Text
Português
The transcription factor NF-E2-related factor (NRF2) is a key regulator of several enzymatic pathways, including cytoprotective enzymes in highly metabolic organs. In this review, we summarize the ongoing research related to NRF2 activity in cancer development, focusing on in vivo studies using NRF2 knockout (KO) mice, which have helped in defining the crucial role of NRF2 in chemoprevention. The lower cancer protection observed in NRF2 KO mice under calorie restriction (CR) suggests that most of the beneficial effects of CR on the carcinogenesis process are likely mediated by NRF2. We propose that future interventions in cancer treatment would be carried out through the activation of NRF2 in somatic cells, which will lead to a delay or prevention of the onset of some forms of human cancers, and subsequently an extension of health- and lifespan.

Attitudes, Knowledge, and Correlates of Self-Efficacy for the Provision of Safer Conception Counseling Among Ugandan HIV Providers

Goggin, Kathy; Finocchario-Kessler, Sarah; Staggs, Vincent; Woldetsadik, Mahlet Atakilt; Wanyenze, Rhoda K.; Beyeza-Kashesya, Jolly; Mindry, Deborah; Khanakwa, Sarah; Wagner, Glenn J.
Fonte: Mary Ann Liebert, Inc. Publicador: Mary Ann Liebert, Inc.
Tipo: Text
Publicado em 01/12/2015 Português
High rates of childbearing desires (59%) and serodiscordant partnerships (50%) among people living with HIV (PHLA) in Uganda highlight the need for safer conception counseling (SCC). Provider attitudes about counseling PLHA on the use of safer conception methods (SCM) have been explored in qualitative studies, but published quantitative investigations are scarce. Data from 57 Ugandan providers were collected to examine providers' attitudes about childbearing among PLHA and engagement in discussions about childbearing, as well as their knowledge, interest, self-efficacy, and intentions to provide SCC. Correlates of self-efficacy for the provision of SCC were explored to inform the development of training programs. Providers reported a general awareness of most SCM, especially timed unprotected intercourse (TUI); but just over half felt they knew enough to counsel clients in the future and all wanted more training. Childbearing was discussed with less than a third of reproductive aged patients and was mostly initiated by patients. Most providers saw value in providing SCC and believed that most aspects of SCM would be acceptable to their clients, but numerous barriers were endorsed. Self-efficacy was greatest among providers who had had more childbearing conversations...