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PubMed é um banco de dados que possibilita a pesquisa bibliográfica em mais de 17 milhões de referências de artigos médicos publicados em cerca de 3.800 revistas científicas .

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The influenza A virus spliced messenger RNA M mRNA3 is not required for viral replication in tissue culture

Jackson, David; Lamb, Robert A.
Fonte: PubMed Publicador: PubMed
Tipo: Text
Publicado em /12/2008 Português
Influenza A virus genome RNA segment 7 encodes three known mRNAs, two of which, M2 mRNA and M mRNA3, are derived by alternative splicing of the primary collinear mRNA transcript using alternative 5′ splice sites. The function of M mRNA3 is currently unknown, therefore we attempted to determine whether it is essential for virus replication. Recombinant viruses unable to produce M mRNA3 and/or M2 mRNA were created by mutating the shared 3′ splice site. Growth of the mutant viruses in M2-expressing MDCK cells was not significantly affected by the lack of M mRNA3. During the course of a wild-type virus infection, levels of M mRNA3 began to decrease while those of M2 mRNA increased, which may indicate a potential mechanism of alternative splicing control. These data suggest that neither M mRNA3 nor any potential protein product are essential for influenza virus replication in tissue culture.

Towards Mobile OCR: How To Take a Good Picture of a Document Without Sight

Cutter, Michael; Manduchi, Roberto
Fonte: PubMed Publicador: PubMed
Tipo: Text
Publicado em //2015 Português
The advent of mobile OCR (optical character recognition) applications on regular smartphones holds great promise for enabling blind people to access printed information. Unfortunately, these systems suffer from a problem: in order for OCR output to be meaningful, a well-framed image of the document needs to be taken, something that is difficult to do without sight. This contribution presents an experimental investigation of how blind people position and orient a camera phone while acquiring document images. We developed experimental software to investigate if verbal guidance aids in the acquisition of OCR-readable images without sight. We report on our participant's feedback and performance before and after assistance from our software.

On the Conceptual Ambiguity Surrounding Perceived Message Effectiveness

Yzer, Marco; LoRusso, Susan; Nagler, Rebekah H.
Fonte: PubMed Publicador: PubMed
Tipo: Text
Publicado em //2015 Português
Health message quality is best understood in terms of a message’s ability to effectively produce change in the variables that it was designed to change. The importance of determining a message’s effectiveness in producing change prior to implementation is clear: The better a message’s potential effectiveness is understood, the better able interventionists are to distinguish effective from ineffective messages before allocating scarce resources to message implementation. For this purpose, research has relied on perceived message effectiveness measures as a proxy of a message’s potential effectiveness. Remarkably, however, very little conceptual work has been done on perceived message effectiveness, which renders its measures under-informed and inconsistent across studies. To encourage greater conceptual work on this important construct, we review several threats to the validity of existing measures and consider strategies for improving our understanding of perceived message effectiveness.

Development of an endoluminal high-intensity ultrasound applicator for image-guided thermal therapy of pancreatic tumors

Adams, Matthew S.; Scott, Serena J.; Salgaonkar, Vasant A.; Jones, Peter D.; Plata-Camargo, Juan C.; Sommer, Graham; Diederich, Chris J.
Fonte: PubMed Publicador: PubMed
Tipo: Text
Português
An ultrasound applicator for endoluminal thermal therapy of pancreatic tumors has been introduced and evaluated through acoustic/biothermal simulations and ex vivo experimental investigations. Endoluminal therapeutic ultrasound constitutes a minimally invinvasive conformal therapy and is compatible with ultrasound or MR-based image guidance. The applicator would be placed in the stomach or duodenal lumen, and sonication would be performed through the luminal wall into the tumor, with concurrent water cooling of the wall tissue to prevent its thermal injury. A finite-element (FEM) 3D acoustic and biothermal model was implemented for theoretical analysis of the approach. Parametric studies over transducer geometries and frequencies revealed that operating frequencies within 1-3 MHz maximize penetration depth and lesion volume while sparing damage to the luminal wall. Patient-specific FEM models of pancreatic head tumors were generated and used to assess the feasibility of performing endoluminal ultrasound thermal ablation and hyperthermia of pancreatic tumors. Results indicated over 80% of the volume of small tumors (~2 cm diameter) within 35 mm of the duodenum could be safely ablated in under 30 minutes or elevated to hyperthermic temperatures at steady-state. Approximately 60% of a large tumor (~5 cm diameter) model could be safely ablated by considering multiple positions of the applicator along the length of the duodenum to increase coverage. Prototype applicators containing two 3.2 MHz planar transducers were fabricated and evaluated in ex vivo porcine carcass heating experiments under MR temperature imaging (MRTI) guidance. The applicator was positioned in the stomach adjacent to the pancreas...

TEMEL ETİK KURAMLAR AÇISINDAN ADALET ve SAĞLIK HAKKI KAVRAMLARININ DEĞERLENDİRMESİ1,2

Ekmekçi, Perihan Elif; Arda, Berna
Fonte: PubMed Publicador: PubMed
Tipo: Text
Português
Sağlık hakkı temel bir insan hakkıdır. Ancak günümüzde sağlık hakkının hayata geçmesi, sağlık sunumunda gereksinim duyulan kaynakların kısıtlı olması nedeniyle engeller ile karşılaşmaktadır. Ayrıca tıp alanındaki hızlı teknolojik ilerlemeler, tanı ve tedavi imkânlarının her geçen gün gelişmesine yol açmaktadır. Bu durum, sağlık hizmetlerinin sunumunu dağıtıcı adaletin bir süjesi haline getirmektedir. Adalet kavramının tanımlanabilmesi için öncelikle, birey ya da toplulukların bir şeye ilişkin hak talebinde bulunmalarının koşullarını tanımlanmalı daha sonra hak edilenin kim tarafından ve nasıl verileceğinin belirlenmelidir. Etik kuramlar, etik açıdan doğru eyleyebilmek için hangi dayanak noktalarından yola çıkarak hangi değerleri önceleyerek karar vermek gerektiği konusunda kendi paradigmalarını oluşturmuşlardır. Adalet ve sağlık hakkı gibi temel kavramlar, temel etik kuramlar tarafından, o kuramın bağlamı içinde değerlendirilmekte ve anlam kazanmaktadır. Sağlık hakkı bazı etik kuramlar tarafından insan varlığının doğal bir bileşeni olarak tanımlanırken, bazı etik kuramlar tarafından bağlamsal olarak kabul edilmekte bazıları tarafından ise reddedilmekte...

Pulsed Focused Ultrasound Induced Displacements in Confined In Vitro Blood Clots

Wright, Cameron C.; Hynynen, Kullervo; Goertz, David E.
Fonte: PubMed Publicador: PubMed
Tipo: Text
Português
Ultrasound has been shown to potentiate the effects of tissue plasminogen activator (tPA) to improve clot lysis in a range of in vitro and in vivo studies as well as in clinical trials. One possible mechanism of action is acoustic radiation force induced clot displacements. In this study we investigate the temporal and spatial dynamics of clot displacements and strain initiated by focused ultrasound pulses. Displacements were produced by a 1.51 MHz f-number 1 transducer over a range of acoustic powers (1–85 W) in clots constrained within an agar vessel phantom channel. Displacements were tracked during and after a 5.45 ms therapy pulse using a 20 MHz high frequency ultrasound imaging probe. Peak thrombus displacements were found to be linear as a function of acoustic power up to 60 W before leveling off near 128 μm for the highest transmit powers. The time to peak displacement and recovery time of blood clots were largely independent of acoustic powers with measured values near 2 ms. A linear relationship between peak axial strain and transmit power was observed, reaching a peak value of 11% at 35 W. The peak strain occurred ~0.75 mm from the focal zone for all powers investigated in both lateral and axial directions. These results indicate that substantial displacements can be induced by focused ultrasound in confined blood clots...

Airborne measurements of organosulfates over the continental U.S.

Liao, Jin; Froyd, Karl D; Murphy, Daniel M; Keutsch, Frank N; Yu, Ge; Wennberg, Paul O; St Clair, Jason M; Crounse, John D; Wisthaler, Armin; Mikoviny, Tomas; Jimenez, Jose L; Campuzano-Jost, Pedro; Day, Douglas A; Hu, Weiwei; Ryerson, Thomas B; Pollack,
Fonte: John Wiley & Sons, Ltd Publicador: John Wiley & Sons, Ltd
Tipo: Text
Português
Organosulfates are important secondary organic aerosol (SOA) components and good tracers for aerosol heterogeneous reactions. However, the knowledge of their spatial distribution, formation conditions, and environmental impact is limited. In this study, we report two organosulfates, an isoprene-derived isoprene epoxydiols (IEPOX) (2,3-epoxy-2-methyl-1,4-butanediol) sulfate and a glycolic acid (GA) sulfate, measured using the NOAA Particle Analysis Laser Mass Spectrometer (PALMS) on board the NASA DC8 aircraft over the continental U.S. during the Deep Convective Clouds and Chemistry Experiment (DC3) and the Studies of Emissions and Atmospheric Composition, Clouds, and Climate Coupling by Regional Surveys (SEAC4RS). During these campaigns, IEPOX sulfate was estimated to account for 1.4% of submicron aerosol mass (or 2.2% of organic aerosol mass) on average near the ground in the southeast U.S., with lower concentrations in the western U.S. (0.2–0.4%) and at high altitudes (<0.2%). Compared to IEPOX sulfate, GA sulfate was more uniformly distributed, accounting for about 0.5% aerosol mass on average, and may be more abundant globally. A number of other organosulfates were detected; none were as abundant as these two. Ambient measurements confirmed that IEPOX sulfate is formed from isoprene oxidation and is a tracer for isoprene SOA formation. The organic precursors of GA sulfate may include glycolic acid and likely have both biogenic and anthropogenic sources. Higher aerosol acidity as measured by PALMS and relative humidity tend to promote IEPOX sulfate formation...

An Intracellular Laccase Is Responsible for Epicatechin-Mediated Anthocyanin Degradation in Litchi Fruit Pericarp1[OPEN]

Fang, Fang; Zhang, Xue-lian; Luo, Hong-hui; Zhou, Jia-jian; Gong, Yi-hui; Li, Wen-jun; Shi, Zhao-wan; He, Quan; Wu, Qing; Li, Lu; Jiang, Lin-lin; Cai, Zhi-gao; Oren-Shamir, Michal; Zhang, Zhao-qi; Pang, Xue-qun
Fonte: American Society of Plant Biologists Publicador: American Society of Plant Biologists
Tipo: Text
Português
Anthocyanin degradation by an intracellular laccase, mediated by epicatechin oxidation, causes the loss of red color in Lichi fruit peel.

RNA Silencing of Exocyst Genes in the Stigma Impairs the Acceptance of Compatible Pollen in Arabidopsis1[OPEN]

Safavian, Darya; Zayed, Yara; Indriolo, Emily; Chapman, Laura; Ahmed, Abdalla; Goring, Daphne R.
Fonte: American Society of Plant Biologists Publicador: American Society of Plant Biologists
Tipo: Text
Português
Stigma-specific RNA silencing of exocyst genes impairs basal pollen responses in the stigma following compatible pollinations.

Evolution of Cis-Regulatory Elements and Regulatory Networks in Duplicated Genes of Arabidopsis1[OPEN]

Arsovski, Andrej A.; Pradinuk, Julian; Guo, Xu Qiu; Wang, Sishuo; Adams, Keith L.
Fonte: American Society of Plant Biologists Publicador: American Society of Plant Biologists
Tipo: Text
Português
Cis-regulatory elements and regulatory networks have diverged considerably in duplicated genes.

A Physiological and Behavioral Mechanism for Leaf Herbivore-Induced Systemic Root Resistance1[OPEN]

Erb, Matthias; Robert, Christelle A.M.; Marti, Guillaume; Lu, Jing; Doyen, Gwladys R.; Villard, Neil; Barrière, Yves; French, B. Wade; Wolfender, Jean-Luc; Turlings, Ted C.J.; Gershenzon, Jonathan
Fonte: American Society of Plant Biologists Publicador: American Society of Plant Biologists
Tipo: Text
Português
Leaf herbivore attack changes free and conjugated phenolic acids in maize roots and thereby triggers an avoidance response in a specialist root herbivore.

FlexFlux: combining metabolic flux and regulatory network analyses

Marmiesse, Lucas; Peyraud, Rémi; Cottret, Ludovic
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Text
Publicado em 15/12/2015 Português

Evaluation of acute effect of light-emitting diode (LED) phototherapy on muscle deoxygenation and pulmonary oxygen uptake kinetics in patients with diabetes mellitus: study protocol for a randomized controlled trial

Francisco, Cristina de Oliveira; Beltrame, Thomas; Ferraresi, Cleber; Parizotto, Nivaldo Antonio; Bagnato, Vanderlei Salvador; Borghi Silva, Audrey; Benze, Benedito Galvão; Porta, Alberto; Catai, Aparecida Maria
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Text
Publicado em 15/12/2015 Português

Risk factors associated with abscess formation among patient with leg erysipelas (cellulitis) in sub-Saharan Africa: a multicenter study

Pitché, Palokinam Vincent; Saka, Bayaki; Diatta, Ahy Boubacar; Faye, Ousmane; Diané, Boh Fanta; Sangaré, Abdoulaye; Niamba, Pascal; Mandengue, Christine; Kobengue, Léon; Diop, Assane; Ly, Fatimata; Dieng, Mame Thierno; Dicko, Alassane; Soumah, Maciré
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Text
Publicado em 15/12/2015 Português

No evidence for viral sequences in five lepidic adenocarcinomas (former “BAC”) by a high-throughput sequencing approach

Berthet, Nicolas; Frangeul, Lionel; Olaussen, Ken André; Brambilla, Elisabeth; Dorvault, Nicolas; Girard, Philippe; Validire, Pierre; Fadel, Elie; Bouchier, Christiane; Gessain, Antoine; Soria, Jean-Charles
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Text
Publicado em 15/12/2015 Português

Tasquinimod triggers an early change in the polarization of tumor associated macrophages in the tumor microenvironment

Olsson, Anders; Nakhlé, Jessica; Sundstedt, Anette; Plas, Pascale; Bauchet, Anne-Laure; Pierron, Valérie; Bruetschy, Luce; Deronic, Adnan; Törngren, Marie; Liberg, David; Schmidlin, Fabien; Leanderson, Tomas
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Text
Publicado em 15/12/2015 Português

Designed ankyrin repeat proteins are effective targeting elements for chimeric antigen receptors

Hammill, Joanne A.; VanSeggelen, Heather; Helsen, Christopher W.; Denisova, Galina F.; Evelegh, Carole; Tantalo, Daniela G. M.; Bassett, Jennifer D.; Bramson, Jonathan L.
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Text
Publicado em 15/12/2015 Português

Quantitative proteomics identifies 38 proteins that are differentially expressed in cucumber in response to cucumber green mottle mosaic virus infection

Liu, Hua-Wei; Liang, Chao-Qiong; Liu, Peng-Fei; Luo, Lai-Xin.; Li, Jian-Qiang
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Text
Publicado em 15/12/2015 Português

Ashwagandha attenuates TNF-α- and LPS-induced NF-κB activation and CCL2 and CCL5 gene expression in NRK-52E cells

Grunz-Borgmann, Elizabeth; Mossine, Valeri; Fritsche, Kevin; Parrish, Alan R.
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Text
Publicado em 15/12/2015 Português

Shared Decision Making in mental health care using Routine Outcome Monitoring as a source of information: a cluster randomised controlled trial

Metz, Margot J.; Franx, Gerdien C.; Veerbeek, Marjolein A.; de Beurs, Edwin; van der Feltz-Cornelis, Christina M.; Beekman, Aartjan T. F.
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Text
Publicado em 15/12/2015 Português