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Estudo do efeito protector da folha e fruto da espécie Arbutus unedo L. na danificação oxidativa em eritrócitos humanos

Mendes, Lídia Isaura Sousa
Fonte: [s.n.] Publicador: [s.n.]
Tipo: Dissertação de Mestrado
Publicado em //2010 Português
Relevância na Pesquisa
72.05895%
Trabalho apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas; O Arbutus unedo L., vulgarmente designado por medronheiro, é uma planta endémica da região Mediterrânica amplamente utilizada na alimentação e na medicina tradicional devido às suas actividades biológicas. Estudos fitoquímicos realizados nesta espécie evidenciaram que os extractos da folha e fruto contêm diversos antioxidantes naturais. As folhas apresentam na sua constituição compostos fenólicos, tais como taninos, flavonóides e glicosídeos fenólicos e α-tocoferol, enquanto os frutos possuem também variados compostos fenólicos (antocianinas, derivados do ácido gálhico, taninos e flavonóides), vitamina C e E e carotenos. Assim, é de prever que a espécie Arbutus unedo L. tenha um considerável potencial antioxidante. No entanto, as propriedades antioxidantes desta espécie em membranas biológicas não foram até então avaliadas. Por esta razão, o presente trabalho deteve-se na avaliação do efeito protector dos extractos aquosos da folha e fruto de Arbutus unedo L. na danificação oxidativa induzida por radicais livres, utilizando como modelo celular in vitro o eritrócito humano. O 2...

Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals

Oliveira, Ivo; Coelho, Valentim; Baltasar, R.; Pereira, J.A.; Baptista, Paula
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
101.0208%
Despite strawberry tree (Arbutus unedo L.) leaves had a long use in traditional medicine due to its antiseptic, diuretic, astringent and depurative properties, the potential of their antioxidant activity are still lacking. Our study goals to assess the antioxidant and free radical scavenging potential of water, ethanol, methanol and diethyl ether extracts of A. unedo leaves. Total phenols content was achieved spectrophotometrically using Folin–Ciocalteau reagent with gallic acid as standard. Antioxidant activity was evaluated using three different methods: reducing power of iron (III)/ferricyanide complex assay, scavenging effect on DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and scavenging effect on superoxide radicals by using the PMS–NADH–nitroblue tetrazolium system. Ethanol extracts of A. unedo leaves were the highest in reducing power (IC50 232.7 lg/mL) and DPPH scavenging effect (IC50 63.2 lg/mL) followed by water extracts (with IC50 of 287.7 and 73.7 lg/mL, respectively); whereas diethyl ether extracts were the lowest. In the scavenging on superoxide radical assay, methanol extracts obtained the best results (IC50 6.9 lg/mL). For all the methods tested the antioxidant activity was concentration dependent. In accordance with antioxidant activity...

Caracterização morfológica, molecular e quimica de Arbutus unedo L. com vista à selecção de genótipos de superior qualidade

Sá, Olga
Fonte: Instituto Politécnico de Bragança, Escola Superior Agrária Publicador: Instituto Politécnico de Bragança, Escola Superior Agrária
Tipo: Dissertação de Mestrado
Português
Relevância na Pesquisa
101.5259%
O medronheiro (Arbutus unedo L.) é uma árvore fruteira de elevado valor ornamental, ambiental, económico e medicinal devido às propriedades atribuídas às flores, frutos e folhas. No entanto, nos últimos anos não tem sido atribuída a devida importância a esta espécie na região de Trás-os-Montes, ao contrário do que se verifica no resto do país, registando-se um declínio da área ocupada por A. unedo nesta região. Com o objectivo de preservar e potenciar a produção desta espécie procedeu-se, numa primeira fase, à avaliação da diversidade de 4 populações naturais de A. unedo do interior Norte e Centro de Portugal, através da caracterização morfológica e molecular. Numa segunda fase, avaliaram-se as propriedades químicas de 19 genótipos provenientes da região de Bragança, com vista a seleccionar os de superior qualidade. Desta forma espera-se contribuir para a multiplicação e valorização de populações de maior interesse. A diversidade de populações naturais de A. unedo foi avaliada através da análise morfológica e genética pela utilização de marcadores moleculares, num total de 46 genótipos. Para tal, foram colhidas 40 folhas aleatoriamente em cada árvore, nas quais se mediu o comprimento...

Analysis of molecular and morphological diversity of Arbutus unedo in the interior North and Center of Portugal.

Lopes, Lucas; Sá, Olga; Baltasar, José Luís; Pereira, J.A.; Baptista, Paula
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Português
Relevância na Pesquisa
80.736504%
In the Mediterranean Basin, the strawberry tree (Arbutus unedo L.) has medicinal, ornamental, economical, and environmental importance. During the last decades, several occurrences have caused the decline of the strawberry tree in Portugal being replaced by other species. In this context, the knowledge of genetic variability in the wild populations is essential for a proper conservation. This work aimed to characterize genetically and morphological 38 A. unedo genotypes, 30 of which located in Trás-os-Montes e Alto Douro region, and the remaining 8 genotypes in Beira Interior region, to assess their biodiversity. The genetic diversity was assessing by RAPD (Random Amplification of Polymorphic DNA) markers. The dendrogram was obtained from the matrix of pairwise distances through the Nei and Li coefficient using the UPGMA (Unweighted Pair Group Method with Arithmetic Mean) grouping method. Morphological characterization was performed through the evaluation of several quantitative traits measured on 40 fresh leaves per plant. The quantitative traits analysed were leave length and width, leave length/width ratio, peduncle length and leave fresh and dry weigh. Distance matrix of morphologic data was assessed using Euclidean distance. A Mantel test was used to analyze correlations between genetic...

Caracterização de Arbutus unedo L.: estudo da maturação dos frutos na composição química e atividade antioxidante

Alves, Maria João Teixeira
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Dissertação de Mestrado
Português
Relevância na Pesquisa
101.31838%
A espécie Arbutus unedo L., vulgarmente conhecida como medronheiro, é uma árvore de pequenas dimensões que surge dispersa em todo o território nacional. Vários usos tradicionais, sobretudo no âmbito medicinal e alimentar, têm sido citados para os seus frutos (medronho), folhas e raízes. Contudo, a sua potencialidade neste âmbito não tem sido devidamente explorado, o que tem contribuído para a redução da área ocupada por esta espécie nos povoamentos florestais. O presente trabalho teve como principal objetivo complementar os conhecimentos sobre os usos tradicionais do medronheiro, pela exploração do seu potencial nutricional e nutracêutico, de maneira a utilizá-los em processos de desenvolvimento tecnológico valorizando-se, desta forma, a espécie. Para tal, foi avaliada a atividade antioxidante das folhas, frutos e, pela primeira vez, das cascas e das flores do medronheiro. Nesta análise recorreu-se a três ensaios: efeito bloqueador dos radicais livres 2,2-Difenil-1-picrihidrazilo (DPPH), poder redutor e capacidade redutora total. Nos frutos avaliou-se, adicionalmente, a sua cor e a composição nutricional e nutracêutica, bem como a influência do estado de maturação nestes parâmetros. A análise nutricional incluiu a avaliação da humidade...

Arbutus unedo L. and its benefits on human health

Oliveira, Ivo; Baptista, Paula; Bento, Albino; Pereira, J.A.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
80.833296%
Arbutus unedo has been long used in folk medicine, throughout Mediterranean countries, with the employment of infusions and decoctions of almost all parts of this plant: leaves, fruits, barks and roots. The application of these traditional remedies arises from several health-promoting characteristics, for treatment of gastrointestinal and urological problems, hypertension and cardiac diseases, diabetes and as anti-inflammatory agent, among other interesting properties. Antioxidant ability of A. unedo shrub is also known, and antimicrobial activity has also been reported. Several compounds present in different parts of the plant may be linked to these properties. Included in those are carotenoids, flavonoids, phenolic acids and vitamins (C and E). Other bioactive compounds may be also found in different parts of A. unedo, like terpenoids and organic acids. This review will focus on the known composition of several parts of A. unedo, their antioxidant ability and traditional use, and the available data sustaining the rationality of the use as part of folk medicine.

Volatile profile of Arbutus unedo L. fruit through ripening stage

Oliveira, Ivo; Pinho, P. Guedes de; Malheiro, R.; Baptista, Paula; Pereira, J.A.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
101.0208%
Arbutus unedo L. is a Mediterranean shrub used in the production of jams, marmalades and beverages, as well as in traditional medicine. In this work, the effect of ripening on the volatile compounds of the fruits of A. unedo was evaluated, using headspace-solid phase microextraction combined with gas chromatography/ ion trap-mass spectrometry. Alcohols are the main component of the volatile fraction of A. unedo fruits, followed by aldehydes and esters. The main volatile compounds identified were (Z)-3-hexen-1-ol, 1-hexanol, hexanal, (E)-2-hexenal and hexyl acetate, all formed through the lipoxygenase pathway (LOX). The green odours are progressively replaced during ripening by floral and sweet sensations, due to the decrease in alcohols, aldehydes and esters, resulting in the unmasking of the aromas associated with the minor compounds (mainly monoterpenes and norisoprenoid compounds) that compose A. unedo fruits.

Influence of strawberry tree (Arbutus unedo L.) fruit ripening stage on chemical composition and antioxidant activity

Oliveira, Ivo; Baptista, Paula; Malheiro, R.; Casal, Susana; Bento, Albino; Pereira, J.A.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
90.527705%
Arbutus unedo is a widespread shrub with economic importance, derived from the use of its berries in the production of alcoholic beverages and in folk medicine. This work intends to evaluate for the first time the effect of fruit ripening stage on antioxidant activity, total phenolic content, fatty acid profile and tocopherol composition. Ripe fruits shown higher extraction yield (45.04±2.23%) when compared to other fruit ripening stages. By contrast, total phenol contents were higher in the unripe and intermediate stage of ripeness (108 and 111 mg GAES/g dry fruit, respectively, against 60 mg/g dry fruit when ripe). Ripe and intermediate fruits shown the lower EC50 values on the DPPH radicals (0.25±0.02 mg/mL) and reducing power assay (1.09± 0.05 mg/mL), respectively. A significant correlation was established between antioxidant activity and fruits ripening stage. Fatty acid profiles were very similar between the ripening stages, being alfa-linolenic, linoleic and oleic, the three major ones. Polyunsaturated fatty acids (PUFA) represent as much as 60% of the total fatty acids, with a highly favorable omega 3/omega 6 ratio. From the analysis of the vitamin E vitamers, the most important was γ-tocotrienol, with a clear reduction in the total free vitamin E content with ripening. These results highlight that the fruits of intermediate ripeness can be regarded important sources of biologically active compounds with a fatty acid profile rich in omega-3 PUFA...

Optimization of DNA extraction for RAPD and ISSR analysis of Arbutus unedo L. leaves

Sá, Olga; Pereira, J.A.; Baptista, Paula
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
90.59216%
Genetic analysis of plants relies on high yields of pure DNA. For the strawberry tree (Arbutus unedo) this represents a great challenge since leaves can accumulate large amounts of polysaccharides, polyphenols and secondary metabolites, which co-purify with DNA. For this specie, standard protocols do not produce efficient yields of high-quality amplifiable DNA. Here, we present for the first time an improved leaf-tissue protocol, based on the standard cetyl trimethyl ammonium bromide protocol, which yields large amounts of high-quality amplifiable DNA. Key steps in the optimized protocol are the addition of antioxidant compounds—namely polyvinyl pyrrolidone (PVP), 1,4-dithiothreitol (DTT) and 2-mercaptoethanol, in the extraction buffer; the increasing of CTAB (3%, w/v) and sodium chloride (2M) concentration; and an extraction with organic solvents (phenol and chloroform) with the incubation of samples on ice. Increasing the temperature for cell lyses to 70 °C also improved both DNA quality and yield. The yield of DNA extracted was 200.0 ± 78.0 μg/μL and the purity, evaluated by the ratio A260/A280, was 1.80 ± 0.021, indicative of minimal levels of contaminating metabolites. The quality of the DNA isolated was confirmed by random amplification polymorphism DNA and by inter-simple sequence repeat amplification...

Comparative antihemolytic and radical scavenging activities of strawberry tree (Arbutus unedo L.) leaf and fruit

Mendes, Lídia; de Freitas, Victor; Baptista, Paula; Carvalho, M.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
91.09398%
The present study reports the antioxidant properties of Arbutus unedo L. leaf and fruit extracts using different in vitro assays including (i) reducing power, (ii) scavenging effect on DPPH free radicals, and (iii) inhibitory effect on AAPH-induced hemolysis and lipid peroxidation in human erythrocytes. All assays demonstrated antioxidant efficiency for A. unedo L. aqueous extracts, being consistently higher in the leaf. EC50 values for reducing power and DPPH radical scavenging activities were, respectively, 0.318 ± 0.007 and 0.087 ± 0.007 mg/mL for leaf, and 2.894 ± 0.049 and 0.790 ± 0.016 mg/mL for fruit extracts. Under the oxidative action of AAPH, A. unedo leaf and fruit extracts protected the erythrocyte membrane from hemolysis (IC50 of 0.062 ± 0.002 and 0.430 ± 0.091 mg/mL, respectively) and decreased the levels of malondialdehyde, a breakdown product of lipid peroxidation (IC50 of 0.075 ± 0.014 and 0.732 ± 0.452 mg/mL, respectively). In accordance with antioxidant activity, phenolic content was found to be significantly higher in leaf extract. To our knowledge, this is the first time that the antioxidant activity of A. unedo species is evaluated using human biological membranes. Overall, our results suggest that A. unedo leaves are a promising source of natural antioxidants with potential application in diseases mediated by free radicals.

Arbutus unedo L. leaves as source of phytochemicals with bioactive properties

Malheiro, R.; Sá, Olga; Pereira, Eric Carvalho; Aguiar, Cristina; Baptista, Paula; Pereira, J.A.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
101.0208%
In recent years the strawberry tree (Arbutus unedo L.) is being gradually replaced by other species with higher economic value. With the ultimate goal of selecting superior genotypes, the present work was initiated to study the antioxidant and antimicrobial activities, and total phenolic content in 19 different genotypes of A. unedo leaves from the Trás-os-Montes region of Portugal. The genotype Braganc¸ a 1 contains higher total phenolic content (215.0 mg GAE/gextract) whereas the Vila Boa 4 genotype shows lower total phenolic content (148.0 mg GAE/gextract). In both methods tested to evaluate the antioxidant activity, Vila Verde and Donai displayed the highest antioxidant capacity (EC50 values of 0.088 and 0.090 mg/mL, respectively, for DPPH; EC50 values of 0.233 and 0.245 mg/mL, respectively, for reducing power assay) while Vila Boa 2 reported the lowest antioxidant potential (EC50 values of 0.142 and 0.378 mg/mL, respectively, in DPPH and reducing power methods). Linear negative correlations were established between the total phenol contents and the EC50 values for both of the antioxidant activity methods tested. Preliminary assays for antimicrobial potential showed that extracts from A. unedo leaves display antibacterial activity...

Genetic diversity of Portuguese Arbutus unedo L. populations using leaf traits and molecular markers: an approach for conservation purposes

Lopes, Lucas; Sá, Olga; Pereira, J.A.; Baptista, Paula
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
100.59216%
The genetic diversity within and between four natural Arbutus unedo L. populations in Portugal was investigated using morphological characters, inter-simple sequence repeats (ISSR) and random amplified polymorphic (RAPD) markers, for a proper conservation of this resource. The results showed a high morphological variation between and within-populations. The dry-weight, length, width and peduncle length of the leaves were the most discriminating characters, being responsible for 52.6% of the total variation. The molecular analysis, based on both RAPD and ISSR, indicated moderate genetic diversity within populations, with values of Nei’s gene diversity ranging from 0.16 to 0.39 and the Shannon’s diversity from 0.24 to 0.57. The highest genetic diversity occurred within Braganc¸ a population. At the species level, the genetic variation was mainly within populations (75%), suggesting a low-moderate genetic differentiation among populations. The high level of gene flow estimated among populations (1.499) showed to be the main reason for their low differentiation. The cluster analysis showed that only one population formed a distinctive cluster. The remaining formed a second cluster due to their geographical approach, which has been showed to affect significantly the genetic distances among populations. No correlation between RAPD and ISSR markers...

Mycorrhizal synthesis between Pisolithus arhizus and adult clones of Arbutus unedo in vitro and in nursery

Gomes, Filomena; Machado, Helena; San Martin, Esteban; Portugal, A.; Canhoto, J. M.
Fonte: Northeast Forestry University and Springer-Verlag Publicador: Northeast Forestry University and Springer-Verlag
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
80.736504%
Arbutoid mycorrhizae were synthesized between adult selected clones of Arbutus unedo L. and Pisolithus arhizus. Two micropropagated clones were tested: AL1, in vitro and C1 (acclimatized plants) in nursery and later in a field trial. In vitro, rooted shoots were transferred to test tubes containing the substrate previously inoculated with mycelium cultured on agar. In the nursery, two inoculation treatments were tested (vegetative inocula or dry sporocarps) and compared to control plants. In the field trial, plants from nursery inoculation treatments were compared and an additional control treatment using seedlings was implemented. Plant height was evaluated 4 months later in the nursery and 20 months later in the field trial. Roots were examined by morphological and histological studies: a) in vitro plantlets one month after inoculation and nine months after acclimatization; and b) 20 months after the field trial was established. Arbutoid mycorrhizae were observed in vitro one month after inoculation, indicating compatibility between A. unedo and P. arhizus. These showed the presence of a mantle, Hartig net, and intracellular hyphal complexes confined to the epidermal root cells. Arbutoid mycorrhizae were also observed nine months after acclimatiza- Fund project: This work was supported by a PhD fellowship (SFRH/BD/37170/2007) from the Portuguese Foundation for Science and Technology (FCT) The online version is available at http://link.springer.com Filomena Gomes ( ) • Esteban San Martin Filomena Gomes. CERNAS...

Valuation of strawberry tree fruits (Arbutus unedo L.): from chemical characterization to the development of new products; Valorização do medronho (Arbutus unedo L.): da caracterização química ao desenvolvimento de novos produtos

Fonseca, Daniela Filipa da Silva
Fonte: Universidade de Aveiro Publicador: Universidade de Aveiro
Tipo: Dissertação de Mestrado
Português
Relevância na Pesquisa
101.61338%
The strawberry tree (A. unedo L.) is native from the Mediterranean region and it is implanted all over the Portuguese territory. In Portugal, A. unedo fruits have been used in the production of spirit, liqueurs and jam. Considering the wide distribution through Portuguese territory and the limited number of applications, it becomes crucial to value A. unedo fruits. For this purpose it is necessary to understand the chemical composition of A. unedo fruits, particularly during maturation and storage. Therefore, some quality parameters of A. unedo fruits were studied throughout maturation, particularly ºBrix, pH, titratable acidity, total phenolic content and antioxidant activity. In addition, the lipophilic extractives profile was assessed in ripen fruits. To promote the consumption of A. unedo fresh fruits it was studied the effect of cold storage (≈ 5 ºC) using fruits from two different ripeness degrees (19 and 21 ºBrix). The final phase of this thesis was focused on innovation and development of new food products based on A. unedo fruits. This study allowed us to conclude that throughout maturation, the ºBrix content and pH increased, whereas titratable acidity, total phenolic content and the antioxidant activity exhibited no remarkable tendency. Moreover...

Avaliação da qualidade ambiental da envolvente das Minas da Panasqueira. Vertente solo-água-Arbutus unedo. Um caso de estudo com orientação ambiental e social

Godinho, Berta Raquel Caetano
Fonte: Universidade Técnica de Lisboa Publicador: Universidade Técnica de Lisboa
Tipo: Dissertação de Mestrado
Publicado em //2009 Português
Relevância na Pesquisa
80.92917%
Mestrado em Engenharia do Ambiente - Tecnologias Ambientais - Instituto Superior de Agronomia; Panasqueira mine is one of the last three mines actually in labour. Tungsten, copper and tin are explored for over a century. The visual and chemical impact of the exploration is due to active and old huge tailings and due to draining from mine, water plant treatment and seepage water. The objective of this study was to evaluate the biogeochemical impact of the mine activity in soil, water and strawberry tree (Arbutus unedo L.). Soil is contaminated with copper, zinc, arsenic, tungsten and lead and the consequent impact was observed in microbial soil enzymes. Selective chemical extraction of trace elements allows to assess elements in soils and their absorption in plant roots and translocation to branches, leaves and fruits. Arbutus unedo is a manganese and zinc tolerant, and is not accumulator of trace elements. However, this species presented high concentration of cadmium in the aerial parts. The analysis of sediments and water from Ribeira of Bodelhão indicate that trace elements are dispersed from the primary contaminated area, to Zêzere River, where the dilution drastically eliminates the contamination effects. The strawberry tree fruits can be used to produce alcohol as they are not contaminated. A. unedo can be used in phytostabilisation programs of contaminated soils and tailings.

Identificação de marcadores SSR e de SNPs em medronheiro (Arbutus unedo L.) por sequenciação massiva paralela

Fazenda, Pedro Miguel Jesus
Fonte: ISA Publicador: ISA
Tipo: Dissertação de Mestrado
Publicado em //2013 Português
Relevância na Pesquisa
90.98266%
Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia; The strawberry tree (Arbutus unedo L.) is native to the Mediterranean region. The use of molecular markers in this species has been limited to the use of RAPDs, ISSRs as well to the cross-amplification of SSRs from other Ericaceae. In this work, we developed a protocol for extracting nuclear DNA from the strawberry tree and performed partial next-generation sequencing of the Arbutus unedo L. genome using the "Ion Torrent" (Life Technologies) platform. The next-generation sequencing resulted in 198,856 sequences ("raw data") with an average size of 123 bp, which were uploaded to the NCBI database "Sequence Read Archive" (SRA) with the accession number: SRX341237. Data analysis led to the identification of 1085 microsatellite-containing sequences, which were also uploaded with accession numbers: from KF023636 to KF024720 to the NCBI databases. Primers were designed for 18 microsatellite loci of which only three have proved to be polymorphic in a panel of 16 samples. Based on identified 25 SNPs one CAPS marker was developed, which despite being heterozygous revealed to be monomorphic among the 16 analyzed samples.

Contributo para o estudo do medronheiro (Arbutus unedo L.): caracterização morfológica de clones e fisiologia pós-colheita do fruto

Anastácio, Jorge Rosário
Fonte: ISA Publicador: ISA
Tipo: Dissertação de Mestrado
Publicado em //2014 Português
Relevância na Pesquisa
80.63182%
Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia; This study had two objectives that aim to contribute to a greater knowledge of Arbutus unedo. The first objective was to characterize morphologically different clones installed in a commercial orchard in Oleiros, Castelo Branco, in order to detect the morphological differences between them, as well as to monitor the vegetative cycle. The second objective was to study the physiological behavior of the fruit in the post-harvest period, in order to better assess the evolution of the maturation. The clones showed significant differences in the cross sectional area of the trunk and in the canopy height. Leaves showed significant differences in the length (L), width (W), L/W ratio, petiole length and leaf thickness. The percentage of trees with fruits, the average number of inflorescences and the number of fruits and shoots per inflorescence were significantly different. Fruits at different stages of maturity showed significant differences in firmness, L, hue and saturation. Except for the green fruits, all states of maturation showed an increase in the soluble solids content and pH while titratable acidity decreased. It was not found a marked evolution of vitamin C and total phenolic contents in the postharvest period. Considering the above...

Caracterização química e bioquímica dos frutos de ARBUTUS UNEDO L. e de RUBUS FRUTICOSUS AGG.: contribuição para a sua valorização

Cavaco, Teresa
Fonte: Universidade do Algarve Publicador: Universidade do Algarve
Tipo: Dissertação de Mestrado
Publicado em //2007 Português
Relevância na Pesquisa
101.333125%
Dissertação mest., Agricultura sustentável, Universidade do Algarve, 2007; O presente trabalho teve como principal objectivo caracterizar quimicamente dois frutos silvestres, o fruto de Arbutus unedo L (medronho) e o fruto de Rubus fruticosus Agg. (amora), que crescem espontaneamente na Serra do Caldeirão. Os parâmetros físico-químicos e bioquímicos estudados foram o pH, o ºBrix, a humidade, a cinza total, os iões cálcio, magnésio, sódio e potássio, o azoto total, a proteína total, os açúcares (frutose, glucose e sacarose) e as antocianinas (delfinidina 3- glucosido, delfinidina 3,5-diglucosido, cianidina 3-glucosido, cianidina 3,5-diglucosido, pelargonidina 3-glucosido e pelargonidina 3,5- diglucosido). Foram ainda determinados os parâmetros de cor no espaço CIE (L*, a* e b*). Observaram-se diferenças significativas nas várias determinações físico-químicas realizadas dependendo do ano e do local de colheita das amostras. Os frutos de Arbutus unedo L apresentaram na sua constituição teores das antocianinas delfinidina 3-glucosido e cianidina 3-glucosido de 0,44 mg e 0,13 mg por 100 g de fruto fresco, respectivamente. Os frutos de Rubus fruticosus Agg. apresentaram na sua composição concentrações das antocianinas cianidina 3-glucosido...

Validação do método de análise de espectrofotómetria de absorção atómica/emissão de chama para determinação de minerais na fermentação de medronho (Arbustus unedo L.)

Gomes, Carla Maria Longuinho
Fonte: Universidade do Algarve Publicador: Universidade do Algarve
Tipo: Dissertação de Mestrado
Publicado em //2007 Português
Relevância na Pesquisa
79.68136%
Dissertação mest., Qualidade em análises, Universidade do Algarve, 2006; O trabalho apresentado nesta dissertação teve como objectivo a validação de um método de análise por espectrofotómetria de absorção atómica/emissão de chama para determinação de minerais na fermentação de medronho (Arbutus unedo L.). O método estudado, foi desenvolvido e implementado no laboratório da FERN da Universidade do Algarve, sendo utilizado em rotina para quantificação da composição mineral de alguns produtos vegetais. No contexto da validação do método, apresentam-se os resultados da avaliação de alguns dos seus parâmetros característicos e da sua adequação à finalidade pretendida. A validação do método foi efectuada para 10 elementos minerais, tendo os elementos Ca, Cd, Cu, Fe, Mg, Mn, Pb e Zn sido validados por espectrofotómetria de absorção atómica e os elementos Na e K validados por emissão chama. Posteriormente procedeu-se a quantificação destes elementos em amostras de medronho fermentado, proveniente de uma fermentação anaeróbia de frutos de medronho. O processo de fermentação foi acompanhado pela recolha de 10 amostras aos tempos t= 0; 10; 12; 17; 18; 19; 24; 32; 48 e 65 dias. Verificou-se ser possível quantificar os elementos Ca...

Ecophysiology of after-fire resprouts of Arbutus unedo L

Savé, Robert; Alegre, Leonor; Pery, Maria; Terradas, Jaume
Fonte: Universidade Autônoma de Barcelona Publicador: Universidade Autônoma de Barcelona
Tipo: Artigo de Revista Científica Formato: application/pdf
Publicado em //1993 Português
Relevância na Pesquisa
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Plant-water relationships, leaf morphology and concentrations of pigments, proline, soluble sugars, anthocyans and nutrients of after wildfire resprout leaves of Arbutus unedo L. were com-pared with those of same age sprout leaves in unbumed plants. In spring samples, several significant differences appear: after-fire resprouts showed some juvenile characteristics, such as high tissue elasticity, small cells, low specific leaf weight, high Chla/Chlb ratio and a high anthocyan content; on the other hand, unbnrned plants showed drought tolerance characteristics by osmotic adjustment and lower tissue elasticity, larger cells, higher specific leaf weight and lower Chla/Chlb ratio. In fall samples, both kind of shoots showed drought tolerance by osmotic adjustment, low tissue elasticity, large cells and high specific leaf weight. Results show that after-fire resprouts have, at the beginning of their development, higher morphological and physiological plasticity that the new sprouts of unburned plants.; Es comparen les relacions hídriques, la morfologia foliar i les concentracions de pigments, prolina, sucres solubles, antocians i nutrients de les fulles de rebrots d'Arbutus unedo L. després d'un incendi, amb iguals característiques de les fulles de brots de plantes no cremades de la mateixa edat. A les mostres de primavera apareixen diverses diferències significatives: els rebrots...