Página 1 dos resultados de 1705 itens digitais encontrados em 0.008 segundos

Vitis vinifera leaves towards bioactivity

Fernandes, Fátima; Ramalhosa, Elsa; Pires, Patrícia; Andrade, João Verdial; Valentão, P.; Andrade, P.B.; Bento, Albino; Pereira, J.A.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
67.01%
The majority of the works about Vitis vinifera L. (grape) focus their attention on wine or its major by-product, the grape pomace. Nevertheless, leaves of V. vinifera also constitute an important matrix of this agro-industry. In this study, the chemical composition and antioxidant potential of V. vinifera leaves from twenty Portuguese varieties (white and red) were evaluated for the first time, concerning their reducing total capacity, reducing power and DPPH radical scavenging capacity. The phenolics presente in the aqueous extracts of V. vinifera leaves were trans-caffeoyltartaric and trans-coumaroyltartaric acids, myricetin-3-O-glucoside, quercetin-3-O-glucoside, quercetin-3-O-galactoside and kaempferol-3- O-glucoside, the last two compounds being the most predominant. Despite showing different activities profiles, all samples exhibited antiradical capacity against DPPH radical and reducing power in a concentration-dependent manner, with IC50 values below 800 g/mL. The obtained results are encouraging, increasing the possibility of taking profit from the great wastes produced by the grapes processing industry.

Efeito protetor de fitomedicamento Vitis Vinifera L na lesão renal aguda induzida pelo Tacrolimus; Protective effect of the phytomedicine Vitis Vinifera L in acute kidney injury by Tacrolimus

Silva, Wanessa Teixeira
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 04/11/2010 Português
Relevância na Pesquisa
67.4%
As lesões renais agudas (LRAs) nefrotóxicas correspondem a 30% dos casos de LRA. A nefrotoxicidade é efeito indesejável de diversos fármacos de uso rotineiro na clínica, entre eles as drogas imunossupressoras. A nefrotoxicidade do Tracolimus (Fk 506) é uma das causas de LRA após o transplante renal. Com a ampla utilização do Fk 506 nas terapias imunossupressoras e devido ao seu potencial nefrotóxico que pode levar à perda do enxerto, o objetivo desse estudo foi avaliar o efeito renoprotetor do extrato de Vitis vinifera L, um fitomedicamento com efeito antiinflamatório e antioxidante, na nefrotoxicidade induzida pelo Tacrolimus em ratos. Foram utilizados ratos Wistar, machos, adultos, pesando entre 250 - 300g, todos tratados 1x/dia por 5 dias, conforme os seguintes grupos: Salina (grupo controle) (NaCl 0,9%, 0,1ml por gavagem); Vitis (Vitis vinifera L 3mg/kg por gavagem), Fk (Tacrolimus 0,5mg/kg por gavagem) e Fk+Vitis (Tacrolimus 0,5mg/kg + 3mg/kg por gavagem). Foram avaliados a função renal (FR) (clearance de creatinina, método Jaffé); os peróxidos urinários (PU) (FOX-2), o malondealdeído (MDA-TBARS) e a histologia renal. Os dados desse estudo confirmaram a lesão nefrotóxica de caráter oxidativo induzida pelo Tacrolimus. A Vitis vinifera L demonstrou efeito renoprotetor significativo...

Alterações na produção de compostos fenólicos em culturas de células de Vitis vinifera eliciadas por Phaeomoniella chlamydospora; Changes in phenolic production of Vitis vinifera cell cultures induced by Phaeomoniella chlamydospora elicitation

Lima, M. R. M.; Guimarães, Orlando; Ferreres, Federico; Dias, Alberto Carlos Pires
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Publicado em //2004 Português
Relevância na Pesquisa
67.09%
A esca é uma doença que afecta plantas da espécie Vitis vinifera levando a importantes perdas na produção de vinho. O fungo Phaeomoniella chlamydospora (PC) é frequentemente associado a plantas de videira com esca e declínio das vinhas. Informação sobre a interacção deste fungo com a esca e declínio das vinhas ainda é escassa. Para estudar mecanismos de defesa da videira ao fungo PC utilizamos culturas in vitro de Vitis vinifera cv. Vinhão (Vv) eliciadas com biomasa autoclavada do fungo PC ou metiljasmonato (MeJ). Várias amostras foram tiradas durante o período experimental e a produção de compostos fenólicos por culturas de Vv foi analisada por HPLC-DAD e HPLC-MS/MS. A produção de compostos fenólicos das células de Vv alterou-se significativamente após exposição a PC e MeJ. As culturas de células de Vv eliciadas por PC e MeJ aumentam a produção total de stilbenos, quando comparadas com o controlo, nomeadamente em compostos do tipo viniferinas. Estes compostos são substâncias de conhecida acção antifúngica podendo ser importantes na defesa da planta contra infecção por PC. Assim, as culturas in vitro de Vv podem ser uma ferramenta importante, uma vez que oferecem um meio simples, rápido e selectivo para estudar as interacções planta/esca.; Fundação para a Ciência e a Tecnologia (FCT)

Differential phenolic production of Vitis vinifera cell cultures induced by Phaeomoniella chlamydospora elicitation

Lima, M. R. M.; Ferreres, Federico; Tavares, R. M.; Dias, Alberto Carlos Pires
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Publicado em //2004 Português
Relevância na Pesquisa
66.93%
Esca is a destructive disease, of complex aetiology, that affects grapevines in several countries around the world, specially in the Mediterranean, South Africa and California (where is known as “black measles”). In the last few years the disease became a major concern given to its dramatic incidence increase. Till now, does not exist a method to treat plants attacked by Esca. To study the putative response of V. vinifera plants to Paeomoniella chlamydospora (Pc), a fungus directly related with Esca, we utilized in cells cultures of Vitis vinifera cv. Vinhão (Vv) elicited with fungi Pc autoclaved extracts and methyl jasmonate (MeJ).; Fundação para a Ciência e a Tecnologia (FCT)

Modulation of phenolic production in Vitis vinifera cell cultures with Phaeomoniella chlamydospora and other elicitors

Lima, M. R. M.; Dias, Alberto Carlos Pires
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Publicado em /09/2005 Português
Relevância na Pesquisa
67.09%
Esca is a destructive disease that affects Vitis vinífera plants around the world leading to important losses in wine production. Phaeomoniella chlamydospora (Pc) is a fungus frequently associated with esca and grapevine decline. Information on the interaction of this fungus with Vitis vinifera is scarce. To study the defense mechanisms of Vitis plants to Pc we utilized cell suspension cultures of V. vinifera cv. Vinhão (Vv) elicited with fungus Pc, methyl-jasmonate (MeJ) and salicylic acid (SA). We also used cultures primed with MeJ or SA 24h before elicitation with Pc (MeJ+Pc or SA+Pc, respectively). Several culture samples were taken during the experimental period and phenolic production was evaluated by HPLC-DAD-MS; Fundação para a Ciência e a Tecnologia (FCT) - SFRH/BD/17944/2004

Differential phenolic production of Vitis vinifera cv. Alvarinho leaves affected with Esca disease

Lima, M. R. M.; Felgueiras, Mafalda; Cunha, Ana; Chicau, Gisela; Dias, Alberto Carlos Pires
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Publicado em /07/2006 Português
Relevância na Pesquisa
67.13%
Esca is a destructive disease that affects Vitis vinifera around the world leading to important losses in wine production. Information on the response of Vitis vinifera plants to this disease is still scarce. To study the defence mechanisms of Vitis plants to Esca, we analysed leaves from both infected and non-affected boughs of Vitis vinifera cv. Alvarinho, of the Vinho Verde region (North of Portugal). Phenolics were analysed by HPLC-DAD-MS and by multivariate statistical analysis.; Fundação para a Ciência e a Tecnologia (FCT) - SFRH/BD/17944/2004

Expression analysis of defence-related genes in Vitis vinifera cv. Vinhão cell cultures elicited with Phaeomoniella chlamydospora

Lima, M. R. M.; Dias, Alberto Carlos Pires
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Publicado em //2008 Português
Relevância na Pesquisa
66.93%
Esca is a destructive disease that affects Vitis vinífera plants around the world leading to important losses in wine production. Phaeomoniella chlamydospora (Pc) is a fungus frequently associated with esca and grapevine decline. To study the defence response, specifically gene activation, of grapevine to Pc we utilized in vitro cultures of V. vinifera cv. Vinhão (Vv) elicited with fungus extract. The expression of genes encoding pathogenesis‐related proteins (class 6 and class 10 PR proteins, β‐1,3‐glucanase, and class III chitinases) and genes involved in the octadecanoid (lipoxigenase) and phenylpropanoid (phenylalanine ammonia lyase and stilbene synthase) pathways were monitored by semi‐quantitative RT‐PCR.; Fundação para a Ciência e a Tecnologia (FCT) - Bolsa SFRH/BD/1/944/2004

Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : changes in phenolic production, oxidative state and expression of defence-related genes

Lima, M. R. M.; Ferreres, F.; Dias, Alberto Carlos Pires
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica
Publicado em /01/2012 Português
Relevância na Pesquisa
66.93%
Cell suspension cultures of Vitis vinifera cv. Vinhão (Vv) were used to study the putative response of V. vinifera to Phaeomoniella chlamydospora (Pc), a fungus frequently associated with esca and grapevine decline. Cells were elicited with a Pc autoclaved biomass extract and methyl jasmonate (MeJ). Phenolic production was evaluated by HPLC-DAD and HPLC-MS/MS. Phenolic production of Vv cells significantly changes after elicitation. Compared to control, Vv cells elicited by Pc extract increase their stilbene production 20-fold and those elicited by MeJ increase stilbenic production 9-fold. In both cases, there is de novo production of viniferin type compounds. We also analyzed the oxidative burst of Vv cells after elicitation with Pc extract and MeJ, using the probe 2′,7′-dichlorodihydrofluorescein diacetate. Adding Pc extract induces an oxidative burst thatshows a biphasic pattern in Vv cells. Moreover, the induction of 7 defence-related genes expression in Vvcell cultures upon Pc extract elicitation was investigated employing semi-quantitative RT-PCR. Elicitation increases the expression of class 6 and class 10 pathogenesis-related proteins, β-1,3-glucanase, class III chitinase, lipoxygenase, phenylalanine ammonia lyase and stilbene synthase. Therefore...

NMR metabolomics of esca disease-affected vitis vinifera cv. Alvarinho leaves

Lima, Marta; Felgueiras, Mafalda; Graça, Gonçalo; Rodrigues, João; Barros, António; Gil, Ana M.; Dias, Alberto Carlos Pires
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica
Publicado em /09/2010 Português
Relevância na Pesquisa
67%
Esca is a destructive disease that affects vineyards leading to important losses in wine production. Information about the response of Vitis vinifera plants to this disease is scarce, particularly concerning changes in plant metabolism. In order to study the metabolic changes in Vitis plants affected by esca, leaves from both infected and non-affected cordons of V. vinifera cv. Alvarinho (collected in the Vinho Verde region, Portugal) were analysed. The metabolite composition of leaves from infected cordons with visible symptoms [diseased leaves (dl)] and from asymptomatic cordons [healthy leaves (hl)] was evaluated by 1D and 2D 1H-nuclear magnetic resonance (NMR) spectroscopy. Principal component analysis (PCA) of the NMR spectra showed a clear separation between dl and hl leaves, indicating differential compound production due to the esca disease. NMR/PCA analysis allowed the identification of specific compounds characterizing each group, and the corresponding metabolic pathways are discussed. Altogether, the study revealed a significant increase of phenolic compounds in dl, compared with hl, accompanied by a decrease in carbohydrates, suggesting that dl are rerouting carbon and energy from primary to secondary metabolism. Other metabolic alterations detected comprised increased levels of methanol...

Phenolic profile of Sercial and Tinta Negra Vitis vinifera L. grape skins by HPLC-DAD-ESI-MSn: Novel phenolic compounds in Vitis vinifera L. grape

Perestrelo, Rosa; Lu, Ying; Santos, Sónia A. O.; Silvestre, Armando J. D.; Neto, Carlos P.; Câmara, José S.; Rocha, Sílvia M.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
67.11%
This study represents the first phytochemical research of phenolic components of Sercial and Tinta Negra Vitis vinifera L. The phenolic profiles of Sercial and Tinta Negra V. vinifera L. grape skins (white and red varieties, respectively) were established using high performance liquid chromatography-diode array detection-electrospray ionisation tandem mass spectrometry (HPLC-DAD-ESI-MSn), at different ripening stages (veraison and maturity). A total of 40 phenolic compounds were identified, which included 3 hydroxybenzoic acids, 8 hydroxycinnamic acids, 4 flavanols, 5 flavanones, 8 flavonols, 4 stilbenes, and 8 anthocyanins. For the white variety, in both ripening stages, hydroxycinnamic acids and flavonols were the main phenolic classes, representing about 80% of the phenolic composition. For red variety, at veraison, hydroxycinnamic acids and flavonols were also the predominant classes (71%), but at maturity, anthocyanins represented 84% of the phenolic composition. As far as we know, 10 compounds were reported for the first time in V. vinifera L. grapes, namely protocatechuic acid-glucoside, p-hydroxybenzoyl glucoside, caftaric acid vanilloyl pentoside, p-coumaric acid-erythroside, naringenin hexose derivate, eriodictyol-glucoside...

Efeitos da disponibilidade hídrica na fisiologia e produção de vitis vinifera l. Na região demarcada do douro

Sousa, Tiago Pinho Alves de
Fonte: Universidade de Trás-os-Montes e Alto Douro Publicador: Universidade de Trás-os-Montes e Alto Douro
Tipo: Tese de Doutorado
Português
Relevância na Pesquisa
67%
Tese de Doutoramento em Ciências Agronómicas; A temática das relações hídricas na vinha e sobretudo o seu impacto na produção não é consensual. Embora a rega seja uma prática agronómica comum em muitas zonas vitícolas do Globo, continuam a ser levantadas questões acerca das vantagens/inconvenientes que poderão advir da sua utilização. O presente trabalho pretende contribuir para a clarificação do tema e contextualizá-lo nas condições particulares da Região Demarcada do Douro (RDD). Em 2003 foi assim estabelecido um campo experimental na sub-região do Douro Superior numa parcela de Vitis vinífera L. cv. Tinta Roriz enxertada em 1103P, com o objectivo de avaliar o impacto de diferentes regimes hídricos no comportamento fisiológico da videira e as suas consequências no rendimento e composição dos mostos da RDD. Os tratamentos experimentais definidos diferiam quer na quantidade de água aplicada à vinha, quer no período da sua aplicação: não regado (NR), regado da floração ao pintor a 50% da ET (FP1), regado da floração ao pintor a 100% da ET (FP2), regado do pintor à maturação a 50% da ET (PM1), regado do pintor à maturação a 100% da ET (PM2) e regado da floração à maturação a 100% da ET (FM2). Foram utilizados vários métodos de avaliação do estado hídrico da videira...

Chloroplast genome diversity in Portuguese grapevine (Vitis vinifera L.) cultivars.

Castro, Isaura; Pinto-Carnide, Olinda; Ortiz, Jesús María; Martín, Juan Pedro
Fonte: Humana Press Inc Publicador: Humana Press Inc
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
66.97%
Grapevine chloroplast (cp) DNA diversity was analysed for the first time through amplification and digestion of fragments of the large single copy (LSC)region by polymerase chain reaction–restriction fragment length polymorphism methodology and also by amplification of three microsatellite loci, previously described as polymorphic in grapevine. Thirty-eight grapevine cultivars collected mainly in the North of Portugal, including some neglected cultivars, four international cultivars (Chasselas, Muscat of Alexandria, Muscat of Hamburg and Pinot) and Vitis riparia and Vitis rupestris, were used in this study with the main goal of finding out their pDNA diversity and compare the obtained results with previously published data on cultivars from other regions to ascertain their possible origin. Two different alleles were found in each of the three cpSSR loci. Allele variants of the three loci combined in a total of three different haplotypes (A, B and D). The most frequent haplotype, A, was previously reported as the most frequent in Iberian Peninsula and Occidental Europe. Haplotype B was unique to Rabigato, Muscat of Alexandria, V. riparia and V. rupestris. This haplotype was previously proposed to be an ancestral haplotype. Twenty-seven fragments of the LSC region of Vitis vinifera cpDNA were amplified and then digested with HinfI and TaqI restriction enzymes. Polymorphisms were found in the trnT-psbC (TC) and orf184-petA (OA) fragments. In the TC fragment...

Mineral stress affects the cell wall composition og grapevine (Vitis vinifera L.) callus

Fernandes, João C.; Garcia-Angulo, Penélope; Goulão, Luis F.; Acebes, José L.; Amâncio, Sara
Fonte: Universidade Técnica de Lisboa Publicador: Universidade Técnica de Lisboa
Tipo: Artigo de Revista Científica
Publicado em 28/01/2013 Português
Relevância na Pesquisa
66.92%
Versão provisória aceite p. publicação na Plant Science (ISSN 0168-9452)em 19-01-2013; Grapevine (Vitis vinifera L.) is one of the most economically important fruit crops in the world. Deficit in nitrogen, phosphorus and sulfur nutrition impairs essential metabolic pathways. The influence of mineral stress in the composition of the plant cell wall (CW) has received residual attention. Using grapevine callus as a model system, 6-weeks deficiency of those elements caused a significant decrease in growth. Callus CWs were analyzed by Fourier transform infrared spectroscopy (FT-IR), by quantification of CW components and by immunolocalization of CW epitopes with monoclonal antibodies. PCA analysis of FT-IR data suggested changes in the main components of the CW in response to individual mineral stress. Decreased cellulose, modifications in pectin methyl esterification and increase of structural proteins were among the events disclosed by FT-IR analysis. Chemical analyses supported some of the assumptions and further disclosed an increase in lignin content under nitrogen deficiency, suggesting a compensation of cellulose by lignin. Moreover, polysaccharides of callus under mineral deficiency showed to be more tightly bonded to the CW...

Functional genomic analysis of heat stress in Vitis vinifera

Diz, Raimundo Maximino Martins
Fonte: Universidade Nova de Lisboa Publicador: Universidade Nova de Lisboa
Tipo: Dissertação de Mestrado
Publicado em /01/2015 Português
Relevância na Pesquisa
67%
Grapevine (Vitis vinifera) is one of most agro-economically important fruit crops worldwide, with a special relevance in Portugal where over 300 varieties are used for wine production. Due to global warming, temperature stress is currently a serious issue affecting crop production especially in temperate climates. Mobile genetic elements such as retrotransposons have been shown to be involved in environmental stress induced genetic and epigenetic modifications. In this study, sequences related to Grapevine Retrotransposon 1 (Gret1) were utilized to determine heat induced genomic and transcriptomic modifications in Touriga Nacional, a traditional Portuguese grapevine variety. For this purpose, growing canes were treated to 42 oC for four hours and leaf genomic DNA and RNA was utilized for various techniques to observe possible genomic alterations and variation in transcription levels of coding and non-coding sequences between non-treated plants and treated plants immediately after heat stress (HS-0 h) or after a 24 hour recovery period (HS-24 h). Heat stress was found to induce a significant decrease in Gret1 related sequences in HS-24 h leaves, indicating an effect of heat stress on genomic structure. In order to identify putative heat induced DNA modifications...

Multiple glucosyltransferase activities in the grapevine Vitis vinifera L

Ford, C.; Hoj, P.
Fonte: Wiley Interscience Publicador: Wiley Interscience
Tipo: Artigo de Revista Científica
Publicado em //1998 Português
Relevância na Pesquisa
67.04%
The conjugation by glycosyltransferases of sugars to primary and secondary metabolites is widespread among plants and almost certainly a prerequisite for the accumulation of secondary metabolites at high levels. In the case of the grapevine, Vitis vinifera, modulating the levels of specific secondary berry metabolites is a desirable outcome for the development of wines with particular style characteristics. This can be achieved only by a thorough understanding of the processes underlying glucoside formation and accumulation during berry development. Using protein extracts prepared from leaves and berries of Vitis vinifera cvs, we show here that glucosyltransferase activities can be detected against a wide range of substrates. Among the substrates glucosylated were several classes of phenylpropanoids, including flavonols, anthocyanidins, flavanones, flavones, isoflavones, and a stilbene. Additionally, simple phenols and monoterpenes were glucosylated. Total soluble leaf proteins subjected to ion-exchange chromatography separated into fractions with differing glucosyltransferase activities. This provided strong evidence for the existence both of multiple distinct enzyme activities, and multiple isozymes catalysing identical reactions. Polyclonal antiserum raised to a V. vinifera UDP-glucose:anthocyanidin glucosyltransferase was used to demonstrate the existence of multiple glucosyltransferases in berries and leaves of the grapevine cvs Muscat of Alexandria and Shiraz...

Composition and synthesis of raphide crystals and druse crystals in berries of Vitis vinifera L. cv. Cabernet Sauvignon: Ascorbic acid as precursor for both oxalic and tartaric acids as revealed by radiolabelling studies

DeBolt, S.; Hardie, J.; Tyerman, S.; Ford, C.
Fonte: Australian Society of Viticulture and Oenology Publicador: Australian Society of Viticulture and Oenology
Tipo: Artigo de Revista Científica
Publicado em //2004 Português
Relevância na Pesquisa
66.93%
Biomineralisation in the fleshy pericarp of berries of Vitis vinifera L. gives rise to crystals of two distinct forms, viz. raphides and druses, which are found in exocarp and endocarp cells respectively. Druses have generally been considered as crystalline aggregates of calcium oxalate. However, the organic moiety of raphide crystals has been commonly accepted as tartrate, although we have found no analytical data to support that assumption. We now present TEM and X-ray powder diffraction analysis data showing that raphide crystals of V. vinifera berries are composed of calcium oxalate monohydrate. This work also established ascorbic acid as the biosynthetic precursor of both oxalic and tartaric acids. When ascorbic acid labelled with ¹⁴C at position 1 was introduced into berries via the rachis, 21% and 52% of the added radiolabel was recovered as oxalic and tartaric acids respectively. Purified crystals from the radiolabelled grape berries contained approximately 20% of the original radioactivity, further confirming the role of ascorbic acid in oxalic acid biosynthesis. To our knowledge, this is the first evidence to be published on the formation of oxalic and tartaric acids from ascorbic acid via two distinct pathways operating within the same physiological entity (organ).; Seth Debolt...

Anthocyanic vacuolar inclusions (AVIs) selectively bind acylated anthocyanins in Vitis vinifera L. (grapevine) suspension culture

Conn, S.; Zhang, W.; Franco, C.
Fonte: Kluwer Academic Publ Publicador: Kluwer Academic Publ
Tipo: Artigo de Revista Científica
Publicado em //2003 Português
Relevância na Pesquisa
66.93%
Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolated from the same callus accumulating anthocyanin in the dark, yet varying in their anthocyanin profiles and accumulation. An intermediate-pigmented line (FU-1) with a 1.3:1 ratio of acylated:non-acylated anthocyanins, a colour value of 0.84 units and cyanidin and peonidin as the dominant species was compared with a high-pigmented line (FU-2) with a 1.2:1 ratio of acylated:non-acylated anthocyanins, a colour value of 3.72 units and malvidin predominating. The profile of AVI-bound anthocyanins showed an increase in acylated anthocyanins in both lines of approx. 28–29%, with no apparent preference for anthocyanin species. This resulted in a ratio of acylated:non-acylated anthocyanins of 6.2:1 for FU-1 and 4.9:1 for FU-2. The reasons for the selectivity of the AVIs for acylated (specifically p-coumaroylated) species compared with the whole cell profile are discussed.; The original publication can be found at www.springerlink.com

Phenolic profile of Sercial and Tinta Negra Vitis vinifera L. grape skins by HPLC–DAD–ESI-MSn: novel phenolic compounds in Vitis vinifera L. grape

Perestrelo, Rosa; Lu, Ying; Santos, Sónia A. O.; Silvestre, Armando J. D.; Neto, Carlos P.; Câmara, José S.; Rocha, Sílvia M.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em /11/2012 Português
Relevância na Pesquisa
67.11%
This study represents the first phytochemical research of phenolic components of Sercial and Tinta Negra Vitis vinifera L. The phenolic profiles of Sercial and Tinta Negra V. vinifera L. grape skins (white and red varieties, respectively) were established using high performance liquid chromatography–diode array detection–electrospray ionisation tandem mass spectrometry (HPLC–DAD–ESI-MSn), at different ripening stages (véraison and maturity). A total of 40 phenolic compounds were identified, which included 3 hydroxybenzoic acids, 8 hydroxycinnamic acids, 4 flavanols, 5 flavanones, 8 flavonols, 4 stilbenes, and 8 anthocyanins. For the white variety, in both ripening stages, hydroxycinnamic acids and flavonols were the main phenolic classes, representing about 80% of the phenolic composition. For red variety, at véraison, hydroxycinnamic acids and flavonols were also the predominant classes (71%), but at maturity, anthocyanins represented 84% of the phenolic composition. As far as we know, 10 compounds were reported for the first time in V. vinifera L. grapes, namely protocatechuic acid-glucoside, p-hydroxybenzoyl glucoside, caftaric acid vanilloyl pentoside, p-coumaric acid-erythroside, naringenin hexose derivate, eriodictyol-glucoside...

Carpometria da grainha na identificação de ancestraissel vagens de Vitis vinifera L.

Cunha,J.; Cunha,J. P.; Carneiro,L. C.; Fevereiro,Pedro; Eiras-Dias,J.E.
Fonte: INIAV - DOIS PORTOS (Ex-Estação Vitivinícola Nacional) Publicador: INIAV - DOIS PORTOS (Ex-Estação Vitivinícola Nacional)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2007 Português
Relevância na Pesquisa
67.19%
No presente trabalho, são estudadas as medidas da grainha como método para descriminar variedades cultivadas de Vitis vinifera ssp vinifera e plantas de Vitis vinifera ssp sylvestris.Caracterizaram-se ao nível da grainha 10 plantas femininas produtoras de bagos das populações selvagens, 61 variedades de videiratradicionalmente utilizadas em Portugal e 10 variedades internacionalmente conhecidas.O presente trabalho permite concluir que a razão largura/comprimento é o parâmetro que melhor descrimina as plantas em estudo. O métododesenvolvido pode vir a ser útil em estudos de achados de jazidas arqueológicas dado ser um método não destrutivo.Observou-se que as variedades Ferral (50104), Olho de Lebre (51109) e Uva Rei (50713) apresentam um quociente entre 0,65=QC=0,75, oque adverte para a hipótese de parentesco entre estas e as sylvestris.Estes aspectos justificam o parâmetro largura da grainha, um dado passaporte, para aplicação do quociente, no código de caracteresdescritivos de variedades e espécies de Vitis do OIV.

Comparação ampelométrica de populações selvagens de Vitis Vinífera L. e de castas antigas do Sul de Portugal

Coelho,I.; Cunha,J.; Cunha,J.P.; Carneiro,L. C.; Castro,R.; Dias,J. E. Eiras
Fonte: INIAV - DOIS PORTOS (Ex-Estação Vitivinícola Nacional) Publicador: INIAV - DOIS PORTOS (Ex-Estação Vitivinícola Nacional)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2004 Português
Relevância na Pesquisa
67%
Neste estudo pretende-se analisar as semelhanças existentes entre 11 castas antigas cultivadas no Alentejo e 3 populações de Vitis vinifera spp sylvestris (Gmel) Hegi recentemente detectadas na mesma região, usando métodos ampelometricos. Assim, caracterizaram-se as castas e as populações selvagens através de medições lineares na folha adulta. Para a comparação entre as duas subespécies de Vitis vinifera L. foram utilizados métodos de taxonomia numérica. A comparação entre as castas cultivadas e as videiras selvagens mostrou que é possível distinguilas através dos parametros utilizados na caracterização ampelométrica. Esta distinção decorre principalmente no recorte da folha e no tamanho do lóbulo C, consequentemente no grau de abertura do seio peciolar. Verificou-se também que a casta Marufo apresenta maior semelhança com as plantas da população silvestre de Castelo Branco do que com as castas cultivadas. Pode ainda concluir-se que, as videiras sylvestris possuem seio peciolar mais aberto e folhas mais pequenas que as variedades cultivadas.