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Histochemistry and histology in planktonic ecophysiological processes determination in a temperate estuary (Mondego River estuary, Portugal)

Pastorinho, M. R.; Antunes, C. P.; Marques, J. C.; Pereira, M. L.; Azeiteiro, U. M. M.; Morgado, F. M.
Fonte: Universidade de Coimbra Publicador: Universidade de Coimbra
Tipo: Artigo de Revista Científica Formato: aplication/PDF
Português
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Zooplankton communities in temperate climates exhibit well documented seasonal variability. The reasons proposed to explain this kind of behaviour range from the effect of physical-chemical conditions (salinity, nutrients, temperature) growth rates, mortality, migration rates, food availability and predation. It is noticeable that the majority of the factors mentioned are external to the organisms, being environmental factors responsible for time dependent changes in zooplankton abundance. A question may arise from this conclusion, namely, "Could zooplankton blooms, in temperate climates, be mainly modelled by the internal physiological predispositions of the individual organisms?" Or, in a more "functional" formulation, "Could gonads be the baseline regulators of zooplankton abundance?". This study answers this pertinent question, assuming as a basic assumption that the reproductive potential of these organisms is constant, being controlled by extrinsic factors. For this purpose, histological and histochemical techniques were applied to the mysid Mesopodopsis slabberi, a key species in the Mondego River estuary, in order to establish a scale of gonadic development that would allow comparisons between high and low density periods. Histology reveals itself as the ideal base to establish a tool for the evaluation of the gonadic masses by allowing to calculate oocyte dimensions (trough biometry) and determine its maturity status through calculation of the N/C ratio and histochemical analysis (PAS and Tetrazonium reaction for proteins). Upon creation...

Integração do tendão do músculo semitendíneo na reconstrução do ligamento cruzado anterior: estudo biomecânico, histológico e ressonância magnética em coelhos; The incorporation of the semitendinous tendon autograft at the femoral femoral bone tunnel after anterior cruciate ligament reconstrcuction in rabbits: biomechanical histology and magnetic resonance image analysis

Pereira, Paulo Paes
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 05/12/2006 Português
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O estudo analisa experimentalmente a integração tendinosa no túnel ósseo femoral na reconstrução do ligamento cruzado anterior do joelho esquerdo com o tendão do músculo semitendíneo autólogo, utilizando imagens de ressonância magnética, teste biomecânico e análise histológica em 15 coelhos da raça Nova Zelândia. Após os períodos de quatro, oito e doze semanas do procedimento cirúrgico, os animais foram submetidos ao exame de ressonância magnética para avaliar o túnel femoral dos joelhos. A seguir os animais foram eutanasiados e os joelhos foram submetidos a testes de tração em uma máquina de ensaios mecânicos Kratos para verificar a integração do enxerto nos túneis e a exame histológico do túnel femoral. A análise dos resultados demonstrou integração mecânica do tendão no túnel femoral a partir da 4ª semana em todos os animais estudados e observou-se na histologia e nas imagens da ressonância magnética alterações do enxerto e da área ao redor de forma heterogênea, sugerindo um processo de cicatrização do tendão-osso, porém não se pode afirmar que ocorria a integração até a 12 semanas.; The purpose was to verify the incorporation (healing) of the graft of the semitendinous tendon into the femoral bone tunnel after an anterior cruciate ligament reconstruction...

Comparação das dimensões das artérias coronárias e da composição da placa aterosclerótica entre a angiografia coronária por tomografia de 64 colunas de detectores e a ultrassonografia intracoronária com a técnica de histologia virtual; Comparison of coronary artery dimensions and atherosclerotic plaque composition between coronary angiography by 64-slice computed tomography and by virtual histology intravascular ultrasound technique

Falcão, João Luiz de Alencar Araripe
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 24/02/2010 Português
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Introdução: No momento, pouco se conhece sobre o desempenho diagnóstico da angiotomografia de coronárias com 64 colunas de detectores (Angio-TC 64) frente aos achados do ultrassom intracoronário com técnica de histologia virtual (USIC-HV). Este estudo compara a capacidade diagnóstica de ambos os métodos para a avaliação das dimensões vasculares e para avaliação da composição da placa aterosclerótica através da análise de toda extensão dos três vasos epicárdicos principais. Métodos e Resultados: Um total de 21 pacientes com diagnóstico de doença arterial coronária obstrutiva foi incluído neste estudo. Angio-TC 64 foi realizada em todos os pacientes antes da realização do USIC-HV, com intervalo<72horas entre os dois exames. No total, 70 vasos foram avaliados (3,3 vasos por paciente) e divididos em 641 subsegmentos de 4 mm de extensão cada. Um total de 5.972 cortes seccionais de USIC-HV e 5.233 cortes seccionais da Angio-TC 64 foram analisados. As medidas de área luminar, área do vaso, área da parede arterial e carga de placa à Angio-TC 64 e ao USIC-HV foram significativamente correlacionadas (r-Spearman: 0,81; 0,78; 0,55 e 0,49; respectivamente - p<0,001 para todas as correlações). A Angio-TC 64 subestimou a área luminar ao USIC-HV (em mediana: 0...

Utilização do ultra-som intracoronário com Histologia Virtual® na identificação de lesões propensas à reestenose após o implante de stents; Application of Virtual Histology®-intravascular ultrasound (VH-IVUS) to identify restenosis-prone lesions after baremetal and sirolimus-eluting stents

Siqueira, Dimytri Alexandre de Alvim
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 13/07/2011 Português
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A intervenção percutânea com implante de stents constitui-se na principal forma de revascularização miocárdica empregada, e associa-se à redução de sintomas, à melhoria na qualidade de vida e ao aumento da sobrevida em pacientes acometidos por síndrome coronária aguda. Entretanto, o implante de stents pode acompanhar-se de exagerada hiperplasia intimal, principal determinante da reestenose destes dispositivos. Diversos fatores clínicos, anatômicos e técnicos associam-se a maiores taxas de reestenose, porém a hipótese de que o tipo ou a composição da lesão aterosclerótica tratada possa correlacionar-se com o fenômeno ainda não foi esclarecida. Determinar a relação entre a composição da placa aterosclerótica tratada conforme análise pela Histologia Virtual® - e a magnitude da hiperplasia intimal após o implante de stents farmacológicos e não-farmacológicos. No período de setembro de 2008 a novembro de 2009, selecionamos 52 pacientes prospectivos com o diagnóstico de síndrome coronária aguda com ou sem supra ST, submetidos à cinecoronariografia e candidatos à intervenção percutânea. Estes foram randomizados para o tratamento com stents farmacológicos com sirolimus (Cypher® Cordis, Johnson & Johnson) ou com stents não-farmacológicos (Driver®...

Investigação das modificações na geometria vascular nas bordas de stents farmacológicos e não-farmacológicos e a correlação com a composição dos ateromas: estudo seriado com ultrassom intracoronário e Histologia Virtual®; Investigation of the modifications in vascular geometry at the edges of bare-metal and drug-eluting stents and the correlation of modifications in plaque composition: a serial with grey-scale intravascular ultrasound and Virtual Histology(TM)

Costa Junior, José de Ribamar
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 07/07/2011 Português
Relevância na Pesquisa
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Até o momento, pouco se sabe sobre a influência da modificação na composição do ateroma nas bordas dos stents e a ocorrência de alterações na geometria vascular. Este estudo objetiva correlacionar, utilizando de maneira seriada (pós-implante do stent e reestudo aos nove meses) o ultrassom monocromático e a Histologia Virtual®, as modificações na composição dos ateromas nas bordas proximais e distais de stents nãofarmacológicos e farmacológicos e as alterações ocorridas nas dimensões do vaso, luz e placa que possam explicar a ocorrência da reestenose nestes segmentos. Estudo prospectivo, de centro único, que randomizou (1:1) pacientes com síndrome coronária aguda para receberem stents nãofarmacológicos (Driver®, n=20 pacientes) ou farmacológicos (Cypher®, n=20 pacientes). Após a realização do procedimento, todos os pacientes submeteram-se a avaliação com ultrassom e Histologia Virtual®, que foi repetido ao final de nove meses de seguimento. O objetivo primário foi avaliar as modificações na área do vaso, luz e placa ao ultrassom e na composição do ateroma pela Histologia Virtual® no período entre o implante e o reestudo, buscando correlacionar as alterações no ateroma com as modificações na geometria vascular. Observou-se que na borda proximal...

Histologia e bioquímica da interação entre porta-enxertos de citros resistente e suscetíveis a Phytophthora nicotianae; Histology and biochemistry of the interaction between resistant and susceptible citrus rootstocks to Phytophthora nicotianae

Escanferla, Maria Eugenia
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 14/12/2011 Português
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No Brasil Phytophthora nicotianae é um dos principais agentes causadores da podridão do pé e de raízes e radicelas em citros. As doenças provocadas pelo gênero ocasionam danos elevados à produção agrícola e o uso de porta-enxertos resistentes é medida importante para controle. Nesse trabalho objetivou-se estudar aspectos relacionados à histologia e bioquímica da interação entre P. nicotianae e porta-enxerto de tangerineira Sunki (suscetível) e citrumeleiro Swingle (resistente). Para tal, raízes de plântulas desses genótipos com dois, três ou seis meses foram inoculadas com suspensão de 105 zoósporos/mL de P. nicotianae e mantidas a 25°C. As análises foram realizadas em microscópio de luz (ML) e confocal (MC), em cromatografia líquida de alta eficiência (CLAE), em cromatógrafo a gás (CG) e em microscópio eletrônico de varredura (MEV). Em ML, as secções de raízes coradas com azul de toluidina um, dois, quatro e seis dias após a inoculação (dai) indicaram diferenças entre os porta-enxertos no modo e na velocidade de colonização do patógeno. O hospedeiro resistente apresentou menor número de hifas nos tecidos e essas se localizaram principalmente nos espaços intercelulares. Em MC, analisou-se a distribuição da elicitina do patógeno em secções de raízes um...

Recovery of spermatogenesis after microsurgical subinguinal varicocele repair in azoospermic men based on testicular histology

Esteves,Sandro C.; Glina,Sidney
Fonte: Sociedade Brasileira de Urologia Publicador: Sociedade Brasileira de Urologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2005 Português
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36.72%
OBJECTIVE: Analyze whether testicular histologic patterns from a group of azoospermic men with varicocele is predictive of treatment outcome after subinguinal microsurgical varicocele repair. MATERIALS AND METHODS: Seventeen azoospermic men underwent bilateral open single testis biopsy and microsurgical subinguinal repair of clinical varicoceles. RESULTS: Histopathology of testicular biopsies revealed hypospermatogenesis (HYPO) in 6 men, maturation arrest (MA) in 5, and Sertoli cell-only (SCO) in 6. Overall, presence of spermatozoa in the ejaculates was achieved in 47% (8/17) of men after varicocele repair, but only 35% (6/17) of them had motile sperm in their ejaculates. Only men with testicular histology revealing HYPO (5/6) or maturation arrest (3/5) had improvement after surgery. Median (25% - 75% percentile) postoperative motile sperm count for both groups were 0.9 X 106/mL (0.1-1.8 X 106/mL) and 0.7 X 106/mL (0.1-1.1), respectively (p = 0.87). The mean time for appearance of spermatozoa in the ejaculates was 5 months (3 to 9 months). One (HYPO) of 8 (12.5%) men who improved after surgery contributed to an unassisted pregnancy. Postoperative testicular biopsies obtained from patients who had no improvement after surgery revealed that testicular histology diagnosis remained unchanged. Successful testicular sperm retrieval for intracytoplasmic sperm injection (ICSI) was achieved in 4 of 9 (44.4%) individuals who did not improve after surgery...

Gathering the evidence: Cord gases and placental histology for births with low Apgar scores

Wong, L.; MacLennan, A.
Fonte: Blackwell Publishing Asia Publicador: Blackwell Publishing Asia
Tipo: Artigo de Revista Científica
Publicado em //2011 Português
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Background: Acute intrapartum hypoxia is an uncommon cause of cerebral palsy. The exclusion of acute intrapartum hypoxia utilizes two vital pieces of information that can be obtained at the time of birth: (i) cord blood gas to exclude a severe metabolic acidosis, and (ii) placental histology to suggest an alternative aetiology other than acute intrapartum hypoxia. Although recommendations exist to encourage this practice in high-risk deliveries, their compliance in an Australian setting is not known. Aims: To evaluate the frequency and utility of cord blood gases and placental histology following delivery with an Apgar score ≤6 at five minutes. Methods: A retrospective study of 12,887 consecutive deliveries at a tertiary obstetric centre, of which 100 live births had Apgar scores ≤6 at five minutes. Cord blood gases and placental histology were examined. There were also 132 stillbirths where placental histology was sought. Results: Cord gases were measured in 52 of 100 live births with a low Apgar score. Seven of these had severe metabolic acidaemia and 26 had normal cord gases. Placental histology was requested in 40 of these births and 30 showed abnormal histology, suggesting alternative aetiologies. Of the 132 stillbirths, placental histology was available in 50. Abnormal histology was present in 39 of these stillbirths. Conclusions: Cord gases and placental histology should be sought in all babies with low Apgar scores for the benefit of understanding causation...

Bewertung der Photodynamischen Diagnostik mit 5-Aminolävulinsäure beim Basalzellkarzinom durch 3-D-Histologie; Evaluation of the photodynamic diagnosis on basal cell carcinoma with 3-d-histology

Steinat, Daniela
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
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HINTERGRUND: Die topische Applikation von 5-Aminolävulinsäure (5-ALA) soll der Detektion epithelialer Hauttumoren im Rahmen der Photodynamischen Diagnostik (PDD) dienen. Über die Häm-Biosynthese wird 5-ALA, welches nach der Applikation in neoplastischen Geweben akkumulieren soll, in den endogenen Photosensibilisator Protoporphyrin IX (PpIX) umgewandelt. PpIX erzeugt unter Wood-Licht-Exposition (350-400nm) eine intensive Rotfluoreszenz. Diese tumorspezifische Fluoreszenz soll eine vielversprechende diagnostische Methode zur Detektion maligner Hautveränderungen und ihrer Grenzen darstellen. FRAGESTELLUNG: Das Ziel dieser Arbeit war es, festzustellen, ob die PDD mit 5-ALA zu einer Verbesserung bei der Diagnostik von Basalzellkarzinomen, insbesondere der Tumorgrenzen und damit zu einer Optimierung der chirurgischen Therapie führt. MATERIAL UND METHODEN: Es wurden bei 61 Patienten insgesamt 91 Hautveränderungen, welche den klinischen Verdacht auf ein Basalzellkarzinom aufwiesen, untersucht. Eine 20%ige 5-ALA-Creme wurde topisch auf die Läsionen mit einem 1cm breiten Rand aufgetragen. Nach vierstündiger Okklusion erfolgte eine Markierung der klinischen Tumorgrenzen, dann eine Markierung eventuell zusätzlich unter Wood-Licht fluoreszierender Areale. Das so bestimmte Gebiet wurde mit einem adäquaten Sicherheitsabstand exzidiert und mit Hilfe der lückenlosen Schnittrandhistologie (3-D-Histologie) untersucht. ERGEBNISSE: Eine exakte Demarkierung vom gesunden umgebenden Gewebe erfolgte in nur 41% aller Fälle...

3D-histology and malignant soft tissue sarcomas of the skin : Local recurrence rate and growth pattern; 3D-Histologie und maligne Weichteilsarkome der Haut : Lokalrezidive und Wachstumsmuster

Eder, Simone
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
36.84%
BACKGROUND: Malignant soft tissue sarcomas of the skin are rare tumour entities with a high tendency to local recurrence and metastasis. Surgery is still the principal therapeutic means in treatment of soft tissue sarcomas. Conventional surgery is based on horizontal histological evaluations, however leaves diagnostic gaps. The introduction of Mohs micrographic lead to more specific detection of subclinical spreads and following re-excisions through histological examination of serial horizontal wound margins. Breuninger et al.(1988) later introduced the technique of complete three-dimensional histology including the basis with paraffin sections which is now called 3D-histology. OBJECTIVE: Purpose of this retrospective study was to evaluate the long-term follow-up, the clinicopathological characteristics and the microscopically growth pattern of malignant soft tissue sarcomas of the skin in patients treated by surgery with 3D-histology at the Department of Dermatology at the University of Tübingen. MATERIAL AND METHODS: Medical records of 131 patients with diagnosis of malignant soft tissue sarcoma of the skin that have been treated by surgery and 3D-histology at our department since 1982 have been reviewed and included. The diagnosis of malignant soft tissue sarcoma of the skin included histology of dermatofibrosarcoma protuberans(DFSP)...

Pattern of local, regional and distant recurrence of Merkel cell carcinoma after excision with 3D-histology; Das Muster von Lokal- und Regionalrezidiven sowie Fernmetastasen des Merkelzellkarzinoms nach Exzision mittels 3D-Histologie

Taha, Sarah
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
36.72%
Background: Merkel Cell Carcinoma (MCC) is a rare but highly malignant neoplasm of the skin that was first reported by Toker in 1972. Wide local excision has been recommended whenever possible, but especially in the head and neck region where wide margins are not amenable, the complete histology of three-dimensional excisional margins (3D-Histology) is advocated. Methods: The course of Merkel Cell Carcinoma Disease in 33 Patients that have beem treated at the University Hospital of Dermatology in Tuebingen was investigated. 3D-Histology which is performed with a rush-paraffin technique and has been invented by Professor Dr. Breuninger in 1988 was the main tool of therapy. Results: The age limilts of the patient’s collective were located between 51 and 87( median = 70). The majority of the primary tumor localizations were diagnosed in the head and neck region with 42.4%. The area of the upper limbs represented 30.3% of all primary sites and the region of the lower limbs had a fraction of 27.3%. No single patient had a primary affection of the trunk. These figures underline the assumed influence of sun exposure in the emergence of MCC. 86.7% of the patients that have been operated at the University Hospital of Dermatology in Tuebingen could be released with RO-Resection after the first operation...

Motivational component profiles in university students learning histology: a comparative study between genders and different health science curricula

Campos-S??nchez, Antonio; L??pez N????ez, Juan Antonio; Carriel Araya, V??ctor Sebasti??n; Mart??n-Piedra, Miguel ??ngel; Sola Mart??nez, Tom??s; Alaminos Mingorance, Miguel
Fonte: Biomed Central Publicador: Biomed Central
Tipo: Artigo de Revista Científica
Português
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Background: The students' motivation to learn basic sciences in health science curricula is poorly understood. The purpose of this study was to investigate the influence of different components of motivation (intrinsic motivation, self-determination, self-efficacy and extrinsic -career and grade-motivation) on learning human histology in health science curricula and their relationship with the final performance of the students in histology. Methods: Glynn Science Motivation Questionnaire II was used to compare students' motivation components to learn histology in 367 first-year male and female undergraduate students enrolled in medical, dentistry and pharmacy degree programs. Results: For intrinsic motivation, career motivation and self-efficacy, the highest values corresponded to medical students, whereas dentistry students showed the highest values for self-determination and grade motivation. Genders differences were found for career motivation in medicine, self-efficacy in dentistry, and intrinsic motivation, self-determination and grade motivation in pharmacy. Career motivation and self-efficacy components correlated with final performance in histology of the students corresponding to the three curricula. Conclusions: Our results show that the overall motivational profile for learning histology differs among medical...

Learning and teaching histology: traditional and computational methods

Capela e Silva, Fernando; Rato, Luis Miguel; Lopes, Orlando Silva
Fonte: Universidade de Évora Publicador: Universidade de Évora
Tipo: Aula Formato: 56857 bytes; application/pdf
Português
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The traditional methodology used in histology laboratory instruction of histology is based primarily on the use of photonic microscopes and histological slides, which did not allow the simultaneous observation by several people, prevents an interactive discussion, making the laboratory teaching-learning process less motivating and advantageous. The use of various computer-based technologies (including virtual microscopy and virtual slides), as well as video projection, using digital cameras attached to the microscope in histology laboratory instruction is an interesting alternative method to offset the disadvantages inherent to the conventional methodology. Advances in computer sciences allow us to play a considerable amount of images and transfer them to monitor students' computers, resulting in its comfortable and objective viewing, thus facilitating the recognition of tissues and structures and their histomorphometric study through computer programs. In addition), the use of platforms for distance learning (e.g. Moodle), also allows the study of histology to be undertaken outside the classroom. The objective of this paper is to evaluate the advantages and disadvantages of traditional methods of histological sections visualization in photonic microscope...

A comparative study of the ovarian histology of eyestalk ablated and unablated Farfantepenaeus paulensis after spawning

Peixoto, Silvio Ricardo Maurano; Cavalli, Ronaldo Olivera; D'Incao, Fernando; Wasielesky Junior, Wilson Francisco Britto; Milach, Ângela Machado
Fonte: Universidade Federal do Rio Grande Publicador: Universidade Federal do Rio Grande
Tipo: Artigo de Revista Científica
Português
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36.58%
The effect of unilateral eyestalk ablation on ovarian histology of Farfantepenaeus paulensis after spawning was evaluated in the present study. Wild females were captured in deep-sea waters of southern Brazil (27 o S) and randomly divided in two groups: unilaterally eyestalk ablated and unablated (intact) females. A total of six eyestalk ablated and five unablated females were sampled after spawning in separated tanks. Morphological variables were recorded and ovaries were histologically evaluated according to oocyte type and diameter. In the ovarian tissue of unablated females, basophilic oocytes (48.8 ± 18.7 µm) were dominant (99.7 ± 0.6%), with presence significantly lower in the ablated females (93.5 ± 13.8%). Larger acidophilic oocytes (114.9 ± 16.9 µm), with yolk granules in the cytoplasm, were detected only in the ovaries of ablated females. The significantly higher occurrence of atretic oocytes (4.8 ± 10.3%) is another distinguishable feature in the ovaries of ablated females compared with the unablated ones. The presence of advanced yolky oocytes in ablated females just a few hours after spawning, may indicate the effect of eyestalk ablation on the precocious rematuration of the ovary. The higher number of atretic oocytes in the ovary of ablated females could be relevant to their reproductive performance...

On the origin of high growth rates in archosaurs and their ancient relatives: Complementary histological studies on Triassic archosauriforms and the problem of a “phylogenetic signal” in bone histology; Sur l’origine de taux de croissance élevés chez les archosauriens et formes anciennes apparentées : études histologiques complémentaires chez les archosauriformes triasiques et le problème d’un «signal phylogénétique» dans l’histologie osseuse

Ricqlès, Armand de; Padian, Kevin; Knoll, Fabien; Horner, John R.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 1735077 bytes; application/pdf
Português
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36.66%
20 pages, 13 figures, 1 table.-- Printed version published in the Apr-Jun 2008 issue.; [EN] Three possible hypotheses could explain the polarity of the histological features of basal archosauriform and archosauromorph reptiles: either, the fibrolamellar complex is basal; or, the lamellar-zonal complex is basal or finally, the condition varied, and each complex evolved more than once in these early groups. The answer to this question would have broad implications for our understanding of the physiological, ecological, and behavioral features of the first archosaurs. To this end, we sampled the bone histology of various archosauriforms and basal archosaurs from the Triassic and Lower Jurassic: erythrosuchids, proterochampsids, euparkeriids, and basal ornithischian dinosaurs, including forms close to the origin of archosaurs but poorly assessed phylogenetically. The new data suggest that the possibility of reaching and maintaining very high growth rates through ontogeny could have been a basal characteristic of archosauriforms. This was partly retained (at least during early ontogeny) in most lineages of Triassic pseudosuchians, which nevertheless generally relied on lower growth rates to reach large body sizes. This trend to slower growth seems to have been further emphasized among Crocodylomorpha...

Reconstructing life history traits from bone histology in extant and fossil ruminants

Marín Moratalla, Miren Nekane
Fonte: [Barcelona] : Universitat Autònoma de Barcelona, Publicador: [Barcelona] : Universitat Autònoma de Barcelona,
Tipo: Tesis i dissertacions electròniques; info:eu-repo/semantics/doctoralThesis; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em //2014 Português
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36.79%
El estudio de las life histories es de vital importancia porque proporciona evidencias sobre las condiciones ecológicas, biodiversidad, demografía, vulnerabilidad y otros muchos aspectos de la biología de las especies. La histología ósea es utilizada como herramienta para reconstruir las life histories de vertebrados, analizando el tejido óseo primario o contando el número de marcas de crecimiento (esqueletocronología). Sin embargo, se ha considerado que los endotermos, al contrario que en ectotermos, muestran un crecimiento óseo no cíclico, invalidando la histología ósea para inferir las life histories de mamíferos. El objetivo general de esta tesis es proporcionar las bases de la histología ósea en mamíferos para inferir estrategias de life history. Los objetivos concretos son: i) analizar la fiabilidad de la esqueletocronología ósea en mamíferos, ii) explorar la asociación entre las características del tejido óseo y el ambiente, fisiología, ontogenia y life history y, iii) reconstruir las características de life history en mamíferos fósiles y actuales para obtener datos sobre la evolución de las life histories y biología de la conservación. Se han analizado 274 láminas transversales de hueso de 225 individuos pertenecientes a lirones actuales (Gliridae) y rumiantes actuales y fósiles (Bovidae...

Correlation between macroscopic changes of arthrosis and the posterior cruciate ligament histology in the osteoarthritic knee

Stubbs, Geoffrey; Dahlstrom, Jane; Papantoniou, Peter; Cherian, Maya
Fonte: Blackwell Science Asia Publicador: Blackwell Science Asia
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.58%
Background: There is ongoing debate about the role of the posterior cruciate ligament (PCL) in total knee arthroplasty. Advocates of PCL retention cite better soft tissue balance and improved proprioception, whereas opponents report late flexion instability. The results of knee replacement are similar whether the PCL is retained or sacrificed. The aim of the present study was to examine the PCL for histological changes that would infer its competence and correlate these with changes easily observed by the operating surgeon. Methods: A prospective study of 50 osteoarthritic knees was performed. Results: Histology of the PCL showed changes secondary to degeneration and trauma. In most of the ligaments examined, arteriosclerosis and fibrosis were present. Half of the PCL examined showed perineural fibrosis, myxoid change and hyalinization. These changes, although very frequent, did not correlate well to the changes observed in either the anterior or PCL, or in the overall severity of osteoarthrosis. Conclusions: Posterior cruciate ligaments usually show degenerative and chronic traumatic change of varying degrees on histology. The changes are not predictable from inspection of the knee at surgery. The frequency of these changes suggests that many osteoarthritic PCL are of indifferent quality and the surgeon should consider this when choosing the style of knee replacement.

Diagnosis of Helicobacter pylori infection in patients with bleeding ulcer disease: rapid urease test and histology

Castro-Fernández,M.; Sánchez-Muñoz,D.; García-Díaz,E.; Miralles-Sanchiz,J.; Vargas-Romero,J.
Fonte: Revista Española de Enfermedades Digestivas Publicador: Revista Española de Enfermedades Digestivas
Tipo: info:eu-repo/semantics/article; journal article; info:eu-repo/semantics/publishedVersion Formato: text/html; application/pdf
Publicado em 01/06/2004 Português
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36.76%
Introduction: the endoscopic diagnosis of Helicobacter pylori infection in patients with bleeding peptic ulcer is limited by a decreased sensitivity in standard invasive tests, rapid urease test and histology. There is controversy about the convenience of using one, neither, or both diagnostic tests. Aims: to evaluate the results of simultaneously performed rapid urease test and histology in the diagnosis of Helicobacter pylori infection (H. pylori) in patients with bleeding peptic ulcer. Patients and methods: we included 173 patients, 98 male and 75 female, with an average age of 62 years (18-88), with upper gastrointestinal bleeding secondary to duodenal ulcer (115) or gastric ulcer (58), diagnosed within 24 hours after hospital admission. None of the patients had received treatment for H. pylori, proton pump inhibitors or antibiotics in the two weeks prior to the upper gastrointestinal bleeding episode. H. pylori infection was investigated in all patients by two antral biopsy samples for histological study (hematoxilin-eosin) and one or two antral biopsies for rapid urease test (Jatrox®-H.p.-test). In cases with a negative urease test and histology, a 13C urea breath test was performed. Infection was considered present when at least one invasive test or the breath test was positive...

Evaluation of the adequacy and diagnostic accuracy of the histology samples obtained with a newly designed 19-gauge EUS histology needle

Iglesias-García,Julio; Abdulkader,Ihab; Lariño-Noia,Jose; Domínguez-Muñoz,J. Enrique
Fonte: Revista Española de Enfermedades Digestivas Publicador: Revista Española de Enfermedades Digestivas
Tipo: info:eu-repo/semantics/article; journal article; info:eu-repo/semantics/publishedVersion Formato: text/html; application/pdf
Publicado em 01/01/2014 Português
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36.72%
Background: Endoscopic ultrasound (EUS)-guided fine needle aspiration (FNA) is an accurate technique for sampling intraintestinal and extraintestinal lesions. However, cytology possesses certain limitations, which may be overcome if histological specimens are provided to the pathologist. Aim: The aim of the study was to evaluate the accuracy of a newly developed 19G histology needle. Methods: Retrospective analysis of a prospectively collected data base including patients who underwent EUS-guided biopsy with the 19G ProCoreTM histology needle for the evaluation of intraintestinal or extraintestinal lesions. Samples were obtained after one needle pass, recovered into ThinPrep® and processed for histological analysis. Results were compared to the gold standard of surgical histopathology, or global pathological, clinical and radiological assessment, and follow-up in non-operated cases. Results are shown as mean ± SD. Percentage of optimal samples for histological evaluation and the overall diagnostic accuracy were evaluated. Results: 87 patients (mean age 62.9 years, range 25-88 years, 36 woman) were included. Lesions mean size was 41.6 ± 21.3 mm. 66 lesions (75.9 %) were considered as malignant and 21 (24.1 %) as benign. EUS-guided biopsy was feasible in all cases (100 %). Sample quality was adequate for histological assessment in 82 lesions (94.2 %). In the remaining cases the sample was adequate for cell-block evaluation. Sensitivity...

Histology of the parotid salivary gland of the african palm squirrel (epixerus ebii)

Ekele,Ikpegbu; Uchenna,Nlebedum; Okechukwu,Nnadozie; Isaiah,Agbakwuru
Fonte: Revista de la Facultad de Ciencias Veterinarias. Universidad Central de Venezuela. Publicador: Revista de la Facultad de Ciencias Veterinarias. Universidad Central de Venezuela.
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2013 Português
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36.66%
The parotid salivary gland microscopic morphology of the adult African Palm squirrel (Epixerus ebii) was investigated. This study was carried out to provide the basic histology of this organ in the palm squirrel, as there is dearth of information of its microscopic morphology from available literature. This becomes more important as the increasing use of this species of rodent as a pet and animal of importance in African game reserves for tourist attraction. The possibility of its domestication as a ready source of animal protein is also growing. Hence the need to understand the digestive biology to help animal nutritionist in feed formulation. The histology revealed the presence of only serous secretory acini cells. The serous cells were triangular to polyhedral shaped with a roundish basally located nucleus inside the mostly pinkish granulated cytoplasm. Myoepithelial cells were seen around the secretory cells and the intercalated ducts. The intralobular ducts of intercalated and striated ducts were lined by simple cuboidal and simple columnar cells, respectively. The excretory duct seen in the surrounding connective tissue was lined by stratified cuboidal cells. The granules in the cytoplasm of serous cells must be the digestive enzyme amylase that initiates carbohydrate digestion. This well developed serous parotid salivary gland reflects the need for efficient carbohydrate digestion since the animal forages on fruits and nuts that are rich in carbohydrates. The granules could also contain lysozymes which serve in local defense against pathogenic agents in the oral cavity. This study for the first time documents the normal histology of the parotid salivary gland in this species...