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Characterization of electrical penetration graphs of Bucephalogonia xanthophis, a vector of Xylella fastidiosa in citrus

MIRANDA, M. P.; FERERES, A.; APPEZZATO-DA-GLORIA, B.; LOPES, J. R. S.
Fonte: WILEY-BLACKWELL PUBLISHING, INC Publicador: WILEY-BLACKWELL PUBLISHING, INC
Tipo: Artigo de Revista Científica
Português
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26.83%
The sharpshooter Bucephalogonia xanthophis (Berg) (Homoptera: Cicadellidae) is a vector of the xylem-limited bacterium, Xylella fastidiosa (Wells, Raju, Hung, Weisburg, Mandelco-Paul, and Brenner), which causes citrus variegated chlorosis. Despite the importance of citrus variegated chlorosis, the probing behavior of vectors on citrus and its implications for transmission of X. fastidiosa have not been studied. Here we studied electrical penetration graph (EPG-DC system) waveforms produced by B. xanthophis on Citrus sinensis (L.) Osbeck (Rutaceae), and their relationships with stylet activities and xylem ingestion. Electrical penetration graph waveforms were described based on amplitude, frequency, voltage level, and electrical origin of the observed traces during stylet penetration on plant tissues. The main waveforms were correlated with histological observations of salivary sheaths in plant tissues and excretion analysis, in order to determine stylet activities and their precise position. Six waveforms and associated activities are described: (S) secretion of salivary sheath and intracellular stylet pathway, (R) resting during stylet pathway, (Xc) contact of stylets with xylem vessels, (Xi) active xylem ingestion, (N) interruption within the xylem phase (during Xc or Xi)...

Estudos básicos para a utilização de plantio-isca visando ao controle de cigarrinhas vetoras de Xylella fastidiosa em pomares cítricos; Establishment of a trap cropping strategy for controlling sharpshooter vectors of Xylella fastidiosa in citrus groves

Marques, Rodrigo Neves
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 24/01/2007 Português
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26.68%
Clorose Variegada dos Citros (CVC), causada pela bactéria Xylella fastidiosa Wells et al., tem causado grandes perdas na citricultura do estado de São Paulo, afetando cerca de 40% das plantas de laranja doce. A bactéria é transmitida por 12 espécies de cigarrinhas sugadoras de xilema (Hemiptera: Cicadellidae) da subfamília Cicadellinae. O manejo da CVC é baseado no plantio de mudas sadias, poda ou eliminação de árvores infectadas no campo, assim como o controle químico dos vetores. No entanto, as cigarrinhas vetoras possuem hábitats e hospedeiros alternativos que servem como refúgio, particularmente na vegetação adjacente aos pomares cítricos. Esta característica ecológica explica a rápida reinfestação dos pomares de laranja pelas cigarrinhas após a aplicação de inseticidas. O uso de plantas hospedeiras atrativas às cigarrinhas em uma estratégia de plantio-isca poderia reduzir a imigração de vetores da vegetação adjacente. O objetivo deste trabalho foi selecionar plantas hospedeiras adequadas para serem usadas neste tipo de estratégia, assim como testar a eficácia do plantio-isca em reduzir a população de cigarrinhas em um pomar de laranja. Experimentos de livre escolha foram conduzidos sob condições de casa-de-vegetação para avaliar a preferência de dois importantes vetores...

Feeding site preference of Dilobopterus costalimai Young and Oncometopia facialis (Signoret) (Hemiptera: Cicadellidae) on citrus plants

Marucci,Rosangela C.; Lopes,João R.S.; Vendramim,José D.; Corrente,José E.
Fonte: Sociedade Entomológica do Brasil Publicador: Sociedade Entomológica do Brasil
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2004 Português
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37.01%
The efficiency of Xylella fastidiosa transmission by sharpshooters on citrus plants is low and varies with the vector species. The feeding behavior of the sharpshooter vector related to X. fastidiosa acquisition and inoculation is one of the probable conditioning factors in transmission efficiency. The objective of this study was to assess the behavior of two sharpshooter species, Dilobopterus costalimai Young and Oncometopia facialis (Signoret), in selection of citrus nursery trees and feeding places and to ascertain the influence of sprouting on plant attractiveness to the vectors. Free choice tests were performed with the two species in observation chambers (63 x 63 x 120 cm), releasing 40 individuals per chamber. The number of dead insects were assessed as well as those that chose, or not, one of the treatments, at 3, 15, 21, 24, 39, 45 and 48h after the release. For D. costalimai the leaves were important at the start of host selection, and were substituted over time by the secondary branch. D. costalimai preferred to stay on the secondary leaf nervures followed by the secondary branch. O. facialis showed no difference in preference for staying on the leaves (central and secondary nervures) and secondary branch on citrus plants...

Symbiosis and Insect Diversification: an Ancient Symbiont of Sap-Feeding Insects from the Bacterial Phylum Bacteroidetes

Moran, Nancy A.; Tran, Phat; Gerardo, Nicole M.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /12/2005 Português
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26.21%
Several insect groups have obligate, vertically transmitted bacterial symbionts that provision hosts with nutrients that are limiting in the diet. Some of these bacteria have been shown to descend from ancient infections. Here we show that the large group of related insects including cicadas, leafhoppers, treehoppers, spittlebugs, and planthoppers host a distinct clade of bacterial symbionts. This newly described symbiont lineage belongs to the phylum Bacteroidetes. Analyses of 16S rRNA genes indicate that the symbiont phylogeny is completely congruent with the phylogeny of insect hosts as currently known. These results support the ancient acquisition of a symbiont by a shared ancestor of these insects, dating the original infection to at least 260 million years ago. As visualized in a species of spittlebug (Cercopoidea) and in a species of sharpshooter (Cicadellinae), the symbionts have extraordinarily large cells with an elongate shape, often more than 30 μm in length; in situ hybridizations verify that these correspond to the phylum Bacteroidetes. “Candidatus Sulcia muelleri” is proposed as the name of the new symbiont.

Potentially Hygroreceptive Sensilla on the Anal Stylus of the Glassy-Winged Sharpshooter, Homalodisca vitripennis

Hummel, Natalie A.; Leal, Walter S.; Zalom, Frank G.
Fonte: University of Wisconsin Library Publicador: University of Wisconsin Library
Tipo: Artigo de Revista Científica
Publicado em 13/10/2008 Português
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26.75%
This study begins to elucidate the cues and mechanisms by which the glassy-winged sharpshooter, Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), select host plants for feeding and oviposition. The electrophysiological response of the anal styli of male and female H. vitripennis to water vapor was examined using a modified electroantennography (EAG) device (stylogram). A strong electrophysiological response of the anal stylus to water vapor was found. Scanning electron microscopic examination of the anal stylus revealed the presence of long mechanosensory hairs, many small coeloconic-type sensilla, and masses of secretory granules termed brochosomes. Each coeloconic sensillum is located in a pit from which protrude finger-like projections. The pit is often blocked by masses of brochosomes and an unidentified dense material. Based on the electrophysiological response of the anal stylus to water vapor, we hypothesize that the coeloconic sensilla on the stylus may be hygroreceptors. H. vitripennis are xylem feeders and may use the sensilla to assist in host selection for the purpose of feeding or oviposition based on detected plant water status. Furthermore, H. vitripennis oviposit into the leaf epidermis, and may use these sensilla to evaluate moisture content to determine host suitability for both oviposition and subsequent feeding of emerged progeny. Understanding the cues and underlying mechanisms of host selection is an important consideration for predicting the movement of H. vitripennis between crops and disease epidemiology.

Formation of Stylet Sheaths in āere (in air) from Eight Species of Phytophagous Hemipterans from Six Families (Suborders: Auchenorrhyncha and Sternorrhyncha)

Morgan, J. Kent; Luzio, Gary A.; Ammar, El-Desouky; Hunter, Wayne B.; Hall, David G.; Shatters Jr, Robert G.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 24/04/2013 Português
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16.52%
Stylet sheath formation is a common feature among phytophagous hemipterans. These sheaths are considered essential to promote a successful feeding event. Stylet sheath compositions are largely unknown and their mode of solidification remains to be elucidated. This report demonstrates the formation and solidification of in āere (in air) produced stylet sheaths by six hemipteran families: Diaphorina citri (Psyllidae, Asian citrus psyllid), Aphis nerii (Aphididae, oleander/milkweed aphid), Toxoptera citricida (Aphididae, brown citrus aphid), Aphis gossypii (Aphididae, cotton melon aphid), Bemisia tabaci biotype B (Aleyrodidae, whitefly), Homalodisca vitripennis (Cicadellidae, glassy-winged sharpshooter), Ferrisia virgata (Pseudococcidae, striped mealybug), and Protopulvinaria pyriformis (Coccidae, pyriform scale). Examination of in āere produced stylet sheaths by confocal and scanning electron microscopy shows a common morphology of an initial flange laid down on the surface of the membrane followed by continuous hollow core structures with sequentially stacked hardened bulbous droplets. Single and multi-branched sheaths were common, whereas mealybug and scale insects typically produced multi-branched sheaths. Micrographs of the in āere formed flanges indicate flange sealing upon stylet bundle extraction in D. citri and the aphids...

Feeding and Development of the Glassy-Winged Sharpshooter, Homalodisca vitripennis, on Australian Native Plant Species and Implications for Australian Biosecurity

Rathé, Anna A.; Pilkington, Leigh J.; Hoddle, Mark S.; Spohr, Lorraine J.; Daugherty, Matthew P.; Gurr, Geoff M.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 10/03/2014 Português
Relevância na Pesquisa
26.67%
In any insect invasion the presence or absence of suitable food and oviposition hosts in the invaded range is a key factor determining establishment success. The glassy-winged sharpshooter, Homalodisca vitripennis, is an important insect vector of the xylem-limited bacterial plant pathogen, Xylella fastidiosa, which causes disease in numerous host plants including food and feedstock crops, ornamentals and weeds. Both the pathogen and the vector are native to the Americas and are considered to be highly invasive. Neither has been detected in Australia. Twelve Australian native plant species present in the USA were observed over two years for suitability as H. vitripennis feeding, oviposition and nymph development hosts. Hosts providing evidence of adult or nymph presence were Leptospermum laevigatum, Acacia cowleana, Eremophila divaricata, Eucalyptus wandoo, Hakea laurina, Melaleuca laterita and Swainsona galegifolia. An oviposition-suitability field study was conducted with citrus, a favoured oviposition host, as a positive control. Citrus and L. laevigatum, A. cowleana, B. ericifolia×B. spinulosa, C. pulchella, E. divaricata, E. wandoo, H. laurina, and S. galegifolia were found to be oviposition hosts. Egg parasitism by the mymarid parasitoid Gonatocerus ashmeadi was observed on all Australian plants. A number of Australian plants that may facilitate H. vitripennis invasion have been identified and categorised as ‘high risk’ due to their ability to support all three life stages (egg...

Locais e período de alimentação da cigarrinha vetora de Xylella fastidiosa, Bucephalogonia xanthophis (Berg) (Hemiptera: Cicadellidae), em mudas cítricas; Feeding sites and food intake of Bucephalogonia xanthophis (Berg) (Hemiptera: Cicadellidae), a sharpshooter vector of Xylella fastidiosa, on citrus plants

MIRANDA, Marcelo Pedreira de; VIOLA, Denise Nunes; MARQUES, Rodrigo Neves; BONANI, Jean Patrick; LOPES, João Roberto Spotti
Fonte: Sociedade Brasileira de Fruticultura Publicador: Sociedade Brasileira de Fruticultura
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.89%
A cigarrinha Bucephalogonia xanthophis (Berg) (Hemiptera: Cicadellidae) é um importante vetor da bactéria Xylella fastidiosa, agente causal da clorose variegada dos citros. Este trabalho teve como objetivo identificar o local preferido de alimentação e o período de maior atividade alimentar desta cigarrinha em citros, no sentido de elucidar o comportamento alimentar relacionado à transmissão da bactéria. O local de alimentação foi estudado em ensaio de escolha, no qual 30 insetos adultos foram liberados em gaiolas de observação (n = 10) contendo uma muda de laranja-doce [Citrus sinensis (L.) Osbeck]. Após 1; 15; 21; 25; 39; 45 e 49 h da liberação, contaram-se os insetos na parte superior (ramos com brotações) e inferior (haste principal, até H40 cm de altura) da muda. Nos ramos da parte superior, avaliou-se a preferência entre a haste, o pecíolo e o limbo foliar. Em um segundo ensaio, 20 machos e 20 fêmeas de B. xanthophis foram confinados individualmente sobre a haste de 'seedlings' de laranja-doce para determinar os períodos de alimentação, quantificando-se a excreção de 'honeydew' (medida indireta da ingestão) em períodos sucessivos de dia e noite, durante 48 h. A maioria dos indivíduos de B. xanthophis preferiu a haste dos ramos novos (62%)...

Detection of the bacterium, Xylella fastidiosa, in saliva of glassy-winged sharpshooter, Homalodisca vitripennis

RAMIREZ, Jose L.; LACAVA, Paulo T.; MILLER, Thomas A.
Fonte: UNIV ARIZONA Publicador: UNIV ARIZONA
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
46.91%
Homalodisca vitripennis ( Germar) ( Hemiptera: Cicadellidae), the glassy- winged sharpshooter, is one of the most important vectors of the bacterium, Xylella fastidiosa subsp. piercei ( Xanthomonadales: Xanthomonadaceae) that causes Pierce's Disease in grapevines in California. In the present study we report a new method for studying pathogen transmission or probing behavior of H. vitripennis. When confined, H. vitripennis attempt to probe the surface of sterile containers 48 hours post- acquisition of X. f. piercei. The saliva deposited during attempted feeding probes was found to contain X. f. piercei. We observed no correlation between X. f. piercei titers in the foregut of H. vitripennis that fed on Xylella- infected grapevines and the presence of this bacterium in the deposited saliva. The infection rate after a 48 h post- acquisition feeding on healthy citrus and grapevines was observed to be 77% for H. vitripennis that fed on grapevines and 81% for H. vitripennis that fed on citrus, with no difference in the number of positive probing sites from H. vitripennis that fed on either grapevine or citrus. This method is amenable for individual assessment of X. f. piercei- infectivity, with samples less likely to be affected by tissue contamination that is usually present in whole body extracts.

Survey of potential sharpshooter and spittlebug vectors of Xylella fastidiosa to grapevines at the São Francisco River Valley, Brazil

Ringenberg,Rudiney; Lopes,João Roberto Spotti; Müller,Cristiane; Azevedo-Filho,Wilson Sampaio de; Paranhos,Beatriz Aguiar Jordão; Botton,Marcos
Fonte: Sociedade Brasileira De Entomologia Publicador: Sociedade Brasileira De Entomologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2014 Português
Relevância na Pesquisa
26.68%
Survey of potential sharpshooter and spittlebug vectors of Xylella fastidiosa to grapevines at the São Francisco River Valley, Brazil. Pierce's disease of grapevines, caused by Xylella fastidiosa, is a serious problem in some regions of North America, not yet reported in Brazil. In this study, a survey of potential sharpshooter (Hemiptera, Cicadellidae, Cicadellinae) and spittlebug (Hemiptera, Cercopidae) vectors of X. fastidiosa was conducted in vineyards at the São Francisco River Valley, a major grape growing region in Brazil. Four vineyards of Vitis vinifera L. were sampled fortnightly from June/2005 to June/2007, using yellow sticky cards, each placed at two different heights (45 cm aboveground and 45 cm above the crop canopy) in 10 sampling localities. A total of 4,095 specimens of sharpshooters were collected, nearly all from 3 Proconiini species, Homalodisca spottii Takiya, Cavichioli & McKamey, 2006 (96.8% of the specimens), Tapajosa fulvopunctata (Signoret, 1854) (3.1%), and Tretogonia cribrata Melichar, 1926 (1 specimen). Hortensia similis (Walker, 1851) (2 specimens) was the only Cicadellini species. Only 1 cercopid specimen, belonging to Aeneolamia colon (Germar, 1821), was trapped. Even though they are not considered potential Xylella vectors...

Impact of pymetrozine on glassy-winged sharpshooter feeding behavior and rate of Xylella fastidiosa transmission

Bextine, B.R.; Harshman, D.; Johnson, M.C.; Miller, T.A.
Fonte: University of Arizona Library Publicador: University of Arizona Library
Tipo: Artigo de Revista Científica
Publicado em 22/10/2004 Português
Relevância na Pesquisa
36.75%
Pymetrozine is a compound that interferes with insect feeding and interrupts transmission of plant pathogens. The glassy-winged sharpshooter, Homalodisca coagulata Say (Hemiptera, Cicadellidae), is a vector of Xylella fastidiosa, the foregut-borne, propagative bacterium that causes Pierce's disease of grapevine. In this study, we recorded the behavioral response of H. coagulata to plants treated by soil drench with pymetrozine using time-lapse photography, quantified the reduction in liquid excreta produced by H. coagulata fed on pymetrozine-treated plants, and evaluated pymetrozine effectiveness in reducing transmission rate in grapevines. H. coagulata feeding on plants treated with 0.015 mg of pymetrozine was disrupted by decreasing the number of contacts made with the grapevine by more than 50% and by increasing movements away from the stem by more than 5-fold. Excreta production by H. coagulata was significantly reduced on plants treated with 0.015 or 0.0075 mg of pymetrozine. Contrary to the expected outcome, the mean number of X. fastidiosa-infected plants actually increased in the pymetrozine treatments relative to the controls.

Characterization of Hovi-mEH1, a microsomal epoxide hydrolase from the glassy-winged sharpshooter Homalodisca vitripennis

Kamita, Shizuo G.; Oshita, Grant H.; Wang, Peng; Nandety, Raja Sekhar; Morisseau, Christophe; Falk, Bryce W.; Hammock, Bruce D.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
26.52%
Epoxide hydrolase (EH) is an enzyme in the α/β-hydrolase fold superfamily that uses a water molecule to transform an epoxide to its corresponding diol. In insects, EHs metabolize among other things critical developmental hormones called juvenile hormones (JHs). EHs also play roles in the detoxification of toxic compounds that are found in the insect's diet or environment. In this study, a full-length cDNA encoding an epoxide hydrolase, Hovi-mEH1, was obtained from the xylem-feeding insect Homalodisca vitripennis. H. vitripennis, commonly known as the glassy-winged sharpshooter, is an economically important vector of plant pathogenic bacteria such as Xylella fastidiosa. Hovi-mEH1 hydrolyzed the general EH substrates cis-stilbene oxide and trans-diphenylpropene oxide with specific activities of 47.5 ± 6.2 and 1.3 ± 0.5 nmol of diol formed min−1 mg−1, respectively. Hovi-mEH1 metabolized JH III with a Vmax of 29.3 ± 1.6 nmol min−1 mg−1, kcat of 0.03 s−1, and KM of 13.8 ± 2.0 μM. These Vmax and kcat values are similar to those of known JH metabolizing EHs (JHEHs) from lepidopteran and coleopteran insects. Hovi-mEH1 showed 99.1% identity to one of three predicted EH-encoding sequences that were identified in the transcriptome of H. vitripennis. Of these three sequences only Hovi-mEH1 clustered with known JHEHs. On the basis of biochemical...

Detection of the Bacterium, Xylella fastidiosa, in Saliva of Glassy-Winged Sharpshooter, Homalodisca vitripennis

Ramirez, Jose L.; Lacava, Paulo T.; Miller, Thomas A.
Fonte: University of Wisconsin Library Publicador: University of Wisconsin Library
Tipo: Artigo de Revista Científica
Publicado em 29/04/2008 Português
Relevância na Pesquisa
26.67%
Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), the glassy-winged sharpshooter, is one of the most important vectors of the bacterium, Xylella fastidiosa subsp. piercei (Xanthomonadales: Xanthomonadaceae) that causes Pierce's Disease in grapevines in California. In the present study we report a new method for studying pathogen transmission or probing behavior of H. vitripennis. When confined, H. vitripennis attempt to probe the surface of sterile containers 48 hours post-acquisition of X. f. piercei. The saliva deposited during attempted feeding probes was found to contain X. f. piercei. We observed no correlation between X. f. piercei titers in the foregut of H. vitripennis that fed on Xylella-infected grapevines and the presence of this bacterium in the deposited saliva. The infection rate after a 48 h post-acquisition feeding on healthy citrus and grapevines was observed to be 77% for H. vitripennis that fed on grapevines and 81% for H. vitripennis that fed on citrus, with no difference in the number of positive probing sites from H. vitripennis that fed on either grapevine or citrus. This method is amenable for individual assessment of X. f. piercei-infecuvity, with samples less likely to be affected by tissue contamination that is usually present in whole body extracts.

Identification of Novel and Conserved microRNAs in Homalodisca vitripennis, the Glassy-Winged Sharpshooter by Expression Profiling

Nandety, Raja Sekhar; Sharif, Almas; Kamita, Shizuo G.; Ramasamy, Asokan; Falk, Bryce W.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 06/10/2015 Português
Relevância na Pesquisa
26.52%
The glassy-winged sharpshooter (GWSS) Homalodisca vitripennis (Hemiptera: Cicadellidae), is a xylem-feeding leafhopper and an important vector of the bacterium Xylella fastidiosa; the causal agent of Pierce’s disease of grapevines. MicroRNAs are a class of small RNAs that play an important role in the functional development of various organisms including insects. In H. vitripennis, we identified microRNAs using high-throughput deep sequencing of adults followed by computational and manual annotation. A total of 14 novel microRNAs that are not found in the miRBase were identified from adult H. vitripennis. Conserved microRNAs were also found in our datasets. By comparison to our previously determined transcriptome sequence of H. vitripennis, we identified the potential targets of the microRNAs in the transcriptome. This microRNA profile information not only provides a more nuanced understanding of the biological and physiological mechanisms that govern gene expression in H. vitripennis, but may also lead to the identification of novel mechanisms for biorationally designed management strategies through the use of microRNAs.

Characterization of electrical penetration graphs of Bucephalogonia xanthophis, a vector of Xylella fastidiosa in citrus

Miranda, M. P.; Fereres, Alberto; Appezzato-da-Gloria, B.; Lopes, J. R. S.
Fonte: Netherlands Entomological Society Publicador: Netherlands Entomological Society
Tipo: Artículo Formato: 1042648 bytes; application/pdf
Português
Relevância na Pesquisa
26.68%
12 pages, and tables.; The sharpshooter Bucephalogonia xanthophis (Berg) (Homoptera: Cicadellidae) is a vector of the xylemlimited bacterium, Xylella fastidiosa (Wells, Raju, Hung, Weisburg, Mandelco-Paul, and Brenner), which causes citrus variegated chlorosis. Despite the importance of citrus variegated chlorosis, the probing behavior of vectors on citrus and its implications for transmission of X. fastidiosa have not been studied. Here we studied electrical penetration graph (EPG-DC system) waveforms produced by B. xanthophis on Citrus sinensis (L.) Osbeck (Rutaceae), and their relationships with stylet activities and xylem ingestion. Electrical penetration graph waveforms were described based on amplitude, frequency, voltage level, and electrical origin of the observed traces during stylet penetration on plant tissues. The main waveforms were correlated with histological observations of salivary sheaths in plant tissues and excretion analysis, in order to determine stylet activities and their precise position. Six waveforms and associated activities are described: (S) secretion of salivary sheath and intracellular stylet pathway, (R) resting during stylet pathway, (Xc) contact of stylets with xylem vessels, (Xi) active xylem ingestion...

Karyotype and male pre-reductional meiosis of the sharpshooter Tapajosa rubromarginata (Hemiptera: Cicadellidae)

de Bigliardo,Graciela R; Virla,Eduardo Gabriel; Caro,Sara; Murillo Dasso,Santiago
Fonte: Revista de Biología Tropical Publicador: Revista de Biología Tropical
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2011 Português
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26.52%
Cicadellidae in one of the best represented families in the Neotropical Region, and the tribe Proconiini comprises most of the xylem-feeding insects, including the majority of the known vectors of xylem-born phytopathogenic organisms. The cytogenetics of the Proconiini remains largely unexplored. We studied males of Tapajosa rubromarginata (Signoret) collected at El Manantial (Tucumán, Argentina) on native spontaneous vegetation where Sorghum halepense predominates. Conventional cytogenetic techniques were used in order to describe the karyotype and male meiosis of this sharpshooter. T. rubromarginata has a male karyological formula of 2n=21 and a sex chromosome system XO:XX (♂:♀). The chromosomes do not have a primary constriction, being holokinetic and the meiosis is pre-reductional, showing similar behavior both for autosomes and sex chromosomes during anaphase I. For this stage, chromosomes are parallel to the acromatic spindle with kinetic activities in the telomeres. They segregate reductionally in the anaphase I, and towards the equator during the second division of the meiosis. This is the first contribution to cytogenetic aspects on proconines sharpshooters, particularly on this economic relevant Auchenorrhyncha species. Rev. Biol. Trop. 59 (1): 309-314. Epub 2011 March 01.